Abstract：
:Effects of vitamin E on cell morphology and cellular protein thiols under oxidative stress was investigated in cultured rat hepatocytes with different vitamin E status. Hepatocytes were incubated in the presence or absence of 100 microM alpha-tocopherol succinate for 24 h then treated with 1.5 mM t-butyl hydroperoxide (t-BH) for different time intervals. Lipid peroxidation, as determined by thiobarbituric acid-reactive substances, was completely inhibited over 60 min of treatment in cells incubated with alpha-tocopherol. The change of cell morphology, as determined by surface blebs formation, was correlated with cellular vitamin E status. Surface blebs were formed in 25.1 +/- 5.2 min in the presence of alpha-tocopherol in contrast to 11.1 +/- 2.9 min in its absence. In cells with alpha-tocopherol, surface blebs were induced even though lipid peroxidation was inhibited. Comparing the depletion of membrane protein thiols with t-BH treatment, twice as many (40%) thiols were lost over 60 min in the absence of alpha-tocopherol whereas 20% were lost in the presence of alpha-tocopherol. In addition, the extent of thiol modification of carbonic anhydrase III, as determined by combining isoelectric focusing analysis with immunoblotting, further demonstrated that alpha-tocopherol helps maintain protein thiols in the reduced state. Results indicate that vitamin E protects cell morphology and prevents the loss of protein thiols with t-BH treatment, and on cell morphology protection is associated with protein thiols rather than membrane lipids.
journal_name
Toxicol Lettjournal_title
Toxicology lettersauthors
Wang ST,Kuo JH,Chou RG,Lii CKdoi
10.1016/s0378-4274(96)03793-9subject
Has Abstractpub_date
1996-12-16 00:00:00pages
91-8issue
2eissn
0378-4274issn
1879-3169pii
S0378-4274(96)03793-9journal_volume
89pub_type
杂志文章abstract：:Malathion was applied to roses in three Finnish greenhouses by hand held lance sprayers. The potential dermal exposure of applicators to this insecticide was measured. Total urine production of each applicator was also collected up to 24 h post application. In the urine samples the specific metabolite of malathion, ma...
journal_title：Toxicology letters
pub_type： 杂志文章
doi：10.1016/s0378-4274(02)00181-9
更新日期：2002-08-05 00:00:00
abstract：:Overexposure to manganese (Mn) has been known to induce neuronal death and neurodegenerative symptoms. However, the precise mechanisms underlying Mn neurotoxicity remain incompletely understood. In the present study, we established a Mn-exposed rat model and found that downregulation of wild type p53-induced phosphata...
journal_title：Toxicology letters
pub_type： 杂志文章
doi：10.1016/j.toxlet.2014.12.019
更新日期：2015-05-19 00:00:00
abstract：:Musk xylene (MX), musk ketone (MK) and musk moskene (MM) are synthetic nitro-containing fragrances. Due to their inherent lipophilicity and environmental persistence, they are frequently detected in environmental samples and especially in aquatic ecosystems. Despite this, the current environmental toxicity database of...
journal_title：Toxicology letters
pub_type： 杂志文章
doi：10.1016/s0378-4274(99)00167-8
更新日期：1999-12-20 00:00:00
abstract：:Human saliva offers a unique noninvasive approach for populational study. Purposes of this study were to investigate the feasibility of using saliva manganese (Mn) concentration as a biomarker of Mn exposure among career welders and to study the variations of Mn, copper (Cu), zinc (Zn), cadmium (Cd), and lead (Pb) in ...
journal_title：Toxicology letters
pub_type： 杂志文章,多中心研究
doi：10.1016/j.toxlet.2007.10.003
更新日期：2008-01-04 00:00:00
abstract：:Letimide is a new efficient analgesic salicylate derivative. In this study we evaluated its cytogenetic and teratogenic potential. For chromosomal aberrations and sister chromatid exchange analysis we tested 250, 375, 500 and 625 micrograms/ml of letimide in human lymphocyte cultures in vitro, and for the in vivo cyto...
journal_title：Toxicology letters
pub_type： 杂志文章
doi：10.1016/0378-4274(91)90095-n
更新日期：1991-04-01 00:00:00
abstract：:Clinical study showed that smoking during pregnancy deceased the thymus size in newborns. However, the long-term effect remains unclear. This study was aimed to observe the effects of prenatal nicotine exposure (PNE) on the development of thymus and the T-lymphocyte subpopulation in mice offspring from the neonatal to...
journal_title：Toxicology letters
pub_type： 杂志文章
doi：10.1016/j.toxlet.2018.12.015
更新日期：2019-04-01 00:00:00
abstract：:Interstitial pulmonary fibrosis is characterized by an abnormal accumulation of fibroblasts with a resultant increase in lung collagen content. Previous research has implied a possible involvement of the T-lymphocyte in this process. We used cyclosporin A (Cy A), a known immunosuppressant, to deplete T-lymphocyte-depe...
journal_title：Toxicology letters
pub_type： 杂志文章
doi：10.1016/0378-4274(85)90162-6
更新日期：1985-08-01 00:00:00
abstract：:Neomycin (an aminoglycoside antibiotic), ethacrynate (a loop diuretic), cisplatin (an anticancer drug) and mercuric chloride are chemically unrelated drugs which present similar ototoxic and nephrotoxic properties. We have found that all these molecules inhibit inositol phosphate turnover induced by carbachol or gluta...
journal_title：Toxicology letters
pub_type： 杂志文章
doi：10.1016/0378-4274(90)90076-x
更新日期：1990-05-01 00:00:00
abstract：:The gut microbiome has tremendous potential to impact health and disease. Various environmental toxicants, including insecticides, have been shown to alter gut microbiome community structures. However, the mechanism that compositionally and functionally regulates gut microbiota remains unclear. Quorum sensing is known...
journal_title：Toxicology letters
pub_type： 杂志文章
doi：10.1016/j.toxlet.2017.10.023
更新日期：2018-02-01 00:00:00
abstract：:A comparison of the effects produced by size-fractionated tire particles (TP10 and TP2.5) and similar-sized urban particulate matter (PM10 and PM2.5), collected in Milan in 2007, on the lungs of mice has been performed. The focus is on early acute lung responses following intratracheal instillation of aerosolized part...
journal_title：Toxicology letters
pub_type： 杂志文章
doi：10.1016/j.toxlet.2010.07.002
更新日期：2010-10-05 00:00:00
abstract：:Both arsenite and benzo(a)pyrene (BaP) are known human carcinogens. Studies on the mode-of-action of arsenite indicate that it can also act as co-carcinogen or as a cancer promoter, and that it can facilitate progression of cancers. Some studies on development of lung cancers have suggested a synergism between arsenit...
journal_title：Toxicology letters
pub_type： 杂志文章
doi：10.1016/j.toxlet.2013.01.004
更新日期：2013-04-12 00:00:00
abstract：:Styrene and its chiral main metabolite styrene-7.8-oxide are well characterized regarding their cytotoxic, genotoxic and neurotoxic properties. To our knowledge, no data exist on the influence of styrene and styrene-7.8-oxide on immune reactions. Epidemiological studies suggest that exposure to environmental pollutant...
journal_title：Toxicology letters
pub_type： 杂志文章
doi：10.1016/j.toxlet.2005.06.014
更新日期：2006-01-05 00:00:00
abstract：:Cultured human proximal tubule cell monolayers maintained on permeable supports were treated simultaneously with the aminoglycoside antibiotic, gentamicin, and poly-L-aspartic acid (PAA), an inhibitor of aminoglycoside nephrotoxicity. Following 4 days of exposure, cell monolayers were placed into Ussing chambers to al...
journal_title：Toxicology letters
pub_type： 杂志文章
doi：10.1016/s0378-4274(96)03858-1
更新日期：1997-02-07 00:00:00
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journal_title：Toxicology letters
pub_type： 杂志文章
doi：10.1016/0378-4274(80)90180-0
更新日期：1980-08-01 00:00:00
abstract：:The intestinal epithelium is the first barrier against food contaminants and is highly sensitive to Fusarium toxins, especially deoxynivalenol (DON) and zearalenone (ZEA). Here, we explored the effects of low doses of DON and/or ZEA in naturally moldy diets on intestinal functions in piglets, including inflammatory re...
journal_title：Toxicology letters
pub_type： 杂志文章
doi：10.1016/j.toxlet.2020.07.032
更新日期：2020-10-15 00:00:00
abstract：:Benzene is a human leukemogen, and a bone marrow toxin and carcinogen in experimental animals. The reactive intermediates involved in benzene toxicity and their mechanism(s) of action have not been clearly delineated. We have investigated the clastogenic and cytotoxic effects of trans,trans-muconaldehyde (MUC), a reac...
journal_title：Toxicology letters
pub_type： 杂志文章
doi：10.1016/s0378-4274(01)00340-x
更新日期：2001-05-19 00:00:00
abstract：:Isolated, perfused and ventilated guinea-pig lungs were exposed for 10 min to acid (sulphuric + nitric acid) aerosol mimicking acid rain at pH 4.5 or 2.5, as well as to a control distilled water aerosol (pH 6.0-6.5). Lung perfusing solution was recovered and thromboxane (TX) B2 and leukotriene (LT) B4 were measured by...
journal_title：Toxicology letters
pub_type： 杂志文章
doi：10.1016/0378-4274(87)90262-1
更新日期：1987-11-01 00:00:00
abstract：:Evaluating myelotoxicity is essential for ensuring the safety of novel drugs before they are approved for clinical applications. Although in vivo prediction of the maximum tolerated doses (MTDs) of anticancer drugs is usually performed in rodents, the results are not always applicable to clinical treatment because dru...
journal_title：Toxicology letters
pub_type： 杂志文章
doi：10.1016/j.toxlet.2017.09.013
更新日期：2017-11-05 00:00:00
abstract：:The effects of vitamin C on acute paracetamol poisoning were studied in mice. Given orally at 50, 100 or 200 mg/kg, either simultaneously or 2 h after the oral administration of 875 mg/kg of paracetamol, vitamin C did not cause any decrease in the mortality rate. In similar conditions in previous experiments methionin...
journal_title：Toxicology letters
pub_type： 杂志文章
doi：10.1016/0378-4274(83)90086-3
更新日期：1983-08-01 00:00:00
abstract：:The clinical use of gentamicin (G) is limited because of its nephrotoxic potential. The administration of G (50 mg/kg) to rats in a 10-day daily treatment gave a biphasic pattern of lactate dehydrogenase (LDH) and N-acetyl-beta-D-glucosaminidase (NAG) excretion. Alkaline phosphatase (ALP) was highly elevated during th...
journal_title：Toxicology letters
pub_type： 杂志文章
doi：10.1016/0378-4274(94)03163-2
更新日期：1995-01-01 00:00:00
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journal_title：Toxicology letters
pub_type： 杂志文章
doi：10.1016/0378-4274(91)90141-r
更新日期：1991-07-01 00:00:00
abstract：:Rhesus monkeys exposed to marijuana smoke either 7 or 2 days/weeks (HI and LO groups, respectively), or ethanol-extracted marijuana smoke for 7 days/week (EM) or sham treatment (SH) for 1 year were sacrificed 7 months following the last exposure. Pulmonary levels of carcinogen-DNA adducts were determined. Although mea...
journal_title：Toxicology letters
pub_type： 杂志文章
doi：10.1016/0378-4274(92)90094-z
更新日期：1992-12-01 00:00:00
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journal_title：Toxicology letters
pub_type： 杂志文章,评审
doi：10.1016/j.toxlet.2017.07.893
更新日期：2017-10-05 00:00:00
abstract：:Inhalation of ultrafine particulate matter (PM) in air pollution increases cardiovascular mortality by passing into systemic circulation and possibly affecting endothelial cell (EC) function. This study identified the chemical constituents, including polycyclic aromatic hydrocarbons (PAHs), in diesel exhaust particula...
journal_title：Toxicology letters
pub_type： 杂志文章
doi：10.1016/j.toxlet.2007.08.014
更新日期：2007-11-01 00:00:00
abstract：:There is experimental and epidemiological evidence demonstrating that polycyclic aromatic hydrocarbons (PAHs) are involved in the pathogenesis of cardiovascular diseases. However, heterocyclic amines (HAs), a class of carcinogenic compounds present in food, which share many biochemical features with PAHs, have not rec...
journal_title：Toxicology letters
pub_type： 杂志文章
doi：10.1016/j.toxlet.2008.11.014
更新日期：2009-03-10 00:00:00
abstract：:Since a number of food and food products contain 2,4-hexadienal (HX), an unsaturated aldehyde, human exposure to HX is likely. Long term HX feeding to rodents induces forestomach cancers. Aldehyde dehydrogenases (ALDH) are key enzymes in the stomach for the metabolism of aldehydes. We examined the effect of short term...
journal_title：Toxicology letters
pub_type： 杂志文章
doi：10.1016/s0378-4274(02)00334-x
更新日期：2003-01-13 00:00:00
abstract：:The upregulated α-synuclein (α-syn) and Tau co-occur in methamphetamine (METH) abusers' brains. Here, we designed experiments mainly to investigate whether α-syn and Tau interact in METH exposure. We detected the expression of α-syn, total Tau, and phosphorylation of Tau at Serine 396 (pSer396 Tau) under in vitro and ...
journal_title：Toxicology letters
pub_type： 杂志文章
doi：10.1016/j.toxlet.2019.11.028
更新日期：2020-02-01 00:00:00
abstract：:Male Swiss OF1 mice received a single oral dose of either 80 mg/kg hexachloro-1,3-butadiene (HCBD) or 80 mg/kg methyl mercury (MeHg). Examination of cryostat kidney sections stained for alkaline phosphatase (APP) revealed damage to about 50% of the proximal tubules after 8 h. Pretreatment with the gamma-glutamyltransp...
journal_title：Toxicology letters
pub_type： 杂志文章
doi：10.1016/0378-4274(90)90017-g
更新日期：1990-02-01 00:00:00
abstract：:Several in vivo and in vitro studies have assessed methods of evaluating the cardio toxicity of compounds during drug development due to its importance for predicting human toxicity. However, in vivo/in vitro relationships have not yet been reported using a zebrafish model. This study determined the bradycardia of 15 ...
journal_title：Toxicology letters
pub_type： 杂志文章
doi：10.1016/j.toxlet.2012.10.018
更新日期：2013-01-10 00:00:00
abstract：:Acrolein, a potent cilioinhibitor of cigarette smoke was tested on synchronously dividing cultures. Mitosis could be blocked whenever the addition of acrolein was made during the cell cycle but this effect was totally reversible (between 0.06 microgram/ml to 1 microgram/ml). Nucleic acid syntheses were more affected t...
journal_title：Toxicology letters
pub_type： 杂志文章
doi：10.1016/0378-4274(82)90043-1
更新日期：1982-12-01 00:00:00
