Abstract:
:Homologous restriction factor 20 (HRF20, CD59) is one of the complement regulatory factors. In this study, the complement-resistant retroviral vector, which possesses the HRF20 gene as a selection gene, was constructed and examined. The virus-producing cell, transduced with complement-resistant retroviral vector, was established after complement-dependent selection. NIH3T3 and PK15 cells transduced with this virus-producing cell were successfully selected by complement-dependent selection, which showed significant expression of the HRF20 antigen. In addition, these cells, transduced with tissue plasminogen activator (tPA) cDNA using complement-resistant retroviral vector, expressed tPA antigen after complement-dependent selection. These findings suggest that complement-resistant retroviral vector can be used for the double transduction of HRF20, as well as other genes.
journal_name
Gene Therjournal_title
Gene therapyauthors
Hayashi S,Emi N,Okada H,Nagasaka T,Yokoyama I,Takagi Hdoi
10.1038/sj.gt.3300574subject
Has Abstractpub_date
1998-02-01 00:00:00pages
282-5issue
2eissn
0969-7128issn
1476-5462journal_volume
5pub_type
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