Abstract:
:Human amniotic epithelial (HAE) cells have been used for allotransplantation in patients with lysosomal storage disease due to lack of expression of HLA antigens. Previously, we have reported the expression of differentiation markers for both neural stem cells, and neuron and glial cells. In the present study, we investigated the presence of choline acetyltransferase (ChAT) and acetylcholine (ACh) in HAE cells using different experimental approaches. Cultured HAE cells showed strong immunoreactivity against ChAT antibody. ChAT activity in primary cells was 24.9 +/- 8.5 pmol/mg protein/h. Using HPLC with electrochemical detection, ACh was detected in both cell incubation media and cell pellets indicating that these cells synthesize and release ACh in a time-dependent manner. Additional confirmation of this hypothesis was gained from the data obtained from RT-PCR and Western blot analyses which revealed the expression of ChAT mRNA and ChAT protein, respectively, in HAE cells. Results of the present study suggest that HAE cells can possibly be applied for intracerebral allografting to treat neurologic diseases in which cholinergic neurons are damaged.
journal_name
Neurosci Lettjournal_title
Neuroscience lettersauthors
Sakuragawa N,Misawa H,Ohsugi K,Kakishita K,Ishii T,Thangavel R,Tohyama J,Elwan M,Yokoyama Y,Okuda O,Arai H,Ogino I,Sato Kdoi
10.1016/s0304-3940(97)00570-3subject
Has Abstractpub_date
1997-08-22 00:00:00pages
53-6issue
1eissn
0304-3940issn
1872-7972pii
S0304-3940(97)00570-3journal_volume
232pub_type
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