Abstract：
BACKGROUND & AIMS:Caco-2 cells have been used extensively to elucidate events involved in intestinal cell proliferation and differentiation. Because individual isoforms of protein kinase C (PKC) and p21waf1, a cyclin-dependent kinase inhibitor, may regulate these processes, their role(s) on the growth and differentiation of Caco-2 cells were assessed. METHODS:Protein abundance and subcellular distribution of several PKC isoforms, as well as the expression of p21waf1, were examined in preconfluent and postconfluent cells. RESULTS:In cells at confluence (approximately 7 days postplating) and during their postconfluent phase (up to 20 days postplating), both total protein expression of PKC-alpha and its particulate distribution increased compared with their 3-day postplated counterparts. These findings were in agreement with those obtained by immunocytochemistry of PKC-alpha. In contrast, neither the total expression nor the subcellular distribution of PKC-betaI, -betaII, -delta, or -zeta changed significantly during these time periods. In addition, the expression of p21waf1, which can be induced by PKC-alpha, increased in postconfluent cells. CONCLUSIONS:PKC-alpha, but not other isoforms of PKC, may modulate the proliferation and differentiation of Caco-2 cells. This regulation appears to be mediated, at least in part, via a mechanism involving p21waf1.
journal_name
Gastroenterologyjournal_title
Gastroenterologyauthors
Abraham C,Scaglione-Sewell B,Skarosi SF,Qin W,Bissonnette M,Brasitus TAdoi
10.1016/s0016-5085(98)70533-5subject
Has Abstractpub_date
1998-03-01 00:00:00pages
503-9issue
3eissn
0016-5085issn
1528-0012pii
S0016508598002194journal_volume
114pub_type
杂志文章相关文献
GASTROENTEROLOGY文献大全abstract：BACKGROUND & AIMS:Simeprevir (TMC435) is an oral NS3/4 protease inhibitor in phase III trials for chronic hepatitis C virus (HCV) infection. We performed a phase IIb, randomized, double-blind, placebo-controlled trial to evaluate the efficacy and safety of the combination of simeprevir, peginterferon-α2a (PegIFN), and ...
journal_title：Gastroenterology
pub_type： 杂志文章,多中心研究,随机对照试验
doi：10.1053/j.gastro.2013.10.058
更新日期：2014-02-01 00:00:00
abstract：BACKGROUND & AIMS:Among the human mucin complementary DNAs thus far identified, two (MUC5AC and MUC6) were cloned from stomach libraries. This study examines the distribution of MUC6 in normal tissues and compares it with that of MUC5AC as well as with the expression of Lewis blood group antigens. METHODS:Affinity-pur...
journal_title：Gastroenterology
pub_type： 评论,杂志文章
doi：10.1016/0016-5085(95)90379-8
更新日期：1995-09-01 00:00:00
abstract：BACKGROUND & AIMS:Indomethacin impairs liver microsomal monooxygenase activities mediated by cytochrome P450 (CYP). We investigated the inhibition mechanism and the isoform selectivity in vitro and in vivo. METHODS:In an in vitro study, liver microsomes from male Wistar rats were preincubated with indomethacin and a r...
journal_title：Gastroenterology
pub_type： 杂志文章
doi：10.1053/gast.2002.31886
更新日期：2002-03-01 00:00:00
abstract：:Helicobacter pylori infection has been associated with gastritis, duodenal ulcer, gastric ulcer, and the epidemic form of gastric carcinoma. Eradication of H. pylori infection has proven to be difficult. Recently, combinations of antimicrobial drugs have been shown to eradicate greater than 50% of infections; however,...
journal_title：Gastroenterology
pub_type： 杂志文章
doi：10.1016/0016-5085(92)90095-g
更新日期：1992-02-01 00:00:00
abstract：BACKGROUND & AIMS:Aberrant activation of β-catenin and Yes-associated protein 1 (Yap1) signaling pathways have been associated with the development of multiple tumor types. Yap functions as a transcriptional coactivator by interacting with TEA domain DNA binding proteins. We investigated the interactions among these pa...
journal_title：Gastroenterology
pub_type： 杂志文章,多中心研究
doi：10.1053/j.gastro.2014.05.004
更新日期：2014-09-01 00:00:00
abstract：BACKGROUND & AIMS:Liver graft dysfunction is related to hypothermic preservation and may implicate altered cell volume homeostasis. The aim of this study was to evaluate how cold preservation and rewarming affect hepatocellular volume regulation and concentrative amino acid uptake. METHODS:Rat hepatocytes suspended fo...
journal_title：Gastroenterology
pub_type： 杂志文章
doi：10.1016/s0016-5085(97)70148-3
更新日期：1997-04-01 00:00:00
abstract：BACKGROUND & AIMS:Abnormal processing of visceral sensation at the level of the central nervous system is believed to be involved in functional dyspepsia. However, compared with studies of stimulation-related changes in brain activity, few studies have focused on resting brain activity, which also is important in patho...
journal_title：Gastroenterology
pub_type： 杂志文章
doi：10.1053/j.gastro.2011.05.003
更新日期：2011-08-01 00:00:00
abstract：:Sweden has one of the highest incidences of gallstone disease in the Western world. It is therefore important to characterize the mechanisms responsible for the formation of cholesterol gallstones in this population. In the present study, we have determined the kinetics of the two primary bile acids, cholic acid and c...
journal_title：Gastroenterology
pub_type： 杂志文章
doi：10.1016/0016-5085(85)90328-2
更新日期：1985-08-01 00:00:00
abstract：BACKGROUND/AIMS:Staphylococcal enterotoxin B (SEB) acts as a superantigen binding to class II major histocompatibility complex proteins, and this complex stimulates T cells. The aim of this study was to investigate the pathogenic effects of SEB on hepatic injury and lethal shock in mice. METHODS:SEB was administered t...
journal_title：Gastroenterology
pub_type： 杂志文章
doi：10.1016/0016-5085(94)90604-1
更新日期：1994-02-01 00:00:00
abstract：:We studied gallbladder bile flow before, during, and after cholesterol gallstone formation in the prairie dog using infusion cholescintigraphy with 99mTc-diethyl iminodiacetic acid. In 18 fasting animals partitioning of bile between gallbladder and intestine was determined every 15 min for 140 min, and gallbladder res...
journal_title：Gastroenterology
pub_type： 杂志文章
doi：10.1016/0016-5085(86)90086-7
更新日期：1986-01-01 00:00:00
abstract：:The cause of Ménétrier's disease is unknown, although allergic, autoimmune, and infectious, particularly viral, causes have been postulated. This case report describes a 3-year-old child with Ménétrier's disease in whom evidence of acute cytomegalovirus (CMV) infection was found. To our knowledge, this is the first ca...
journal_title：Gastroenterology
pub_type： 杂志文章
doi：10.1016/0016-5085(95)90349-6
更新日期：1995-08-01 00:00:00
abstract：BACKGROUND & AIMS:Colonic crypt elongation occurs during both chronic colitis and in the recovery phase of acute colitis. The impact of these alterations on epithelial cell functions is not fully defined. METHODS:DNA microarray analysis of freshly isolated colonic epithelial cells (CECs) from acute and chronic colitis...
journal_title：Gastroenterology
pub_type： 杂志文章
doi：10.1016/s0016-5085(03)00665-6
更新日期：2003-07-01 00:00:00
abstract：BACKGROUND & AIMS:Previous research has yielded conflicting data as to whether the natural history of inflammatory bowel disease follows a seasonal pattern. The purpose of this study was to determine whether relapse of inflammatory bowel disease follows a seasonal pattern either across a cohort of patients or within in...
journal_title：Gastroenterology
pub_type： 杂志文章
doi：10.1053/j.gastro.2003.12.003
更新日期：2004-03-01 00:00:00
abstract：BACKGROUND & AIMS:The liver has high regenerative potential. We attempted to establish a novel culture system for extensive expansion of fetal mouse hepatic stem/progenitor cells and to characterize cultured cells. METHODS:Hepatic spheroids collected from 6-day floating cultures were cultured on collagen-coated dishes...
journal_title：Gastroenterology
pub_type： 杂志文章
doi：10.1053/j.gastro.2005.03.030
更新日期：2005-06-01 00:00:00
abstract：:In neurogenic fecal incontinence there is denervation of the external anal sphincter and pelvic floor muscles but the role of the internal anal sphincter is incompletely understood. We have evaluated the internal anal sphincter in 6 patients with neurogenic incontinence undergoing postanal repair and in 7 control subj...
journal_title：Gastroenterology
pub_type： 杂志文章
doi：10.1016/0016-5085(88)90175-8
更新日期：1988-10-01 00:00:00
abstract：:Weight gain and obesity are major risk factors for conditions and diseases ranging from insulin resistance and type 2 diabetes mellitus to atherosclerosis and the sequelae of nonalcoholic fatty liver disease. A chronic, subacute state of inflammation often accompanies the accumulation of excess lipid in adipose tissue...
journal_title：Gastroenterology
pub_type： 杂志文章,评审
doi：10.1053/j.gastro.2007.03.059
更新日期：2007-05-01 00:00:00
abstract：BACKGROUND/AIMS:The role of cholecystokinin (CCK) in the regulation of gastric acid secretion is still controversial. This study examined the effect of the CCK-A receptor antagonist loxiglumide (lox) on gastrin- or CCK-induced gastric acid secretion and meal-stimulated plasma gastrin levels in a placebo-controlled stud...
journal_title：Gastroenterology
pub_type： 临床试验,杂志文章
doi：10.1016/0016-5085(94)90799-4
更新日期：1994-12-01 00:00:00
abstract：:The influence of adaptive cell proliferation on colonic carcinogenesis was studied in male Fischer rats with a defunctioning transverse colostomy that was closed 4 wk later. Control observations were made in other rats after colonic transection, repeated at 4 wk, after laparotomy alone, or after permanent colostomy. T...
journal_title：Gastroenterology
pub_type： 杂志文章
doi：
更新日期：1981-09-01 00:00:00
abstract：:The assimilation of labeled cobalamin and the transport of corrinoids in portal blood, peripheral venous blood, and bile were studied in eight cholecystectomized patients, after ingestion of a dose of cyano[57Co]cobalamin (0.5 microCi). The radioactivity appeared in the portal vein after a delay of 1.5-2 hours and in ...
journal_title：Gastroenterology
pub_type： 杂志文章
doi：10.1016/0016-5085(91)90094-2
更新日期：1991-11-01 00:00:00
abstract：:The aim of the present study was to characterize rat mucosal colonic cells harvested from the crypt continuum during differentiation and dimethylhydrazine-induced neoplasia. The collection of colonocytes was performed using a modified nonenzymatic isolation procedure based on Ca2+ chelation and gentle mechanical disso...
journal_title：Gastroenterology
pub_type： 杂志文章
doi：10.1016/0016-5085(91)80013-y
更新日期：1991-03-01 00:00:00
abstract：:Gastric mucosa of the rat generates both PGE2 and PGI2 activity in significantly higher concentrations in the antrum than in the fundus. Aspirin, given intravenously or intragastrically in doses producing gastric mucosal lesions, causes a dose-dependent decrease in the generation of mucosal PGE2 and PGI2, which is fur...
journal_title：Gastroenterology
pub_type： 杂志文章
doi：
更新日期：1981-01-01 00:00:00
abstract：:Confluent monolayers of epithelial cell lines of intestinal derivation may serve as useful models for studies of intestinal epithelial structure and function. Cells of the T84 line appear to be a useful model for studies of Cl- secretion. We now define the fine structure of this cell line, the structural events that o...
journal_title：Gastroenterology
pub_type： 杂志文章
doi：10.1016/s0016-5085(87)91069-9
更新日期：1987-05-01 00:00:00
abstract：:The experimental production of gastric ulcers in dogs by subnucosal injection of ulcerogeic agents is described. The most satisfactory agent tested was 95% ethanol, because it produced immediate changes in the mucosa which delineated the size of the ulcer. The depth of ulceration could be controlled and the ulcer heal...
journal_title：Gastroenterology
pub_type： 杂志文章
doi：
更新日期：1975-07-01 00:00:00
abstract：BACKGROUND & AIMS:Chronic hepatitis C virus (HCV) infection is a major burden on individuals and health care systems. The Extension for Community Healthcare Outcomes (Project ECHO) enables primary care providers to deliver best-practice care for complex conditions to underserved populations. The US Congress passed the ...
journal_title：Gastroenterology
pub_type： 杂志文章
doi：10.1053/j.gastro.2017.10.016
更新日期：2017-12-01 00:00:00
abstract：:Recent data seem to support a tubular defect as the mechanism of the elevated renal clearance of amylase relative to creatinine in acute pancreatitis. Glucagon has been proposed by some to be an important factor in this phenomenon. To examine the role of glucagon as this "tubular dysfunction factor", we investigated t...
journal_title：Gastroenterology
pub_type： 临床试验,杂志文章
doi：
更新日期：1978-10-01 00:00:00
abstract：BACKGROUND/AIMS:The yeast Saccharomyces boulardii inhibits the secretion induced by cholera toxin (CT) in rat jejunum. The present study was aimed at unraveling the mechanism by which S. boulardii protects intestinal cells against CT. METHODS:CT-induced adenosine 3',5'-cyclic monophosphate (cAMP) levels were measured ...
journal_title：Gastroenterology
pub_type： 杂志文章
doi：10.1016/s0016-5085(94)94403-2
更新日期：1994-01-01 00:00:00
abstract：:In recent years the hypothesis that the number of villus cells regulates crypt cell proliferation in the epithelium of the small intestine has been brought forward by a number of investigators. To test this hypothesis, the villus cell population was reduced by clamping the superior mesenteric artery and vein in rats f...
journal_title：Gastroenterology
pub_type： 杂志文章
doi：
更新日期：1976-11-01 00:00:00
abstract：BACKGROUND:Growth failure frequently complicates the clinical course of inflammatory bowel disease (IBD) in children. This study was designed to investigate the role of disease activity versus steroid therapy on growth faltering in this disorder. METHODS:We studied growth failure and its relationship to disease activi...
journal_title：Gastroenterology
pub_type： 杂志文章
doi：10.1016/0016-5085(93)90883-e
更新日期：1993-09-01 00:00:00
abstract：:Regional specific antibodies and chromatography were used to analyze the distributions and molecular forms of peptide histidine isoleucine (PHI) and vasoactive intestinal peptide (VIP) in the porcine intestine. Both peptides were present along the entire length of the intestine, the highest concentrations occurring in...
journal_title：Gastroenterology
pub_type： 杂志文章
doi：10.1016/0016-5085(85)90445-7
更新日期：1985-09-01 00:00:00
abstract：:An enteroscopic method that uses a pediatric colonoscope provides an alternative approach to the diagnostic and management problems arising in the setting of a Roux-en-Y limb hepaticojejunostomy or biliary diversion. Three patients with Roux-en-Y limb reconstructions who experienced recurrent cholangitis, recurrent pa...
journal_title：Gastroenterology
pub_type： 杂志文章
doi：10.1016/0016-5085(88)90305-8
更新日期：1988-07-01 00:00:00
