Abstract：
OBJECTIVE:To investigate the role of AC-cAMP system in the transmission of the action of the growth hormone releasing hormone (GRH) on growth hormone (GH) release in pituitary GH-secreting adenomas. METHODS:The effects of GRH (10(-7) mol/L) on intracellular cAMP levels and GH release and the effects of AC-cAMP stimulators, cholera toxin (Ct, 50 micrograms/L), forskolin (10(-5) ml/L) and db-cAMP (10(-3) mol/L) on GH secretion were studied in cultured cells of 21 GH-secreting adenomas obtained from operation for acromegalic patients. RESULTS:GRH and Ct failed to stimulate GH secretion in 61.9% (13/21 cases) and 57.1% (12/21 cases) pituitary GH adenoma cell cultures respectively. Forskolin stimulated GH release in 88.9% (8/9 cases), while db-cAMP induced GH secretion in all cases tested (5/5 cases). The intracellular cAMP levels were elevated by GRH in the 4 out of 9 cases of tumor cell cultures, but not in the other 5 cases. According to the GH secretory responses to GRH and Ct, the 21 GH tumors were divided into 4 groups. In group A and B, GRH can stimulate GH release, but Ct has stimulative role only in group A. In group C and D, GRH fails to stimulate GH secretion. However group A can respond to Ct, but group D has no response. CONCLUSIONS:The GH hypersecretion in most acromegalic patients is mainly due to the defects of pituitary adenoma cells, especially the abnormalities of GRH receptor and/or stimulative guanosine protein.
journal_name
Chin Med J (Engl)journal_title
Chinese medical journalauthors
Shi Y,Deng J,Yin Jsubject
Has Abstractpub_date
1996-08-01 00:00:00pages
603-8issue
8eissn
0366-6999issn
2542-5641journal_volume
109pub_type
杂志文章abstract：OBJECTIVE:To explore the methods of lens-sparing (without lensectomy), non-silicone oil tamponade and no scleral buckling for treatment of early giant retinal tears. METHODS:Thirteen cases of early retinal detachment of proliferative vitreoretinopathy (PVR) grade C2-D1, with giant tear extending from 135 degrees to 27...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：1999-03-01 00:00:00
abstract：BACKGROUND:Our previous research has suggested that genes around D12S1056 in 12q13 may confer susceptibility to ventricular septal defect (VSD) in humans. The present study was to define the chromosome region assignment by transmission disequilibrium test (TDT), and to identify the important candidate gene by family-ba...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2006-02-20 00:00:00
abstract：BACKGROUND:The most important objective of transplant studies in the injured spinal cord has been to provide a favorable environment for axonal growth. Moreover, the continuing discovery of new grafts is providing new potentially interesting transplant candidates. Our purpose was to observe the morphological and functi...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2010-09-01 00:00:00
abstract：BACKGROUND:Ophthalmic gel has been developed to increase the drug concentration in aqueous humor and to retard the loss of drug from the conjunctival sac. The research was to compare the drug concentration in aqueous humor of cataract patients administered 0.3% gatifloxacin ophthalmic gel with that in patients administ...
journal_title：Chinese medical journal
pub_type： 杂志文章,随机对照试验
doi：
更新日期：2010-08-05 00:00:00
abstract：:Immunohistochemical and ultrastructural findings in a primary intracranial endodermal sinus tumor are reported in this paper. The tumor cells exhibited AFP, CEA and anti-alpha-1-trypsin positive immunoreactivity immunocytochemically. Aggregates of electron-dense material in the extra- and intracellular spaces and amor...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：1991-06-01 00:00:00
abstract：BACKGROUND:The molecular mechanisms underlying the endometriosis are still not completely understood. In order to test the hypothesis that the approaches in phosphoproteomics might contribute to the identification of key biomarkers to assess disease pathogenesis and drug targets, we carried out a phosphoproteomics anal...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：10.4103/0366-6999.166022
更新日期：2015-10-05 00:00:00
abstract：BACKGROUND:The Dynamic Interspinous Assisted Motion (DIAM) system was designed to stabilize degenerative spinal segments without fusion surgery, maintain segment motion and prevent adjacent segment degeneration. The aim of this study was to investigate clinical efficacy of the DIAM system in treatment of degenerative l...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2010-11-01 00:00:00
abstract：OBJECTIVE:To review the relationship between personal behavior and cultural factors and reproductive tract infections (RTIs). METHODS:An English-language literature search using MEDLINE (1994-1998), Social Science Index (1994-1998), and informal discovery. Any original researches and reviews related to RTIs' epidemiol...
journal_title：Chinese medical journal
pub_type： 杂志文章,评审
doi：
更新日期：1999-11-01 00:00:00
abstract：OBJECTIVE:To evaluate the use of oxygen in neonates in the past, present and future. DATA SOURCES:The data are mainly from Pubmed with relevant published articles from the 1940s to the present with some information gathered from web searches. STUDY SELECTION:Studies evaluating the use of oxygen in premature and term ...
journal_title：Chinese medical journal
pub_type： 杂志文章,评审
doi：
更新日期：2010-10-01 00:00:00
abstract：BACKGROUND:Paraquat (PQ; 1, 1'-dimethyl-4, 4'-bipyridinium), a widely used herbicide that is structurally similar to the known dopaminergic neurotoxicant MPTP (1-methyl-1, 2, 3, 6-tetrahydropyridine), has been suggested as a potential etiologic factor for the development of Parkinson's disease (PD). Aging is an accepte...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2005-08-20 00:00:00
abstract：BACKGROUND:Activation of N-methyl-D-aspartate (NMDA) receptors and alpha-amino-3-hydroxy-5-methyl-4-isoxazole-propionic acid (AMPA) receptors play an important role in the neurons death induced by ischemia. The mitigating effect of intravenous anesthetics on ischemic neuron injury is related to their influence on NMDA ...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2006-09-20 00:00:00
abstract：BACKGROUND:The development of new adjuvants for human use has been the focus of attention. This study's aim is to explore the possibility of using nanoparticle Ca nanoparticles (CA) as a vaccine adjuvant of anti-idiotypic antibody NP30 against schistosomiasis and its protective mechanisms. METHODS:Nanoparticle CA-NP30...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2004-01-01 00:00:00
abstract：OBJECTIVE:To review articles aiming to present an overview of the principles, progress, uses and limitations of laser speckle flowgraphy (LSFG) in posterior fundus circulation research. DATA SOURCES:The data used in this review was obtained mainly from the studies reported in PubMed using the key terms "laser speckle"...
journal_title：Chinese medical journal
pub_type： 杂志文章,评审
doi：
更新日期：2011-12-01 00:00:00
abstract：BACKGROUND:Mitochondrial DNA 4977-bp deletion (DeltamtDNA(4977)) was reported in many human neoplasia. However, its biological significance remains to be evaluated and the molecular mechanism needs to be investigated. In this study, we analyzed the frequency of DeltamtDNA(4977) in gastric cancer (GC) cell lines and tis...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2009-02-20 00:00:00
abstract：OBJECTIVE:To study the histopathological changes of relevant internal organs of Macaca mulatta infected with Plasmodium knowlesi (P. knowlesi). METHODS:Histopathological examination of 3 monkeys who died of P. knowlesi infection, 2 P. knowlesi infected monkeys who died of treatment failure with artesunate suppository ...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2001-10-01 00:00:00
abstract：OBJECTIVE:Clinical application of autologous fat grafting (AFG) is quickly expanding. Despite the widely acceptance, long-term survival rate (SR) of AFG remains a question not yet solved. Meanwhile, although rare, severe complications related to AFG including vision loss, stroke even death could be seen in the literatu...
journal_title：Chinese medical journal
pub_type： 杂志文章,评审
doi：10.4103/0366-6999.156142
更新日期：2015-05-05 00:00:00
abstract：: ...
journal_title：Chinese medical journal
pub_type： 社论
doi：10.4103/0366-6999.245275
更新日期：2018-11-20 00:00:00
abstract：BACKGROUND:Some research groups have hypothesized that human rhinoviruses (HRVs) delayed the circulation of the 2009 pandemic influenza A(H1N1) virus (A(H1N1)pdm09) at the beginning of Autumn 2009 in France. This study aimed to evaluate the relationship between HRV and A(H1N1)pdm09 in pediatric patients with influenza-...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2014-01-01 00:00:00
abstract：:Twenty-one patients, under 6 years of age, with root avulsion of the brachial plexus were treated from 1975 to 1987. Among them, 9 had root avulsion of the upper trunk, 2 root avulsion of the lower trunk with middle trunk broken, and 10 root avulsion of the whole brachial plexus. Follow-up of 16 patients showed excell...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：1990-05-01 00:00:00
abstract：OBJECTIVE:To study the clinicopathologic features and immunoprofile of angiomyofibroblastoma (AMF) with emphasis on differential diagnosis. METHODS:Seven vulvar, two vaginal and one perineal angiomyofibroblastomas were evaluated by light microscopy and immunohistochemistry. The immunohistochemical studies were perform...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2000-11-01 00:00:00
abstract：BACKGROUND:Aerosolized amikacin (AA) is a current option for the management of ventilator-associated pneumonia (VAP) caused by multidrug-resistant Gram-negative bacteria (MDR-GNB), as it is reported that AA could increase the alveolar level of the drug without increasing systemic toxicity. This study aimed to evaluate ...
journal_title：Chinese medical journal
pub_type： 杂志文章,随机对照试验
doi：10.4103/0366-6999.205846
更新日期：2017-05-20 00:00:00
abstract：BACKGROUND:Endogenous hydrogen sulfide is a new neuromodulator which takes part in the regulation of central nervous system physiology and diseases. Whether endogenous hydrogen sulfide in the central nervous system regulates cardiovascular activity is not known. In the present study, we observed the hemodynamic changes...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2011-11-01 00:00:00
abstract：OBJECTIVE:To provide appropriate guidelines for treatment of tandem ossification of the posterior longitudinal ligament (OPLL) and flaval ligament (OFL). Data sources Published articles about OPLL and OFL were selected using Medline and Embase electronic databases. Study selection An English literature search from Janu...
journal_title：Chinese medical journal
pub_type： 杂志文章,评审
doi：
更新日期：2009-01-20 00:00:00
abstract：:Of 40 patients with cervical spine tumor treated from 1980 to 1990, 24 had a primary tumor and 16 a metastatic tumor. Thirty-four patients were operated on through anterior and 5 through posterior approach. Preoperative neck pain was relieved and muscular strength of shoulder and arm increased after operation. Total e...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：1992-07-01 00:00:00
abstract：:Lymphocytes from regional lymph nodes of patients with ovarian carcinoma were immortalized by fusing them with a nonsecreting cell line of murine myeloma (Sp2/0-Ag14). The fusion rate was 0-87.5%. By early cloning and recloning, a hybrid cell line, named HMD4, was established. It has stably secreted human IgG for more...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：1990-06-01 00:00:00
abstract：OBJECTIVE:Sepsis remains a leading cause of death in many Intensive Care Units worldwide. Immunosuppression has been a primary focus of sepsis research as a key pathophysiological mechanism. Given the important role of the negative costimulatory molecules programmed cell death-1 (PD-1) and programmed death-ligand 1 (PD...
journal_title：Chinese medical journal
pub_type： 杂志文章,评审
doi：10.4103/0366-6999.204113
更新日期：2017-04-20 00:00:00
abstract：BACKGROUND:Acute rejection is one of the most important factors for prognosis following liver transplantation. With the use of potent immunosuppressants, acute rejection does not always present typical manifestations. Moreover, other complications often occur concomitantly after liver transplantation, which makes early...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2009-06-20 00:00:00
abstract：BACKGROUND:Hospital-acquired pneumonia (HAP) is the most common hospital-acquired infection in China with substantial morbidity and mortality. But no specific risk assessment model has been well validated in patients with HAP. The aim of this study was to investigate the published risk assessment models that could pote...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：10.1097/CM9.0000000000001252
更新日期：2020-12-03 00:00:00
abstract：BACKGROUND:Since early December 2019, the 2019 novel coronavirus disease (COVID-19) has caused pneumonia epidemic in Wuhan, Hubei province of China. This study aimed to investigate the factors affecting the progression of pneumonia in COVID-19 patients. Associated results will be used to evaluate the prognosis and to f...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：10.1097/CM9.0000000000000775
更新日期：2020-05-05 00:00:00
abstract：:Four calcium channel blockers, i.e. nifedipine, verapamil, cinnarizine and tetrandrine are currently available and used widely in treating cardiovascular diseases. To confirm the effects, if any, of calcium channel blockers on cirrhotic patients with portal hypertension, a study was performed on esophageal variceal pr...
journal_title：Chinese medical journal
pub_type： 临床试验,杂志文章,随机对照试验
doi：
更新日期：1995-11-01 00:00:00
