Sequence-specific binding and cleavage of duplex DNA by a radioiodinated, intercalator-linked, triplex-forming oligonucleotide.

Abstract:

:Applications of oligodeoxynucleotides to modulate gene expression have been the subject of much recent research. We have sought to develop a method to permanently inactivate a gene, or potentially kill cells containing abnormal genes. In this report, we show that a DNA intercalator conjugated to a triplex-forming oligonucleotide can be labeled with an Auger electron emitting radioisotope, can cleave its duplex DNA target, and can specifically bind the target sequence contained in a total of 10 kilobases of irrelevant DNA.

journal_name

Nucl Med Biol

authors

Orson FM,McShan WM,Kinsey BM

doi

10.1016/0969-8051(96)00034-0

subject

Has Abstract

pub_date

1996-05-01 00:00:00

pages

519-24

issue

4

eissn

0969-8051

issn

1872-9614

pii

0969-8051(96)00034-0

journal_volume

23

pub_type

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