Abstract:
:Some of the most polymorphic short tandem repeats (STRs) have alleles differing in size by as little as one basepair. Since sizing precision with commercially available internal standards ordinarily does not allow safe discrimination at this level, typing is accomplished through comparisons with allelic ladders run on each gel. Here we introduce the use of optimally spaced sequenced alleles as a dedicated internal standard for measurement and typing of hyperpolymorphic STRs. We have constructed such a dedicated internal standard for HUMACTBP2 ([1]; Moos and Gallwitz, EMBO I., 5 (1983) 757-761) typing, including 25 sequenced, ROX labelled HUMACTBP2 alleles spanning the size range for alleles at this locus (233-333 basepairs (bp)). Comparisons of inter-gel runs of selected alleles with this dedicated standard and the commercial GS500 internal standard demonstrate that only the presently described internal standard is suited for direct typing based on fragment length measurements only. Obvious advantages of this typing procedure are that fragment measurements are precise and in accordance with sequenced lengths, and that the typing procedure via an external allelic ladder is avoided. Sequences of the alleles used in the internal standard as well as of selected alleles for allelic ladders in two other hyperpolymorphic AAAG-repeat STRs, D11S554 (Phromchotikul et al., Hum. Mol. Genet., 3 (1991) 21) and HUMAPOA11 (Kimpton et al., PCR Methods Appl., 3 (1993) 13-22) are also presented. To allow fragment length analysis of these two STRs, five D11S554 alleles, spanning from 176 to 225 basepairs, were added to the dedicated internal standard. Performing similar comparisons as for HUMACTBP2, it is demonstrated that even if sizing accuracy is not as good as with HUMACTBP2, typing based on measured fragment size only may be achieved with both other STRs as well. Selecting different colours for the three STRs, triplex electrophoretic runs were performed of a Norwegian population database of 300 unrelated individuals. The probability that two unrelated individuals have the same type in all three STRs is 5 x 10(-7), and the combined paternity exclusion power 99.77%, indicating that this STR selection may represent a good choice for forensic genetic casework.
journal_name
Forensic Sci Intjournal_title
Forensic science internationalauthors
Dupuy BM,Olaisen Bdoi
10.1016/s0379-0738(97)02137-3subject
Has Abstractpub_date
1997-05-05 00:00:00pages
207-27issue
3eissn
0379-0738issn
1872-6283pii
S0379073897021373journal_volume
86pub_type
杂志文章abstract::Analysing and understanding cryptomarkets is essential to become proactive in the fight against the illicit drug trade. Such a research seeks to combine a diversity of indicators related to the virtual (darknet markets) and physical (the traditional "offline" market) aspects of the illicit drug trade to provide inform...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2017.08.026
更新日期:2017-10-01 00:00:00
abstract::At the beginning of 2015, sixty-two capsules containing red-brown crystals seized in a historical city in Brazil were sent to this forensic laboratory for drug testing analysis. The material was identified as being Brephedrone, a new psychoactive substance and a bromine synthetic cathinone that is related to serotonin...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2017.04.007
更新日期:2017-06-01 00:00:00
abstract::Since 1970, traumatomechanics has been a focal point in research at the Institute for Legal Medicine and Traffic Medicine in Heidelberg. Here, the main topics are the understanding of the interrelation between mechanical strain and the resulting degree of injury; at the forefront of all interest is the determination o...
journal_title:Forensic science international
pub_type: 历史文章,杂志文章,评审
doi:10.1016/j.forsciint.2004.04.082
更新日期:2004-09-10 00:00:00
abstract::Pyrethroids, organic compounds similar to natural pyrethrums, constitute the majority of insecticides. Pyrethroids are widely used around the world owing to their excellent selective toxicity to certain insects. In addition, they are easily found in daily life, accounting for most household pesticides. Owing to the ea...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2019.06.004
更新日期:2019-09-01 00:00:00
abstract::The aim of the study was to describe victim, assailant, assault characteristics for sexual assault victims according to the time between the last sexual assault and the examination, and to provide descriptive data on medico-legal findings. The study was based on 418 examined victims of sexual assault during the year 1...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/s0379-0738(02)00427-9
更新日期:2003-01-28 00:00:00
abstract::In this study, we resolved the allelic distribution of three Y chromosomal short tandem repeats (STRs), DYS19 (n=218), DYS390 (n=257) and DYS393 (n=215), in a population study of 257 unrelated men living in the area of South Wüerttemberg/Hohenzollern. In DYS19 and in DYS393, five different alleles were discovered, and...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/s0379-0738(00)00195-x
更新日期:2000-09-11 00:00:00
abstract::We report a possibly first forensic autopsy case of death following a spontaneous recovery from cardiopulmonary arrest (CPA) after clinical declaration of death: 'Lazarus phenomenon'. A 65-year-old male with congenital deafness and dumbness was found unconscious in his room at a public home. During pre-hospital and cl...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/s0379-0738(02)00107-x
更新日期:2002-06-25 00:00:00
abstract::The adhesion molecules identified in recent years can help improve the diagnosis of the wound age, especially of injuries with a short survival time. This is also indicative of the vitality of the wounds. The material investigated in the study originated from 465 skin wounds. The samples were taken from human autopsy ...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/s0379-0738(00)00258-9
更新日期:2000-09-11 00:00:00
abstract::Forensic pathologists are often involved in the identification of unknown bodies. It is imperative, therefore, that they become aware of a national computerized identification system (NCIC) administered by the FBI. There is also a state system in California which has resulted in the identification of 59 individuals. T...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/0379-0738(86)90013-7
更新日期:1986-02-01 00:00:00
abstract::This study of recreational deaths in the "land of sun and fun" has pointed out several useful factors. Firstly, accidental deaths are more common than naturals. Secondly, the most common accidental death is drowning while swimming. The latter is not usually at public facilities, but rather at unsupervised uninstitutio...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/0379-0738(84)90020-3
更新日期:1984-06-01 00:00:00
abstract::The bodies of socially isolated people may remain undiscovered in their own houses for prolonged periods. Occasionally the body is in situ for sufficient time to become skeletonised, or partially so. Medico-legal investigation of these cases is complicated by degradation and contamination of evidence. Thus, a multidis...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2005.02.016
更新日期:2005-07-16 00:00:00
abstract::Recently, modern cross-sectional imaging techniques such as multi-detector computed tomography (MDCT) have pioneered post mortem investigations, especially in forensic medicine. Such approaches can also be used to investigate bones non-invasively for anthropological purposes. Long bones are often examined in forensic ...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2012.10.029
更新日期:2013-02-10 00:00:00
abstract::The aim of any forensic inquiry concerning accidental or criminal cremations is to identify the victims. This identification depends on an essential approach: a careful collection and ultrasonic cleaning of the fragments and splinters of burned bones, and reconstruction--as far as possible--of bone specimens, which im...
journal_title:Forensic science international
pub_type: 杂志文章,评审
doi:10.1016/s0379-0738(98)00115-7
更新日期:1998-09-28 00:00:00
abstract::A collaborative exercise was carried out by the European DNA Profiling Group (EDNAP) in order to evaluate the distribution of mitochondrial DNA (mtDNA) heteroplasmy amongst the hairs of an individual who displays point heteroplasmy in blood and buccal cells. A second aim of the exercise was to study reproducibility of...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/S0379-0738(03)00181-6
更新日期:2004-02-10 00:00:00
abstract::Sharp force trauma (SFT) in bone and cartilage has been studied extensively. This literature review summarizes knife and saw mark research. Researchers have documented several features of cut surfaces and successfully associated them with various tool characteristics. Most study designs are based on light microscopic ...
journal_title:Forensic science international
pub_type: 杂志文章,评审
doi:10.1016/j.forsciint.2019.03.035
更新日期:2019-06-01 00:00:00
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journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2018.11.006
更新日期:2019-02-01 00:00:00
abstract::There has recently been an increase in the research and implementation of advanced measurement techniques to ballistic toolmark identification. This has led to a shift from greyscale imaging to the acquisition of dense areal datasets. With the addition of mathematical correlation algorithms, these advanced techniques ...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2019.02.054
更新日期:2019-05-01 00:00:00
abstract::A large-scale study of facial soft tissue depths of Caucasian adults was conducted. Over a 2-years period, 967 Caucasian subjects of both sexes, varying age and varying body mass index (BMI) were studied. A user-friendly and mobile ultrasound-based system was used to measure, in about 20min per subject, the soft tissu...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2006.02.034
更新日期:2006-05-15 00:00:00
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journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/s0379-0738(02)00451-6
更新日期:2003-03-12 00:00:00
abstract::Methylphenidate (MPH) is a phenethylamine derivative used in the treatment of childhood attention-deficit hyperactivity disorder. MPH is biotransformed in the body by the hydrolysis of the methyl ester linkage to its metabolite, ritalinic acid. Whereas both compounds are usually measured in plasma and urine, prelimina...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2007.06.024
更新日期:2008-03-21 00:00:00
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journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2011.12.007
更新日期:2012-06-10 00:00:00
abstract::On Sunday 28 April 1996 a lone gunman killed and injured many people at the historic penal settlement ruins at Port Arthur in South Eastern Tasmania, Australia. Thirty-two victims were shot dead and 19 were injured in a short time inside a cafe and along the roadway leading to the site entrance. The gunman then took o...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/s0379-0738(02)00372-9
更新日期:2002-12-04 00:00:00
abstract::A collaborative study on Raman spectroscopy and microspectrophotometry (MSP) was carried out by members of the ENFSI (European Network of Forensic Science Institutes) European Fibres Group (EFG) on different dyed cotton fabrics. The detection limits of the two methods were tested on two cotton sets with a dye concentr...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2012.05.025
更新日期:2012-10-10 00:00:00
abstract::We report about a fatal cytisine intoxication in a 20-year-old man who, according to his mother, had drunken tea prepared from plant material of Laburnum anagyroides with the toxic pyridine-like alkaloid as ingredient, which exhibits pharmacological effects similar to nicotine. Using a liquid chromatographic-mass spec...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2009.01.011
更新日期:2009-04-15 00:00:00
abstract::Laboratory investigations were conducted to evaluate the effect of ultraviolet radiation components and solar radiation exposure as a function of time on the degradation of whole human blood DNA from the standpoint of forensic analysis. Ten μL of whole human male blood samples were exposed to UV-A, UV-B, UV-C, and sol...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2020.110674
更新日期:2021-02-01 00:00:00
abstract::To assess the species composition of necrophagous blow flies (Diptera: Calliphoridae) in the area around the federal city of Switzerland, Bern, sampling with baited bottle traps was performed over the course of 34 weeks in 2014. Six locations ranging from urban to forest habitats were sampled weekly or rather biweekly...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2018.05.022
更新日期:2018-08-01 00:00:00
abstract::Clinical and research-based tests in molecular biology require a substantial amount of DNA and RNA, unfortunately, a considerable number of cells is needed for this amount of sample. Blood is one of the best and easiest source of cells, but is not used due to its invasive drawing methods and the needed volume. Another...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2019.109931
更新日期:2019-10-01 00:00:00
abstract::The survival of the tiger (Panthera tigris) is seriously threatened by poaching to provide raw materials for traditional Chinese medicines (TCMs). Most highly prized are the tiger's bones, which are used in combination with other animal and plant derivatives in pills and plasters for the treatment of rheumatism and ot...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/s0379-0738(02)00045-2
更新日期:2002-04-18 00:00:00
abstract::A new rapid and sensitive high-performance liquid chromatography (HPLC) method has been developed for the simultaneous identification and quantification in human plasma of the 13 most commonly prescribed beta-blockers and one active metabolite-atenolol, sotalol, diacetolol, carteolol, nadolol, pindolol, acebutolol, me...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2003.12.008
更新日期:2004-04-20 00:00:00
abstract::The precision of death time estimation by vitreous potassium using 5 different formulas is compared on a random sample of 600 cases. Mean differences, standard deviations and 95% limits of confidence between real and extrapolated time since death were calculated. The best results were obtained using equations with "me...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2016.10.005
更新日期:2016-12-01 00:00:00