Abstract:
:The sequence of RNA genome segment S4 of the avian reovirus (ARV) strain S1133 was determined. S4 RNA is 1185 base pairs long and contains one open reading frame encoding a protein of 367 amino acid residues (40.6 kDa), the similar size as the known S4 gene product (sigma NS), with a net charge of -1 at neutral pH. The S4 RNA sequence possesses a pentanucleotide sequence UCAUC at the 3'-terminus of its plus strand like in ARV S1 and S3 segments and ten segments of mammalian reovirus (MRV). The predicted amino acid sequence comparison revealed that the homology is 44.02%, 45.71%, and 42.33% for ARV sigma NS and three serotypes of MRV sigma NS, respectively. The relatively high content of alpha-helix structure in the C-terminal portion of ARV sigma NS suggests that this protein may functionally relate to MRV sigma NS. Northern blot hybridization showed that a 32P-labeled cDNA insert S4-49 from ARV S4 RNA cross-hybridized with the corresponding RNA segments of all seven strains of ARV tested.
journal_name
Arch Viroljournal_title
Archives of virologyauthors
Chiu CJ,Lee LHdoi
10.1007/s007050050258subject
Has Abstractpub_date
1997-01-01 00:00:00pages
2515-20issue
12eissn
0304-8608issn
1432-8798journal_volume
142pub_type
杂志文章abstract::Vanilla necrosis potyvirus (VNV) is the cause of significant losses to the South Pacific islands vanilla crop. The gene for the coat protein of VNV has been cloned and sequenced. Comparison of this gene with other potyviral coat protein sequences revealed 97% nucleotide sequence homology (98% amino acid homology) to a...
journal_title:Archives of virology
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journal_title:Archives of virology
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journal_title:Archives of virology
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journal_title:Archives of virology
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journal_title:Archives of virology
pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:1986-01-01 00:00:00
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更新日期:2011-11-01 00:00:00
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journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01315679
更新日期:1978-01-01 00:00:00
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pub_type: 杂志文章
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更新日期:2013-06-01 00:00:00
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pub_type: 杂志文章
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journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01348017
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journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s007050050090
更新日期:1997-01-01 00:00:00
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journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01379138
更新日期:1994-01-01 00:00:00
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journal_title:Archives of virology
pub_type: 杂志文章
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更新日期:2002-11-01 00:00:00
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journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01314964
更新日期:1995-01-01 00:00:00
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更新日期:2002-08-01 00:00:00
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更新日期:2016-09-01 00:00:00
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pub_type: 杂志文章
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abstract::Porcine reproductive and respiratory syndrome (PRRS) is one of the most highly infectious diseases in the pig industry, resulting in enormous economic losses worldwide. In this study, a PRRS virus (PRRSV) strain was isolated from primary porcine alveolar macrophage cells in Xinjiang in northwest China. This new strain...
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