Delivery of recombinant tetanus-superoxide dismutase proteins to central nervous system neurons by retrograde axonal transport.

Abstract:

:The nontoxic C fragment of tetanus toxin (TC) can transport other proteins from the circulation to central nervous system (CNS) motor neurons. Increased levels of CuZn superoxide dismutase (SOD) are protective in experimental models of stroke and Parkinson's disease, whereas mutations in SOD can cause motor neuron disease. We have linked TC to SOD and purified the active recombinant proteins in both the TC-SOD and SOD-TC orientations. Light microscopic immunohistochemistry and quantitative enzyme-linked immunosorbant assays (ELISA) of mouse brainstem, after intramuscular injection, demonstrate that the fusion proteins undergo retrograde axonal transport and transsynaptic transfer as efficiently as TC alone.

journal_name

Exp Neurol

journal_title

Experimental neurology

authors

Figueiredo DM,Hallewell RA,Chen LL,Fairweather NF,Dougan G,Savitt JM,Parks DA,Fishman PS

doi

10.1006/exnr.1997.6490

subject

Has Abstract

pub_date

1997-06-01 00:00:00

pages

546-54

issue

2 Pt 1

eissn

0014-4886

issn

1090-2430

pii

S0014-4886(97)96490-6

journal_volume

145

pub_type

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