Cloning and nucleotide sequencing of the S4 genome segment of avian reovirus S1133.

Abstract:

:The sequence of RNA genome segment S4 of the avian reovirus (ARV) strain S1133 was determined. S4 RNA is 1185 base pairs long and contains one open reading frame encoding a protein of 367 amino acid residues (40.6 kDa), the similar size as the known S4 gene product (sigma NS), with a net charge of -1 at neutral pH. The S4 RNA sequence possesses a pentanucleotide sequence UCAUC at the 3'-terminus of its plus strand like in ARV S1 and S3 segments and ten segments of mammalian reovirus (MRV). The predicted amino acid sequence comparison revealed that the homology is 44.02%, 45.71%, and 42.33% for ARV sigma NS and three serotypes of MRV sigma NS, respectively. The relatively high content of alpha-helix structure in the C-terminal portion of ARV sigma NS suggests that this protein may functionally relate to MRV sigma NS. Northern blot hybridization showed that a 32P-labeled cDNA insert S4-49 from ARV S4 RNA cross-hybridized with the corresponding RNA segments of all seven strains of ARV tested.

journal_name

Arch Virol

journal_title

Archives of virology

authors

Chiu CJ,Lee LH

doi

10.1007/s007050050258

subject

Has Abstract

pub_date

1997-01-01 00:00:00

pages

2515-20

issue

12

eissn

0304-8608

issn

1432-8798

journal_volume

142

pub_type

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