Abstract:
:The RAD54 gene, which encodes a protein in the SWI2/SNF2 family, plays an important role in recombination and DNA repair in Saccharomyces cerevisiae. The yeast genome project revealed a homologue of RAD54, RDH54/TID1. Properties of the rdh54/tid1 mutant and the rad54 rdh54/tid1 double mutant are shown for mitosis and meiosis. The rad54 mutant is sensitive to the alkylating agent, methyl methanesulfonate (MMS), and is defective in interchromosomal and intrachromosomal gene conversion. The rdh54/tid1 single mutant, on the other hand, does not show any significant deficiency in mitosis. However, the rad54 rdh54/tid1 mutant is more sensitive to MMS and more defective in interchromosomal gene conversion than is the rad54 mutant, but shows the same frequency of intrachromosomal gene conversion as the rad54 mutant. These results suggest that RDH54/TID1 is involved in a minor pathway of mitotic recombination in the absence of R4D54. In meiosis, both single mutants produce viable spores at slightly reduced frequency. However, only the rdh54/tid1 mutant, but not the rad54 mutant, shows significant defects in recombination: retardation of the repair of meiosis-specific double-strand breaks (DSBs) and delayed formation of physical recombinants. Furthermore, the rad54 rdh54/tid1 double mutant is completely defective in meiosis, accumulating DSBs with more recessed ends than the wild type and producing fewer physical recombinants than the wild type. These results suggest that one of the differences between the late stages of mitotic recombination and meiotic recombination might be specified by differential dependency on the Rad54 and Rdh54/Tid1 proteins.
journal_name
Geneticsjournal_title
Geneticsauthors
Shinohara M,Shita-Yamaguchi E,Buerstedde JM,Shinagawa H,Ogawa H,Shinohara Asubject
Has Abstractpub_date
1997-12-01 00:00:00pages
1545-56issue
4eissn
0016-6731issn
1943-2631journal_volume
147pub_type
杂志文章相关文献
GENETICS文献大全abstract::Mismatch repair of palindromic loops in the presence or absence of single-base mismatches was investigated in wild-type and mismatch-binding defective mutant Chinese hamster ovary cells. Recombination intermediates with a maximum heteroduplex DNA (hDNA) region of 697 bp contained a centrally located, phenotypically si...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1998-03-01 00:00:00
abstract::Neural ganglia of wild type third-instar larvae of Drosophila melanogaster were incubated for 13 hours at various concentrations of BUdR (1, 3, 9, 27 micrograms/ml). Metaphases were collected with colchicine, stained with Hoechst 33258, and scored under a fluorescence microscope. Metaphases in which the sister chromat...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1979-02-01 00:00:00
abstract::The back-to-back geometry of sister kinetochores is essential in preventing loss or damage of chromosomes during mitosis. Kinetochore orientation is generated in part by a process of resolving kinetochores at the centromere (centromere resolution) prior to spindle interactions. Because few of the genes required for ce...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.107.075275
更新日期:2008-01-01 00:00:00
abstract::A continuing study of mortality rates among children born to survivors of the atomic bombings and a suitable group of controls has been updated; the average interval between birth and verification of death or survival is 17 years. The mortality experience is now based on 18,946 children liveborn to parents one or both...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1974-02-01 00:00:00
abstract::A laboratory strain of Drosophila pseudoobscura (L116) is studied that, when crossed to sex-ratio homozygous females, produces sons that exhibit varying levels of the male sex-ratio (msr) phenotype. The msr phenotype occurs only in sex-ratio males and is due to the production of a high frequency of nullo-XY sperm. The...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1986-06-01 00:00:00
abstract::Disequilibrium pattern analysis, a general method for analyzing evolutionary events acting on pairs of tightly linked polymorphic loci, is applied to a large sample of Danish individuals typed for A and B loci of the HLA (human leukocyte antigen) system. Cases of selection on particular haplotypes are revealed from pa...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1987-08-01 00:00:00
abstract::The mak7-1 mutant loses the killer toxin-encoding M1 dsRNA. MAK7 is RPL4A, one of two genes encoding ribosomal protein L4. KRB1 is a dominant suppressor of mak7-1 that is tightly centromerelinked, but not linked to centromere markers of chromosomes I-XVI. Our orthogonal field agarose gel electrophoresis analysis of ch...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1995-05-01 00:00:00
abstract::Clf1p is an essential, highly conserved protein in S. cerevisiae that has been implicated in pre-mRNA splicing. Clf1p's ortholog in Drosophila, Crn, is required for normal cell proliferation. Cells depleted of Clf1p arrest primarily with large buds, a single nucleus, a 2C DNA content, and a short, intact mitotic spind...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:2002-04-01 00:00:00
abstract::Allozyme and inversion data from natural populations of Drosophila melanogaster from the eastern United States were analyzed to determine whether the clines at allozyme loci are due to nonrandom associations with common cosmopolitan inversions. All inversions show strong clines. Clines were large and significant for h...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1978-03-01 00:00:00
abstract::The white-apricot (wa) allele differs from the wild-type white gene by the presence of the retrovirus-like transposable element copia within the transcription unit. Most RNAs derived from wa have 3' termini within this insertion, and only small amounts of structurally normal RNA are produced. The activity of wa is red...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1990-12-01 00:00:00
abstract::The larval body wall muscles of Drosophila melanogaster arise by fusion of founder myoblasts (FMs) and fusion-competent myoblasts (FCMs). Sticks-and-Stones (SNS) is expressed on the surface of all FCMs and mediates adhesion with FMs and developing syncytia. Intracellular components essential for myoblast fusion are th...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.107.083808
更新日期:2008-03-01 00:00:00
abstract::To understand the role of Los1p in pre-tRNA splicing, we sought los1 multicopy suppressors. We found SOL1 that suppresses both point and null LOS1 mutations. Since, when fused to the Ga14p DNA-binding domain, Los1p activates transcription, we tested whether Los1p regulates SOL1. We found that las1 mutants have deplete...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1996-06-01 00:00:00
abstract::Strong deviation of allele frequencies from Mendelian inheritance favoring Mus spretus-derived alleles has been described previously for X-linked loci in four mouse interspecific crosses. We reanalyzed data for three of these crosses focusing on the location of the gene(s) controlling deviation on the X chromosome and...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1996-08-01 00:00:00
abstract::To reveal phenotypic and functional genomic patterns of mitonuclear adaptation, a laboratory adaptation study with Caenorhabditis elegans nematodes was conducted in which independently evolving lines were initiated from a low-fitness mitochondrial electron transport chain (ETC) mutant, gas-1 Following 60 generations o...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.119.301935
更新日期:2019-03-01 00:00:00
abstract::l(3)S12 is a vital locus whose function is required in embryos, early larvae, late pupae and oogenesis. We have identified a cold-sensitive allele, l(3)S12(3), and characterized conditional misexpression of the gene associated with this mutation as well as with several euchromatic insertions of l(3)S12+ transposons. S...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1991-05-01 00:00:00
abstract::Analysis of raw pooled data from distinct studies of a single question generates a single statistical conclusion with greater power and precision than conventional metaanalysis based on within-study estimates. However, conducting analyses with pooled genetic data, in particular, is a daunting task that raises importan...
journal_title:Genetics
pub_type: 杂志文章,meta分析
doi:
更新日期:2001-11-01 00:00:00
abstract::The fixation probability is determined when population size and selection change over time and differs from Kimura's result, with long-term implications for a population. It is found that changes in population size are not equivalent to the corresponding changes in selection and can result in less drift than anticipat...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.111.129288
更新日期:2011-08-01 00:00:00
abstract::The formation and stability of epigenetically regulated chromatin is influenced by DNA replication and factors that modulate post-translational modifications on histones. Here we describe evidence that PCNA can affect silencing in Saccharomyces cerevisiae by facilitating deposition of H3 K56ac onto chromosomes. We pro...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.107.084525
更新日期:2008-06-01 00:00:00
abstract::An approximately 135-bp sequence called the A1/A2 repeat was isolated from the transcribed region of the 26-18S rDNA intergenic spacer (IGS) of Nicotiana tomentosiformis. Fluorescence in situ hybridization (FISH) and Southern blot analysis revealed its occurrence as an independent satellite (termed an A1/A2 satellite)...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.166.4.1935
更新日期:2004-04-01 00:00:00
abstract::Cyclic guanosine monophosphate (cGMP) is a key secondary messenger used in signal transduction in various types of sensory neurons. The importance of cGMP in the ASE gustatory receptor neurons of the nematode Caenorhabditis elegans was deduced by the observation that multiple receptor-type guanylyl cyclases (rGCs), en...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.113.152660
更新日期:2013-08-01 00:00:00
abstract::Understanding the transmission dynamics of infectious diseases is important for both biological research and public health applications. It has been widely demonstrated that statistical modeling provides a firm basis for inferring relevant epidemiological quantities from incidence and molecular data. However, the comp...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.115.180034
更新日期:2016-03-01 00:00:00
abstract::The genomic prediction of phenotypes and breeding values in animals and plants has developed rapidly into its own research field. Results of genomic prediction studies are often difficult to compare because data simulation varies, real or simulated data are not fully described, and not all relevant results are reporte...
journal_title:Genetics
pub_type: 杂志文章,评审
doi:10.1534/genetics.112.147983
更新日期:2013-02-01 00:00:00
abstract::Laterality is believed to have genetic components, as has been deduced from family studies in humans and responses to artificial selection in mice, but these genetic components are unknown and the underlying physiological mechanisms are still a subject of dispute. We measured direction of laterality (preferential use ...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:2003-03-01 00:00:00
abstract::Notch signaling pathways can be regulated through a variety of cellular mechanisms, and genetically compromised systems provide useful platforms from which to search for the responsible modulators. The Caenorhabditis elegans gene aph-1 encodes a component of γ-secretase, which is essential for Notch signaling events t...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.111.136804
更新日期:2012-03-01 00:00:00
abstract::The decrease of meiotic exchanges (crossing over and conversion) in two mutants of Sordaria macrospora correlated strongly with a reduction of chiasmata and of both types of "recombination nodules." Serial section reconstruction electron microscopy was used to compare the synapsis pattern of meiotic prophase I in wild...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1992-09-01 00:00:00
abstract::Many insects feed on only one or a few types of host. These host specialists often evolve a preference for chemical cues emanating from their host and develop mechanisms for circumventing their host's defenses. Adaptations like these are central to evolutionary biology, yet our understanding of their genetics remains ...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.113.154773
更新日期:2013-11-01 00:00:00
abstract::Genes with unstable transcripts often encode proteins that play important regulatory roles. ATL2 is a member of a multigene family coding highly related RING-H2 zinc-finger proteins that may function as E3 ubiquitin ligases. ATL2 mRNA accumulation occurs rapidly and transiently after incubation with elicitors of patho...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.104.028043
更新日期:2004-06-01 00:00:00
abstract::Gene silencing mediated by dsRNA (RNAi) can persist for multiple generations in Caenorhabditis elegans (termed RNAi inheritance). Here we describe the results of a forward genetic screen in C. elegans that has identified six factors required for RNAi inheritance: GLH-1/VASA, PUP-1/CDE-1, MORC-1, SET-32, and two novel ...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.116.198812
更新日期:2017-07-01 00:00:00
abstract::In Xiphophorus, the causative genetic information for melanoma formation has been assigned by classical genetics to chromosomal loci, which are located on the sex chromosomes. In our attempts to molecularly clone these melanoma-determining loci, named Tu, we have looked for restriction-fragment-length markers (RFLMs) ...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1988-07-01 00:00:00
abstract::The distinction between deleterious, neutral, and adaptive mutations is a fundamental problem in the study of molecular evolution. Two significant quantities are the fraction of DNA variation in natural populations that is deleterious and destined to be eliminated and the fraction of fixed differences between species ...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:2001-07-01 00:00:00