Identification of mRNA transcripts and immunohistochemical localization of Na/H exchanger isoforms in gerbil inner ear.

Abstract:

:Recent physiological and pharmacological studies have implicated involvement of the Na/H exchanger (NHE) in regulating inner ear ion homeostasis, but the cellular distribution of this membrane transporter remains unknown. Here reverse transcription and the polymerase chain reaction (RT-PCR) were employed to screen adult gerbil inner ears for mRNA transcripts encoding the four best characterized isoforms of NHE. PCR products spanning selected segments of NHE mRNAs were cloned and sequenced. The putative housekeeping gene NHE-1 was found to be expressed and the 459 bp product shared 98.7% amino acid homology with rat sequence. NHE-2, NHE-3 and NHE-4 cDNA transcripts likewise were detected and the PCR products shared 100, 99.4 and 88.9% amino acid homology, respectively, with their rat counterparts. In addition, the cellular distribution of NHE isoforms 1 and 3 was mapped in the gerbil inner ear by immunostaining with polyclonal antisera against rat antigens. In the cochlea, the antiserum against NHE-1 reacted strongly at the basolateral membrane of strial marginal cells as well as with inner and outer hair cells and spiral ganglion neurons. Less intense staining for NHE-1 was present in subpopulations of fibrocytes in the spiral limbus and in inferior and superior areas of the spiral ligament. In the vestibular system dark and transitional cells expressed abundant NHE-1 as did hair cells and vestibular ganglia neurons. Immunostaining with the antiserum against NHE-3 was limited to the apical surface of marginal cells in the stria vascularis. Based on these data, NHE-1 likely functions primarily to maintain intracellular pH levels in cells where it is found in high abundance. NHE-3, on the other hand, possibly participates in the vectorial transcellular movement of Na+ by strial marginal cells thus helping to maintain the extremely low Na+ level in cochlear endolymph.

journal_name

Hear Res

journal_title

Hearing research

authors

Bond BR,Ng LL,Schulte BA

doi

10.1016/s0378-5955(98)00089-6

subject

Has Abstract

pub_date

1998-09-01 00:00:00

pages

1-9

issue

1-2

eissn

0378-5955

issn

1878-5891

pii

S0378-5955(98)00089-6

journal_volume

123

pub_type

杂志文章
  • Effect of modulation of basilar membrane position on the cochlear microphonic.

    abstract::Certain characteristics of the extracellular cochlear microphonic (CM) recorded by intracochlear electrodes change in a bimodal manner as a function of prior acoustic exposure, intensity of stimulation, or stimulus frequency. In the present study, it is shown that biasing the basilar membrane position toward scala tym...

    journal_title:Hearing research

    pub_type: 杂志文章

    doi:10.1016/0378-5955(80)90036-2

    authors: Pierson M,Møller A

    更新日期:1980-03-01 00:00:00

  • A comparison of the effects of isoflurane and ketamine anesthesia on auditory brainstem response (ABR) thresholds in rats.

    abstract::The auditory brainstem response (ABR) is an acoustically evoked potential commonly used to determine hearing sensitivity in laboratory animals. Both isoflurane and ketamine/xylazine anesthesia are commonly used to immobilize animals during ABR procedures. Hearing threshold determination is often the primary interest. ...

    journal_title:Hearing research

    pub_type: 杂志文章

    doi:10.1016/j.heares.2012.04.005

    authors: Ruebhausen MR,Brozoski TJ,Bauer CA

    更新日期:2012-05-01 00:00:00

  • The responses of inner and outer hair cells in the basal turn of the guinea-pig cochlea and in the mouse cochlea grown in vitro.

    abstract::Until recently the responses of the mechanosensitive hair cells of the cochlea have been inferred from their morphology, morphological relationships with other structures in the cochlea, and by indirect electrophysiological measurements. With the advent of techniques for making intracellular recordings from hair cells...

    journal_title:Hearing research

    pub_type: 杂志文章

    doi:10.1016/0378-5955(86)90096-1

    authors: Russell IJ,Cody AR,Richardson GP

    更新日期:1986-01-01 00:00:00

  • Calcification processes in the chick otoconia and calcium binding proteins: patterns of tetracycline incorporation and calbindin-D28K distribution.

    abstract::In order to clarify the otoconia formation and turnover, tetracycline, an antibiotic that precipitates at calcifying fronts and serves as a fluorescent marker, was injected into eggs at different stages of chick embryonic development, as well as into postnatal chicken and into adult animals. The changes in the intensi...

    journal_title:Hearing research

    pub_type: 杂志文章

    doi:10.1016/s0378-5955(00)00094-0

    authors: Balsamo G,Avallone B,Del Genio F,Trapani S,Marmo F

    更新日期:2000-10-01 00:00:00

  • Increase in glutamate-aspartate transporter (GLAST) mRNA during kanamycin-induced cochlear insult in rats.

    abstract::Kanamycin (KM)-induced changes in expression of the gene for glutamate-aspartate transporter (GLAST) in the rat cochlea were analyzed by Northern blotting. With the administration of KM (600 mg/kg/day) once daily for 20 days, the expression of GLAST mRNA gradually increased and reached a peak on day 20. Although the e...

    journal_title:Hearing research

    pub_type: 杂志文章

    doi:10.1016/s0378-5955(99)00050-7

    authors: Matsuda K,Ueda Y,Doi T,Tono T,Haruta A,Toyama K,Komune S

    更新日期:1999-07-01 00:00:00

  • Development of auditory function in the tammar wallaby Macropus eugenii.

    abstract::Auditory brainstem responses (ABRs) were evoked in developing wallabies by click and tone burst stimuli delivered by bone conduction and air conduction, at progressive stages of post-natal (pouch) life. ABRs were recorded through the onset of auditory responses (95-110 days), the opening of the external ear canal (125...

    journal_title:Hearing research

    pub_type: 杂志文章

    doi:10.1016/s0378-5955(97)00211-6

    authors: Hill KG,Cone-Wesson B,Liu GB

    更新日期:1998-03-01 00:00:00

  • Morphometric changes in the cochlear nucleus in patients who had undergone cochlear implantation for bilateral profound deafness.

    abstract::We have investigated the morphometric changes in the cochlear nucleus of patients who had undergone cochlear implantation following profound deafness. The brain stems of 11 adult patients who had undergone implantation and four non-implanted control cases with varying degrees of hearing loss were studied. The volumes ...

    journal_title:Hearing research

    pub_type: 杂志文章

    doi:10.1016/s0378-5955(02)00694-9

    authors: Chao TK,Burgess BJ,Eddington DK,Nadol JB Jr

    更新日期:2002-12-01 00:00:00

  • Elasticity modulus of rabbit middle ear ossicles determined by a novel micro-indentation technique.

    abstract::For the purpose of creating a finite element model of the middle ear, the ossicles can be modelled as rigid bodies or as linear elastic materials. The general elasticity parameters used are usually measured on larger bones like the femur. In order to obtain a highly realistic model, the actual elastic modulus (Young's...

    journal_title:Hearing research

    pub_type: 杂志文章

    doi:10.1016/j.heares.2009.10.001

    authors: Soons JA,Aernouts J,Dirckx JJ

    更新日期:2010-05-01 00:00:00

  • Objective evidence of tinnitus in auditory evoked magnetic fields.

    abstract::The waveforms of the auditory evoked magnetic field in normal-hearing individuals and patients suffering from tinnitus are distinctly different. In tinnitus patients, the magnetic wave M200 (corresponding to the electric wave P200, or P2) is delayed and only poorly developed or even completely missing, while the ampli...

    journal_title:Hearing research

    pub_type: 杂志文章

    doi:10.1016/0378-5955(89)90028-2

    authors: Hoke M,Feldmann H,Pantev C,Lütkenhöner B,Lehnertz K

    更新日期:1989-02-01 00:00:00

  • Anemone repair proteins as a potential therapeutic agent for vertebrate hair cells: facilitated recovery of the lateral line of blind cave fish.

    abstract::Blind cave fish use the lateral line sensory system to detect nearby objects. The fish responds to sudden perturbations in the water column by initiating startle responses in which they swim more rapidly. Normal startle responses disappear after trauma caused by a single 15 s immersion in calcium free water, but retur...

    journal_title:Hearing research

    pub_type: 杂志文章

    doi:10.1016/s0378-5955(01)00226-x

    authors: Repass JJ,Watson GM

    更新日期:2001-04-01 00:00:00

  • The response of guinea pig primary utricular and saccular irregular neurons to bone-conducted vibration (BCV) and air-conducted sound (ACS).

    abstract:UNLABELLED:This study sought to characterize the response of mammalian primary otolithic neurons to sound and vibration by measuring the resting discharge rates, thresholds for increases in firing rate and supra-threshold sensitivity functions of guinea pig single primary utricular and saccular afferents. Neurons with ...

    journal_title:Hearing research

    pub_type: 杂志文章

    doi:10.1016/j.heares.2015.10.019

    authors: Curthoys IS,Vulovic V,Burgess AM,Sokolic L,Goonetilleke SC

    更新日期:2016-01-01 00:00:00

  • Micro-channels in the mastoid anatomy. Indications of a separate blood supply of the air cell system mucosa by micro-CT scanning.

    abstract::The mastoid air cell system has traditionally been considered to have a passive role in gas exchange and pressure regulation of the middle ear possibly with some acoustic function. However, more evidence has focused on the mucosa of the mastoid, which may play a more active role in regulation of middle ear pressure. I...

    journal_title:Hearing research

    pub_type: 杂志文章

    doi:10.1016/j.heares.2013.03.002

    authors: Cros O,Borga M,Pauwels E,Dirckx JJ,Gaihede M

    更新日期:2013-07-01 00:00:00

  • Masculinization of the mammalian cochlea.

    abstract::Otoacoustic emissions (OAEs) differ between the sexes in humans, rhesus and marmoset monkeys, and sheep. OAEs also are different in a number of special populations of humans. Those basic findings are reviewed and discussed in the context of possible prenatal-androgen effects on the auditory system. A parsimonious expl...

    journal_title:Hearing research

    pub_type: 杂志文章,评审

    doi:10.1016/j.heares.2009.01.002

    authors: McFadden D

    更新日期:2009-06-01 00:00:00

  • Neural sensitivity to statistical regularities as a fundamental biological process that underlies auditory learning: the role of musical practice.

    abstract::There is increasing evidence that humans and other nonhuman mammals are sensitive to the statistical structure of auditory input. Indeed, neural sensitivity to statistical regularities seems to be a fundamental biological property underlying auditory learning. In the case of speech, statistical regularities play a cru...

    journal_title:Hearing research

    pub_type: 杂志文章,评审

    doi:10.1016/j.heares.2013.08.018

    authors: François C,Schön D

    更新日期:2014-02-01 00:00:00

  • Sound damage and gentamicin treatment produce different patterns of damage to the efferent innervation of the chick cochlea.

    abstract::Both sound exposure and gentamicin treatment cause damage to sensory hair cells in the peripheral chick auditory organ, the basilar papilla. This induces a regeneration response which replaces hair cells and restores auditory function. Since functional recovery requires the re-establishment of connections between rege...

    journal_title:Hearing research

    pub_type: 杂志文章

    doi:10.1016/s0378-5955(97)00150-0

    authors: Ofsie MS,Hennig AK,Messana EP,Cotanche DA

    更新日期:1997-11-01 00:00:00

  • Latency enhancement of the cochlear nerve compound action potential (CAP).

    abstract::Forward maskers within two frequency-intensity domains are capable of decreasing (enhancing) CAP latency: one region flanks the low frequency tail, the other flanks the tip/high frequency slope regions of the latency tuning curve (TC). By contrast, amplitude enhancement typically does not flank the high frequency slop...

    journal_title:Hearing research

    pub_type: 杂志文章

    doi:10.1016/0378-5955(92)90078-2

    authors: Price JM,Henry KR

    更新日期:1992-11-01 00:00:00

  • Simulation of ILD sensitive neurons in the inferior colliculus of the barn owl.

    abstract::The barn owl (Tyto alba) uses interaural level difference (ILD) as a cue for the localization of sound. The first site of binaural convergence in the pathway that processes ILD is the ventral lateral lemniscus pars posterior (VLVp). Neurons in VLVp receive excitatory input from the contralateral nucleus angularis, and...

    journal_title:Hearing research

    pub_type: 杂志文章

    doi:10.1016/s0378-5955(98)00102-6

    authors: Peddicord R

    更新日期:1998-09-01 00:00:00

  • Ultrastructure and immunohistochemical identification of the extracellular matrix of the chinchilla cochlea.

    abstract::The molecular composition and three-dimensional organization of the extracellular matrix (ECM) was studied by immunofluorescent microscopy, transmission and scanning electron microscopy in three connective tissue structures of the cochlea: the spiral limbus, basilar membrane and spiral ligament. Type II collagen, fibr...

    journal_title:Hearing research

    pub_type: 杂志文章

    doi:10.1016/s0378-5955(98)00219-6

    authors: Tsuprun V,Santi P

    更新日期:1999-03-01 00:00:00

  • The effect of BAPTA and 4AP in scala media on transduction and cochlear gain.

    abstract::We have injected by iontophoresis 4-amino-pyridine, a K+ channel blocker and BAPTA, (a Ca++ chelator), into scala media of the first three turns of the guinea pig cochlea. We measured the reduction in outer hair cell (OHC) receptor current, as indicated by cochlear microphonic measured in scala media evoked by a 207 H...

    journal_title:Hearing research

    pub_type: 杂志文章

    doi:10.1016/j.heares.2005.05.016

    authors: Sellick PM,Robertson D,Patuzzi R

    更新日期:2006-01-01 00:00:00

  • Coordinated expression and function of neurotrophins and their receptors in the rat inner ear during target innervation.

    abstract::We show that trkB and trkC mRNAs, encoding the high-affinity receptor tyrosine kinases for brain-derived neurotrophic factor (BDNF) and neurotrophin-3 (NT-3), respectively, as well as low-affinity nerve growth factor receptor (p75LNGFR) mRNA are expressed in the cochleovestibular ganglion (CVG) before and during inner...

    journal_title:Hearing research

    pub_type: 杂志文章

    doi:10.1016/0378-5955(94)90064-7

    authors: Pirvola U,Arumäe U,Moshnyakov M,Palgi J,Saarma M,Ylikoski J

    更新日期:1994-05-01 00:00:00

  • Spiral ganglion cell site of excitation II: numerical model analysis.

    abstract::An anatomically based model of cochlear neuron electrophysiology has been developed and used to interpret the physiological responses of the auditory neuron to electrical summation and refractory pulse-pair stimuli. For summation pulses, the summation time constant, tau(sum), indicates the ability of the membrane to h...

    journal_title:Hearing research

    pub_type: 杂志文章

    doi:10.1016/j.heares.2006.02.011

    authors: Cartee LA

    更新日期:2006-05-01 00:00:00

  • Sex differences in subcortical auditory processing only partially explain higher prevalence of language disorders in males.

    abstract::Males and females differ in their subcortical evoked responses to sound. For many evoked response measures, the sex difference is driven by a faster developmental decline of auditory processing in males. Using the frequency-following response (FFR), an evoked potential that reflects predominately midbrain processing o...

    journal_title:Hearing research

    pub_type: 杂志文章

    doi:10.1016/j.heares.2020.108075

    authors: Krizman J,Bonacina S,Kraus N

    更新日期:2020-12-01 00:00:00

  • Electrophysiological and morphological evaluation of the acute ototoxicity of sodium nitroprusside.

    abstract::Nitric oxide (NO) is a messenger molecule that mediates several physiological functions and pathological processes. Sodium nitroprusside (SNP), a potent vasodilator, when given clinically as an anti-hypertension agent, exerts its function by releasing NO. It was reported recently that SNP causes a loss of auditory ner...

    journal_title:Hearing research

    pub_type: 杂志文章

    doi:10.1016/s0378-5955(96)00076-7

    authors: Kong WJ,Ren T,Nuttall AL

    更新日期:1996-09-15 00:00:00

  • Manipulating cell cycle regulation in the mature cochlea.

    abstract::Sensorineural hearing loss, which is often caused by degeneration of hair cells in the auditory epithelium, is permanent because lost hair cells are not replaced. Several conceptual approaches can be used to place new hair cells in the auditory epithelium. One possibility is to enhance proliferation of non-sensory cel...

    journal_title:Hearing research

    pub_type: 杂志文章

    doi:10.1016/j.heares.2007.06.005

    authors: Minoda R,Izumikawa M,Kawamoto K,Zhang H,Raphael Y

    更新日期:2007-10-01 00:00:00

  • Roles of inhibition for transforming binaural properties in the brainstem auditory system.

    abstract::This review is concerned with the operation of circuits in the central auditory system, how they transform response features and what functional significance may be attributed to those transformations. We focus on the role that GABAergic inhibition plays in processing interaural intensity disparities (IIDs), the princ...

    journal_title:Hearing research

    pub_type: 杂志文章,评审

    doi:10.1016/s0378-5955(02)00362-3

    authors: Pollak GD,Burger RM,Park TJ,Klug A,Bauer EE

    更新日期:2002-06-01 00:00:00

  • Comparison between the tuning properties of inner hair cells and basilar membrane motion.

    abstract::Measurements were made of inner hair cell receptor potentials and basilar membrane motion in the 17-21 kHz region of the guinea pig cochlea. The latter were made using the Mossbauer technique. Isoamplitude curves at 0.9 mV d.c. receptor potential were compared with isovelocity curves at 0.04 mm/s and the corresponding...

    journal_title:Hearing research

    pub_type: 杂志文章

    doi:10.1016/0378-5955(83)90019-9

    authors: Sellick PM,Patuzzi R,Johnstone BM

    更新日期:1983-04-01 00:00:00

  • Evidence of pitch processing in the N100m component of the auditory evoked field.

    abstract::The latency of the N100m component of the auditory evoked field (AEF) is sensitive to the period and spectrum of a sound. However, little attention was paid so far to the wave shape at stimulus onset, which might have biased previous results. This problem was fixed in the present study by aligning the first major peak...

    journal_title:Hearing research

    pub_type: 杂志文章

    doi:10.1016/j.heares.2006.01.003

    authors: Seither-Preisler A,Patterson R,Krumbholz K,Seither S,Lütkenhöner B

    更新日期:2006-03-01 00:00:00

  • Morphology of the endolymphatic sac in the guinea pig after an acute endolymphatic hydrops.

    abstract::The role of the endolymphatic sac (ES) in endolymph volume homeostasis is speculative. The present study investigates changes of the ES's epithelia and luminal filling after induction of an acute endolymphatic hydrops. After microinjection of 1.1 mul artificial endolymph into scala media of the cochlea, guinea pigs we...

    journal_title:Hearing research

    pub_type: 杂志文章

    doi:10.1016/j.heares.2004.10.010

    authors: Valk WL,Wit HP,Segenhout JM,Dijk F,van der Want JJ,Albers FW

    更新日期:2005-04-01 00:00:00

  • Automatic monitoring of mechano-electrical transduction in the guinea pig cochlea.

    abstract::We have estimated the transfer curve relating instantaneous sound pressure in the ear canal to instantaneous receptor current through the outer hair cells (OHCs) in the basal turn of the guinea pig cochlea using the cochlear microphonic (CM) elicited by continuous 200 Hz tones. The transfer curve is well approximated ...

    journal_title:Hearing research

    pub_type: 杂志文章

    doi:10.1016/s0378-5955(98)00125-7

    authors: Patuzzi R,Moleirinho A

    更新日期:1998-11-01 00:00:00

  • Perilymphatic application of alpha-melanocyte stimulating hormone ameliorates hearing loss caused by systemic administration of cisplatin.

    abstract::It has previously been demonstrated that ototoxicity induced by systemic administration of cisplatin is reduced by concomitant systemic administration of alpha-melanocyte stimulating hormone (alpha-MSH). In this study we investigated the effects of cochlear, perilymphatic application of alpha-MSH during intraperitonea...

    journal_title:Hearing research

    pub_type: 杂志文章

    doi:10.1016/S0378-5955(03)00396-4

    authors: Wolters FL,Klis SF,Hamers FP,de Groot JC,Smoorenburg GF

    更新日期:2004-03-01 00:00:00