Abstract:
:Insulin-like growth factor-I (IGF-I) is involved in the growth and development of liver and brain during fetal life by acting through specific plasma membrane receptors. In an attempt to determine how the changes in IGF-I receptor number are regulated during development, we have compared [125I]IGF-I binding to membrane fractions with the concentration of IGF-I receptor mRNA in rat liver and brain. IGF-I binding to liver membranes was 4.5 times higher in 20-day-old fetuses than in adult rats. After partial hepatectomy (70%) in adult rats a transient 2-fold increase of IGF-I binding to liver membranes was observed. In fetal and regenerating liver increases similar to those observed for IGF-I binding were observed in IGF-I receptor mRNA concentrations. In brain microsomes IGF-I binding was 3.5 times higher in 20-day-old fetuses than in adults. This difference reflects a similar change in the number of IGF-I receptors without modifications in the affinity of the receptor for the ligand. In contrast to the liver, no significant changes in the concentration of IGF-I receptor mRNA were observed in the developing brain when determined by hybridization solution, Northern blot or RNase protection analysis. These findings suggest that in the liver, the IGF-I receptor gene is regulated at pretranslational level during development and regeneration, while in brain it is preferentially regulated at translational or posttranslational level.
journal_name
Mol Cell Endocrinoljournal_title
Molecular and cellular endocrinologyauthors
Santos A,Yusta B,Fernández-Moreno MD,Blázquez Edoi
10.1016/0303-7207(94)90222-4subject
Has Abstractpub_date
1994-05-01 00:00:00pages
85-93issue
1-2eissn
0303-7207issn
1872-8057journal_volume
101pub_type
杂志文章abstract::While the transcriptional effects of glucocorticoid hormone manipulation on the pituitary pro-opiomelanocortin (POMC) gene have been documented, it is not yet clear whether glucocorticoids activate additional post-transcriptional mechanisms to regulate POMC gene expression. We have used RNA probes that span exon/intro...
journal_title:Molecular and cellular endocrinology
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journal_title:Molecular and cellular endocrinology
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pub_type: 杂志文章
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journal_title:Molecular and cellular endocrinology
pub_type: 杂志文章
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pub_type: 杂志文章
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