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Purification and biochemical characterization of a virus-specific reverse transcriptase from human osteosarcoma tissue.

Abstract：

:A RNA-dependent DNA polymerase (RTase) was purified from human osteosarcoma tissue by successive column chromatography of the microsomal fraction on DEAE-cellulose (DE-23 and DE-52) and phosphocellulose. The purified enzyme has a molecular weight of about 68,000, a pH optimum of 8.1, a Mg2+ optimum of 0.8 mM, Mn2+ optimum of 1.0 mM and a KCl optimum of 60 mM. The enzyme transcribes (rA)n . (dT)12, (rC)n . (dG)12-18 and (2-O-methyl C)n . (dG)18, but is unable to transcribe (dA)n . (dT)10. The enzyme has no catalytic activity in the presence of oligodeoxynucleotide initiators alone, indicating the absence of terminal deoxynucleotidyl transferase. The purified enzyme is able to transcribe the heteropolymeric regions of a 70S RNA from R(Mu)LV. The presented data support the presence of a RNA-dependent DNA polymerase in human osteosarcoma tissue with biochemical properties, resembling those of C-type RNA tumor viruses.

journal_name

Cancer Lett

journal_title

Cancer letters

authors

Ebener U,Welte K,Chandra P

doi

10.1016/s0304-3835(79)80078-6

subject

Has Abstract

pub_date

1979-08-01 00:00:00

pages

179-88

issue

4

eissn

0304-3835

issn

1872-7980

pii

S0304-3835(79)80078-6

journal_volume

7

pub_type

杂志文章

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