Abstract：
:The kinetics of fluoresceindiacetate (FDA)-hydrolysis in erythrocytes, CHO-cells and homogenized cells was investigated by means of fluorescence spectroscopy. At a 10(-6)m FDA concentration the enzymatic conversion in the homogenized cells can be described by first order reactions. When studying intact cells, total kinetics requires the addition of a first order transport term. Consequently, the total hydrolytic process can be described by three exponentials. The following model was set up on the basis of the data obtained: FDA, which exists in two sterically differing conformations, reaches the cell via an "active transport mechanism", where the two conformations are converted at different rates into fluorescein by an enzymatic reaction. Intracellular FDA-enrichment factors of 100-6-- were derived. In addition, the action of chemical inhibitors and of elevated temperatures on the individual reaction steps was investigated.
journal_name
Radiat Environ Biophysjournal_title
Radiation and environmental biophysicsauthors
Sontag Wdoi
10.1007/BF01331024subject
Has Abstractpub_date
1977-04-27 00:00:00pages
1-12issue
1eissn
0301-634Xissn
1432-2099journal_volume
14pub_type
杂志文章abstract：:High steady-state transcriptional activity is essential for normal mitochondrial function. The requisite transcription rate is satisfied in part by high copy number of mitochondrial DNA (mtDNA). In the present study, we analyze mtDNA copy number by real-time PCR in nucleated blood cells from control mice and mice expo...
journal_title：Radiation and environmental biophysics
pub_type： 杂志文章
doi：10.1007/s00411-010-0329-6
更新日期：2011-03-01 00:00:00
abstract：:The non-cancer mortality data for cerebrovascular disease (CVD) and cardiovascular diseases from Report 13 on the atomic bomb survivors published by the Radiation Effects Research Foundation were analysed to investigate the dose-response for the influence of radiation on these detrimental health effects. Various param...
journal_title：Radiation and environmental biophysics
pub_type： 杂志文章
doi：10.1007/s00411-012-0410-4
更新日期：2012-05-01 00:00:00
abstract：:The observable responses of living systems to ionizing radiation depend on the level of biological organization studied. Understanding the relationships between the responses characteristic of the different levels of organization is of crucial importance. The main objective of the present study is to investigate how s...
journal_title：Radiation and environmental biophysics
pub_type： 杂志文章
doi：10.1007/s00411-011-0382-9
更新日期：2011-11-01 00:00:00
abstract：:Fluence spectra at several ground distances in Hiroshima and Nagasaki are provided along with associated fluence-to-kerma coefficients from the Dosimetry System 2002 (DS02). Also included are transmission factors for calculating expected responses of in situ sample measurements of neutron activation products such as (...
journal_title：Radiation and environmental biophysics
pub_type： 杂志文章
doi：10.1007/s00411-007-0120-5
更新日期：2007-11-01 00:00:00
abstract：:A Monte Carlo model is presented to study details of the energy deposition inside tracks of heavy charged particles in water vapor. The input data for most of the calculations based on the binary encounter approximation are double-differential cross sections for electron emission after heavy-ion impact. The paths of t...
journal_title：Radiation and environmental biophysics
pub_type： 杂志文章,评审
doi：10.1007/BF01219334
更新日期：1994-01-01 00:00:00
abstract：:The main aim of this study was to investigate the dosimetric characteristics of the INTRABEAM ® system in the presence of air gaps between the surface of applicators (APs) and tumor bed. Additionally, the effect of tissue heterogeneities was another focus. Investigating the dosimetric characteristics of the INTRABEAM®...
journal_title：Radiation and environmental biophysics
pub_type： 杂志文章
doi：10.1007/s00411-020-00835-0
更新日期：2020-05-01 00:00:00
abstract：:The heads of rats were irradiated by 4 MeV electrons in doses 90, 180, and 360 Gy. The observed times of deaths ranged 120-600, 60-420, and 150-370 min after 90, 180, and 360 Gy, respectively. A dose dependent decrease of the brain uptake index of haloperidol was observed 1 and 3 h post radiation. On the other hand an...
journal_title：Radiation and environmental biophysics
pub_type： 杂志文章
doi：10.1007/BF01210512
更新日期：1991-01-01 00:00:00
abstract：:The effects of single pulses and multiple pulses of 7 MV electrons on micronuclei (MN) induction in cytokinesis-blocked human peripheral blood lymphocytes (PBLs) were investigated over a wide range of dose rates per pulse (instantaneous dose rate). PBLs were exposed to graded doses of 2, 3, 4, 6, and 8 Gy of single el...
journal_title：Radiation and environmental biophysics
pub_type： 杂志文章
doi：10.1007/s00411-011-0353-1
更新日期：2011-05-01 00:00:00
abstract：:The main target of this work is to examine blood clearance and external exposure for 177Lu-DOTATATE compared with new emerging 177Lu-PSMA therapy. Blood clearance and radiation exposure of 31 patients treated with 5.5 ± 1.1 GBq 177Lu-DOTATATE were compared to those of 23 patients treated with 7.4 GBq 177Lu-PSMA. Dose ...
journal_title：Radiation and environmental biophysics
pub_type： 杂志文章
doi：10.1007/s00411-017-0721-6
更新日期：2018-03-01 00:00:00
abstract：:Indoor exposure to natural radon is a factor that influences lung cancer risk worldwide. The present study includes a meta-analysis of epidemiological data on the relationship between lung cancer and indoor radon. Altogether, 31 case-control studies with 20,703 cases, 34,518 controls and 140 individual odds ratio (OR)...
journal_title：Radiation and environmental biophysics
pub_type： 杂志文章,meta分析
doi：10.1007/s00411-018-0770-5
更新日期：2019-03-01 00:00:00
abstract：:Dose conversion coefficients (DCCs) for assessment of internal and external radiation exposures to terrestrial and aquatic biota are compiled for 75 radionuclides, for 14 terrestrial and 22 aquatic reference organisms. DCC values for internal exposure are calculated based on a homogeneous distribution of the radionucl...
journal_title：Radiation and environmental biophysics
pub_type： 杂志文章
doi：10.1007/s00411-008-0159-y
更新日期：2008-04-01 00:00:00
abstract：:Within the track structure code PARTRAC, DNA strand break induction by direct and indirect radiation action was calculated for the E. coli catabolite gene activator protein (CAP) DNA complex with (125)I located at the position of the H(5) atom of the cytosine near the center. The shape of the resulting DNA fragment si...
journal_title：Radiation and environmental biophysics
pub_type： 杂志文章
doi：10.1007/s00411-004-0231-1
更新日期：2004-05-01 00:00:00
abstract：:In order to provide fundamental data required for dose evaluation due to environmental exposures, effective dose conversion coefficients, that is, the effective dose rate per unit activity per unit area, were calculated for a number of potentially important radionuclides, assuming an exponential distribution in ground...
journal_title：Radiation and environmental biophysics
pub_type： 杂志文章
doi：10.1007/s00411-012-0432-y
更新日期：2012-11-01 00:00:00
abstract：:Simple, ellipsoidal geometries have long been the standard for estimating radiation dose rates in non-human biota (NHB). With the introduction of a regulatory protection standard that emphasizes protection of NHB as its own end point, there has been interest in improved models for the calculation of dose rates in NHB....
journal_title：Radiation and environmental biophysics
pub_type： 杂志文章
doi：10.1007/s00411-014-0546-5
更新日期：2014-08-01 00:00:00
abstract：:The work reported here was originally motivated by a discussion of Monte Carlo computer codes for electron transport in water given in Report No. 130 by the National Council on Radiation Protection and Measurements (NCRP). It was pointed out (correctly) that a published depth-dose distribution calculated by the Oak Ri...
journal_title：Radiation and environmental biophysics
pub_type： 杂志文章
doi：10.1007/s00411-003-0201-z
更新日期：2003-10-01 00:00:00
abstract：:This work describes the radiological assessment of the near-surface Maisiagala radioactive waste repository (Lithuania) over the period 2005-2012, with focus on water pathways and special emphasis on tritium. The study includes an assessment of the effect of post-closure upgrading, the durability of which is greater t...
journal_title：Radiation and environmental biophysics
pub_type： 杂志文章
doi：10.1007/s00411-013-0459-8
更新日期：2013-05-01 00:00:00
abstract：:Radiological impact assessment for flora and fauna requires adequate dosimetric data. Due to the variability of habitats, shapes, and masses of the non-human biota, assessment of doses is a challenging task. External and internal dose conversion coefficients for photons and electrons have been systematically calculate...
journal_title：Radiation and environmental biophysics
pub_type： 杂志文章
doi：10.1007/s00411-006-0061-4
更新日期：2006-09-01 00:00:00
abstract：:Working from the general postulate that natural selection of plant form operates so as to maximize the survival potential of a species, this paper examines the hypothesis that the mechanical support of tree foliage must approach optimality in the use of wood, i.e., that tree stems and branches will have "optimal form"...
journal_title：Radiation and environmental biophysics
pub_type： 杂志文章
doi：10.1007/BF01323263
更新日期：1978-08-10 00:00:00
abstract：:One of the main purposes leading botanists to investigate the effects of ionizing radiations is to understand plant behaviour in space, where vegetal systems play an important role for nourishment, psychological support and functioning of life support systems. Ground-based experiments have been performed with particle...
journal_title：Radiation and environmental biophysics
pub_type： 杂志文章,评审
doi：10.1007/s00411-010-0343-8
更新日期：2011-03-01 00:00:00
abstract：:The antioxidant properties of hydroxycinnamic acid derivatives (HCA) were studied by laser flash photolysis. The transient species with maximum absorption at 360 nm were assigned to the phenoxyl radical of HCA. The SO (4) (.-) induced oxidation of HCA was also investigated. It was shown that the interaction of SO (4) ...
journal_title：Radiation and environmental biophysics
pub_type： 杂志文章
doi：10.1007/s00411-006-0041-8
更新日期：2006-05-01 00:00:00
abstract：:In spite of a well-known relationship between exposure to radiation and increased risk for cancer development, the biological mechanisms involved in radiation-induced carcinogenesis remain poorly documented. Various hypotheses are discussed in this paper. It appears that radiation cannot be directly responsible for th...
journal_title：Radiation and environmental biophysics
pub_type： 杂志文章,评审
doi：10.1007/BF01209747
更新日期：1995-11-01 00:00:00
abstract：:Ehrlich ascites tumour cells were irradiated with negative pions at different positions in the plateau and the peak of the depth absorbed dose curve. Dose-survival curves for immediate testing of the viability of the cells are given and are compared with other types of radiations in use for therapy. From the data obta...
journal_title：Radiation and environmental biophysics
pub_type： 杂志文章
doi：10.1007/BF01358286
更新日期：1979-08-10 00:00:00
abstract：:Model experiments with two structurally different proteins (alcohol dehydrogenase and salmine) show that glycine, alanine, and tyrosine are by far more frequently involved in photochemically induced cross-link formations with DNA than is cysteine. The yields for cross-link formation of thymidine with salmine (cysteine...
journal_title：Radiation and environmental biophysics
pub_type： 杂志文章
doi：10.1007/BF01332171
更新日期：1976-07-30 00:00:00
abstract：:In mice, persisting radiation-induced growth retardation of hematopoietic tissue suggested that at least part of the surviving stem cells are genetically injured. Additional mitotic stress some time after the radiation insult might remove injured stem cells, thus improving the overall recovery of the irradiated bone m...
journal_title：Radiation and environmental biophysics
pub_type： 杂志文章
doi：10.1007/BF01211410
更新日期：1987-01-01 00:00:00
abstract：:The use of Green's function has played a fundamental role in transport calculations for high-charge high-energy (HZE) ions. Two recent developments have greatly advanced the practical aspects of implementation of these methods. The first was the formulation of a closed-form solution as a multiple fragmentation perturb...
journal_title：Radiation and environmental biophysics
pub_type： 杂志文章
doi：10.1007/BF01211542
更新日期：1995-08-01 00:00:00
abstract：:Bone sarcoma as a second malignancy is rare but highly fatal. The present knowledge about radiation-absorbed organ dose-response is insufficient to predict the risks induced by radiation therapy techniques. The objective of the present study was to assess the treatment-induced risk for bone sarcoma following a childho...
journal_title：Radiation and environmental biophysics
pub_type： 杂志文章
doi：10.1007/s00411-013-0510-9
更新日期：2014-05-01 00:00:00
abstract：:The aim of this study was to verify whether extremely low frequency (ELF) magnetic fields (MF) could affect transposition activity like some environmental stress factors such as heat shock or UV irradiation. Using an Escherichia coli Lac Z(-) strain transformed with a plasmid containing a Tn 10 derivative element expr...
journal_title：Radiation and environmental biophysics
pub_type： 杂志文章
doi：10.1007/s00411-003-0192-9
更新日期：2003-07-01 00:00:00
abstract：:Investigation of cell-free DNA (cf-DNA) in body fluids, as a potential biomarker for assessing the effect of ionizing radiation on the organism, is of considerable interest. We investigated changes in the contents of cell-free mitochondrial DNA (cf-mtDNA) and cell-free nuclear DNA (cf-nDNA) in the urine of X-ray-expos...
journal_title：Radiation and environmental biophysics
pub_type： 杂志文章
doi：10.1007/s00411-015-0599-0
更新日期：2015-08-01 00:00:00
abstract：:Native horseradish peroxidase, as well as its reduced and carboxymethylated form, and the apoenzyme, showed resistance to photodynamic action. Sensitivity to this action was detected only in reduced and carboxymethylated apoenzyme, when the photooxidation of its histidine residues was observed. When analyzing the bulk...
journal_title：Radiation and environmental biophysics
pub_type： 杂志文章
doi：10.1007/BF01212653
更新日期：1985-01-01 00:00:00
abstract：:A number of approaches have been proposed to estimate the exposure of non-human biota to ionizing radiation. This paper reports an inter-comparison of the unweighted absorbed dose rates for the whole organism (compared as dose conversion coefficients, or DCCs) for both internal and external exposure, estimated by 11 o...
journal_title：Radiation and environmental biophysics
pub_type： 杂志文章
doi：10.1007/s00411-007-0124-1
更新日期：2007-11-01 00:00:00
