Isolation of liver nuclei that retain functional trans-membrane transport.

Abstract:

:We have developed a method for the rapid isolation of hepatocyte nuclei, which employs gentle homogenization and centrifugation conditions, and involves minimal processing time. The purified nuclei were morphologically unaltered when observed by light and electron microscopy. No significant contamination from cytoplasm or mitochondria was detected when assessed by marker enzymes. Membrane transport function, measured as ATP-dependent calcium uptake, was intact. This isolation method was devised to be applicable to studies that involve measurement of uptake and active transport of a variety of substances by the cell nucleus.

authors

Ho YF,Guenthner TM

doi

10.1016/s1056-8719(97)00082-8

subject

Has Abstract

pub_date

1997-11-01 00:00:00

pages

163-8

issue

3

eissn

1056-8719

issn

1873-488X

pii

S1056-8719(97)00082-8

journal_volume

38

pub_type

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