Abstract:
:The "parallel-up" packing in cellulose Ialpha and Ibeta unit cells was experimentally demonstrated by a combination of direct-staining the reducing ends of cellulose chains and microdiffraction-tilting electron crystallographic analysis. Microdiffraction investigation of nascent bacterial cellulose microfibrils showed that the reducing end of the growing cellulose chains points away from the bacterium, and this provides direct evidence that polymerization by the cellulose synthase takes place at the nonreducing end of the growing cellulose chains. This mechanism is likely to be valid also for a number of processive glycosyltransferases such as chitin synthases, hyaluronan synthases, and proteins involved in the synthesis of nodulation factor backbones.
journal_name
Proc Natl Acad Sci U S Aauthors
Koyama M,Helbert W,Imai T,Sugiyama J,Henrissat Bdoi
10.1073/pnas.94.17.9091subject
Has Abstractpub_date
1997-08-19 00:00:00pages
9091-5issue
17eissn
0027-8424issn
1091-6490journal_volume
94pub_type
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