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A new approach to labeling cells with technetium-99m, Part II. Evaluation of radiolabeled human-lymphocyte immune functions.

Abstract：

:Technetium-99m-modified polylysine (MPL) is a mild and efficient method for cell labeling that is easily applicable to human lymphocytes. 99mTc-MPL uptake is maximal in 40 min at room temperature. Cell labeling efficiency (from 60 to 80%) increases with rising concentrations of cells and labeling agent. In vitro stability of 99mTc-MPL on lymphocytes incubated in serum at 37 degrees C is high. Microautoradiography indicates that 99mTc-MPL is equally distributed within the cells. The radiolabeling procedure does not alter expression of surface receptors involved in T lymphocyte effector functions, adhesion function, cytolytic activity or proliferative response to IL-2.

journal_name

Nucl Med Biol

journal_title

Nuclear medicine and biology

authors

Delmon-Moingeon LI,Mahmood A,Davison A,Jones AG

doi

10.1016/0969-8051(95)02011-x

subject

Has Abstract

pub_date

1996-01-01 00:00:00

pages

87-95

issue

1

eissn

0969-8051

issn

1872-9614

pii

0969-8051(95)02011-X

journal_volume

23

pub_type

杂志文章

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