Determination of the 5' and 3' terminal noncoding sequences of the bi-segmented genome of the avibirnavirus infectious bursal disease virus.

Abstract:

:Terminal sequences of the bi-segmented dsRNA genome of 3 different strains of infectious bursal disease virus (IBDV) were analyzed by the rapid amplification of cDNA 5' ends (5'RACE) procedure. Both segments are 85% homologous in a 32-nucleotide sequence comprising the 5' end, whereas the 3' end has a conserved pentamer. Comparison to published terminal sequences of other IBDV strains revealed high conservation between the two segments but more serotype-specific nucleotide changes (5 on segment A and 3 on segment B) in the 5' noncoding region compared to the 3' noncoding region (none on segment A and 1 on segment B).

journal_name

Arch Virol

journal_title

Archives of virology

authors

Kibenge FS,Nagarajan MM,Qian B

doi

10.1007/BF01718616

subject

Has Abstract

pub_date

1996-01-01 00:00:00

pages

1133-41

issue

6

eissn

0304-8608

issn

1432-8798

journal_volume

141

pub_type

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