Abstract:
:The nontoxic C fragment of tetanus toxin (TC) can transport other proteins from the circulation to central nervous system (CNS) motor neurons. Increased levels of CuZn superoxide dismutase (SOD) are protective in experimental models of stroke and Parkinson's disease, whereas mutations in SOD can cause motor neuron disease. We have linked TC to SOD and purified the active recombinant proteins in both the TC-SOD and SOD-TC orientations. Light microscopic immunohistochemistry and quantitative enzyme-linked immunosorbant assays (ELISA) of mouse brainstem, after intramuscular injection, demonstrate that the fusion proteins undergo retrograde axonal transport and transsynaptic transfer as efficiently as TC alone.
journal_name
Exp Neuroljournal_title
Experimental neurologyauthors
Figueiredo DM,Hallewell RA,Chen LL,Fairweather NF,Dougan G,Savitt JM,Parks DA,Fishman PSdoi
10.1006/exnr.1997.6490subject
Has Abstractpub_date
1997-06-01 00:00:00pages
546-54issue
2 Pt 1eissn
0014-4886issn
1090-2430pii
S0014-4886(97)96490-6journal_volume
145pub_type
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journal_title:Experimental neurology
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