Abstract:
:We have previously shown that 1-(5-isoquinolinylsulfonyl)-2-methylpiperazine (H7), an inhibitor of protein kinase C, inhibits proliferation of neuroblastoma cells in culture. We have now tested whether the effect of H7 is mediated by MAP kinase and Raf. It is shown that, in Neuro 2a cells, activation of protein kinase C by addition of 4 beta-phorbol-12 beta-myristate-13 alpha-acetate (PMA), leads to phosphorylation of Raf and Mitogen-activated protein kinase (MAP kinase). PMA-induced phosphorylation of these proteins is prevented by H7. When quiescent Neuro 2a were stimulated to proliferate by addition of serum, Raf and MAP kinase were rapidly phosphorylated. Serum-induced phosphorylation of Raf and MAP kinase is prevented by H7. These results suggest that, in Neuro 2a cells, the control of proliferation by protein kinase C could be mediated by phosphorylation (and concomitant activation) of Raf and MAP kinase.
journal_name
Neurosci Lettjournal_title
Neuroscience lettersauthors
Cabedo H,Felipo V,Miñana MD,Grisolía Sdoi
10.1016/0304-3940(96)12867-6subject
Has Abstractpub_date
1996-08-16 00:00:00pages
13-6issue
1eissn
0304-3940issn
1872-7972pii
S0304394096128676journal_volume
214pub_type
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journal_title:Neuroscience letters
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journal_title:Neuroscience letters
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journal_title:Neuroscience letters
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journal_title:Neuroscience letters
pub_type: 杂志文章
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journal_title:Neuroscience letters
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journal_title:Neuroscience letters
pub_type: 杂志文章
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journal_title:Neuroscience letters
pub_type: 杂志文章
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更新日期:2013-03-14 00:00:00
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pub_type: 杂志文章
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journal_title:Neuroscience letters
pub_type: 临床试验,杂志文章
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更新日期:2007-12-11 00:00:00
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journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2006.03.078
更新日期:2006-08-07 00:00:00
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journal_title:Neuroscience letters
pub_type: 杂志文章
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更新日期:1997-04-18 00:00:00
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journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2018.04.025
更新日期:2018-06-11 00:00:00
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journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/s0304-3940(01)01557-9
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pub_type: 杂志文章
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journal_title:Neuroscience letters
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pub_type: 杂志文章
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journal_title:Neuroscience letters
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journal_title:Neuroscience letters
pub_type: 杂志文章
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journal_title:Neuroscience letters
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journal_title:Neuroscience letters
pub_type: 杂志文章
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更新日期:1995-05-05 00:00:00
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journal_title:Neuroscience letters
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