Abstract：
:The cdc19+ gene encodes an essential member of the MCM family of replication proteins in Schizosaccharomyces pombe. We have examined the structure and function of the Cdc19p protein using molecular and genetic approaches. We find that overproduction of wild-type Cdc19p in wild-type cells has no effect, but cdc19-P1 mutant cells do not tolerate elevated levels of other MCM proteins or overexpression of mutant forms of Cdc19p. We have found genetic interactions between cdc19+ and genes encoding subunits of DNA polymerase delta and the replication initiator cdc18+. We have constructed a series of point mutations and sequence deletions throughout Cdc19p, which allow us to distinguish essential from nonessential regions of the protein. Not surprisingly, conserved residues in the MCM homology domain are required for protein function, but some residues outside the core homology domain are dispensable.
journal_name
Geneticsjournal_title
Geneticsauthors
Forsburg SL,Sherman DA,Ottilie S,Yasuda JR,Hodson JAsubject
Has Abstractpub_date
1997-11-01 00:00:00pages
1025-41issue
3eissn
0016-6731issn
1943-2631journal_volume
147pub_type
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