Abstract:
:Vaccination with native full-length merozoite surface protein 1 (MSP1) or with recombinant C-terminal peptides protects mice against lethal challenge with virulent malaria parasites. To determine whether other regions of MSP1 can also induce protection, Plasmodium yoelii MSP1 was divided into four separate regions. Each was expressed in Escherichia coli as a fusion protein with glutathione S-transferase (GST). The N-terminal fragment began after the cleavage site for the signal sequence and ended in the region comparable to the cleavage site for the C terminus of the 82-kDa peptide of Plasmodium falciparum. This expressed protein was 30 kDa smaller than the predicted peptide. One peptide from the middle region was produced, and the C terminus consisted of a 42-kDa fragment corresponding to the analogous peptide of P. falciparum and a 19-kDa fragment that extended 37 amino acids in the amino-terminal direction beyond the probable cleavage site. To test protection of mice against lethal P. yoelii challenge, three mouse strains (CAF1, BALB/c, and A/J) were vaccinated with each of the four recombinant proteins of MSP1. Mice vaccinated with the C-terminal 19-kDa protein were highly protected (described previously), as were those vaccinated with the 42-kDa protein that contained the 19-kDa fragment. The N-terminally expressed fragment of P. yoelii was not full length because of proteolytic cleavage in E. coli. The GST-82-kDa partial fragments induced some immunity, but the surviving mice still had high parasitemias. Vaccination with the peptide from the middle region of MSP1 gave minimal to no protection. Therefore, in addition to the C-terminal 19- and 42-kDa proteins, the only other fragment to give protection was the 82-kDa protein. The protection induced by the truncated 82-kDa protein was minimal compared with that of the C-terminal fragments.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Tian JH,Kumar S,Kaslow DC,Miller LHdoi
10.1128/IAI.65.8.3032-3036.1997subject
Has Abstractpub_date
1997-08-01 00:00:00pages
3032-6issue
8eissn
0019-9567issn
1098-5522journal_volume
65pub_type
杂志文章abstract::A nasal vaccine, consisting of outer membrane vesicles (OMVs) from group B Neisseria meningitidis, was given to 12 volunteers in the form of nose drops or nasal spray four times at weekly intervals, with a fifth dose 5 months later. Each nasal dose consisted of 250 microg of protein, equivalent to 10 times the intramu...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.4.1334-1341.1998
更新日期:1998-04-01 00:00:00
abstract::Studies were carried out to determine whether treatment of mice with the synthetic adjuvant muramyl dipeptide afforded any resistance to infection with the obligate intracellular protozoan Toxoplasma gondii. Marked resistance to lethal challenge infection was observed in CBA but not C57BL/6 mice pretreated with muramy...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.31.2.716-722.1981
更新日期:1981-02-01 00:00:00
abstract::A soluble hemagglutinin/protease produced by Vibrio cholerae, which has previously been shown to hydrolyze fibronectin and ovomucin and to cleave lactoferrin and the A subunit of the heat-labile enterotoxin of Escherichia coli, appears to be a zinc metalloendopeptidase. Both its hemagglutinative and protease functions...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.42.2.639-644.1983
更新日期:1983-11-01 00:00:00
abstract::Cell-mediated immune (CMI) responses and tumor necrosis factor alpha (TNF-alpha) have been shown to be essential in acquired protection against Cryptococcus neoformans. Induction of a protective anticryptococcal CMI response includes increases in dendritic cells (DC) and activated CD4(+) T cells in draining lymph node...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.1.68-74.2003
更新日期:2003-01-01 00:00:00
abstract::Patients with chronic bronchitis are colonized by and may develop acute bronchopulmonary infection due to nontypable Haemophilus influenzae (NTHI) despite the presence of bactericidal and opsonizing antibody to the infecting organism. To test the hypothesis that secretory immunoglobulin A (IgA) interferes with host de...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.45.1.36-40.1984
更新日期:1984-07-01 00:00:00
abstract::Gastroesophageal reflux (GER) frequently occurs in patients with respiratory disease and is particularly prevalent in patients with cystic fibrosis. GER is a condition in which the duodenogastric contents of the stomach leak into the esophagus, in many cases resulting in aspiration into the respiratory tract. As such,...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00674-13
更新日期:2014-09-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.7.3681-3688.2002
更新日期:2002-07-01 00:00:00
abstract::The effects of phagocytosis-stimulating factor (PSF) derived from polymorphonuclear neutrophils on macrophage functions were studied. PSF enhanced the initial rate of phagocytosis of serum-opsonized zymosan particles by macrophages, whereas it did not affect the phagocytosis of immunoglobulin G-sensitized and inert zy...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.55.8.1762-1766.1987
更新日期:1987-08-01 00:00:00
abstract::Antiserum to a synthetic peptide, CTP3, consisting of residues 50 to 64 of the B subunit of cholera enterotoxin (CT), a sequence which is conserved in heat-labile enterotoxins from Escherichia coli strains of human (H-LT) or porcine (P-LT) origin, cross-reacted with and neutralized all three enterotoxins. It also cros...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.52.2.562-567.1986
更新日期:1986-05-01 00:00:00
abstract::Acinetobacter baumannii is a pathogen of increasing medical importance with a propensity to be multidrug resistant, thereby making treatment challenging. Little is known of virulence traits in A. baumannii. To identify virulence factors and potential drug targets, random transposon (Tn) mutants derived from the A. bau...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00366-10
更新日期:2010-09-01 00:00:00
abstract::During persistence of nonencapsulated Haemophilus influenzae in the respiratory tracts of patients with chronic bronchitis, the major outer membrane proteins (MOMPs) P2 and P5 show antigenic drift. The hypothesis that appearance of antigenic variants is the consequence of antibody-dependent selection was tested in a r...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.3.980-986.1996
更新日期:1996-03-01 00:00:00
abstract::Persistent infection with Borrelia burgdorferi in the presence of a vigorous host immune response has been demonstrated in humans and in animal models of Lyme disease. Long-term persistence of B. burgdorferi was documented recently in our studies of BALB/c and C3H mice infected with cloned and uncloned strains of B. b...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.8.3521-3527.1994
更新日期:1994-08-01 00:00:00
abstract::Cholera is an acute diarrheal disease that is caused by the gram-negative bacterium Vibrio cholerae. The low efficacy of currently available killed-whole-cell vaccines and the reactinogenicity coupled with potential reversion of live vaccines have thus far precluded widespread vaccination for the control of cholera. R...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.12.7695-7702.2001
更新日期:2001-12-01 00:00:00
abstract::In swine, the most common and severe enterotoxigenic Escherichia coli (ETEC) infections are caused by strains that express K88 (F4)(+) fimbriae, heat-labile enterotoxin (LT), heat-stable enterotoxin b (STb), and enteroaggregative E. coli heat-stable toxin 1. Previous studies based on a design that involved enterotoxin...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01743-07
更新日期:2008-07-01 00:00:00
abstract::An array of pro- and anti-inflammatory mediators of the innate immune system was analyzed in stool, urine, and rectal mucosa samples from adults and children with shigellosis to better understand their role in recovery from and in the immunopathogenesis of the disease. Increased concentrations of lactoferrin (Lf), mye...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.6.3620-3629.2000
更新日期:2000-06-01 00:00:00
abstract::Haemophilus influenzae type b (Hib) is a significant pathogen for young children, and three Hib vaccines (named PRP-OMPC, HbOC, and PRP-T) are currently available for young children. Extensive studies of anti-Hib polysaccharide (PS) antibodies (Abs) have shown that the V regions of Abs against the Hib PS comprise a VH...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.11.4219-4223.1995
更新日期:1995-11-01 00:00:00
abstract::Mucins are high-molecular-weight glycoproteins and major constituents of the mucus layer which covers the airway surface. We have studied the interactions between bacteria, mucins, and epithelial cells from the human respiratory tract. Nontypeable strains of Haemophilus influenzae were found to bind to purified airway...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.7.2485-2492.1995
更新日期:1995-07-01 00:00:00
abstract::Studies concerned with the mechanisms responsible for relative resistance or susceptibility of strains of inbred mice to Trypanosoma musculi infections are presented. Treatment with 400 rads of ionizing radiation, silica dust, or trypan blue (reticuloendothelial blocking agents) rendered C3H mice unable to control the...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.6.1647-1655.1989
更新日期:1989-06-01 00:00:00
abstract::We performed ex vivo experiments with Plasmodium falciparum-infected human placentas from primi- and multigravida women from Cameroon. All women, independent of their gravida status, had anti-chondroitin sulfate A (CSA) adhesion antibodies which cross-reacted with heterologous strains, such as FCR3 and Palo-Alto(FUP)1...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:
更新日期:1999-12-01 00:00:00
abstract::Neonatal piglets have been used as models to study human campylobacteriosis and helicobacteriosis. The purpose of this study was to determine the relative pathogenicities, on the basis of the duration of fecal shedding and colonization of tissues, of three Arcobacter species in 1-day-old cesarean-derived colostrum-dep...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.6.2295-2299.1996
更新日期:1996-06-01 00:00:00
abstract::Evidence that the bactericidal ability and the stimulated oxidative metabolism of leukocytes appear in parallel during fetal development of the Minnesota Miniature pig has been obtained by application of the techniques applied to studies of human cells. It was demonstrated that leukocytes from 87- to 90-day fetuses we...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.5.2.232-237.1972
更新日期:1972-02-01 00:00:00
abstract::Pathogenic leptospires have the ability to survive and disseminate to multiple organs after penetrating the host. Several pathogens, including spirochetes, have been shown to express surface proteins that interact with the extracellular matrix (ECM). This adhesin-mediated binding process seems to be a crucial step in ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00460-06
更新日期:2006-11-01 00:00:00
abstract::To determine optimal strategies to induce specific-antibody-secreting cells (specific ASC) in the rectal and vaginal mucosae, we immunized monkeys with a prototype mucosal immunogen, cholera toxin (CT), given locally or via gastric or parenteral administration. Repeated rectal or vaginal CT immunizations induced stron...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.12.5889-5896.1998
更新日期:1998-12-01 00:00:00
abstract::Sterile sonic-treated materials of pneumococci grown in media containing N-acetylmannosamine as the carbohydrate source were lethal for mice. Purification of the toxic factor led to its identification as the enzyme neuraminidase. ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.2.1.115-117.1970
更新日期:1970-07-01 00:00:00
abstract::Pyolysin (PLO), the hemolytic exotoxin expressed by Arcanobacterium (Actinomyces) pyogenes, is a member of the thiol-activated cytolysin family of bacterial toxins. Insertional inactivation of the plo gene results in loss of expression of PLO with a concomitant loss in hemolytic activity. The plo mutant, PLO-1, has an...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:
更新日期:1999-04-01 00:00:00
abstract::Cells in the Brucella spp. are intracellular pathogens that survive and replicate within host monocytes. Brucella maintains persistent infections in animals despite the production of high levels of anti-Brucella-specific antibodies. To determine the effect of antibody opsonization on the ability of Brucella to establi...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.6.3702-3713.2005
更新日期:2005-06-01 00:00:00
abstract::We investigated the biological properties of a novel staphylococcal enterotoxin (SE)-like toxin type P (SElP). SElP induced a substantial proliferative response and the production of cytokines interleukin-2, gamma interferon, tumor necrosis factor alpha, and interleukin-4 from human T cells when administered at a conc...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.9.5540-5546.2005
更新日期:2005-09-01 00:00:00
abstract::Although Salmonella enterica serovar Typhimurium can undergo phase variation to alternately express two different types of flagellin subunit proteins, FljB or FliC, no biological function for this phenomenon has been described. In this investigation, we constructed phase-locked derivatives of S. enterica serovar Typhi...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.5.3021-3030.2001
更新日期:2001-05-01 00:00:00
abstract::Mice (BALB/cJ, C3H/HeN, and C3H/HeJ) primed with actinomycin D became highly susceptible to lethal intoxication with staphylococcal enterotoxin B (SEB). The mice underwent toxicosis and toxic shock and died. Actinomycin D-primed C3H/HeN and C3H/HeJ mice showed equal sensitivity to SEB, suggesting that bacterial lipopo...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.10.4626-4631.1994
更新日期:1994-10-01 00:00:00
abstract::The macrophage has previously been implicated in contributing to the renal inflammation associated with hemolytic-uremic syndrome (HUS). However, there is currently no in vivo model detailing the contribution of the renal macrophage to the kidney disease associated with HUS. Therefore, renal macrophage recruitment and...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01663-06
更新日期:2007-03-01 00:00:00