Abstract:
:In vivo, IgE production is related to bronchial hyperresponsiveness and, in vitro, passive sensitization of human airways with asthmatic serum containing a high concentration of IgE enhances the contractile response to a variety of agonists. However, cell types implicated in this IgE sensitization are not fully determined. The aim of this study was to determine IgE-bearing cells during passive sensitization with special reference to mast cells. Peripheral bronchi were dissected out from 10 lung specimens obtained at thoracotomy and processed into glycolmethacrylate resin. Sections, each 2 microm thick, were passively sensitized by incubation for 2 h at 37 degrees C in either buffer supplemented with monoclonal IgE or asthmatic serum with a high concentration of IgE (> or = 1,000 IU/ml). Immunohistochemistry was performed using monoclonal antibodies directed against the epsilon chain, and markers of the various IgE-bearing cells (e.g., AA1, antichymase). The number of IgE-bearing cells was significantly higher in passively sensitized specimens as compared with nonsensitized specimens (6.63 +/- 1.71 versus 4.29 +/- 1.35/mm2; p = 0.013, n = 10). Mast cells represented 65% of IgE-bearing cells, 41.6 and 23.4% for TC and T subtypes, respectively. These results indicate that mast cell is the main cell type involved in IgE-induced passive sensitization. The involvement of mast cell-derived tryptase in the mechanisms of IgE-related hyperresponsiveness should be further examined.
journal_name
Am J Respir Crit Care Medauthors
Berger P,Walls AF,Marthan R,Tunon-de-Lara JMdoi
10.1164/ajrccm.157.2.9707042subject
Has Abstractpub_date
1998-02-01 00:00:00pages
610-6issue
2eissn
1073-449Xissn
1535-4970journal_volume
157pub_type
杂志文章abstract::The long-acting beta 2-agonist salmeterol has been suggested to have other pharmacologic activities, that is, antiinflammatory capacities, in addition to its bronchodilator properties. We investigated the protective effect of 50 micrograms salmeterol in a placebo-controlled study on house dust mite-induced early- and ...
journal_title:American journal of respiratory and critical care medicine
pub_type: 临床试验,杂志文章
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更新日期:1994-11-01 00:00:00
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journal_title:American journal of respiratory and critical care medicine
pub_type: 临床试验,杂志文章,随机对照试验
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更新日期:1997-12-01 00:00:00
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pub_type: 杂志文章
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journal_title:American journal of respiratory and critical care medicine
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更新日期:2006-10-15 00:00:00
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更新日期:2016-08-01 00:00:00
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pub_type: 杂志文章
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更新日期:2003-01-01 00:00:00
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更新日期:2007-10-01 00:00:00
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pub_type: 杂志文章,多中心研究
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更新日期:2015-06-15 00:00:00
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pub_type: 杂志文章
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更新日期:2006-09-01 00:00:00
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pub_type: 杂志文章
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更新日期:1999-06-01 00:00:00
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pub_type: 杂志文章,评审
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journal_title:American journal of respiratory and critical care medicine
pub_type: 杂志文章
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journal_title:American journal of respiratory and critical care medicine
pub_type: 杂志文章
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更新日期:1999-02-01 00:00:00
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更新日期:2020-05-15 00:00:00
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journal_title:American journal of respiratory and critical care medicine
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journal_title:American journal of respiratory and critical care medicine
pub_type: 临床试验,杂志文章,随机对照试验
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更新日期:1996-02-01 00:00:00
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journal_title:American journal of respiratory and critical care medicine
pub_type: 杂志文章
doi:10.1164/ajrccm.150.6.7952610
更新日期:1994-12-01 00:00:00
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更新日期:2005-06-15 00:00:00
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journal_title:American journal of respiratory and critical care medicine
pub_type: 杂志文章
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更新日期:2012-10-15 00:00:00
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更新日期:2009-08-01 00:00:00
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更新日期:2021-01-05 00:00:00