Potentiation of the anti-tumor effect of actinomycin D by tumor necrosis factor alpha in mice: correlation between in vitro and in vivo results.


:The anti-tumor effects of actinomycin D (Act D) and recombinant human tumor necrosis factor (TNF)-alpha have been studied on 4 established murine tumor cell lines: MmB16 melanoma, Lewis lung (LL/2) carcinoma, L1 sarcoma and L1210 leukemia. During short-term incubation (24 hr) Act D produced dose-dependent cytostatic/cytotoxic effects against MmB16, LL/2 and L1 tumor cells but did not reduce the viability of these cells even at high concentration (10 micrograms/ml), below a threshold of 30-60%. However, L1210 leukemic cells were highly susceptible to Act D, and no viable cells were detected in cultures incubated with 1 microgram/ml of Act D. TNF-alpha alone, when used under the same culture conditions, had only a negligible effect on all cell lines tested. However, the combination of this cytokine with Act D produced synergistic cytotoxic effects against MmB16, LL/2 and L1 cells but not against L1210 leukemia cells. In an in vivo model of regional therapy in which tumor-bearing mice were treated with Act D and TNF-alpha, a correlation with in vitro results was observed. In mice bearing MmB16 melanoma, LL/2 carcinoma and L1 sarcoma, the most potent anti-tumor effects were observed in mice treated with Act D and TNF-alpha together. This treatment led to a delay of tumor growth and induced complete tumor regression in some cases. On the contrary, TNF-alpha did not enhance the effect of Act D in mice injected with L1210 leukemia cells. Our results show that TNF-alpha can potentiate the anti-tumor effects of Act D against tumors weakly susceptible to Act D and may be a useful adjuvant to chemotherapy in the local treatment of neoplasia.


Int J Cancer


Lasek W,Giermasz A,Kuc K,Wańkowicz A,Feleszko W,Gołab J,Zagozdzon R,Stokłosa T,Jakóbisiak M




Has Abstract


1996-05-03 00:00:00














  • Interleukin-2-stimulated natural killer activity against malignant and benign endometrium.

    abstract::Natural cell-mediated immunity against autologous tumor cells, autologous endometrial epithelium, and allogeneic epidermoid carcinoma cell line HeLa was tested in 8 patients with endometrial carcinoma and one patient with endometrial stromal sarcoma. The average cytotoxicity of unstimulated peripheral blood lymphocyte...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Timonen T,Lehtovirta P,Saksela E

    更新日期:1987-10-15 00:00:00

  • Reduced allergy and immunoglobulin E among adults with intracranial meningioma compared to controls.

    abstract::Meningioma, the most frequent tumor in the central nervous system, has few recognized risk factors. We explored the role of allergies in a population-based case-control consortium study of meningioma in five geographic areas. We also studied serum levels of a marker of atopic allergy (IgE) in a subset of study partici...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Wiemels JL,Wrensch M,Sison JD,Zhou M,Bondy M,Calvocoressi L,Black PM,Yu H,Schildkraut JM,Claus EB

    更新日期:2011-10-15 00:00:00

  • Extracellular matrix and HIF-1 signaling: the role of prolidase.

    abstract::Hypoxia-inducible factor-1 (HIF-1) plays an important role in stress-responsive gene expression. Although primarily sensitive to hypoxia, HIF-1 signaling can be regulated by a number of stress factors including metabolic stress, growth factors and molecules present in the extracellular matrix (ECM). Degradation of ECM...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Surazynski A,Donald SP,Cooper SK,Whiteside MA,Salnikow K,Liu Y,Phang JM

    更新日期:2008-03-15 00:00:00

  • Human papillomavirus, herpes simplex virus and cervical cancer incidence in Greenland and Denmark. A population-based cross-sectional study.

    abstract::In Greenland, the incidence of cervical cancer is 5.7 times higher than in Denmark among women aged 20-39. From Nuuk (Greenland) and Nykøbing Falster (Denmark) a sample of 800 women aged 20-39 years was drawn at random. A total of 586 and 661 women were investigated in Greenland and Denmark respectively. All women had...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Kjaer SK,de Villiers EM,Haugaard BJ,Christensen RB,Teisen C,Møller KA,Poll P,Jensen H,Vestergaard BF,Lynge E

    更新日期:1988-04-15 00:00:00

  • Antagonism of chemokine receptor CXCR3 inhibits osteosarcoma metastasis to lungs.

    abstract::Metastasis continues to be the leading cause of mortality for patients with cancer. Several years ago, it became clear that chemokines and their receptors could control the tumor progress. CXCR3 has now been identified in many cancers including osteosarcoma and CXCR3 ligands were expressed by lungs that are the primar...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Pradelli E,Karimdjee-Soilihi B,Michiels JF,Ricci JE,Millet MA,Vandenbos F,Sullivan TJ,Collins TL,Johnson MG,Medina JC,Kleinerman ES,Schmid-Alliana A,Schmid-Antomarchi H

    更新日期:2009-12-01 00:00:00

  • Site-specific risk of cutaneous malignant melanoma and pattern of sun exposure in New Zealand.

    abstract::The site-specific relationship between melanoma and sun exposure was investigated in the high-risk region of New Zealand. Age and latitude of residence were used as biological and geographical proxy measures of exposure for the 16,117 newly incident cases and 3,150 death records reported between 1968 and 1993. Age-sta...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Bulliard JL

    更新日期:2000-03-01 00:00:00

  • Molecular cloning and characterization of endogenous SV40 DNA from human HBL-100 cells.

    abstract::The human HBL-100 cell line harbours SV40 DNA integrated in tandem at a unique site. The SV40 T-antigen expressed in these cells is defective in a function essential to the replication of the viral genome. The integrated SV40 sequences were molecularly cloned in a bacteriophage, and a subclone (plasmid pSVHBI) contain...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Saint-Ruf C,Nardeux P,Cebrian J,Lacasa M,Lavialle C,Cassingena R

    更新日期:1989-08-15 00:00:00

  • Human BLCAP transcript: new editing events in normal and cancerous tissues.

    abstract::Bladder cancer-associated protein (BLCAP) is a highly conserved protein among species, and it is considered a novel candidate tumor suppressor gene originally identified from human bladder carcinoma. However, little is known about the regulation or the function of this protein. Here, we show that the human BLCAP trans...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Galeano F,Leroy A,Rossetti C,Gromova I,Gautier P,Keegan LP,Massimi L,Di Rocco C,O'Connell MA,Gallo A

    更新日期:2010-07-01 00:00:00

  • Potentiation of tumor radiotherapy by a radiation-inducible oncolytic and oncoapoptotic adenovirus in cervical cancer xenografts.

    abstract::The p53 tumor suppressor pathway is impaired in more than 90% of cervical cancers and cancer-derived cell lines as a result of infection by human papillomavirus (HPV). The HPV E6 oncoprotein forms complexes with p53 and promotes its degradation via ubiquitin-dependent mechanism. In our study, we attempted to improve t...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Wang H,Song X,Zhang H,Zhang J,Shen X,Zhou Y,Fan X,Dai L,Qian G,Hoffman AR,Hu JF,Ge S

    更新日期:2012-01-15 00:00:00

  • Genome-wide profiling of normal gastric mucosa identifies Helicobacter pylori- and cancer-associated DNA methylome changes.

    abstract::The large geographic variations in the incidence of gastric cancer (GC) are likely due to differential environmental exposures, in particular to Helicobacter pylori (H. pylori) infection. We aimed to investigate the impact of H. pylori on the epigenome in normal gastric mucosa and methylation changes associated with c...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Woo HD,Fernandez-Jimenez N,Ghantous A,Degli Esposti D,Cuenin C,Cahais V,Choi IJ,Kim YI,Kim J,Herceg Z

    更新日期:2018-08-01 00:00:00

  • Expression of the ras-related rap genes in human tumors.

    abstract::The expression of the recently described rap genes, closely related in the effector region to the ras proto-oncogenes, was examined by Northern blot analysis in 41 primary human tumors. The structural and in vitro biological properties of the rap gene products suggest their possible antagonistic action in the same eff...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Culine S,Olofsson B,Gosselin S,Honore N,Tavitian A

    更新日期:1989-12-15 00:00:00

  • Hospicells derived from ovarian cancer stroma inhibit T-cell immune responses.

    abstract::With metastatic disease at diagnosis for 70% of patients, ovarian cancer represents the most lethal gynecological malignancy. Ovarian carcinomas are aggressive malignancies that can evade immune surveillance and frequently develop into metastases. The tumor microenvironment is decisive for preventing immune attack but...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Martinet L,Poupot R,Mirshahi P,Rafii A,Fournié JJ,Mirshahi M,Poupot M

    更新日期:2010-05-01 00:00:00

  • Rapamycin inhibits proliferation of human neuroblastoma cells without suppression of MycN.

    abstract::MYCN and insulin-like growth factor (IGF) system are important for the pathogenesis and development of neuroblastoma. We previously reported evidence of a direct linkage between MycN and the IGF system in KP-N-RT human neuroblastoma cells, where IGF-I induced both MycN expression at the RNA level and G1-S cell cycle p...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Misawa A,Hosoi H,Tsuchiya K,Sugimoto T

    更新日期:2003-03-20 00:00:00

  • Synergism of recombinant human interferon gamma with liposome-encapsulated muramyl tripeptide in activation of the tumoricidal properties of human monocytes.

    abstract::Freshly isolated human peripheral blood monocytes from healthy volunteers are not cytotoxic to allogeneic A375 melanoma cells, but they were rendered tumoricidal by incubation in vitro with either liposomes containing 5 micrograms/mumol phospholipid of muramyl tripeptide phosphatidylethanolamine (liposome-MTP-PE; opti...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Sone S,Tandon P,Utsugi T,Ogawara M,Shimizu E,Nii A,Ogura T

    更新日期:1986-10-15 00:00:00

  • Analysis of T-cell-receptor beta-chain-gene usage in peripheral-blood and tumor-infiltrating lymphocytes from human non-small-cell lung carcinomas.

    abstract::Non-small-cell lung cancers (NSCLC) are often infiltrated by T lymphocytes. It is postulated that the presence of tumor-infiltrating lymphocytes (TIL) reflects a local host immune response against autologous tumors. To identify the nature of NSCLC TIL, we have characterized the molecular structure of the TCRbeta chain...

    journal_title:International journal of cancer

    pub_type: 临床试验,杂志文章


    authors: Echchakir H,Asselin-Paturel C,Dorothee G,Vergnon I,Grunenwald D,Chouaib S,Mami-Chouaib F

    更新日期:1999-04-12 00:00:00

  • Sulfonated aluminium phthalocyanines as sensitizers for photochemotherapy. Effects of small light doses on localization, dye fluorescence and photosensitivity in V79 cells.

    abstract::V79 cells incubated with di- or tetrasulfonated aluminium phthalocyanines (AlPcS2 or AlPcS4) showed a granular fluorescence pattern. Co-staining with the lysosomotropic dye acridine orange (AO) indicated that the granules that were stained by these photoactive phthalocyanines were identical to lysosomes. Small light e...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Moan J,Berg K,Anholt H,Madslien K

    更新日期:1994-09-15 00:00:00

  • Cytoskeleton and ECM tumor mutant peptides: Increased protease sensitivities and potential consequences for the HLA class I mutant epitope reservoir.

    abstract::Cytoskeleton and extracellular matrix-related proteins (CECMPs) represent the most common class of cancer mutants, owing to the large size of their coding regions and the randomness of mutagenesis. We used a bioinformatics approach to assess the impact of amino acid (AA) substitutions on the sensitivity of CECMPs to p...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Callahan BM,Patel JS,Fawcett TJ,Blanck G

    更新日期:2018-03-01 00:00:00

  • Tumor-cell killing by MAbs against fucosyl GM1, a ganglioside antigen associated with small-cell lung carcinoma.

    abstract::Monoclonal antibodies (MAbs) reactive with the ganglioside fucosyl GM1 (Fuc-GM1), an antigen associated with small-cell carcinoma of the lung (SCLC), were tested for their ability to mediate tumor-cell killing in vitro in conjunction with humoral and cellular effectors and to inhibit tumor engraftment in nude mice in ...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Brezicka FT,Holmgren J,Kalies I,Lindholm L

    更新日期:1991-12-02 00:00:00

  • Germline mutations in E-cadherin do not explain association of hereditary prostate cancer, gastric cancer and breast cancer.

    abstract::Somatic mutations in the E-cadherin (CDH1) gene have frequently been reported in cases with diffuse gastric and lobular breast cancers. Recently, germline mutations have been identified in families with diffuse gastric cancers. In families with hereditary prostate cancer (HPC), a significant association of prostate ca...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Jonsson BA,Bergh A,Stattin P,Emmanuelsson M,Grönberg H

    更新日期:2002-04-20 00:00:00

  • The mechanism of hepatic uptake of a radiolabelled monoclonal antibody.

    abstract::Clinical and experimental scintigraphic studies have found that radiolabelled antibodies are not only taken up by tumour(s) but also by normal liver. The accumulation of radionuclides in this organ poses a major problem to the use of radiolabelled antibodies as diagnostic and therapeutic tools. In an attempt to unders...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Boyle CC,Paine AJ,Mather SJ

    更新日期:1992-04-01 00:00:00

  • Cohort study of thyroid cancer in a San Francisco Bay area population.

    abstract::Using data from a large health plan, we performed a cohort study of thyroid cancer among 204,964 persons (aged 10--89 at baseline in 1964--1973, 54% female) followed for a median of 20 years. There were 196 incident thyroid cancers (73 in men, 123 in women). Risk was independently and positively related to female gend...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Iribarren C,Haselkorn T,Tekawa IS,Friedman GD

    更新日期:2001-09-01 00:00:00

  • CpG island methylator phenotype association with upregulated telomerase activity in hepatocellular carcinoma.

    abstract::CpG island methylator phenotype (CIMP) involves the targeting of multiple genes by promoter hypermethylation. Telomerase plays an important role in the development of cellular immortality and oncogenesis. To gain insight into the role of epigenetic aberration of telomerase-related genes in hepatocarcinogenesis, we det...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Zhang C,Guo X,Jiang G,Zhang L,Yang Y,Shen F,Wu M,Wei L

    更新日期:2008-09-01 00:00:00

  • Placental bone morphogenetic protein (PLAB) gene expression in normal, pre-malignant and malignant human prostate: relation to tumor development and progression.

    abstract::The second most common target of prostate-cancer metastasis is bone, and the phenomenon of skeletal metastasis represents the incurable stage of disease. Histologically, skeletal metastasis from prostate cancer is distinctive due to its osteoblastic nature. The osteoblastic bone metastasis shows extensive new bone for...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Thomas R,True LD,Lange PH,Vessella RL

    更新日期:2001-07-01 00:00:00

  • Lymphangiogenesis in colorectal cancer--prognostic and therapeutic aspects.

    abstract::Colorectal cancer (CRC) represents the second most common cause of cancer mortality in the western world. The tumors frequently show metastatic spread which affects different organs such as lymph nodes, liver and lungs. Although the pattern of spread may vary, the initial step usually involves dissemination to regiona...

    journal_title:International journal of cancer

    pub_type: 杂志文章,评审


    authors: Sundlisaeter E,Dicko A,Sakariassen PØ,Sondenaa K,Enger PØ,Bjerkvig R

    更新日期:2007-10-01 00:00:00

  • Impact of an HPV6/11/16/18 L1 virus-like particle vaccine on progression to cervical intraepithelial neoplasia in seropositive women with HPV16/18 infection.

    abstract::The impact of a human papillomavirus (HPV) vaccine on development of cervical intraepithelial neoplasia grade 2-3 or adenocarcinoma in situ (CIN2-3/AIS) in women with ongoing HPV16 or 18 infections prevaccination is reported. Seventeen thousand six-hundred and twenty-two women aged 16-26 were enrolled in 1 of 2 random...

    journal_title:International journal of cancer

    pub_type: 杂志文章,随机对照试验


    authors: Haupt RM,Wheeler CM,Brown DR,Garland SM,Ferris DG,Paavonen JA,Lehtinen MO,Steben M,Joura EA,Giacoletti KE,Radley DR,James MK,Saah AJ,Sings HL,FUTURE I and II Investigators.

    更新日期:2011-12-01 00:00:00

  • RPR-115135, a farnesyltransferase inhibitor, increases 5-FU- cytotoxicity in ten human colon cancer cell lines: role of p53.

    abstract::A new non peptidic farnesyltransferase inhibitor, RPR-115135, in combination with 5-FU was studied in 10 human colon cancer cell lines (HCT-116, RKO, DLD-1, Colo-320, LoVo, SW-620, HT-29, HCT-15, Colo-205 and KM-12) carrying several mutations but well characterized for p53 and Ras status. We found that there was a sli...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Russo P,Malacarne D,Falugi C,Trombino S,O'Connor PM

    更新日期:2002-07-20 00:00:00

  • Frequent promoter hypermethylation of Wnt pathway inhibitor genes in malignant astrocytic gliomas.

    abstract::Aberrant activation of wingless (Wnt) signaling is involved in the pathogenesis of various cancers. Recent studies suggested a role of Wnt signaling in gliomas, the most common primary brain tumors. We investigated 70 gliomas of different malignancy grades for promoter hypermethylation in 8 genes encoding members of t...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Götze S,Wolter M,Reifenberger G,Müller O,Sievers S

    更新日期:2010-06-01 00:00:00

  • Neuropilin-2 and its ligand VEGF-C predict treatment response after transurethral resection and radiochemotherapy in bladder cancer patients.

    abstract::The standard treatment for invasive bladder cancer is radical cystectomy. In selected patients, bladder-sparing therapy can be performed by transurethral resection (TURBT) and radio-chemotherapy (RCT) or radiotherapy (RT). Our published in vitro data suggest that the Neuropilin-2 (NRP2)/VEGF-C axis plays a role in the...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Keck B,Wach S,Taubert H,Zeiler S,Ott OJ,Kunath F,Hartmann A,Bertz S,Weiss C,Hönscheid P,Schellenburg S,Rödel C,Baretton GB,Sauer R,Fietkau R,Wullich B,Krause FS,Datta K,Muders MH

    更新日期:2015-01-15 00:00:00

  • Overexpression of the polarity protein PAR-3 in clear cell renal cell carcinoma is associated with poor prognosis.

    abstract::The partition-defective 3 (PAR-3) protein is implicated in the development and maintenance of cell polarity and is associated with proteins that mediate the changes in cytoskeleton organization required for cell polarity establishment. In this work, we used two original primary cell lines (R-180 and R-305) derived fro...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Dugay F,Le Goff X,Rioux-Leclerq N,Chesnel F,Jouan F,Henry C,Cabillic F,Verhoest G,Vigneau C,Arlot-Bonnemains Y,Belaud-Rotureau MA

    更新日期:2014-05-01 00:00:00

  • Genes and molecular pathways related to radioresistance of oral squamous cell carcinoma cells.

    abstract::To identify genes associated with radioresistant oral squamous cell carcinoma (OSCC), we compared gene expression signatures between OSCC cell lines exhibiting radioresistance and cells with radiosensitivity after X-ray irradiation in a dose-dependent manner using Affymetrix GeneChip analysis with Human Genome-U133 pl...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Ishigami T,Uzawa K,Higo M,Nomura H,Saito K,Kato Y,Nakashima D,Shiiba M,Bukawa H,Yokoe H,Kawata T,Ito H,Tanzawa H

    更新日期:2007-05-15 00:00:00