Parallel-up structure evidences the molecular directionality during biosynthesis of bacterial cellulose.

Abstract:

:The "parallel-up" packing in cellulose Ialpha and Ibeta unit cells was experimentally demonstrated by a combination of direct-staining the reducing ends of cellulose chains and microdiffraction-tilting electron crystallographic analysis. Microdiffraction investigation of nascent bacterial cellulose microfibrils showed that the reducing end of the growing cellulose chains points away from the bacterium, and this provides direct evidence that polymerization by the cellulose synthase takes place at the nonreducing end of the growing cellulose chains. This mechanism is likely to be valid also for a number of processive glycosyltransferases such as chitin synthases, hyaluronan synthases, and proteins involved in the synthesis of nodulation factor backbones.

authors

Koyama M,Helbert W,Imai T,Sugiyama J,Henrissat B

doi

10.1073/pnas.94.17.9091

subject

Has Abstract

pub_date

1997-08-19 00:00:00

pages

9091-5

issue

17

eissn

0027-8424

issn

1091-6490

journal_volume

94

pub_type

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