Abstract:
:The insulin-like growth factor II (IGF-II)/mannose-6-phosphate (M-6-P) receptor is known to participate in endocytosis as well as sorting of lysosomal enzymes and is involved in membrane trafficking through rapid cycling between cytosolic membrane compartments and the plasma membrane. Here we demonstrate that IGF-II, acting through the IGF-II/M-6-P receptor, promotes exocytosis of insulin in the pancreatic beta cell. The effect of IGF-II was evoked at nonstimulatory concentrations of glucose, was mediated by a pertussis toxin sensitive GTP-binding protein, was dependent on protein kinase C-induced phosphorylation, and was independent of changes in cytoplasmic free Ca2+ concentration. Since the applied concentration of IGF-II is within the range normally found free in circulation in humans, this novel signaling pathway for the IGF-II/M-6-P receptor is likely to be involved in modulation of insulin exocytosis under physiological conditions.
journal_name
Proc Natl Acad Sci U S Aauthors
Zhang Q,Tally M,Larsson O,Kennedy RT,Huang L,Hall K,Berggren POdoi
10.1073/pnas.94.12.6232subject
Has Abstractpub_date
1997-06-10 00:00:00pages
6232-7issue
12eissn
0027-8424issn
1091-6490journal_volume
94pub_type
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