Abstract：
:This study was based on the hypothesis that both primary sequence and methylation status of the GTPase activating protein (GAP) gene limits expression of p100 GAP to primate placenta. Due to alternate splicing, a 65-bp insert appears between the first and second coding exons of p100 GAP mRNA, and translation of p100 GAP initiates within this insert. Examination of the sequence surrounding the 65-bp insert revealed that the monkey GAP gene contained both the 3' splice donor site and the internal start codon, whereas the mouse GAP gene contained neither. To address p100 GAP tissue specificity, the methylation status of the GAP gene was examined. Site-specific demethylation was found to correlate with synthesis of p100 GAP, suggesting that methylation regulates the expression of different GAP isoforms. The results of this study provide a mechanistic basis for the observation that p100 GAP is synthesized only in primate placenta and suggest that its expression is regulated, in part, by gene methylation.
journal_name
Placentajournal_title
Placentaauthors
Liao L,Trouba KJ,Vorce RLdoi
10.1016/s0143-4004(98)90012-1subject
Has Abstractpub_date
1998-03-01 00:00:00pages
225-30issue
2-3eissn
0143-4004issn
1532-3102pii
S0143-4004(98)90012-1journal_volume
19pub_type
杂志文章相关文献
PLACENTA文献大全abstract：:Pregnancy-specific beta 1-glycoprotein (Schwangerschaftsprotein, SP1) is elevated in the amniotic fluid in Meckel's syndrome. In an attempt to clarify this phenomenon, we studied fetal tissues by immunohistochemistry for the distribution of SP1 in normal fetuses and fetuses with Meckel's syndrome. The amount of SP1 wa...
journal_title：Placenta
pub_type： 杂志文章
doi：10.1016/0143-4004(87)90070-1
更新日期：1987-07-01 00:00:00
abstract：:Maternal infection of Trypanosoma cruzi is associated with premature births, abortions and placentitis. A decrease in EGF levels has been suggested to occur in animals infected by T. cruzi, but there is no research about the levels of EGF in human patients with Chagas' disease. We evaluated serum EGF levels in pregnan...
journal_title：Placenta
pub_type： 杂志文章
doi：10.1016/j.placenta.2003.09.008
更新日期：2004-04-01 00:00:00
abstract：:Previous clinical studies have shown the efficacy of a two-stage surgical procedure - the induced membrane (IM) technique - for reconstruction of large bone defects or bone non-union. The first stage involves radical debridement and insertion of a cement spacer into the bone defect. The second stage, performed weeks t...
journal_title：Placenta
pub_type： 杂志文章,评审
doi：10.1016/j.placenta.2017.06.340
更新日期：2017-11-01 00:00:00
abstract：UNLABELLED:Betamethasone (BET) is a widely used treatment for women who are at high risk of preterm delivery. In sheep, BET-induced growth restriction was found to be associated with reduced placenta lactogen (PL), a key regulator of fetal growth. We therefore hypothesized that also in humans a single course of BET adm...
journal_title：Placenta
pub_type： 杂志文章
doi：10.1016/j.placenta.2013.02.002
更新日期：2013-05-01 00:00:00
abstract：:Bidirectional transplacental exchange characterizes human pregnancy. Cells exchanged between mother and fetus can durably persist as microchimerism and may have both short- and long-term consequences for the recipient. The amount, type, and persistence of microchimerism are influenced by obstetric characteristics, pre...
journal_title：Placenta
pub_type： 杂志文章,评审
doi：10.1016/j.placenta.2017.08.071
更新日期：2017-12-01 00:00:00
abstract：:Cytosol preparations of human term placenta in Tris-HCl buffer containing dithiothreitol were incubated with [3H]dexamethasone at 4 degrees C. Under these conditions the specific binding of [3H]dexamethasone was low when compared with cytosol preparations of sheep fetal lung or ovine placenta. The inclusion of sodium ...
journal_title：Placenta
pub_type： 杂志文章
doi：10.1016/s0143-4004(84)80054-5
更新日期：1984-03-01 00:00:00
abstract：:We tested the hypothesis that the expression of placental connective tissue growth factor (CTGF) is enhanced in pregnancies complicated by severe preeclampsia (PE) or fetal growth restriction (FGR). CTGF expression was analyzed using immunostaining, western blot and real-time quantitative PCR in placental samples obta...
journal_title：Placenta
pub_type： 杂志文章
doi：10.1016/j.placenta.2009.08.006
更新日期：2009-11-01 00:00:00
abstract：INTRODUCTION:Preeclampsia is a severe pregnancy complication mostly due to inadequate vascular dilation and remodeling of spiral arteries. VEGF, the major factor for angiogenesis, is necessary for modulating angiogenic processes in the placenta. Hence reduction of VEGF in gestational hypertension may also lead to hypop...
journal_title：Placenta
pub_type： 杂志文章
doi：10.1016/j.placenta.2016.08.088
更新日期：2016-10-01 00:00:00
abstract：INTRODUCTION:Type 1 diabetes (T1D) is associated with adverse pregnancy outcome, usually attributed to hyperglycemia. Recently, we showed that pregnancy outcome in normoglycemic T1D rats was characterized by decreased fetal and placental weight, suggesting impaired placental development. In the present study, we tested...
journal_title：Placenta
pub_type： 杂志文章
doi：10.1016/j.placenta.2014.12.004
更新日期：2015-02-01 00:00:00
abstract：:Placentas and plasma from women with and without Chagas' disease and cultures of human placental villi with Trypanosoma cruzi, neuraminidase, phospholipase A2 and phospholipase C were analyzed in order to verify if the alterations in placental alkaline phosphatase (PLAP) enzyme activity are caused by T. cruzi as obser...
journal_title：Placenta
pub_type： 杂志文章
doi：10.1016/j.placenta.2004.08.014
更新日期：2005-11-01 00:00:00
abstract：:The transport of large amounts of Ca2+ by the plasma membranes of human placental syncytiotrophoblast is essential to the mineralization of the growing fetal skeleton. We have investigated transport by the basal (fetal-facing) plasma membrane (BPM). Ca2+ was taken up by purified BPM vesicles in a time-dependent manner...
journal_title：Placenta
pub_type： 杂志文章
doi：10.1016/s0143-4004(05)80451-5
更新日期：1994-02-01 00:00:00
abstract：:The aim of this study was to investigate localization and content of activin beta A-subunit and activin receptors in gestational tissues throughout pregnancy and in association with term labour. Placenta and fetal membranes were collected from women undergoing first and second trimester terminations and from women bef...
journal_title：Placenta
pub_type： 杂志文章
doi：10.1053/plac.2002.0787
更新日期：2002-04-01 00:00:00
abstract：:Second trimester maternal serum human chorionic gonadotrophin (hCG) levels in women who remained normotensive but delivered an unexplained growth retarded infant were compared with those from a control group and a group of women who developed pre-eclampsia in a retrospective observational study. Our hypothesis was tha...
journal_title：Placenta
pub_type： 杂志文章
doi：10.1016/s0143-4004(98)90002-9
更新日期：1998-03-01 00:00:00
abstract：:Intrauterine infection has been associated with fetal brain injury and preterm birth. We have recently shown that repeated exposure to bacterial endotoxin leads to hypoxia and brain injury in the preterm ovine fetus and we considered it possible that endotoxin could also damage the placenta. Our aim therefore was to a...
journal_title：Placenta
pub_type： 杂志文章
doi：10.1016/s0143-4004(03)00104-8
更新日期：2003-08-01 00:00:00
abstract：:Marijuana is one of the most abused drugs among pregnant women leading to maternal and fetal abnormalities. Cannabinoids are the active ingredients of marijuana, which interact with cannabinoid receptors such as CNR1 and CNR2 to activate cellular signaling pathways. Human endometrium and placenta are known to express ...
journal_title：Placenta
pub_type： 杂志文章
doi：10.1016/j.placenta.2018.05.002
更新日期：2018-06-01 00:00:00
abstract：:Fetal growth restriction (FGR) is the failure of a fetus to reach its full genetic growth potential. It occurs in up to 8% of pregnancies, and after premature birth is the second leading cause of infant mortality and morbidity. There is no treatment currently available for FGR. Its primary cause, when not attributable...
journal_title：Placenta
pub_type： 杂志文章
doi：10.1016/j.placenta.2015.03.003
更新日期：2015-06-01 00:00:00
abstract：:The establishment of an appropriate fetomaternal vessel system is a prerequisite for prevention of pregnancy associated pathologies. Notch receptors and ligands are manifoldly involved in vascular development and angiogenesis. To further characterize the process of human placental vasculo- and angiogenesis we investig...
journal_title：Placenta
pub_type： 杂志文章
doi：10.1016/j.placenta.2011.04.018
更新日期：2011-08-01 00:00:00
abstract：:Xanthine dehydrogenase/oxidase (XDH, EC 1.1.1.204, XO, EC 1.2.3.2) produces uric acid, and in the oxidase form also generates the free radical superoxide. Previous reports failed to demonstrate XDH/XO activity in human placenta. Our objective was to determine evidence of XDH/XO in human placenta. We developed a cDNA p...
journal_title：Placenta
pub_type： 杂志文章
doi：10.1016/s0143-4004(96)90061-2
更新日期：1996-07-01 00:00:00
abstract：:Heme oxygenase (HO) is the rate-limiting enzyme in the heme catabolic pathway and highly expressed in the placenta. Deficiencies in HO-1, the inducible isoform, have been associated with pregnancy disorders, such as recurrent miscarriages, intrauterine growth retardation, and pre-eclampsia. The aim of this study was t...
journal_title：Placenta
pub_type： 杂志文章
doi：10.1016/j.placenta.2009.07.012
更新日期：2009-10-01 00:00:00
abstract：:There is accumulating evidence that deficient trophoblast invasion of the placental bed spiral arteries is crucial to the pathogenesis of pre-eclampsia and intrauterine growth restriction. However, the factors which regulate the process of trophoblast invasion remain unclear. We have investigated whether extravillous ...
journal_title：Placenta
pub_type： 杂志文章
doi：10.1053/plac.1999.0427
更新日期：1999-11-01 00:00:00
abstract：:Placental villi of early and term human placentae were dissociated with trypsin and mixed cell cultures were established. The different cell types were identified and estimated over a 14-day culture period using antibodies to keratin and vimentin filaments and their capacity to phagocytose yeast. The three main cell t...
journal_title：Placenta
pub_type： 杂志文章
doi：10.1016/s0143-4004(84)80047-8
更新日期：1984-01-01 00:00:00
abstract：OBJECTIVE:To evaluate pregnancy outcome and ultrasound diagnosis in patients with histologically-confirmed placental chorioangioma. STUDY DESIGN:Placentas with histological diagnosis of chorioangioma were identified and medical charts were reviewed for findings of ultrasound examinations. Pregnancy outcomes were compa...
journal_title：Placenta
pub_type： 杂志文章
doi：10.1016/j.placenta.2011.06.007
更新日期：2011-09-01 00:00:00
abstract：:Placentae from both smoking and non-smoking mothers were studied with respect to P450-dependent xenobiotic and steroid-metabolizing reactions, GSHt activity with different substrates and umbilical blood cotinine levels. Catalytic activities were determined in both freshly prepared homogenate subfractions and subfracti...
journal_title：Placenta
pub_type： 杂志文章
doi：10.1016/s0143-4004(05)80445-x
更新日期：1990-01-01 00:00:00
abstract：INTRODUCTION:Visualization of the retroplacental clear space (RPCS) may provide critical insight into the development of abnormally invasive placenta (AIP). In this pre-clinical study, we characterized the appearance of the RPCS on magnetic resonance imaging (MRI) during the second half of gestation using a liposomal g...
journal_title：Placenta
pub_type： 杂志文章
doi：10.1016/j.placenta.2019.01.017
更新日期：2019-02-01 00:00:00
abstract：:The human placenta contains two types of 11beta-hydroxysteroid dehydrogenase (11beta-HSD). The exclusive oxidase 11beta-HSD2 has been suggested to protect the fetus from high levels of maternal glucocorticoids by converting cortisol to inactive cortisone. Perfused term human placenta was used to examine the activity o...
journal_title：Placenta
pub_type： 杂志文章
doi：10.1053/plac.1998.0352
更新日期：1999-01-01 00:00:00
abstract：INTRODUCTION:During a pregnancy complicated by diabetes, the human placenta undergoes a number of functional and structural pathologic changes, such as increased placental weight and increased incidence of placental lesions including villous maturational defects and fibrinoid necrosis. The pathologic findings reported ...
journal_title：Placenta
pub_type： 杂志文章,评审
doi：10.1016/j.placenta.2014.11.021
更新日期：2015-02-01 00:00:00
abstract：BACKGROUND:The aim of this study was to determine whether DNA-associated micro-particles (MPs) in maternal plasma express fetal-derived human leukocyte antigen-G (HLA-G) or placental alkaline phosphatase (PLAP) and whether the levels differ between women with normotensive pregnancies and preeclampsia. METHODS:DNA-asso...
journal_title：Placenta
pub_type： 杂志文章
doi：10.1016/j.placenta.2009.06.012
更新日期：2009-10-01 00:00:00
abstract：INTRODUCTION:Elevated circulating non-esterified fatty acids including oleic acid (OA) are associated with many pregnancy related complications. Prostaglandins (PGs) play crucial roles during parturition. We investigated the effect of OA supplementation on PG production using an in vitro model of ovine placenta. METHO...
journal_title：Placenta
pub_type： 杂志文章
doi：10.1016/j.placenta.2015.07.128
更新日期：2015-09-01 00:00:00
abstract：INTRODUCTION:Intrahepatic cholestasis of pregnancy (ICP) is a liver disorder unique to pregnancy that is associated with increased rates of fetal distress and demise. While acute hypoxia is believed to cause the pathophysiology of ICP, direct molecular evidence for this is lacking. Here, we analyzed expression of three...
journal_title：Placenta
pub_type： 杂志文章
doi：10.1016/j.placenta.2014.06.372
更新日期：2014-09-01 00:00:00
abstract：BACKGROUND:Alloimmunization against human platelet antigens (HPAs) can occur prenatally and induce fetal/neonatal alloimmune thrombocytopenia (FNAIT). The aim of this study was to identify placental histological features associated with platelet alloimmunization and their clinical significance. METHODS:This study exam...
journal_title：Placenta
pub_type： 杂志文章
doi：10.1016/j.placenta.2016.10.009
更新日期：2016-12-01 00:00:00
