Abstract:
:Antibodies to double-stranded DNA (anti-dsDNA, dsDNA-Ab) are frequently found in systemic lupus erythematosus (SLE), especially during active disease and differ with respect to immunoglobulin class and avidity. The detection of anti-dsDNA is one of the diagnostic criteria for SLE according to the American College of Rheumatology (ACR). Most of the commercial ELISA test systems have great advantages in routine laboratory testing but often detect dsDNA-Ab which are not specific for SLE and therefore give false positive results for non-SLE patients. The newly developed ELISA presented here, using human recombinant dsDNA (h-Rek) is compared to two commercial ELISA tests with genomic dsDNA from salmon testes (L-dsDNA) or plasmid dsDNA (P-dsDNA) and to the Chrithidia luciliae immunofluorescence test (CLIF) as well. In this study 143 sera were tested, 48 derived from patients with SLE, 40 from rheumatoid arthritis patients, 26 from non-rheumatoid patients whose sera were ANA-negative but L-dsDNA-Ab-positive and 30 from healthy volunteers. All patients were followed and clinically defined by the rheumatology outpatient clinic of our hospital. The prevalence for SLE of all sera was 32%. The sensitivity was 0.73 (h-Rek), 0.83 (L-dsDNA), 0.81 (P-dsDNA) and 0.57 (CLIF); specificity was determined 0.84 (h-Rek), 0.62 (L-dsDNA), 0.63 (P-dsDNA) and 0.98 (CLIF). The diagnostic efficiency of the L-dsDNA- and P-dsDNA-assay was identical, 0.69, and amounted to 0.81 for the h-Rek and 0.84 for the CLIF. Comparing all the ELISA tests and CLIF, the human recombinant dsDNA ELISA is much more sensitive than the CLIF, but considerably more specific than the ELISA assays using genomic or plasmid DNA, whereas the diagnostic efficiency is very close to that of the CLIF. This new generation of anti-dsDNA ELISA using human recombinant dsDNA seems to be a much better diagnostic tool for the detection of highly specific anti-dsDNA antibodies in the diagnosis of SLE than other commercial ELISAs. These results can only be explained by the use of a human recombinant antigen instead of undefined genomic or recombinant plasmid DNA for immobilization.
journal_name
Z Rheumatoljournal_title
Zeitschrift fur Rheumatologieauthors
Wigand R,Gottschalk R,Falkenbach A,Matthias T,Kaltwasser JP,Hoelzer Ddoi
10.1007/s003930050020subject
Has Abstractpub_date
1997-03-01 00:00:00pages
53-62issue
2eissn
0340-1855issn
1435-1250journal_volume
56pub_type
杂志文章abstract:BACKGROUND:Sex-specific differences in the prevalence and severity of immune disorders are well-known phenomena; however, it is only recently that we have begun to understand the possible causes of such differences. MATERIAL AND METHODS:A literature search on this topic was carried out and the results are summarized. ...
journal_title:Zeitschrift fur Rheumatologie
pub_type: 杂志文章,评审
doi:10.1007/s00393-014-1357-4
更新日期:2014-09-01 00:00:00
abstract::The neonatal lupus erythematosus syndrome, first described by McCuistion and Schoch in 1954, is associated with characteristic skin lesions and congenital heart block in the new-born, and the presence of Ro-(SSA), La-(SSB), or RNP antibodies in mothers and infants. A transplacental transference of maternal autoantibod...
journal_title:Zeitschrift fur Rheumatologie
pub_type: 杂志文章,评审
doi:
更新日期:1989-03-01 00:00:00
abstract::Antineutrophil cytoplasmic antibody (ANCA)-associated vasculitides (AAV) require a differentiated therapeutic approach depending on the degree of organ dysfunction and disease activity. In organ dysfunction and life-threatening AAV cyclophosphamide and rituximab are recommended for the induction of remission. For remi...
journal_title:Zeitschrift fur Rheumatologie
pub_type: 杂志文章,评审
doi:10.1007/s00393-016-0241-9
更新日期:2017-02-01 00:00:00
abstract:AIM:To gather information about current structures in rheumatologic ambulatory health care in Germany. Based on the results recommendations on future structures will be evaluated. METHODS:Data collection and statistical analysis via a structured 10-page questionnaire answered by members of the German Association of Rh...
journal_title:Zeitschrift fur Rheumatologie
pub_type: 杂志文章
doi:10.1007/s00393-007-0190-4
更新日期:2007-11-01 00:00:00
abstract::This article gives a comprehensive overview of diffuse interstitial lung diseases especially those associated with connective tissue diseases. Interstitial lung diseases (ILD) represent a heterogeneous group of diseases of very different and partially unknown etiologies. Therefore, an exact classification of the ILD i...
journal_title:Zeitschrift fur Rheumatologie
pub_type: 杂志文章,评审
doi:10.1007/s00393-003-0485-z
更新日期:2003-04-01 00:00:00
abstract::The author describes the information gained from anamnesis which points to a psychogenetic cause of rheumatic complaints. These symptoms are part of the diagnostically important criteria which must be supplemented still further by means of a personal discussion with the patient. A great deal depends on the skill of th...
journal_title:Zeitschrift fur Rheumatologie
pub_type: 杂志文章
doi:
更新日期:1993-07-01 00:00:00
abstract::Polyarteritis nodosa (PAN) is a necrotizing vasculitis of medium size arteries that may affect various organs. The clinical appearance is very variable. The most common manifestations are of the skin, the peripheral nervous system presenting as mononeuritis multiplex and the mesenteric and renal blood vessels due to t...
journal_title:Zeitschrift fur Rheumatologie
pub_type: 杂志文章
doi:10.1007/s00393-014-1483-z
更新日期:2014-12-01 00:00:00
abstract::Personalized healthcare tries to predict the effectiveness and safety in individual therapeutic approaches by defining individual patient characteristics (biomarkers), while stratification uses diagnostic patterns for groups of patients for prediction of therapeutic response with less adverse reactions caused by the d...
journal_title:Zeitschrift fur Rheumatologie
pub_type: 杂志文章
doi:10.1007/s00393-011-0886-3
更新日期:2013-02-01 00:00:00
abstract:BACKGROUND:Systemic sclerosis (SSc) is characterized by heterogeneous clinical symptoms. Peripheral skin fibrosis can be a common symptom. Nevertheless, a variety of diseases with different etiologies are associated with a thickening of the skin and make the initial diagnosis of systemic sclerosis more difficult. OBJE...
journal_title:Zeitschrift fur Rheumatologie
pub_type: 杂志文章,评审
doi:10.1007/s00393-018-0538-y
更新日期:2019-02-01 00:00:00
abstract::The revised S2 guidelines for treatment of giant cell arteritis have recently been published. Glucocorticosteroids remain the standard first line treatment. For severe or relapsing courses of the disease, the IL‑6 antagonist tocilizumab, a potent antibody, is now available as a therapeutic option; however, how long th...
journal_title:Zeitschrift fur Rheumatologie
pub_type: 杂志文章
doi:10.1007/s00393-020-00947-4
更新日期:2020-12-22 00:00:00
abstract::Arthrosis deformans is a common disease worldwide with a high prevalence among all ethnic groups. All joints may be affected and genetic factors influence the onset of the disease. The main symptoms include pain and increasing immobility over the course of the illness. Treatment in early stages is conservative. In the...
journal_title:Zeitschrift fur Rheumatologie
pub_type: 杂志文章,评审
doi:10.1007/s00393-008-0304-7
更新日期:2008-07-01 00:00:00
abstract::A few days after the SARS-CoV-2 infection was declared a pandemic, the German Society for Rheumatology (DGRh) compiled first recommendations for the care of patients with inflammatory rheumatic diseases (IRD). These first recommendations were based on an expert consensus and were largely non-evidence-based. Now that t...
journal_title:Zeitschrift fur Rheumatologie
pub_type: 杂志文章,实务指引,评审
doi:10.1007/s00393-020-00851-x
更新日期:2020-09-01 00:00:00
abstract:OBJECTIVE:To investigate the association between HLA‑G polymorphisms and systemic lupus erythematosus (SLE) susceptibility as well as the relationship between circulating soluble HLA‑G (sHLA‑G) levels and SLE. METHODS:A meta-analysis was performed to investigate the relationships between HLA‑G 14-bp insertion (I)/dele...
journal_title:Zeitschrift fur Rheumatologie
pub_type: 杂志文章
doi:10.1007/s00393-020-00783-6
更新日期:2020-04-09 00:00:00
abstract::Impaired host immunity has been regarded as a predisposing factor in post-primary tuberculosis in adults. Patients with systemic lupus erythematosus (SLE) are usually exposed to high doses of corticosteroids and eventually develop defective cellular immunity that increases the risk for active tuberculosis. SLE-associa...
journal_title:Zeitschrift fur Rheumatologie
pub_type: 杂志文章
doi:10.1007/s00393-003-0492-0
更新日期:2003-12-01 00:00:00
abstract::In recent years German authorities established a global budget for health care expenditures solely based on macroeconomic aspects. In the last year there have been attempts to define differentiated budgets for chronic diseases. These plans are mainly defined by quality aspects on the process level (creating networks) ...
journal_title:Zeitschrift fur Rheumatologie
pub_type: 杂志文章
doi:10.1007/s003930050134
更新日期:1998-12-01 00:00:00
abstract::Peripheral blood lymphocytes of patients with rheumatoid arthritis, psoriatic arthritis and healthy controls, isolated by a ficoll density gradient, were incubated with different concentrations of histamine, cimetidine and diphenhydramine (DPH) and simultaneously stimulated with phytohemagglutinine (PHA). In compariso...
journal_title:Zeitschrift fur Rheumatologie
pub_type: 杂志文章
doi:
更新日期:1984-07-01 00:00:00
abstract::The painter Max Slevogt (1868-1932), together with Lovis Corinth and Max Liebermann, was one of the most important representatives of German impressionism. Slevogt, a gourmet who appreciated fine food and good wines, suffered from acute recurrent gouty arthritis starting at the age of 27 years. His medical history is ...
journal_title:Zeitschrift fur Rheumatologie
pub_type: 传,历史文章,杂志文章
doi:10.1007/s00393-019-00730-0
更新日期:2020-06-01 00:00:00
abstract::Among the rheumatic diseases, systemic lupus erythematosus (SLE) reveals the highest frequency of central nervous symptoms. Psychiatric abnormalities are present in over 90% of the patients, if very mild cognitive impairment is included. The psychiatric syndromes listed by the American College of Rheumatologists encom...
journal_title:Zeitschrift fur Rheumatologie
pub_type: 杂志文章,评审
doi:10.1007/s00393-004-0618-z
更新日期:2004-04-01 00:00:00
abstract::A central problem of persons with chronic polyarthritis is the necessary adaptation to deteriorating health in everyday activities. Difficulties and burdens of this adaptation are parts of the study undertaken in the Unna Model, which was part of the research on "Local Services for CP-Patients". In the first part two ...
journal_title:Zeitschrift fur Rheumatologie
pub_type: 杂志文章
doi:
更新日期:1992-01-01 00:00:00
abstract::Chondral or osteochondral lesions are typical injuries in orthopaedics and traumatology. Since there is no regeneration of damaged articular cartilage, these lesions can lead to premature osteoarthritis. Therefore, an adequate therapy for these injuries is an important goal. Nowadays, common methods in cartilage thera...
journal_title:Zeitschrift fur Rheumatologie
pub_type: 杂志文章
doi:10.1007/s00393-007-0213-1
更新日期:2007-10-01 00:00:00
abstract::Seronegative rheumatoid arthritis (RA) patients have increased proportions of OKT-4+ (helper) T cells and diminished proportions of OKT-8+ (suppressor/cytotoxic) T cells in the peripheral blood. This phenomenon corresponds to diminished inhibition of B cell activation by peripheral blood lymphocytes (PBL). Seropositiv...
journal_title:Zeitschrift fur Rheumatologie
pub_type: 杂志文章
doi:
更新日期:1984-05-01 00:00:00
abstract::Because it is a systemic disorder, rheumatoid arthritis (RA) is known to predispose affected individuals to other organ manifestations as well as arthritic problems. The serious complications include pericarditis, pulmonary and cutaneous nodules, episcleritis, and rheumatoid vasculitis. Of late, a significantly increa...
journal_title:Zeitschrift fur Rheumatologie
pub_type: 杂志文章,评审
doi:10.1007/s00393-006-0105-9
更新日期:2006-10-01 00:00:00
abstract::A set of early diagnostic (ED) criteria comprising clinical data, ESR, radiological spinal signs, and the risk factor HLA B27 was evaluated after 5 and 10 years in a follow-up of 54 patients with an initial diagnosis of possible ankylosing spondylitis (AS). After 10 years 32 patients (59%) had developed definite AS ac...
journal_title:Zeitschrift fur Rheumatologie
pub_type: 杂志文章
doi:
更新日期:1990-03-01 00:00:00
abstract::Due to the wide range of positive effects and the clear evidence of effectiveness, physical activity is one of the most important treatments for inflammatory rheumatic diseases. Because of the frequent overlap of disease and age-related aspects in older patients, the implementation of the German national physical acti...
journal_title:Zeitschrift fur Rheumatologie
pub_type: 杂志文章,评审
doi:10.1007/s00393-019-0592-0
更新日期:2019-03-01 00:00:00
abstract::The Churg-Strauss syndrome is a disorder characterized by hypereosinophilia and systemic vasculitis occurring in patients with asthma and allergic rhinitis. Only few patients are identified as having this syndrome. The three histological criteria are necrotizing vasculitis, tissue infiltration by eosinophils, and extr...
journal_title:Zeitschrift fur Rheumatologie
pub_type: 杂志文章
doi:
更新日期:1988-11-01 00:00:00
abstract::The evidence-based recommendations on the management of ankylosing spondylitis (AS) have enjoyed a high level of acceptance and circulation in Germany, as well as in other European countries. To make patient participation in the decision-making process regarding their disease easier, as well as to strengthen the physi...
journal_title:Zeitschrift fur Rheumatologie
pub_type: 杂志文章
doi:10.1007/s00393-009-0428-4
更新日期:2010-03-01 00:00:00
abstract::This paper concerns a radiological and postmortem study of an 81-year-old woman with diffuse intervertebral osteochondrosis (T12-L1 severe erosion) in association with generalized osteoarthritis and articular chondrocalcinosis. Varying degrees of cartilage plate flaws with cartilaginous Schmorl's nodes and vertebral p...
journal_title:Zeitschrift fur Rheumatologie
pub_type: 杂志文章
doi:
更新日期:1979-11-01 00:00:00
abstract::We saw two cases of infectious sacroiliitis in young patients. The multi-various clinical symptoms, the predispositions, as well as the alteration of laboratory analyses are described. Further, we discuss problems of early diagnosis, diagnostic measures like x-ray, sonography, bone-scan, computerized tomography scan, ...
journal_title:Zeitschrift fur Rheumatologie
pub_type: 杂志文章
doi:
更新日期:1992-05-01 00:00:00
abstract:BACKGROUND:Since the introduction of biologic treatment in rheumatoid arthritis (RA), disease activity and treatment modalities have changed substantially. The current provision and developments in recent years are analyzed with annual data from the National Database of the Collaborative Arthritis Centers in Germany. ...
journal_title:Zeitschrift fur Rheumatologie
pub_type: 杂志文章,多中心研究
doi:10.1007/s00393-016-0156-5
更新日期:2017-02-01 00:00:00
abstract::The supernatant of peripheral blood monocytes, cultured with autologous sera from patients with rheumatoid arthritis, stimulates healthy human granulocytes to significantly elevated chemiluminescence (p = 0.05) compared with supernatants of monocytes from osteoarthritic patients or healthy volunteers. Cultures without...
journal_title:Zeitschrift fur Rheumatologie
pub_type: 杂志文章
doi:
更新日期:1988-05-01 00:00:00