Cell lines expressing an acetylcholine release mechanism; correction of a release-deficient cell by mediatophore transfection.

Abstract:

:Several neuronal and non-neuronal cell lines express a Ca(2+)-dependent mechanism of transmitter release that can be demonstrated after loading the cells with acetylcholine during culture. In contrast, a particular cell line, the neuroblastoma N18TG-2, was found to be deficient for release. We transfected N18TG-2 cells with a plasmid encoding Torpedo mediatophore, a protein able to translocate acetylcholine in response to calcium. The N18TG-2 cells expressed the Torpedo protein which reached their plasma membrane. At the same time, these cells acquired a Ca(2+)-dependent quantal release mechanism similar to the one naturally expressed by other cell lines. Hence, the presence of mediatophore in the plasma membrane seems essential for quantal release.

journal_name

J Neurosci Res

authors

Falk-Vairant J,Meunier FM,Lesbats B,Corrèges P,Eder-Colli L,Salem N,Synguelakis M,Dunant Y,Israël M

doi

10.1002/(SICI)1097-4547(19960801)45:3<195::AID-JNR

subject

Has Abstract

pub_date

1996-08-01 00:00:00

pages

195-201

issue

3

eissn

0360-4012

issn

1097-4547

pii

10.1002/(SICI)1097-4547(19960801)45:3<195::AID-JNR

journal_volume

45

pub_type

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