Abstract:
:Several neuronal and non-neuronal cell lines express a Ca(2+)-dependent mechanism of transmitter release that can be demonstrated after loading the cells with acetylcholine during culture. In contrast, a particular cell line, the neuroblastoma N18TG-2, was found to be deficient for release. We transfected N18TG-2 cells with a plasmid encoding Torpedo mediatophore, a protein able to translocate acetylcholine in response to calcium. The N18TG-2 cells expressed the Torpedo protein which reached their plasma membrane. At the same time, these cells acquired a Ca(2+)-dependent quantal release mechanism similar to the one naturally expressed by other cell lines. Hence, the presence of mediatophore in the plasma membrane seems essential for quantal release.
journal_name
J Neurosci Resjournal_title
Journal of neuroscience researchauthors
Falk-Vairant J,Meunier FM,Lesbats B,Corrèges P,Eder-Colli L,Salem N,Synguelakis M,Dunant Y,Israël Mdoi
10.1002/(SICI)1097-4547(19960801)45:3<195::AID-JNRsubject
Has Abstractpub_date
1996-08-01 00:00:00pages
195-201issue
3eissn
0360-4012issn
1097-4547pii
10.1002/(SICI)1097-4547(19960801)45:3<195::AID-JNRjournal_volume
45pub_type
杂志文章abstract::CD9 is a tetra-membrane-spanning glycoprotein involved in cell adhesion, migration, and proliferation in both the immune and the immature nervous system. In this study, CD9 expression was detected in myelin of mouse brain, starting at postnatal day 16. The amount of CD9 protein continuously increased with age and pers...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4547(19971015)50:2<312::AID-JNR
更新日期:1997-10-15 00:00:00
abstract::Inhibitors of differentiation (Id) play an essential role in the neurogenesis of the central nervous system. However, the expression and function of Id in the development of cochlear sensory epithelial cells have yet to be elucidated. In this study, we demonstrate the Id1 gene was expressed in the rapidly growing otoc...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.21133
更新日期:2007-02-15 00:00:00
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pub_type: 杂志文章
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更新日期:1994-04-01 00:00:00
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doi:10.1002/jnr.10123
更新日期:2002-02-15 00:00:00
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journal_title:Journal of neuroscience research
pub_type: 杂志文章
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更新日期:1995-01-01 00:00:00
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更新日期:2010-09-01 00:00:00
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journal_title:Journal of neuroscience research
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更新日期:2009-07-01 00:00:00
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journal_title:Journal of neuroscience research
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更新日期:1996-09-01 00:00:00
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更新日期:2005-08-15 00:00:00
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journal_title:Journal of neuroscience research
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更新日期:1993-07-01 00:00:00
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journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490080113
更新日期:1982-01-01 00:00:00
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pub_type: 杂志文章
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更新日期:2016-02-01 00:00:00
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更新日期:2004-08-15 00:00:00
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更新日期:2017-01-02 00:00:00
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journal_title:Journal of neuroscience research
pub_type: 杂志文章,评审
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更新日期:2000-01-01 00:00:00
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journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490280418
更新日期:1991-04-01 00:00:00
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journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490010206
更新日期:1975-01-01 00:00:00
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journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:
更新日期:1999-12-01 00:00:00
abstract::An immortalized dorsal root ganglion cell line F-11 exhibits many properties of spinal cord neurons and undergoes apoptosis in response to growth factor withdrawal and the exogenous addition of inhibitors of phosphatidylinositol-3-kinase (PI3K). To elucidate the mechanism of apoptosis we generated F-11 clones which ov...
journal_title:Journal of neuroscience research
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doi:
更新日期:1999-09-15 00:00:00
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更新日期:1979-01-01 00:00:00
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更新日期:2020-05-01 00:00:00
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journal_title:Journal of neuroscience research
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更新日期:2007-06-01 00:00:00
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journal_title:Journal of neuroscience research
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更新日期:1976-01-01 00:00:00
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journal_title:Journal of neuroscience research
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doi:
更新日期:1997-08-15 00:00:00
abstract::Chloride efflux is known to be involved in the progression of apoptosis in various cell types. We have recently shown that the volume-sensitive outwardly rectifying (VSOR) anion channel serves as the pathway for apoptotic chloride efflux in some cells. In the present study, we tested the neuroprotective effects of dru...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
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更新日期:2007-05-15 00:00:00
abstract::Previous studies have shown that the mechanical wounding of 3-week-old cultured rat astrocytes results in cell proliferation and hypertrophy resembling astrocyte responses to a brain injury in vivo. We now report the effects of basic fibroblast growth factor (bFGF) and an anti-bFGF antibody on astrocyte morphology, pr...
journal_title:Journal of neuroscience research
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doi:10.1002/jnr.490400310
更新日期:1995-02-15 00:00:00
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journal_title:Journal of neuroscience research
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更新日期:2001-12-01 00:00:00
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更新日期:1997-12-01 00:00:00
abstract::This Review describes some in vitro approaches used to investigate the mechanisms involved in Krabbe's disease, with particular regard to the cellular systems employed to study processes of inflammation, apoptosis, and angiogenesis. The aim was to update the knowledge on the results obtained from in vitro models of th...
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更新日期:2016-11-01 00:00:00