Abstract:
:We identified and characterized 14 extragenic mutations that suppressed the dominant egg-laying defect of certain lin-12 gain-of-function mutations. These suppressors defined seven genes: sup-17, lag-2, sel-4, sel-5, sel-6, sel-7 and sel-8. Mutations in six of the genes are recessive suppressors, whereas the two mutations that define the seventh gene, lag-2, are semi-dominant suppressors. These suppressor mutations were able to suppress other lin-12 gain-of-function mutations. The suppressor mutations arose at a very low frequency per gene, 10-50 times below the typical loss-of-function mutation frequency. The suppressor mutations in sup-17 and lag-2 were shown to be rare non-null alleles, and we present evidence that null mutations in these two genes cause lethality. Temperature-shift studies for two suppressor genes, sup-17 and lag-2, suggest that both genes act at approximately the same time as lin-12 in specifying a cell fate. Suppressor alleles of six of these genes enhanced a temperature-sensitive loss-of-function allele of glp-1, a gene related to lin-12 in structure and function. Our analysis of these suppressors suggests that the majority of these genes are part of a shared lin-12/glp-1 signal transduction pathway, or act to regulate the expression or stability of lin-12 and glp-1.
journal_name
Geneticsjournal_title
Geneticsauthors
Tax FE,Thomas JH,Ferguson EL,Horvitz HRsubject
Has Abstractpub_date
1997-12-01 00:00:00pages
1675-95issue
4eissn
0016-6731issn
1943-2631journal_volume
147pub_type
杂志文章相关文献
GENETICS文献大全abstract::There are two distinct reasons for making comparisons of genetic variation for quantitative characters. The first is to compare evolvabilities, or ability to respond to selection, and the second is to make inferences about the forces that maintain genetic variability. Measures of variation that are standardized by the...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1992-01-01 00:00:00
abstract::The type of variegation of eyes of white-mottled mutants of D. hydei, either small-spotted or large-spotted, depends on the specific chromosome rearrangement involved. This distinction between mutants, though handsome, is not absolute because very seldomly small-spotted types do show a larger pigment aggregate and som...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1973-10-01 00:00:00
abstract::The transcription factors WHITE COLLAR-1 (WC-1) and WHITE COLLAR-2 (WC-2) interact to form a heterodimeric complex (WCC) that is essential for most of the light-mediated processes in Neurospora crassa. WCC also plays a distinct non-light-related role as the transcriptional activator in the FREQUENCY (FRQ)/WCC feedback...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:2003-01-01 00:00:00
abstract::Complementation and polarity suppression data are interpreted in terms of the genetic structure of the maltose B region. It is proposed that this region comprises two divergent operons. One operon includes malK, a cistron involved in maltose permeation, and lamB the only known cistron specifically involved in lambda r...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1974-02-01 00:00:00
abstract::Promoter proximal pausing (PPP) of RNA polymerase II has emerged as a crucial rate-limiting step in the regulation of gene expression. Regulation of PPP is brought about by complexes 7SK snRNP, P-TEFb (Cdk9/cycT), and the negative elongation factor (NELF), which are highly conserved from Drosophila to humans. Here, we...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.120.303419
更新日期:2020-09-01 00:00:00
abstract::The results of deficiency mapping experiments reveal the presence of a compensatory response (cr+) locus that is located distal to the cluster of ribosomal RNA (rRNA) genes and is responsible for disproportionately replicating these genes when cr+ locus is present in a single dose, as in X/O males or X/sc4-sc8 females...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1978-01-01 00:00:00
abstract::Type II topoisomerases are essential ATP-dependent homodimeric enzymes required for transcription, replication, and chromosome segregation. These proteins alter DNA topology by generating transient enzyme-linked double-strand breaks for passage of one DNA strand through another. The central role of type II topoisomera...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.112.144006
更新日期:2012-11-01 00:00:00
abstract::Electrophoretic comparisons of 40S and 55S ribosomal subunit proteins from 18 strains of Drosophila melanogaster revealed the virtual absence of allelic variation. More detailed two-dimensional studies on the large subunit proteins in 6 of the strains demonstrated additional complexity but still no interstrain variati...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1974-12-01 00:00:00
abstract::The genetic basis of stochastic variation within a defined environment, and the consequences of such micro-environmental variance for fitness are poorly understood . Using a multigenerational breeding design in Drosophila serrata, we demonstrated that the micro-environmental variance in a set of morphological wing tra...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.116.199075
更新日期:2017-08-01 00:00:00
abstract::DNA sequence analysis and genetic mapping of loci from mating-type-specific chromosomes of the smut fungus Microbotryum violaceum demonstrated that the nonrecombining mating-type-specific region in this species comprises approximately 25% ( approximately 1 Mb) of the chromosome length. Divergence between homologous ma...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.109.103192
更新日期:2009-08-01 00:00:00
abstract::Understanding variation in allele frequencies across populations is a central goal of population genetics. Classical models for the distribution of allele frequencies, using forward simulation, coalescent theory, or the diffusion approximation, have been applied extensively for demographic inference, medical study des...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.117.200493
更新日期:2017-07-01 00:00:00
abstract::We present a two-layer hidden Markov model to detect the structure of haplotypes for unrelated individuals. This allows us to model two scales of linkage disequilibrium (one within a group of haplotypes and one between groups), thereby taking advantage of rich haplotype information to infer local ancestry of admixed i...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.113.160697
更新日期:2014-03-01 00:00:00
abstract::The Thomas Hunt Morgan Medal is awarded to an individual Genetics Society of America member for lifetime achievement in the field of genetics. It recognizes the full body of work of an exceptional geneticist. The 2016 recipient is Nancy Kleckner, who has made many significant contributions to our understanding of chro...
journal_title:Genetics
pub_type: 历史文章,杂志文章
doi:10.1534/genetics.116.197608
更新日期:2016-12-01 00:00:00
abstract::The Genetics Society of America (GSA) Medal recognizes researchers who have made outstanding contributions to the field of genetics in the past 15 years. The 2019 GSA Medal is awarded to Bonnie L. Bassler of Princeton University and the Howard Hughes Medical Institute in recognition of her groundbreaking studies of ba...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.120.303285
更新日期:2020-06-01 00:00:00
abstract::In most eukaryotes, crossovers are not independently distributed along the length of a chromosome. Instead, they appear to avoid close proximity to one another--a phenomenon known as crossover interference. Previously, for three of the five Arabidopsis chromosomes, we measured the strength of interference and suggeste...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.104.040055
更新日期:2005-06-01 00:00:00
abstract::Abnormalities in chromosome number that occurred during meiosis were evaluated with a specially-constructed diploid strain of Saccharomyces cerevisiae. The strain is heterozygous for six markers of the right arm of chromosome V and heterozygous for cyh2 (resistance to cycloheximide) on chromosome VII.-Selection of mei...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1982-05-01 00:00:00
abstract::This study concerns the problem of odor receptor gene choice in the fruit fly Drosophila melanogaster. From a family of 60 Odor receptor genes, only one or a small number are selected for expression by each olfactory receptor neuron. Little is known about how an olfactory receptor neuron selects a receptor, or how the...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.110.117622
更新日期:2010-09-01 00:00:00
abstract::Segregation data were obtained for 1260 potato linkage group I-specific AFLP loci from a heterozygous diploid potato population. Analytical tools that identified potential typing errors and/or inconsistencies in the data and that assembled cosegregating markers into bins were applied. Bins contain multiple-marker data...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:2003-12-01 00:00:00
abstract::The rdgB mutants depend on recombinational repair of double-strand breaks. To assess other consequences of rdgB inactivation in Escherichia coli, we isolated RdgB-dependent mutants. All transposon inserts making cells dependent on RdgB inactivate genes of double-strand break repair, indicating that chromosomal fragmen...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.105.051144
更新日期:2006-02-01 00:00:00
abstract::Several eukaryotic homologs of the Escherichia coli RecQ DNA helicase have been found. These include the human BLM gene, whose mutation results in Bloom syndrome, and the human WRN gene, whose mutation leads to Werner syndrome resembling premature aging. We cloned a Drosophila melanogaster homolog of the RECQ helicase...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1999-03-01 00:00:00
abstract::The need to regulate X chromosome expression in Caenorhabditis elegans arises as a consequence of the primary sex-determining signal, the X/A ratio (the ratio of X chromosomes to sets of autosomes), which directs 1X@A animals to develop as males and 2X/2A animals to develop as hermaphrodites. C. elegans possesses a do...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1994-08-01 00:00:00
abstract::A family of protein kinases regulates translation in response to different cellular stresses by phosphorylation of the alpha subunit of eukaryotic initiation factor-2 (eIF-2alpha). In yeast, an eIF-2alpha kinase, GCN2, functions in translational control in response to amino acid starvation. It is thought that uncharge...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:2000-02-01 00:00:00
abstract::Additional experiments with homologous as well as heterologous hybridization confirmed our previous finding in Sciara coprophila that XX females have nearly twice the number of ribosomal RNA cistrons as XO males. A comparison between two different X' chromosomes revealed that only the one carrying the irradiation-indu...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1976-05-01 00:00:00
abstract::In contrast to our growing understanding of patterns of additive genetic variance in single- and multi-trait combinations, the relative contribution of nonadditive genetic variance, particularly dominance variance, to multivariate phenotypes is largely unknown. While mechanisms for the evolution of dominance genetic v...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.115.175489
更新日期:2015-05-01 00:00:00
abstract::Reproductive isolation that initiates speciation is likely caused by incompatibility among multiple loci in organisms belonging to genetically diverging populations. Laboratory C57BL/6J mice, which predominantly originated from Mus musculus domesticus, and a MSM/Ms strain derived from Japanese wild mice (M. m. molossi...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.106.062976
更新日期:2007-01-01 00:00:00
abstract::Genome-wide association studies have identified thousands of variants implicated in dozens of complex diseases. Most studies collect individuals with and without disease and search for variants with different frequencies between the groups. For many of these studies, additional disease traits are also collected. Joint...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.116.198473
更新日期:2017-03-01 00:00:00
abstract::A previously employed method that uses the composition of noncoding DNA as the basis of a test for selection between synonymous codons in plastid genes is reevaluated. The test requires the assumption that in the absence of selective differences between synonymous codons the composition of silent sites in coding seque...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:2001-09-01 00:00:00
abstract::The binding of alpha-factor to its receptor (Ste2p) activates a G-protein-signaling pathway leading to conjugation of MATa cells of the budding yeast S. cerevisiae. We conducted a genetic screen to identify constitutively activating mutations in the N-terminal region of the alpha-factor receptor that includes transmem...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:2002-02-01 00:00:00
abstract::The recessive mutation, hop1-1, was isolated by use of a screen designed to detect mutations defective in homologous chromosomal pairing during meiosis in Saccharomyces cerevisiae. Mutants in HOP1 displayed decreased levels of meiotic crossing over and intragenic recombination between markers on homologous chromosomes...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1989-03-01 00:00:00
abstract::To identify genes regulating the development of the six touch receptor neurons, we screened the F(2) progeny of mutated animals expressing an integrated mec-2::gfp transgene that is expressed mainly in these touch cells. From 2638 mutated haploid genomes, we obtained 11 mutations representing 11 genes that affected th...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:2001-05-01 00:00:00