Abstract:
BACKGROUND & AIMS:Ethanol causes both tolerance and sensitization of Kupffer cells. This study was designed to evaluate temporal effects of ethanol in an attempt to understand this paradox. METHODS:Rats were given ethanol (4 g/kg body wt) intragastrically, and Kupffer cells were isolated 0-48 hours later. After addition of lipopolysaccharide (LPS), intracellular calcium concentration ([Ca2+]i) was measured using a microspectrofluorometer with the fluorescent indicator fura-2, and tumor necrosis factor alpha (TNF-alpha) was measured by enzyme-linked immunosorbent assay. CD14 was evaluated by Western and Northern analysis. RESULTS:Two hours after ethanol administration, the LPS-induced increase in [Ca2+]i and TNF-alpha release by Kupffer cells was diminished by 50%, and these parameters were reciprocally enhanced twofold at 24 hours. Sterilization of the gut with antibiotics blocked all effects of ethanol on [Ca2+]i and TNF-alpha release completely. Twenty-four hours after ethanol, CD14 in Kupffer cells was elevated about fivefold. CONCLUSIONS:Kupffer cells isolated from rats early after ethanol exhibited tolerance to LPS, whereas sensitization was observed later. It is likely that both of these phenomena are caused by gut-derived endotoxin and that sensitization in Kupffer cells is caused by increases in CD14.
journal_name
Gastroenterologyjournal_title
Gastroenterologyauthors
Enomoto N,Ikejima K,Bradford B,Rivera C,Kono H,Brenner DA,Thurman RGdoi
10.1016/s0016-5085(98)70211-2subject
Has Abstractpub_date
1998-08-01 00:00:00pages
443-51issue
2eissn
0016-5085issn
1528-0012pii
S0016508598001723journal_volume
115pub_type
杂志文章相关文献
GASTROENTEROLOGY文献大全abstract:BACKGROUND AND AIMS:Autoimmune responses against colonic epithelium may play a role in the development of colonic inflammation associated with ulcerative colitis (UC). In this study, we established and characterized B-cell lines and clones that produced anticolon antibody from inflamed colonic mucosa of UC subjects. M...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/gast.2001.25477
更新日期:2001-07-01 00:00:00
abstract::An in vitro fecal incubation system was used to demonstrate how lactulose influences ammonia metabolism in the colon. Lactulose and other fermentable substrates (glucose, mannitol, and sorbitol), pH and organic acid were varied independently so that their different effects could be determined. Fermentable substrate ca...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:
更新日期:1978-03-01 00:00:00
abstract::In sickle cell anemia the shortened survival of red blood cells presents the liver with an augmented load of bilirubin for hepatic clearance. To determine the effects of this excessive bilirubin load on the microsomal conjugating enzyme, hepatic bilirubin UDP-glucuronyltransferase, levels of this enzyme were measured ...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:
更新日期:1978-02-01 00:00:00
abstract::Motor abnormalities of the small bowel that occur only during the waking state have been reported in the irritable bowel syndrome (IBS), suggesting that central nervous system arousal is a necessary condition for expression of the disorder and that it may reflect inappropriate brain-gut interaction. This possible rela...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1016/0016-5085(92)91089-m
更新日期:1992-07-01 00:00:00
abstract:BACKGROUND & AIMS:The pregnane X receptor (PXR) regulates an array of genes involved in the response to xenobiotics. Evidence from several studies suggests that xenobiotic metabolism may play a role in inflammatory bowel disease (IBD) and that low levels of PXR may be associated with disease expression. The aim of this...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/j.gastro.2005.12.008
更新日期:2006-02-01 00:00:00
abstract::A patient with gastric outlet syndrome (Bouveret's syndrome) caused by a large gallstone impacted in the duodenal bulb was successfully treated by extracorporeal shock-wave lithotripsy. Thus, open abdominal surgery could be avoided. For disintegration of the stone, three consecutive lithotripsy procedures were necessa...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1016/0016-5085(89)90086-3
更新日期:1989-08-01 00:00:00
abstract::We tested methyl tertiary butyl ether both in vitro and in vivo to evaluate its efficacy as a potential cholesterol gallstone solvent for direct instillation into the human gallbladder or bile duct. Like diethyl ether, methyl tertiary butyl ether is an aliphatic ether with an excellent cholesterol-solubilizing capacit...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1016/s0016-5085(85)80143-8
更新日期:1985-01-01 00:00:00
abstract::Three cases of Stevens-Johnson syndrome with intestinal involvement are described. Two patients had esophageal involvement, the severity of which paralleled skin lesions and, in 1 case, probably contributed to death. Dysphagia and bleeding were manifestations. The third patient was unique and had gastric, small and la...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1016/0016-5085(86)90585-8
更新日期:1986-08-01 00:00:00
abstract:BACKGROUND & AIMS:Interstitial cells of Cajal (ICC) serve as pacemaker cells and mediators of neurotransmission from the enteric nervous system to gastrointestinal muscles. ICC develop from mesenchymal cells that express c-Kit, and signaling via Kit receptors is necessary for normal development of ICC. We studied the f...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1016/s0016-5085(99)70560-3
更新日期:1999-07-01 00:00:00
abstract::Liver copper concentration was studied in four groups of age-matched patients and in 9 patients with Wilson's disease of comparable age. Neutron activation analysis of hepatic copper concentration revealed markedly elevated hepatic copper levels, comparable with those in Wilson's disease, in 13 of 15 patients (P less ...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:
更新日期:1978-11-01 00:00:00
abstract:BACKGROUND & AIMS:Interactions between genetic and environmental factors are believed to be involved in onset and initiation of inflammatory bowel disease. We analyzed the interaction between gastrointestinal mucosal microbiota and host genes in twin pairs discordant for ulcerative colitis (UC) to study the functional ...
journal_title:Gastroenterology
pub_type: 杂志文章,多中心研究
doi:10.1053/j.gastro.2011.04.011
更新日期:2011-07-01 00:00:00
abstract:BACKGROUND & AIMS:Patients with previous stomach and terminal ileum resections are often treated with intramuscular vitamin B12 injections. Disadvantages are, on a worldwide scale, the frequent need for medical personnel to administer injections and the sometimes painful way of application. This study was designed to i...
journal_title:Gastroenterology
pub_type: 临床试验,杂志文章
doi:10.1053/gast.1997.v113.pm9247460
更新日期:1997-08-01 00:00:00
abstract::Microsatellite instability (MSI) caused by mismatch repair deficiency (dMMR) is detected in a small proportion of pancreatic ductal adenocarcinomas (PDACs). dMMR and MSI have been associated with responses of metastatic tumors, including PDACs, to immune checkpoint inhibitor therapy. We performed immunohistochemical a...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/j.gastro.2017.11.009
更新日期:2018-03-01 00:00:00
abstract::The influence of endogenous prostaglandins on secretin-mediated inhibition of gastric acid secretion was examined in 6 mongrel dogs with Thomas gastric and Herrera pancreatic cannulas. The dogs were given intravenous pentagastrin (1 microgram/kg.h) during the 180-min experiment, and graded doses of secretin (0.3-1.5 m...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1016/s0016-5085(88)80007-6
更新日期:1988-09-01 00:00:00
abstract:BACKGROUND & AIMS:Several studies have reported that low doses of interferon can delay the development of hepatocellular carcinoma (HCC) and progression of chronic hepatitis C. We investigated the incidence of clinical events among participants of the Evaluation of PegIntron in Control of Hepatitis C Cirrhosis (EPIC)3 ...
journal_title:Gastroenterology
pub_type: 杂志文章,多中心研究,随机对照试验
doi:10.1053/j.gastro.2011.03.010
更新日期:2011-06-01 00:00:00
abstract:BACKGROUND & AIMS:This study investigated whether obesity, diabetes, and other metabolic factors are independently associated with hepatocellular carcinoma (HCC), stratified by hepatitis B virus (HBV) and hepatitis C virus (HCV) serostatus, and explored the possible joint influence of obesity/diabetes and HBV/HCV infec...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/j.gastro.2008.03.073
更新日期:2008-07-01 00:00:00
abstract::In dispersed acini prepared from guinea pig pancreas, ethanol inhibited the increase in amylase secretion caused by cholecystokinin, carbachol, secretin, or vasoactive intestinal peptide. Ethanol did not alter binding of [125I] vasoactive intestinal peptide to pancreatic acinar cells or the inhibition of binding cause...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:
更新日期:1979-05-01 00:00:00
abstract:BACKGROUND & AIMS:A previous 4-week trial of telbivudine in patients with chronic hepatitis B indicated marked antiviral effects with good tolerability, leading to the present 1-year phase 2b trial. METHODS:This randomized, double-blind, multicenter trial evaluated the efficacy and safety of telbivudine 400 or 600 mg/...
journal_title:Gastroenterology
pub_type: 临床试验,杂志文章,多中心研究,随机对照试验
doi:10.1016/j.gastro.2005.05.053
更新日期:2005-08-01 00:00:00
abstract::To determine the long-term efficacy, safety, and toxicity of omeprazole, we studied 40 patients with Zollinger-Ellison syndrome given omeprazole for 6-51 mo (median 29). The mean daily dose of omeprazole required to control gastric acid secretion was 82 +/- 31 mg. Thirty-one patients required omeprazole once per day. ...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1016/0016-5085(89)91485-6
更新日期:1989-10-01 00:00:00
abstract::A numerically important group of patients with functional gastrointestinal disorders have chronic symptoms that can be attributed to the gastroduodenal region. Based on the consensus opinion of an international panel of clinical investigators who reviewed the available evidence, a classification of the functional gast...
journal_title:Gastroenterology
pub_type: 杂志文章,评审
doi:10.1053/j.gastro.2005.11.059
更新日期:2006-04-01 00:00:00
abstract:BACKGROUND & AIMS:Dilutional hyponatremia is a frequent complication of cirrhosis partly because of nonosmotic vasopressin release. No effective therapy exists for this complication. Therefore, we investigated the effects of VPA-985, an orally active vasopressin V2 receptor antagonist, in patients with cirrhosis and di...
journal_title:Gastroenterology
pub_type: 临床试验,杂志文章,多中心研究,随机对照试验
doi:10.1053/gast.2003.50143
更新日期:2003-04-01 00:00:00
abstract:BACKGROUND:Although it has recently become possible to detect hepatitis C viral antigens in liver biopsy specimens, the frequency and clinical significance of this finding remains uncertain. Therefore, 49 liver biopsy specimens from 35 patients with hepatitis C were studied to make these assessments. METHODS:Hepatitis...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1016/0016-5085(93)90905-r
更新日期:1993-09-01 00:00:00
abstract:BACKGROUND & AIMS:Previous research has yielded conflicting data as to whether the natural history of inflammatory bowel disease follows a seasonal pattern. The purpose of this study was to determine whether relapse of inflammatory bowel disease follows a seasonal pattern either across a cohort of patients or within in...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/j.gastro.2003.12.003
更新日期:2004-03-01 00:00:00
abstract:BACKGROUND & AIMS:Viral genotypes have been associated with different severity and outcome of hepatitis C virus (HCV)-related liver disease. The aim of this study was to determine whether HCV genotypes may influence the cirrhosis-related risk of the development of hepatocellular carcinoma (HCC). METHODS:Three groups o...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/gast.1996.v111.pm8698200
更新日期:1996-07-01 00:00:00
abstract:BACKGROUND/AIMS:The diagnosis of cholangiocarcinoma in primary sclerosing cholangitis (PSC), even with the use of current imaging techniques and brush cytology, is difficult and particularly important in patients being assessed for liver transplantation. This study investigated the accuracy of serum levels of a combina...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1016/0016-5085(95)90462-x
更新日期:1995-03-01 00:00:00
abstract:BACKGROUND & AIMS:The effects of leukotriene (LT) D(4) on intestinal epithelial cells govern events that are involved in cell survival and colon cancer, notably increased expression of cyclooxygenase (COX)-2 and enhanced production of prostaglandin E(2). We investigated possible correlations between distribution of the...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/gast.2003.50011
更新日期:2003-01-01 00:00:00
abstract:BACKGROUND & AIMS:Narcotic bowel syndrome (NBS) is a subset of opioid bowel dysfunctions that results from prolonged treatment with narcotics and is characterized by chronic abdominal pain. NBS is under-recognized and its molecular mechanisms are unknown. We aimed to (1) develop a rat model of NBS and (2) to investigat...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/j.gastro.2010.03.046
更新日期:2010-08-01 00:00:00
abstract:BACKGROUND:The clonality of hepatocellular carcinoma is still controversial. To evaluate the clonality of this cancer, mostly small multiple hepatocellular carcinomas of 18 patients were analyzed by DNA fingerprinting. METHODS:DNA from the liver tissues was digested with HinfI. After gel electrophoresis, in gel-hybrid...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1016/0016-5085(93)90153-4
更新日期:1993-11-01 00:00:00
abstract:BACKGROUND & AIMS:Postprandial glycemia excursions increase after gastric bypass surgery; this effect is even greater among patients with recurrent hypoglycemia. These patients also have increased postprandial levels of insulin and glucagon-like peptide 1 (GLP-1). We performed a clinical trial to determine the role of ...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/j.gastro.2013.11.044
更新日期:2014-03-01 00:00:00
abstract::In vitro neurite outgrowth and neuronal survival are promoted by laminin, and neuronal migration is promoted by collagen type IV. This led to the hypothesis that Hirschsprung's disease (HD) could be the result of an abnormal extracellular matrix microenvironment in the affected bowel during embryogenesis. Using indire...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:
更新日期:1992-04-01 00:00:00