Role of interactions between the origin recognition complex and SIR1 in transcriptional silencing.


:Transcriptional silencing of the HM mating-type loci in the yeast Saccharomyces cerevisiae is caused by the localized formation of an altered chromatin structure, analogous to heterochromatin in higher eukaryotes. Silencing depends on cis-acting sequences, termed silencers, as well as several trans-acting factors, including histones H4 and H3, proteins RAP1 and ABF1, and the four SIR proteins (SIR1-4). Each of the four HM silencers contains an autonomously replicating sequence (ARS) to which the origin replication complex (ORC) binds. This six-protein complex is required for initiation of DNA replication, as well as for silencing. Efficient establishment of the silenced state requires both passage through the S phase of the cell cycle and SIR1 protein. Previous experiments suggested that SIR1 might be localized to the silencers by binding to ORC and/or RAP1. Here we report that SIR1 can bind directly to ORC1, the largest of the ORC subunits, and that targeting of SIR1 to ORC1 at a silencer is sufficient to establish a silenced state.






Triolo T,Sternglanz R




Has Abstract


1996-05-16 00:00:00














  • Ploughing the deep sea floor.

    abstract::Bottom trawling is a non-selective commercial fishing technique whereby heavy nets and gear are pulled along the sea floor. The direct impact of this technique on fish populations and benthic communities has received much attention, but trawling can also modify the physical properties of seafloor sediments, water–sedi...


    pub_type: 杂志文章


    authors: Puig P,Canals M,Company JB,Martín J,Amblas D,Lastras G,Palanques A

    更新日期:2012-09-13 00:00:00

  • Cyclosporin A promotes spontaneous outgrowth in vitro of Epstein-Barr virus-induced B-cell lines.

    abstract::Cyclosporin A (CSA) is a fungal metabolite which exerts profound effects on the immune system and has potential as a selective immuno-suppressive agent. Clinical trials with human renal allograft recipients have confirmed this potential but there have been disturbing reports of lymphoma in a significant number of pati...


    pub_type: 杂志文章


    authors: Bird AG,McLachlan SM,Britton S

    更新日期:1981-01-22 00:00:00

  • Inhibition of RNA cleavage but not polyadenylation by a point mutation in mRNA 3' consensus sequence AAUAAA.

    abstract::A single U leads to G transversion in the 3' consensus sequence AAUAAA of the adenovirus early region 1A gene was constructed and the effect of this mutation on processing of the 3' end of the nuclear early region 1A RNAs was analysed. The results demonstrate that the intact AAUAAA is not required for RNA polyadenylat...


    pub_type: 杂志文章


    authors: Montell C,Fisher EF,Caruthers MH,Berk AJ

    更新日期:1983-10-13 00:00:00

  • Structural basis for retroviral integration into nucleosomes.

    abstract::Retroviral integration is catalysed by a tetramer of integrase (IN) assembled on viral DNA ends in a stable complex, known as the intasome. How the intasome interfaces with chromosomal DNA, which exists in the form of nucleosomal arrays, is currently unknown. Here we show that the prototype foamy virus (PFV) intasome ...


    pub_type: 杂志文章


    authors: Maskell DP,Renault L,Serrao E,Lesbats P,Matadeen R,Hare S,Lindemann D,Engelman AN,Costa A,Cherepanov P

    更新日期:2015-07-16 00:00:00

  • Potassium channel receptor site for the inactivation gate and quaternary amine inhibitors.

    abstract::Many voltage-dependent K+ channels open when the membrane is depolarized and then rapidly close by a process called inactivation. Neurons use inactivating K+ channels to modulate their firing frequency. In Shaker-type K+ channels, the inactivation gate, which is responsible for the closing of the channel, is formed by...


    pub_type: 杂志文章


    authors: Zhou M,Morais-Cabral JH,Mann S,MacKinnon R

    更新日期:2001-06-07 00:00:00

  • Imidazole inhibits a temperature-dependent component of mammalial skeletal muscle action potential.

    abstract::Temperature-dependent transition of conductance in ionic channels has been described in several membrane systems. We report here an abrupt change in the maximal rate of rise of the action potentials of the rat extensor digitorum longus (EDL) at 32 degrees C, indicating alteration in the functional characteristics of t...


    pub_type: 杂志文章


    authors: Cheung DW,Daniel EE

    更新日期:1980-01-31 00:00:00

  • Isolation of a spermatozoid-releasing and -attracting substance from female gametophytes of Laminaria digitata.

    abstract::SEVERAL dioecious marine brown algae have been valuable in studying chemical communication during sexual reproduction. For example, female gametes of Ectocarpus siliculosus, Cutleria multifida and Fucus serratus secrete olefinic hydrocarbons into the seawater to attract male gametes(1-3). A more complex system is foun...


    pub_type: 杂志文章


    authors: Müller DG,Gassmann G,Lüning K

    更新日期:1979-05-31 00:00:00

  • Universal structure of dark matter haloes over a mass range of 20 orders of magnitude.

    abstract::Cosmological models in which dark matter consists of cold elementary particles predict that the dark halo population should extend to masses many orders of magnitude below those at which galaxies can form1-3. Here we report a cosmological simulation of the formation of present-day haloes over the full range of observe...


    pub_type: 杂志文章


    authors: Wang J,Bose S,Frenk CS,Gao L,Jenkins A,Springel V,White SDM

    更新日期:2020-09-01 00:00:00

  • Quantum critical behaviour in a high-T(c) superconductor.

    abstract::Quantum criticality is associated with a system composed of a nearly infinite number of interacting quantum degrees of freedom at zero temperature, and it implies that the system looks on average the same regardless of the time- and length scale on which it is observed. Electrons on the atomic scale do not exhibit suc...


    pub_type: 杂志文章


    authors: van der Marel D,Molegraaf HJ,Zaanen J,Nussinov Z,Carbone F,Damascelli A,Eisaki H,Greven M,Kes PH,Li M

    更新日期:2003-09-18 00:00:00

  • Drosophila Stardust is a partner of Crumbs in the control of epithelial cell polarity.

    abstract::The polarized architecture of epithelial cells depends on the highly stereotypic distribution of cellular junctions and other membrane-associated protein complexes. In epithelial cells of the Drosophila embryo, three distinct domains subdivide the lateral plasma membrane. The most apical one comprises the subapical co...


    pub_type: 杂志文章


    authors: Bachmann A,Schneider M,Theilenberg E,Grawe F,Knust E

    更新日期:2001-12-06 00:00:00

  • A giant stream of metal-rich stars in the halo of the galaxy M31.

    abstract::Recent observations have revealed streams of gas and stars in the halo of the Milky Way that are the debris from interactions between our Galaxy and some of its dwarf companion galaxies; the Sagittarius dwarf galaxy and the Magellanic clouds. Analysis of the material has shown that much of the halo is made up of canni...


    pub_type: 杂志文章


    authors: Ibata R,Irwin M,Lewis G,Ferguson AM,Tanvir N

    更新日期:2001-07-05 00:00:00

  • Splicing factor Sub2p is required for nuclear mRNA export through its interaction with Yra1p.

    abstract::The yeast nuclear protein Yra1p is an essential export factor for mRNA. Yra1p interacts directly with the mRNA transport factor Mex67p/Mtr2p, which is associated with the nuclear pore. Here, we report a genetic interaction between YRA1 and SUB2, the gene for a DEAD box helicase involved in splicing. Mutation of SUB2 a...


    pub_type: 杂志文章


    authors: Strässer K,Hurt E

    更新日期:2001-10-11 00:00:00

  • Imaging of Titan from the Cassini spacecraft.

    abstract::Titan, the largest moon of Saturn, is the only satellite in the Solar System with a substantial atmosphere. The atmosphere is poorly understood and obscures the surface, leading to intense speculation about Titan's nature. Here we present observations of Titan from the imaging science experiment onboard the Cassini sp...


    pub_type: 杂志文章


    authors: Porco CC,Baker E,Barbara J,Beurle K,Brahic A,Burns JA,Charnoz S,Cooper N,Dawson DD,Del Genio AD,Denk T,Dones L,Dyudina U,Evans MW,Fussner S,Giese B,Grazier K,Helfenstein P,Ingersoll AP,Jacobson RA,Johnson TV,McE

    更新日期:2005-03-10 00:00:00

  • Brain damage: neglect disrupts the mental number line.

    abstract::A popular metaphor for the representation of numbers in the brain is the 'mental number line', in which numbers are represented in a continuous, quantity-based analogical format. Here we show that patients with hemispatial neglect misplace the midpoint of a numerical interval when asked to bisect it (for example, stat...


    pub_type: 杂志文章


    authors: Zorzi M,Priftis K,Umiltà C

    更新日期:2002-05-09 00:00:00

  • Glycosphingolipid synthesis requires FAPP2 transfer of glucosylceramide.

    abstract::The molecular machinery responsible for the generation of transport carriers moving from the Golgi complex to the plasma membrane relies on a tight interplay between proteins and lipids. Among the lipid-binding proteins of this machinery, we previously identified the four-phosphate adaptor protein FAPP2, the pleckstri...


    pub_type: 杂志文章


    authors: D'Angelo G,Polishchuk E,Di Tullio G,Santoro M,Di Campli A,Godi A,West G,Bielawski J,Chuang CC,van der Spoel AC,Platt FM,Hannun YA,Polishchuk R,Mattjus P,De Matteis MA

    更新日期:2007-09-06 00:00:00

  • A pathogenic picornavirus acquires an envelope by hijacking cellular membranes.

    abstract::Animal viruses are broadly categorized structurally by the presence or absence of an envelope composed of a lipid-bilayer membrane, attributes that profoundly affect stability, transmission and immune recognition. Among those lacking an envelope, the Picornaviridae are a large and diverse family of positive-strand RNA...


    pub_type: 杂志文章


    authors: Feng Z,Hensley L,McKnight KL,Hu F,Madden V,Ping L,Jeong SH,Walker C,Lanford RE,Lemon SM

    更新日期:2013-04-18 00:00:00

  • Identification of a mast-cell-specific receptor crucial for pseudo-allergic drug reactions.

    abstract::Mast cells are primary effectors in allergic reactions, and may have important roles in disease by secreting histamine and various inflammatory and immunomodulatory substances. Although they are classically activated by immunoglobulin (Ig)E antibodies, a unique property of mast cells is their antibody-independent resp...


    pub_type: 杂志文章


    authors: McNeil BD,Pundir P,Meeker S,Han L,Undem BJ,Kulka M,Dong X

    更新日期:2015-03-12 00:00:00

  • Systematic variation of the stellar initial mass function in early-type galaxies.

    abstract::Much of our knowledge of galaxies comes from analysing the radiation emitted by their stars, which depends on the present number of each type of star in the galaxy. The present number depends on the stellar initial mass function (IMF), which describes the distribution of stellar masses when the population formed, and ...


    pub_type: 杂志文章


    authors: Cappellari M,McDermid RM,Alatalo K,Blitz L,Bois M,Bournaud F,Bureau M,Crocker AF,Davies RL,Davis TA,de Zeeuw PT,Duc PA,Emsellem E,Khochfar S,Krajnović D,Kuntschner H,Lablanche PY,Morganti R,Naab T,Oosterloo T,Sarz

    更新日期:2012-04-25 00:00:00

  • The role of nucleotide sequences in splice site selection in eukaryotic pre-messenger RNA.

    abstract::Alternative splicing of eukaryotic messenger RNA precursors is now known to be of widespread importance in generating multiple transcripts from a single gene. This phenomenon has emphasized the problem of the way in which splice sites are selected; recent studies have discussed the role of secondary structure or affin...


    pub_type: 杂志文章


    authors: Eperon LP,Estibeiro JP,Eperon IC

    更新日期:1986-11-20 00:00:00

  • Quantum suppression of superconductivity in ultrathin nanowires

    abstract::It is of fundamental importance to establish whether there is a limit to how thin a superconducting wire can be, while retaining its superconducting character--and if there is a limit, to determine what sets it. This issue may also be of practical importance in defining the limit to miniaturization of superconducting ...


    pub_type: 杂志文章


    authors: Bezryadin A,Lau CN,Tinkham M

    更新日期:2000-04-27 00:00:00

  • Pseudoautosomal DNA sequences in the pairing region of the human sex chromosomes.

    abstract::A DNA probe from a human Y chromosome-derived cosmid detects a single-copy genomic DNA fragment which can appear in different allelic forms shared by both sex chromosomes. Variants at this DNA locus show an autosomal pattern of inheritance, undergo recombination with sexual phenotype and can therefore be described as ...


    pub_type: 杂志文章


    authors: Simmler MC,Rouyer F,Vergnaud G,Nyström-Lahti M,Ngo KY,de la Chapelle A,Weissenbach J

    更新日期:1985-10-24 00:00:00

  • In vitro effects of high molecular weight forms of ACTH on the fetal sheep adrenal.

    abstract::The direct involvement of the pituitary-adrenal axis in birth has been well established, at least in sheep, and its removal prolongs pregnancy. As part of the process the fetal sheep adrenal grows rapidly during the 10-15 d prepartum and is associated with a large rise in the plasma corticosteroid concentration. This ...


    pub_type: 杂志文章


    authors: Roebuck MM,Jones CT,Holland D,Silman R

    更新日期:1980-04-17 00:00:00

  • International trade drives biodiversity threats in developing nations.

    abstract::Human activities are causing Earth's sixth major extinction event-an accelerating decline of the world's stocks of biological diversity at rates 100 to 1,000 times pre-human levels. Historically, low-impact intrusion into species habitats arose from local demands for food, fuel and living space. However, in today's in...


    pub_type: 杂志文章


    authors: Lenzen M,Moran D,Kanemoto K,Foran B,Lobefaro L,Geschke A

    更新日期:2012-06-06 00:00:00

  • Possible interaction between baryons and dark-matter particles revealed by the first stars.

    abstract::The cosmic radio-frequency spectrum is expected to show a strong absorption signal corresponding to the 21-centimetre-wavelength transition of atomic hydrogen around redshift 20, which arises from Lyman-α radiation from some of the earliest stars. By observing this 21-centimetre signal-either its sky-averaged spectrum...


    pub_type: 杂志文章


    authors: Barkana R

    更新日期:2018-02-28 00:00:00

  • Sequence and expression of a frog brain complementary DNA encoding a kainate-binding protein.

    abstract::Excitatory amino acids (EAAs) are important neurotransmitters in the vertebrate central nervous system. Electrophysiological and ligand-binding studies indicate that at least three different receptor subtypes for EAAs exist--N-methyl-D-aspartate, kainate and quisqualate receptor subtypes--on the basis of the preferred...


    pub_type: 杂志文章


    authors: Wada K,Dechesne CJ,Shimasaki S,King RG,Kusano K,Buonanno A,Hampson DR,Banner C,Wenthold RJ,Nakatani Y

    更新日期:1989-12-07 00:00:00

  • Constraints on the composition of the Earth's core from ab initio calculations

    abstract::Knowledge of the composition of the Earth's core is important for understanding its melting point and therefore the temperature at the inner-core boundary and the temperature profile of the core and mantle. In addition, the partitioning of light elements between solid and liquid, as the outer core freezes at the inner...


    pub_type: 杂志文章


    authors: Alfe D,Gillan MJ,Price GD

    更新日期:2000-05-11 00:00:00

  • Identification of the transforming EML4-ALK fusion gene in non-small-cell lung cancer.

    abstract::Improvement in the clinical outcome of lung cancer is likely to be achieved by identification of the molecular events that underlie its pathogenesis. Here we show that a small inversion within chromosome 2p results in the formation of a fusion gene comprising portions of the echinoderm microtubule-associated protein-l...


    pub_type: 杂志文章


    authors: Soda M,Choi YL,Enomoto M,Takada S,Yamashita Y,Ishikawa S,Fujiwara S,Watanabe H,Kurashina K,Hatanaka H,Bando M,Ohno S,Ishikawa Y,Aburatani H,Niki T,Sohara Y,Sugiyama Y,Mano H

    更新日期:2007-08-02 00:00:00

  • A hypothalamic novelty signal modulates hippocampal memory.

    abstract::The ability to recognize information that is incongruous with previous experience is critical for survival. Novelty signals have therefore evolved in the mammalian brain to enhance attention, perception and memory1,2. Although the importance of regions such as the ventral tegmental area3,4 and locus coeruleus5 in broa...


    pub_type: 杂志文章


    authors: Chen S,He L,Huang AJY,Boehringer R,Robert V,Wintzer ME,Polygalov D,Weitemier AZ,Tao Y,Gu M,Middleton SJ,Namiki K,Hama H,Therreau L,Chevaleyre V,Hioki H,Miyawaki A,Piskorowski RA,McHugh TJ

    更新日期:2020-10-01 00:00:00

  • Neurons that regulate mouse torpor.

    abstract::The advent of endothermy, which is achieved through the continuous homeostatic regulation of body temperature and metabolism1,2, is a defining feature of mammalian and avian evolution. However, when challenged by food deprivation or harsh environmental conditions, many mammalian species initiate adaptive energy-conser...


    pub_type: 杂志文章


    authors: Hrvatin S,Sun S,Wilcox OF,Yao H,Lavin-Peter AJ,Cicconet M,Assad EG,Palmer ME,Aronson S,Banks AS,Griffith EC,Greenberg ME

    更新日期:2020-07-01 00:00:00

  • eIF4F is a nexus of resistance to anti-BRAF and anti-MEK cancer therapies.

    abstract::In BRAF(V600)-mutant tumours, most mechanisms of resistance to drugs that target the BRAF and/or MEK kinases rely on reactivation of the RAS-RAF-MEK-ERK mitogen-activated protein kinase (MAPK) signal transduction pathway, on activation of the alternative, PI(3)K-AKT-mTOR, pathway (which is ERK independent) or on modul...


    pub_type: 杂志文章


    authors: Boussemart L,Malka-Mahieu H,Girault I,Allard D,Hemmingsson O,Tomasic G,Thomas M,Basmadjian C,Ribeiro N,Thuaud F,Mateus C,Routier E,Kamsu-Kom N,Agoussi S,Eggermont AM,Désaubry L,Robert C,Vagner S

    更新日期:2014-09-04 00:00:00