A technique for standardization and quality control of subjective sperm motility assessments in semen analysis.

Abstract:

OBJECTIVE:To establish a quality control method to monitor and eventually to standardize the subjective assessment of sperm motility in conventional semen analysis. DESIGN:Quality control study running over 2 years. SETTING:University infertility clinic and andrology laboratory. PATIENT(S):Randomly chosen patients attending the clinic. MAIN OUTCOME MEASURE(S):Conventional semen analysis with sperm motility assessed by grading according to the World Health Organization (WHO) criteria and analysis of individual sperm tracks with a computer-aided sperm analysis (CASA) system. Formulas were established to identify, from the track data, the threshold velocity values for distinguishing between motility grades a and b and motility grades b and c for each technician. RESULT(S):The subjective thresholds above which technicians categorized sperm as WHO grades a and b were determined by CASA to be 61 +/- 1 and 11 +/- 1 micron/s (straight-line velocity), respectively. Agreement among three to five technicians over 2 years was reasonable (coefficient of variation < 20%), but threshold values were variable. CONCLUSION(S):Agreement within and between laboratories in the assessment of sperm motility grades could be achieved by agreeing on designated values for threshold velocities for grade a and b sperm. On the basis of such values, CASA analysis could be used to provide the expected percentages of grades a, b, and c forms for quality control samples recorded and distributed on videotapes, against which technicians could adjust their subjective assessments.

journal_name

Fertil Steril

journal_title

Fertility and sterility

authors

Yeung CH,Cooper TG,Nieschlag E

doi

10.1016/s0015-0282(97)81455-0

subject

Has Abstract

pub_date

1997-06-01 00:00:00

pages

1156-8

issue

6

eissn

0015-0282

issn

1556-5653

pii

S0015028297814550

journal_volume

67

pub_type

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