Role of interactions between the origin recognition complex and SIR1 in transcriptional silencing.


:Transcriptional silencing of the HM mating-type loci in the yeast Saccharomyces cerevisiae is caused by the localized formation of an altered chromatin structure, analogous to heterochromatin in higher eukaryotes. Silencing depends on cis-acting sequences, termed silencers, as well as several trans-acting factors, including histones H4 and H3, proteins RAP1 and ABF1, and the four SIR proteins (SIR1-4). Each of the four HM silencers contains an autonomously replicating sequence (ARS) to which the origin replication complex (ORC) binds. This six-protein complex is required for initiation of DNA replication, as well as for silencing. Efficient establishment of the silenced state requires both passage through the S phase of the cell cycle and SIR1 protein. Previous experiments suggested that SIR1 might be localized to the silencers by binding to ORC and/or RAP1. Here we report that SIR1 can bind directly to ORC1, the largest of the ORC subunits, and that targeting of SIR1 to ORC1 at a silencer is sufficient to establish a silenced state.






Triolo T,Sternglanz R




Has Abstract


1996-05-16 00:00:00














  • Two disparate ligand-binding sites in the human P2Y1 receptor.

    abstract::In response to adenosine 5'-diphosphate, the P2Y1 receptor (P2Y1R) facilitates platelet aggregation, and thus serves as an important antithrombotic drug target. Here we report the crystal structures of the human P2Y1R in complex with a nucleotide antagonist MRS2500 at 2.7 Å resolution, and with a non-nucleotide antago...


    pub_type: 杂志文章


    authors: Zhang D,Gao ZG,Zhang K,Kiselev E,Crane S,Wang J,Paoletta S,Yi C,Ma L,Zhang W,Han GW,Liu H,Cherezov V,Katritch V,Jiang H,Stevens RC,Jacobson KA,Zhao Q,Wu B

    更新日期:2015-04-16 00:00:00

  • Naloxone-reversible effect of opioids on pinocytosis in Amoeba proteus.

    abstract::A characteristic feature of induced pinocytosis in Amoeba proteus is the formation of broad channels by invagination of the cell membrane. This process, which requires Ca2+, occurs in response to depolarising cations. High Ca2+ levels reduce pinocytosis induced by cations such as Na+ and Tris+, whereas pinocytosis ind...


    pub_type: 杂志文章


    authors: Josefsson JO,Johansson P

    更新日期:1979-11-01 00:00:00

  • Lagging-strand replication shapes the mutational landscape of the genome.

    abstract::The origin of mutations is central to understanding evolution and of key relevance to health. Variation occurs non-randomly across the genome, and mechanisms for this remain to be defined. Here we report that the 5' ends of Okazaki fragments have significantly increased levels of nucleotide substitution, indicating a ...


    pub_type: 杂志文章


    authors: Reijns MAM,Kemp H,Ding J,de Procé SM,Jackson AP,Taylor MS

    更新日期:2015-02-26 00:00:00

  • Entanglement of two quantum memories via fibres over dozens of kilometres.

    abstract::A quantum internet that connects remote quantum processors1,2 should enable a number of revolutionary applications such as distributed quantum computing. Its realization will rely on entanglement of remote quantum memories over long distances. Despite enormous progress3-12, at present the maximal physical separation a...


    pub_type: 杂志文章


    authors: Yu Y,Ma F,Luo XY,Jing B,Sun PF,Fang RZ,Yang CW,Liu H,Zheng MY,Xie XP,Zhang WJ,You LX,Wang Z,Chen TY,Zhang Q,Bao XH,Pan JW

    更新日期:2020-02-01 00:00:00

  • Electrical effects of spin density wave quantization and magnetic domain walls in chromium.

    abstract::The role of magnetic domains (and the walls between domains) in determining the electrical properties of ferromagnetic materials has been investigated in great detail for many years, not least because control over domains offers a means of manipulating electron spin to control charge transport in 'spintronic' devices....


    pub_type: 杂志文章


    authors: Kummamuru RK,Soh YA

    更新日期:2008-04-17 00:00:00

  • Immunogenic BNT162b vaccines protect rhesus macaques from SARS-CoV-2.

    abstract::A safe and effective vaccine against COVID-19 is urgently needed in quantities sufficient to immunise large populations. We report the preclinical development of two BNT162b vaccine candidates, which contain lipid-nanoparticle (LNP) formulated nucleoside-modified mRNA encoding SARS-CoV-2 spike glycoprotein-derived imm...


    pub_type: 杂志文章


    authors: Vogel AB,Kanevsky I,Che Y,Swanson KA,Muik A,Vormehr M,Kranz LM,Walzer KC,Hein S,Güler A,Loschko J,Maddur MS,Ota-Setlik A,Tompkins K,Cole J,Lui BG,Ziegenhals T,Plaschke A,Eisel D,Dany SC,Fesser S,Erbar S,Bates

    更新日期:2021-02-01 00:00:00

  • A semi-synthetic organism that stores and retrieves increased genetic information.

    abstract::Since at least the last common ancestor of all life on Earth, genetic information has been stored in a four-letter alphabet that is propagated and retrieved by the formation of two base pairs. The central goal of synthetic biology is to create new life forms and functions, and the most general route to this goal is th...


    pub_type: 杂志文章


    authors: Zhang Y,Ptacin JL,Fischer EC,Aerni HR,Caffaro CE,San Jose K,Feldman AW,Turner CR,Romesberg FE

    更新日期:2017-11-29 00:00:00

  • In vitro effects of high molecular weight forms of ACTH on the fetal sheep adrenal.

    abstract::The direct involvement of the pituitary-adrenal axis in birth has been well established, at least in sheep, and its removal prolongs pregnancy. As part of the process the fetal sheep adrenal grows rapidly during the 10-15 d prepartum and is associated with a large rise in the plasma corticosteroid concentration. This ...


    pub_type: 杂志文章


    authors: Roebuck MM,Jones CT,Holland D,Silman R

    更新日期:1980-04-17 00:00:00

  • Cyclin D-CDK4 kinase destabilizes PD-L1 via cullin 3-SPOP to control cancer immune surveillance.

    abstract::Treatments that target immune checkpoints, such as the one mediated by programmed cell death protein 1 (PD-1) and its ligand PD-L1, have been approved for treating human cancers with durable clinical benefit. However, many patients with cancer fail to respond to compounds that target the PD-1 and PD-L1 interaction, an...


    pub_type: 杂志文章


    authors: Zhang J,Bu X,Wang H,Zhu Y,Geng Y,Nihira NT,Tan Y,Ci Y,Wu F,Dai X,Guo J,Huang YH,Fan C,Ren S,Sun Y,Freeman GJ,Sicinski P,Wei W

    更新日期:2018-01-04 00:00:00

  • Balanced responsiveness to chemoattractants from adjacent zones determines B-cell position.

    abstract::B lymphocytes re-circulate between B-cell-rich compartments (follicles or B zones) in secondary lymphoid organs, surveying for antigen. After antigen binding, B cells move to the boundary of B and T zones to interact with T-helper cells. Despite the importance of B--T-cell interactions for the induction of antibody re...


    pub_type: 杂志文章


    authors: Reif K,Ekland EH,Ohl L,Nakano H,Lipp M,Förster R,Cyster JG

    更新日期:2002-03-07 00:00:00

  • Resolving the long-standing enigmas of a giant ornithomimosaur Deinocheirus mirificus.

    abstract::The holotype of Deinocheirus mirificus was collected by the 1965 Polish-Mongolian Palaeontological Expedition at Altan Uul III in the southern Gobi of Mongolia. Because the holotype consists mostly of giant forelimbs (2.4 m in length) with scapulocoracoids, for almost 50 years Deinocheirus has remained one of the most...


    pub_type: 杂志文章


    authors: Lee YN,Barsbold R,Currie PJ,Kobayashi Y,Lee HJ,Godefroit P,Escuillié F,Chinzorig T

    更新日期:2014-11-13 00:00:00

  • Sleep behaviour: sleep in continuously active dolphins.

    abstract::Sleep has been assumed to be necessary for development and to be a vital function in mammals and other animals. However, Lyamin et al. claim that in bottlenose dolphins (Tursiops truncatus) and killer whales (Orcinus orca), neonates and their mothers show almost no sleep behaviour for the first month after birth; this...


    pub_type: 评论,杂志文章


    authors: Sekiguchi Y,Arai K,Kohshima S

    更新日期:2006-06-22 00:00:00

  • A dispersive morph in the naked mole-rat.

    abstract::Close inbreeding is known for a variety of small mammal species for which a high probability of mortality during dispersal makes helping and delayed maturation a relatively secure fitness option. Prolonged inbreeding, however, is usually associated with lowered fitness, and it has been shown that most highly inbred sm...


    pub_type: 杂志文章


    authors: O'Riain MJ,Jarvis JU,Faulkes CG

    更新日期:1996-04-18 00:00:00

  • An integrated model of kimberlite ascent and eruption.

    abstract::Diatremes are carrot-shaped bodies forming the upper parts of very deep magmatic intrusions of kimberlite rock. These unusual, enigmatic and complex features are famous as the source of diamonds. Here we present a new model of kimberlite ascent and eruption, emphasizing the extremely unsteady nature of this process to...


    pub_type: 杂志文章


    authors: Wilson L,Head Iii JW 3rd

    更新日期:2007-05-03 00:00:00

  • RNA interference in adult mice.

    abstract::RNA interference is an evolutionarily conserved surveillance mechanism that responds to double-stranded RNA by sequence-specific silencing of homologous genes. Here we show that transgene expression can be suppressed in adult mice by synthetic small interfering RNAs and by small-hairpin RNAs transcribed in vivo from D...


    pub_type: 杂志文章


    authors: McCaffrey AP,Meuse L,Pham TT,Conklin DS,Hannon GJ,Kay MA

    更新日期:2002-07-04 00:00:00

  • A motif in the alphabeta T-cell receptor controls positive selection by modulating ERK activity.

    abstract::Positive selection allows thymocytes that recognize an individual's own major histocompatibility complex (self-MHC) molecules to survive and differentiate, whereas negative selection removes overtly self-reactive thymocytes. Although both forms of thymic selection are mediated by the alphabeta T-cell receptor (TCR) an...


    pub_type: 杂志文章


    authors: Werlen G,Hausmann B,Palmer E

    更新日期:2000-07-27 00:00:00

  • Martian dust storm impact on atmospheric H2O and D/H observed by ExoMars Trace Gas Orbiter.

    abstract::Global dust storms on Mars are rare1,2 but can affect the Martian atmosphere for several months. They can cause changes in atmospheric dynamics and inflation of the atmosphere3, primarily owing to solar heating of the dust3. In turn, changes in atmospheric dynamics can affect the distribution of atmospheric water vapo...


    pub_type: 杂志文章


    authors: Vandaele AC,Korablev O,Daerden F,Aoki S,Thomas IR,Altieri F,López-Valverde M,Villanueva G,Liuzzi G,Smith MD,Erwin JT,Trompet L,Fedorova AA,Montmessin F,Trokhimovskiy A,Belyaev DA,Ignatiev NI,Luginin M,Olsen KS,Baggi

    更新日期:2019-04-01 00:00:00

  • Ensuring meiotic DNA break formation in the mouse pseudoautosomal region.

    abstract::Sex chromosomes in males of most eutherian mammals share only a small homologous segment, the pseudoautosomal region (PAR), in which the formation of double-strand breaks (DSBs), pairing and crossing over must occur for correct meiotic segregation1,2. How cells ensure that recombination occurs in the PAR is unknown. H...


    pub_type: 杂志文章


    authors: Acquaviva L,Boekhout M,Karasu ME,Brick K,Pratto F,Li T,van Overbeek M,Kauppi L,Camerini-Otero RD,Jasin M,Keeney S

    更新日期:2020-06-01 00:00:00

  • Potent antitumour activity of a new class of tumour-specific killer cells.

    abstract::Two approaches to the antibody-directed targeting of toxic or cytolytic activity and augmentation of cellular immune responses have been explored for tumour immunotherapy, but so far success has been limited. Obstacles facing immunotherapy are the limited accessibility of antibodies or antibody conjugates to solid tum...


    pub_type: 杂志文章


    authors: Chen SY,Yang AG,Chen JD,Kute T,King CR,Collier J,Cong Y,Yao C,Huang XF

    更新日期:1997-01-02 00:00:00

  • Worries about infectious cancer.

    abstract::The recent discovery that the human T-cell lymphoma leukemia virus (HTLV) may be the agent of transmission of T-cell lymphoid malignancies raises ethical questions for blood transfusion services. The effort to avoid use of contaminated blood is dependent to a large extent on the willingness and ability of donors to g...


    pub_type: 新闻



    更新日期:1983-04-14 00:00:00

  • Mapping GFP structure evolution during proton transfer with femtosecond Raman spectroscopy.

    abstract::Tracing the transient atomic motions that lie at the heart of chemical reactions requires high-resolution multidimensional structural information on the timescale of molecular vibrations, which commonly range from 10 fs to 1 ps. For simple chemical systems, it has been possible to map out in considerable detail the re...


    pub_type: 杂志文章


    authors: Fang C,Frontiera RR,Tran R,Mathies RA

    更新日期:2009-11-12 00:00:00

  • PTEN counteracts FBXL2 to promote IP3R3- and Ca2+-mediated apoptosis limiting tumour growth.

    abstract::In response to environmental cues that promote IP3 (inositol 1,4,5-trisphosphate) generation, IP3 receptors (IP3Rs) located on the endoplasmic reticulum allow the 'quasisynaptical' feeding of calcium to the mitochondria to promote oxidative phosphorylation. However, persistent Ca2+ release results in mitochondrial Ca2...


    pub_type: 杂志文章


    authors: Kuchay S,Giorgi C,Simoneschi D,Pagan J,Missiroli S,Saraf A,Florens L,Washburn MP,Collazo-Lorduy A,Castillo-Martin M,Cordon-Cardo C,Sebti SM,Pinton P,Pagano M

    更新日期:2017-06-22 00:00:00

  • Messenger RNA for the coat protein of tobacco mosaic virus.

    abstract::TMV RNA is not an efficient template for translation of the viral coat protein, in spite of containing nucleotide sequences coding for the protein. Efficient translation requires the prior synthesis within infected cells of a smaller RNA carrying only a portion of the information encoded in the whole genome. ...


    pub_type: 杂志文章


    authors: Hunter TR,Hunt T,Knowland J,Zimmern D

    更新日期:1976-04-29 00:00:00

  • Pre-bending of a promoter sequence enhances affinity for the TATA-binding factor.

    abstract::TATA-binding protein (TBP) binds the minor groove of the TATA element with the DNA bent 80 degrees towards the major groove. A constrained minicircle strategy has been used to test the effect of DNA topology on the affinity of TBP for the TATA element. We report here that TBP bound to DNA which was slightly pre-bent t...


    pub_type: 杂志文章


    authors: Parvin JD,McCormick RJ,Sharp PA,Fisher DE

    更新日期:1995-02-23 00:00:00

  • Ab initio alpha-alpha scattering.

    abstract::Processes such as the scattering of alpha particles ((4)He), the triple-alpha reaction, and alpha capture play a major role in stellar nucleosynthesis. In particular, alpha capture on carbon determines the ratio of carbon to oxygen during helium burning, and affects subsequent carbon, neon, oxygen, and silicon burning...


    pub_type: 杂志文章


    authors: Elhatisari S,Lee D,Rupak G,Epelbaum E,Krebs H,Lähde TA,Luu T,Meißner UG

    更新日期:2015-12-03 00:00:00

  • A novel eye in 'eyeless' shrimp from hydrothermal vents of the Mid-Atlantic Ridge.

    abstract::Rimicaris exoculata is a shrimp that swarms over high-temperature (350 degrees C) sulphide chimneys at Mid-Atlantic Ridge hydrothermal fields (3,600 m). This shrimp lacks an externally differentiated eye, having instead a pair of large organs within the cephalothorax immediately beneath the dorsal surface of the trans...


    pub_type: 杂志文章


    authors: Van Dover CL,Szuts EZ,Chamberlain SC,Cann JR

    更新日期:1989-02-02 00:00:00

  • Is multiple sulphatase deficiency due to defective regulation of sulphohydrolase expression?

    abstract::Multiple sulphatase deficiency disease is an unusual autosomal recessive disorder characterised biochemically by a deficiency of several sulphohydrolase activities. The laboratory diagnosis of this combined neurological connective tissue disorder is made on the basis of decreased activities of the lysosomal enzymes, a...


    pub_type: 杂志文章


    authors: Fiddler MB,Vine D,Shapira E,Nadler HL

    更新日期:1979-11-01 00:00:00

  • Proliferation of mature oligodendrocytes after trauma to the central nervous system.

    abstract::It has long been thought that mature oligodendrocytes in the adult mammalian central nervous system (CNS) are post-mitotic and are unable to proliferate in response to injury. The implications of this have been profound, because it has been suggested that this failure of oligodendrocytes to undergo mitosis is perhaps ...


    pub_type: 杂志文章


    authors: Ludwin SK

    更新日期:1984-03-15 00:00:00

  • Enzymatic replication of E. coli chromosomal origin is bidirectional.

    abstract::A soluble enzyme system has been discovered which specifically recognizes and replicates plasmids containing the Escherichia coli chromosomal origin, oriC. Electron microscopy has shown that plasmid replication begins at or near oriC from which it progresses bidirectionally to completion. Control of initiation of a cy...


    pub_type: 杂志文章


    authors: Kaguni JM,Fuller RS,Kornberg A

    更新日期:1982-04-15 00:00:00

  • Synaptic, transcriptional and chromatin genes disrupted in autism.

    abstract::The genetic architecture of autism spectrum disorder involves the interplay of common and rare variants and their impact on hundreds of genes. Using exome sequencing, here we show that analysis of rare coding variation in 3,871 autism cases and 9,937 ancestry-matched or parental controls implicates 22 autosomal genes ...


    pub_type: 杂志文章


    authors: De Rubeis S,He X,Goldberg AP,Poultney CS,Samocha K,Cicek AE,Kou Y,Liu L,Fromer M,Walker S,Singh T,Klei L,Kosmicki J,Shih-Chen F,Aleksic B,Biscaldi M,Bolton PF,Brownfeld JM,Cai J,Campbell NG,Carracedo A,Chahrour

    更新日期:2014-11-13 00:00:00