Abstract:
:Der f2 protein in a highly antigenic form was directly expressed in bacteria. Plasmid pFLU11 derived from pKK233-2 was designed to express methionyl-Der f2 under the control of the trc promoter and it has the replication origin of pUC118 instead of its original to increase the copy number. This expression plasmid directed the synthesis of recombinant Der f2 (rDer f2) protein in an insoluble form of inclusion bodies in Escherichia coli cells. The high copy number plasmid pFLU11 conferred the efficient production of the Der f2 protein in E. coli, when compared to a nonchanged origin material. rDer f2 inclusion bodies were easily solubilized in urea and renatured by dialysis to assume the active form. The rDer f2 protein was purified by means of anion exchange and gel filtration chromatography. This expression system yielded about 10 mg of purified rDer f2 protein from the 1L culture. Purified rDer f2 protein reacted with IgE from patient sera almost identically to the native Der f2 in the RAST enzyme immunoassay and skin prick test.
journal_name
Int Arch Allergy Immunoljournal_title
International archives of allergy and immunologyauthors
Iwamoto N,Nishiyama C,Yasuhara T,Saito A,Yuuki T,Okumura Y,Okudaira Hdoi
10.1159/000237263subject
Has Abstractpub_date
1996-04-01 00:00:00pages
356-61issue
4eissn
1018-2438issn
1423-0097journal_volume
109pub_type
杂志文章abstract:INTRODUCTION:The aim of this study was to evaluate the effectiveness of specific immunotherapy (SIT) management with allergoids in children with allergic asthma by monitoring changes in clinical parameters and inflammatory markers in exhaled breath. METHODS:The study population included 43 patients (24 males) of 6-14 ...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000368832
更新日期:2014-01-01 00:00:00
abstract::We describe an HLA-B8, DR3-positive patient with localized morphea after silicone gel breast implantation for cosmetic reasons. We believe that this case suggests that a genetic background, i.e. HLA-B8, DR3 haplotype, is involved in the autoimmune response to silicone. ...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000237437
更新日期:1997-01-01 00:00:00
abstract::Eosinophils used to be thought of as regulators of allergic inflammation, but there is now evidence to the contrary; eosinophils have been found to be the major effector cells responsible for the late asthmatic response (LAR), airway hyperresponsiveness (AHR) and, at least in part, airway remodeling by releasing leuko...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000053829
更新日期:2001-08-01 00:00:00
abstract:BACKGROUND:Specific immunotherapy for respiratory allergy, a seasonal disease, significantly reduces the inflammatory process, attenuating the clinical symptoms. The mechanism for the clinical beneficial effect of immunotherapy has not yet been clarified. Melatonin shows a circadian and seasonal variation and together ...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000049498
更新日期:2001-09-01 00:00:00
abstract:BACKGROUND:Lactobacillus casei is a nonpathogenic gram-positive bacterium widely used in dairy products and has been shown to enhance the cellular immunity of the host. METHODS:To examine the inhibitory effect of L. casei on IgE production, splenocytes obtained from ovalbumin (OVA)-primed BALB/c mice were restimulated...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000069458
更新日期:1998-04-01 00:00:00
abstract:UNLABELLED:T-bet is crucially implicated in Th1 differentiation due to its strong promoting activity for IFN-gamma gene transcription. However, the regulatory role of T-bet in Th2 cytokines is not fully delineated. METHODS:The effect of T-bet on mRNA expression as well as the promoter activity of IL-4 in human T cells...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000101408
更新日期:2007-01-01 00:00:00
abstract::Contact sensitivity to para-phenylenediamine (PPD) is a frequent delayed-type hypersensitivity resulting in contact dermatitis. The aim of the present study, conducted in 16 patients allergic to PPD (as assessed by a positive patch test), was to get better insight into the mechanism of T-cell activation in PPD contact...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000236573
更新日期:1993-01-01 00:00:00
abstract:BACKGROUND:Immunoglobulin G4-related disease (IgG4-RD) is a systemic immunological disorder characterized by fibro-inflammatory conditions; however, the pathobiology of IgG4-RD has not been fully identified. OBJECTIVE:This study aimed to analyze systemic differences of innate and adaptive immune cells from healthy con...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000508699
更新日期:2020-01-01 00:00:00
abstract:BACKGROUND:High osmolal ionic radiocontrast media (RCM) cause vascular release of endothelin-1 (ET-1) and activate mast cells. Iomeprol, a nonionic RCM, has recently been reported not to activate cardiac mast cells. This coronary angiography study was performed to extend those findings using another nonionic RCM, iopro...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000070435
更新日期:2003-05-01 00:00:00
abstract:BACKGROUND:Numerous strategies have been proposed for the treatment of peanut allergies, but despite the steady advancement in our understanding of atopic immune responses and the increasing number of deaths each year from peanut anaphylaxis, there is still no safe, effective, specific therapy for the peanut-sensitive ...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000053672
更新日期:2001-01-01 00:00:00
abstract:BACKGROUND:2-Arachidonoylglycerol (2-AG), an endogenous ligand for the cannabinoid receptors (CB1 and CB2), has been shown to exhibit a variety of cannabimimetic activities in vitro and in vivo. Recently, we found that human eosinophilic leukemia EoL-1 cells and human peripheral blood eosinophils express the CB2 recept...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000092704
更新日期:2006-01-01 00:00:00
abstract:BACKGROUND:Assessing high-sensitivity C-reactive protein (hs-CRP) in relation to allergic endpoints can shed light on both the mechanisms of allergic disease development and early non-communicable disease prevention. However, only a few epidemiological studies so far have investigated the relationship in children and a...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章,多中心研究
doi:10.1159/000497320
更新日期:2019-01-01 00:00:00
abstract::Nonspecific hyperreactivity to a number of physical, chemical and pharmacological agents in a common characteristics of allergic subjects, which is strictly related to the inflammatory events consequent to an allergic reaction. Hyperosmolar stimulus has previously been shown to cause an inflammatory response inducing ...
journal_title:International archives of allergy and immunology
pub_type: 临床试验,杂志文章
doi:10.1159/000236713
更新日期:1994-05-01 00:00:00
abstract:BACKGROUND:In naive rodents, repeated exposure to aerosolized antigen induces suppression of the Th2 response to the antigen. We hypothesized that more prolonged exposure of established asthma model to antigen aerosols may downregulate asthmatic phenotype. METHODS:After establishing an ovalbumin (OVA)-induced asthma m...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000049503
更新日期:2001-10-01 00:00:00
abstract:BACKGROUND:Th17 is a subset of T-helper lymphocytes that produce proinflammatory cytokines, mainly IL-17. Serum IL-17 is increased in allergic patients and relates to clinical severity. Recently, it has been reported that CD161 is a highly upregulated gene in Th17 clones and all IL-17-producing cells are contained in C...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000330903
更新日期:2012-01-01 00:00:00
abstract::Aims of the Monaco Charter: (1) to present the current evidence on the efficacy and safety of allergen-specific immunotherapy (SIT) and to address the reasons for its underuse in clinical practice; (2) to develop strategies to increase the awareness about the benefits and the hazards of SIT in allergic patients, lay p...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000343883
更新日期:2013-01-01 00:00:00
abstract:BACKGROUND:Mast cell-released chemical mediators such as histamine, leukotriene (LT) C(4) and prostaglandin (PG) D(2) lead to the onset of allergic disorders. ATP provided from glycolysis is essential for histamine release and LTC(4) secretion from mast cells upon Fc epsilon RI cross-linking, indicating that glucose is...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000312122
更新日期:2010-01-01 00:00:00
abstract:BACKGROUND:Epinephrine autoinjector (EAI) is prescribed to prevent a fatal outcome in the case of a repeated anaphylactic reactions. We wanted to determine how adult patients who received their first prescription as part of an urgent treatment of an anaphylactic reaction or at their family physician were instructed on ...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000513421
更新日期:2021-01-22 00:00:00
abstract:BACKGROUND:Little information is available on eosinophil activation and the cytokine profile in virus-induced acute exacerbation of bronchial asthma; therefore, we examined the effects of treatments that included systemic corticosteroids on serum eosinophil cationic protein (ECP) and 17 cytokines/chemokines in rhinovir...
journal_title:International archives of allergy and immunology
pub_type: 临床试验,杂志文章
doi:10.1159/000327434
更新日期:2011-01-01 00:00:00
abstract:BACKGROUND:Basophils and mast cells are important initiator/effector cells capable of rapidly responding to IgE-mediated stimulation, but the precise mechanisms regulating their functions in vivo have not been fully identified. In this study, we assessed whether low levels of antigen can modulate activation of basophil...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000350339
更新日期:2013-01-01 00:00:00
abstract::To assess the role of infection in anti-DNA antibody production, the DNA-binding activity of sera from patients with Escherichia coli bacteremia was analyzed. Among 8 patients with bacteremia documented by blood culture, 5 demonstrated increased levels of antibodies to single-stranded DNA from E. coli as measured by e...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000236204
更新日期:1992-01-01 00:00:00
abstract::Eosinophils are pleiotropic multi-functional leukocytes involved in initiation and propagation of diverse inflammatory responses. Recent studies examining eosinophil biology have focused on delineating the molecular basis of FIP1L1/PDGRFalpha-fusion gene induced HES, the molecular steps involved in eosinophil recruitm...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章,评审
doi:10.1159/000101398
更新日期:2007-01-01 00:00:00
abstract:BACKGROUND:It has been shown that antigen presentation by resting B cells can induce tolerance to intravenously administered protein antigens, but the role of resting B cells in the induction of oral tolerance is unclear. METHODS:Mice continuously treated since birth with rabbit anti-mouse IgM serum for 5 weeks were d...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000024394
更新日期:2000-07-01 00:00:00
abstract:BACKGROUND:Atopic dermatitis (AD) is a chronic inflammatory skin disease characterized by the interplay between multiple genetic and environmental factors. The pathogenesis of AD remains incompletely understood. Treatment with topical steroids for chronic AD symptoms has severe side effects and so a new treatment is re...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000486958
更新日期:2018-01-01 00:00:00
abstract::Effects of mature T lymphocytes on thymic colonization by lymphohemopoietic cells were investigated in an in vitro experimental model, using a variety of experimental strategies. Lymphoid-depleted fetal thymus (FT) explants (C57BL/Ka, Thy1.1, H-2b) were incubated with bone marrow (BM) cells from syngeneic (C57BL/Ka; S...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000236884
更新日期:1995-01-01 00:00:00
abstract:BACKGROUND:The fractional concentration of exhaled nitric oxide (FeNO) is a surrogate biomarker for Th2-dependent bronchial inflammation. The present study investigated whether FeNO may characterize allergic rhinitis (AR) patients. METHODS:A total of 553 AR patients (497 males, mean age 28.8 years) were evaluated. Tho...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000456548
更新日期:2017-01-01 00:00:00
abstract:BACKGROUND:Exposure and sensitization to pet allergens are associated with allergic asthma in children. Conflicting data have emerged regarding the potential benefit of air cleaners with respect to a reduction of indoor pet allergens and bronchial hyperresponsiveness (BHR). METHODS:In a randomized controlled trial 36 ...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章,随机对照试验
doi:10.1159/000151502
更新日期:2009-01-01 00:00:00
abstract::Ovalbumin (OVA)-pullulan conjugates were made using four different conjugation methods and eight different molecular weights of pullulan ranging from 4,200 to 600,000. Pretreatment of mice by the administration of conjugates made by using cyanulic chloride or cyanogen bromide and pullulan of molecular weight 40,000 or...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000236536
更新日期:1993-01-01 00:00:00
abstract:BACKGROUND:In patients showing multiple reactivity to seasonal airborne allergens, a skin prick test (SPT) with Parietaria pollen scores frequently negative, suggesting that profilin from Parietaria might not share IgE-binding epitopes with profilin from botanically unrelated airborne allergens. This study investigated...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000076384
更新日期:2004-02-01 00:00:00
abstract:BACKGROUND:Allergic conjunctivitis is characterized by itchy, watery and swollen eyes which occur in response to exposure to seasonal or environmental allergens. The early phase reaction of allergic conjunctivitis is primarily mediated by mast cell degranulation while the late phase reaction is driven by Th2 cells and ...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000328769
更新日期:2012-01-01 00:00:00