Modification of protein surface hydrophobicity and methionine oxidation by oxidative systems.

abstract：:In this study we sought to better understand the role of the glycoprotein quality control machinery in the assembly of MHC class I molecules with high-affinity peptides. The lectin-like chaperone calreticulin (CRT) and the thiol oxidoreductase ERp57 participate in the final step of this process as part of the peptide-...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wearsch PA,Peaper DR,Cresswell P

更新日期：2011-03-22 00:00:00

abstract：:A human metallothionein (MT) gene was inserted into a bovine papillomavirus (BPV) vector. The chimeric vector (pMTII-BPV) transforms rodent fibroblasts to a cadmium-resistant phenotype. The resistance is due to the high level of expression of human MT-II in those cells. The vector is maintained in the cells as a free ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Karin M,Cathala G,Nguyen-Huu MC

更新日期：1983-07-01 00:00:00

abstract：:A pluripotent cell line, C3H10T1/2, is induced to undergo adipogenesis by a mixture of factors that includes a glucocorticoid such as dexamethasone. We found that expression of myostatin (MSTN), a TGF-beta family member extensively studied in muscle, was induced by dexamethasone under those differentiation conditions....

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Feldman BJ,Streeper RS,Farese RV Jr,Yamamoto KR

更新日期：2006-10-17 00:00:00

abstract：:Selected aspects of the evolutionary process and more specifically of the genetic variation are considered, with an emphasis in studies performed by my group. One key aspect of evolution seems to be the concomitant occurrence of dichotomic, contradictory (dialect) processes. Genetic variation is structured, and the dy...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Salzano FM

更新日期：2000-05-09 00:00:00

abstract：:Most evidence supports the view that ER alpha is responsible for estrogen (ovarian estradiol, E(2))-induced proliferation in the epithelial cells of the mammary gland, but despite this, proliferating epithelial cells do not express ER alpha. We have examined this apparent paradox by studying the role of ER alpha and E...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cheng G,Weihua Z,Warner M,Gustafsson JA

更新日期：2004-03-16 00:00:00

abstract：:Sixteen hybridomas have been identified that secrete antibodies specific to horizontal cells in the carp retina. The hybridomas have been classified into three groups based on their antibody staining patterns: group I, staining associated with all horizontal cells; group II, staining associated with the most abundant ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Young LH,Dowling JE

更新日期：1984-10-01 00:00:00

abstract：:Bruton tyrosine kinase (EC 2.7.1.112) [Btk, encoded by Btk in mice and BTK in humans (formerly known as atk, BPK, or emb)], which is variously mutated in chromosome X-linked agammaglobulinemia patients and X-linked immunodeficient (xid) mice, has the pleckstrin homology (PH) domain at its amino terminus. The PH domain...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yao L,Kawakami Y,Kawakami T

更新日期：1994-09-13 00:00:00

abstract：:The relative importance of local ecological and larger-scale historical processes in causing differences in species richness across the globe remains keenly debated. To gain insight into these questions, we investigated the assembly of plant diversity in the Cerrado in South America, the world's most species-rich trop...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Simon MF,Grether R,de Queiroz LP,Skema C,Pennington RT,Hughes CE

更新日期：2009-12-01 00:00:00

abstract：:Aortic endothelial cells from normal pigs and pigs with von Willebrand disease have been established in long-term cultures. Both cultures appeared similar in terms of general growth characteristics, morphologic features and ultrastructure. Immunofluorescent staining of these cultures with chicken (or rabbit) antiporci...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Booyse FM,Quarfoot AJ,Bell S,Fass DN,Lewis JC,Mann KG,Bowie EJ

更新日期：1977-12-01 00:00:00

abstract：:Albumin Redhill is an electrophoretically slow genetic variant of human serum albumin that does not bind 63Ni2+ and has a molecular mass 2.5 kDa higher than normal albumin. Its inability to bind Ni2+ was explained by the finding of an additional residue of Arg at position -1. This did not explain the molecular basis o...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Brennan SO,Myles T,Peach RJ,Donaldson D,George PM

更新日期：1990-01-01 00:00:00

abstract：:In our 3D NOESY-[(1)H,(15)N,(1)H]-ZQ-TROSY experiment, the TROSY principle (transverse relaxation-optimized spectroscopy) is used in three-dimensional (3D) (15)N-resolved nuclear Overhauser enhancement spectroscopy (NOESY), which enables resonance assignments by sequential nuclear Overhauser effects and the collection...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pervushin KV,Wider G,Riek R,Wüthrich K

更新日期：1999-08-17 00:00:00

abstract：:Site-directed mutagenesis of selected residues of mammalian protein phosphatase 1 (PP-1) has been carried out to further define the mechanism of catalysis, activation by divalent cations, and inhibition by toxins and inhibitory proteins. Mutation of active site residues predicted to bind metals (N124D and H248N) resul...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Huang HB,Horiuchi A,Goldberg J,Greengard P,Nairn AC

更新日期：1997-04-15 00:00:00

abstract：:Poly([14C]adenosine diphosphate ribose) was synthesized from [14C]NAD+ with calf thymus nuclei. The fraction containing poly(adenosine diphosphate ribose) eluted with 0.22--0.40 M phosphate buffer (pH 6.8) from a hydroxylapatite column, was completely hydrolyzed with venom phosphodiesterase, and was separated by DEAE-...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Miwa M,Saikawa N,Yamaizumi Z,Nishimura S,Sugimura T

更新日期：1979-02-01 00:00:00

abstract：:An antibody (anti-p21ser) was raised against a ras p21-related synthetic peptide and was able to recognize specifically the substitution of serine for glycine at amino acid 12 of p21. This substitution causes oncogenic activation of p21. Anti-p21ser was found to immunoprecipitate v-Ki-ras p21 and to strongly inhibit i...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Clark R,Wong G,Arnheim N,Nitecki D,McCormick F

更新日期：1985-08-01 00:00:00

abstract：:We present an adaptation of the hydroxyl radical DNA "footprinting" technique that permits high-resolution mapping of protected regions of RNA. Hydroxyl radical cleaves RNA independently of base sequence and secondary structure of the RNAs examined and allows resolution of protected regions at the single nucleotide le...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wang XD,Padgett RA

更新日期：1989-10-01 00:00:00

abstract：:Genetic manipulation of the alpha(2A)-adrenergic receptor (alpha(2A)-AR) in mice has revealed the role of this subtype in numerous responses, including agonist-induced hypotension and sedation. Unexpectedly, alpha(2)-agonist treatment of mice heterozygous for the alpha(2A)-AR (alpha(2A)-AR(+/-)) lowers blood pressure ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tan CM,Wilson MH,MacMillan LB,Kobilka BK,Limbird LE

更新日期：2002-09-17 00:00:00

abstract：:Mutations of inversin cause type II nephronophthisis, an infantile autosomal recessive disease characterized by cystic kidney disease and developmental defects. Inversin regulates Wnt signaling and is required for convergent extension movements during early embryogenesis. We now show that Inversin is essential for Xen...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lienkamp S,Ganner A,Boehlke C,Schmidt T,Arnold SJ,Schäfer T,Romaker D,Schuler J,Hoff S,Powelske C,Eifler A,Krönig C,Bullerkotte A,Nitschke R,Kuehn EW,Kim E,Burkhardt H,Brox T,Ronneberger O,Gloy J,Walz G

更新日期：2010-11-23 00:00:00

abstract：:We have previously described an antigen-specific I-Ad-restricted T-cell hybridoma, A1.1, that constitutively releases an antigen-specific immunoregulatory activity into supernatants. Using retrovirally mediated gene transfer, we have found that transfer of the T-cell receptor alpha chain (TCR alpha) gene from A1.1 to ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Green DR,Bissonnette R,Zheng HG,Onda T,Echeverri F,Mogil RJ,Steele JK,Voralia M,Fotedar A

更新日期：1991-10-01 00:00:00

abstract：:We examined human immunodeficiency virus (HIV) production in cultured mononuclear cells from 36 seropositive homosexual males. Production was detected by using an HIV p24 antigen ELISA assay in blood mononuclear cells in 54% of asymptomatic, 71% of acquired immunodeficiency syndrome (AIDS)-related complex, and 100% of...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： McElrath MJ,Pruett JE,Cohn ZA

更新日期：1989-01-01 00:00:00

abstract：:Monoclonal antibodies can block cellular interactions that negatively regulate T-cell immune responses, such as CD80/CTLA-4 and PD-1/PD1-L, amplifying preexisting immunity and thereby evoking antitumor immune responses. Ibrutinib, an approved therapy for B-cell malignancies, is a covalent inhibitor of BTK, a member of...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sagiv-Barfi I,Kohrt HE,Czerwinski DK,Ng PP,Chang BY,Levy R

更新日期：2015-03-03 00:00:00

abstract：:The structure of cellulose microfibrils in wood is not known in detail, despite the abundance of cellulose in woody biomass and its importance for biology, energy, and engineering. The structure of the microfibrils of spruce wood cellulose was investigated using a range of spectroscopic methods coupled to small-angle ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fernandes AN,Thomas LH,Altaner CM,Callow P,Forsyth VT,Apperley DC,Kennedy CJ,Jarvis MC

更新日期：2011-11-22 00:00:00

abstract：:The role of ornithine decarboxylase (OrnDCase, EC 4.1.1.17) and of the polyamines [putrescine (Put), spermidine (Spd), and spermine (Spm)] in mouse skin tumor promotion was investigated by the use of alpha-difluoromethylornithine (CHF2-Orn), an enzyme-activated irreversible inhibitor of OrnDCase. 12-O-Tetradecanoylpho...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Weeks CE,Herrmann AL,Nelson FR,Slaga TJ

更新日期：1982-10-01 00:00:00

abstract：:Peptide nucleic acid (PNA) is a DNA mimic that has shown considerable promise as a lead compound for developing gene therapeutic drugs. We report that PNAs targeted to functional and accessible sites in ribosomal RNA can inhibit translation in an Escherichia coli cell-free transcription/translation system, with 50% re...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Good L,Nielsen PE

更新日期：1998-03-03 00:00:00

abstract：:Band 3, the major intrinsic protein of the human erythrocyte membrane, was specifically labeled with the covalent fluorescent probe eosin isothiocyanate. The lateral mobility of labeled band 3 in the plane of the membrane under various conditions of ionic strength and temperature was examined by using the fluorescence...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Golan DE,Veatch W

更新日期：1980-05-01 00:00:00

abstract：:Neural network function requires an appropriate balance of excitation and inhibition to be maintained by homeostatic plasticity. However, little is known about homeostatic mechanisms in the intact central brain in vivo. Here, we study homeostatic plasticity in the Drosophila mushroom body, where Kenyon cells receive f...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Apostolopoulou AA,Lin AC

更新日期：2020-07-14 00:00:00

abstract：:Organic hydroperoxide resistance (Ohr) enzymes are unique Cys-based, lipoyl-dependent peroxidases. Here, we investigated the involvement of Ohr in bacterial responses toward distinct hydroperoxides. In silico results indicated that fatty acid (but not cholesterol) hydroperoxides docked well into the active site of Ohr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Alegria TG,Meireles DA,Cussiol JR,Hugo M,Trujillo M,de Oliveira MA,Miyamoto S,Queiroz RF,Valadares NF,Garratt RC,Radi R,Di Mascio P,Augusto O,Netto LE

更新日期：2017-01-10 00:00:00

abstract：:von Willebrand factor (vWF) is a multimeric glycoprotein that mediates the adhesion of platelets to the subendothelium by binding to platelet glycoprotein Ib. For human vWF, this interaction can be induced in vitro by the antibiotic ristocetin or the snake venom protein botrocetin. A missense mutation, Gly-561-->Ser, ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rabinowitz I,Tuley EA,Mancuso DJ,Randi AM,Firkin BG,Howard MA,Sadler JE

更新日期：1992-10-15 00:00:00

abstract：:In vivo, G protein-coupled receptors (GPCR) for neurotransmitters undergo complex intracellular trafficking that contribute to regulate their abundance at the cell surface. Here, we report a previously undescribed alteration in the subcellular localization of D1 dopamine receptor (D1R) that occurs in vivo in striatal ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dumartin B,Jaber M,Gonon F,Caron MG,Giros B,Bloch B

更新日期：2000-02-15 00:00:00

abstract：:The nuclear exosome-targeting (NEXT) complex functions as an RNA exosome cofactor and is involved in surveillance and turnover of aberrant transcripts and noncoding RNAs. NEXT is a ternary complex composed of the RNA-binding protein RBM7, the scaffold zinc-knuckle protein ZCCHC8, and the helicase MTR4. While RNA inter...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Puno MR,Lima CD

更新日期：2018-06-12 00:00:00

abstract：:A study was made of the effects of di- and trivalent cations on homomeric rho 1-type gamma-aminobutyrate (GABA rho 1) receptors expressed in Xenopus oocytes after injection of mRNA coding for the GABA rho 1 subunit. GABA elicited large currents with a Kd approximately 1 microM. The properties of these GABA rho 1 recep...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Calvo DJ,Vazquez AE,Miledi R

更新日期：1994-12-20 00:00:00