Changes in the corneal Na-K ATPase levels in eyes stored in moist chamber at 4 degrees C.

Abstract:

:This report deals with a chronological measurement of Na-K ATPase enzyme activity in human and bovine corneas stored in a moist chamber at 4 degrees C. Paired human and bovine eyes were sterilized by the standard eye bank procedure and stored up to 6 days. At the desired time, the corneal endothelium was assayed for Na-K ATPase activity. The protein content of each tissue sample was also determined. In a parallel set of experiments, the viability of identical stored corneas was determined by trypan blue and alizarin red staining technique, and morphometric analysis was done to quantify the extent of the corneal endothelial damage. The human corneas showed that there was a significant progressive decrease in the Na-K ATPase activity as the storage time increased. The decrease was related to morphological endothelial damage.

journal_name

Indian J Ophthalmol

authors

Devi B,Hasany SM,Basu PK

subject

Has Abstract

pub_date

1996-03-01 00:00:00

pages

15-8

issue

1

eissn

0301-4738

issn

1998-3689

journal_volume

44

pub_type

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