A factor from Trypanosoma cruzi induces repetitive cytosolic free Ca2+ transients in isolated primary canine cardiac myocytes.


:An unusual 120-kDa alkaline peptidase contained in a trypomastigote soluble fraction (TSF) of Trypanosoma cruzi is associated with the induction of repetitive Ca2+ transients and subsequent invasion by the parasite of a number of mammalian cell lines, including tissue culture L6E2 myoblasts (B. A. Burleigh and N. W. Andrews, J. Biol. Chem. 270:5172-5180, 1995; S. N. J. Moreno, J. Silva, A. E. Vercesi, and R. Docampo, J. Exp. Med. 180:1535-1540, 1994; A. Rodríguez, M. G. Rioult, A. Ora, and N. W. Andrews, J. Cell Biol. 129:1263-1273, 1995; I. Tardieux, M. H. Nathanson, and N. W. Andrews, J. Exp. Med. 179:1017-1022, 1994). Using single cell spectrofluorometry and whole-cell patch clamping, we show that TSF produces rapid repetitive cytosolic Ca2+ transients (each associated with cell contraction) in primary cardiac myocytes isolated from dogs. The response of myocytes to TSF was dose dependent in that increasing numbers of cells responded to increasing concentrations of TSF. The TSF-induced Ca2+ transients could be obliterated when TSF was heated or treated with trypsin or the protease inhibitor leupeptin. Aprotinin, pepstatin A, and E-64 did not affect TSF activity. The TSF-induced Ca2+ transients and trypomastigote cell invasion could not be inhibited by alpha (prazosin)- or beta (propanolol)-adrenergic blockers or L-type Ca2+ channel blockers (verapamil, nisoldipine, or cadmium) or by removal of extracellular Ca2+. However, inhibition of pertussis toxin-sensitive G proteins and Ca2+ release from the sarcoplasmic reticulum (with thapsigargin or ryanodine) prevented the TSF-induced Ca2+ transients and cell invasion by trypomastigotes. These data suggested that cardiac myocyte pertussis toxin-sensitive G proteins are associated with the regulation of TSF-induced Ca2+ transients and myocyte invasion by trypomastigotes but are independent of Ca2+ entry into the cytosol via L-type Ca2+ channels. The Ca2+ transients are dependent on release of Ca2+ from sarcoplasmic reticulum Ca2+ stores, but this release is not dependent on extracellular Ca2+ or on the classic model of Ca2+ -induced Ca2+ release in cardiac myocytes. Further, subthreshold depolarizations, together with cell contraction as demonstrated by whole-cell patch clamping, occurred with each Ca2+ transient. However, the depolarizations were of magnitude insufficient to generate an action potential, providing further evidence for a lack of dependence on L-type Ca2+ channels and other voltage-dependent channels (Na+ and K+ channels) in the generation of TSF-induced Ca2+ transients. Our findings suggest that primary canine cardiac myocytes respond to TSF and parasite invasion in ways similar to those of the in vitro cell lines studied to date. Since cardiac myocytes are primary targets for T. cruzi in the vertebrate host, our study indicates that TSF may play a role in the pathogenesis of Chagas' disease in humans.


Infect Immun


Infection and immunity


Barr SC,Han W,Andrews NW,Lopez JW,Ball BA,Pannabecker TL,Gilmour RF Jr




Has Abstract


1996-05-01 00:00:00












  • Mitogenic stimulation of murine spleen cells: relation to susceptibility to Salmonella infection.

    abstract::The screening of several inbred strains of mice suggested that the capacity of their spleen cells to respond to the mitogenic effect of lipopolysaccharide (LPS) of gram-negative bacteria was correlated with their resistance to intraperitoneal infection with Salmonella typhimurium. An infection of LPS into mice caused ...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: von Jeney N,Günther E,Jann K

    更新日期:1977-01-01 00:00:00

  • Adhesion-dependent activation of mucosal interleukin-6 production.

    abstract::Mucosal exposure to Escherichia coli elicits an inflammatory response in the urinary tract. Interleukin-6 (IL-6) is secreted into the urine, and polymorphonuclear leukocytes (PMNLs) are recruited to the site of infection. This study analyzed the ability of mucosally administered bacterial components to activate IL-6 a...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Linder H,Engberg I,Hoschützky H,Mattsby-Baltzer I,Svanborg C

    更新日期:1991-12-01 00:00:00

  • Initial serum antibody titer to Porphyromonas gingivalis influences development of antibody avidity and success of therapy for chronic periodontitis.

    abstract::This study assessed the effect of periodontal therapy on specific serum antibody concentration, expressed as titer, and antibody binding strength, expressed as relative avidity. The immune responses to Porphyromonas gingivalis and Actinobacillus actinomycetemcomitans were investigated. Antibody titer was assayed by en...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Mooney J,Adonogianaki E,Riggio MP,Takahashi K,Haerian A,Kinane DF

    更新日期:1995-09-01 00:00:00

  • Chronic proliferative arthritis of mice induced by Mycoplasma arthritidis. II. Serological responses of the lost and effect of vaccines.

    abstract::Complement-fixing antibodies were first detected in mice 7 days after intravenous injection with Mycoplasma arthritidis. Peak titers were observed at 21 days, and high levels of antibody persisted through 293 days. The metabolism-inhibiting antibody response was minimal. On fractionation of mouse sera, only 7S antibod...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Coke BC,Ward JR,Golightly-Rowland C,Trapp GA

    更新日期:1971-10-01 00:00:00

  • Caspase and bid involvement in Clostridium difficile toxin A-induced apoptosis and modulation of toxin A effects by glutamine and alanyl-glutamine in vivo and in vitro.

    abstract::Clostridium difficile is the leading cause of nosocomial bacterial diarrhea. Glutamine and its stable and highly soluble derivative alanyl-glutamine, have been beneficial in models of intestinal injury. In this study, we extend our work on the mechanisms of Clostridium difficile toxin A (TxA)-induced apoptosis in huma...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Carneiro BA,Fujii J,Brito GA,Alcantara C,Oriá RB,Lima AA,Obrig T,Guerrant RL

    更新日期:2006-01-01 00:00:00

  • Digital Design of a Universal Rat Intraoral Device for Therapeutic Evaluation of a Topical Formulation against Candida-Associated Denture Stomatitis.

    abstract::Candida-associated denture stomatitis (DS) is a persistent and chronic oral infection of the denture-bearing palatal mucosa. DS stems from the ability of the fungal opportunistic pathogen Candida albicans to adhere to denture material and invade palatal tissue. Although DS is the most prevalent form of oral candidiasi...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Sultan AS,Rizk AM,Vila T,Ji Y,Masri R,Jabra-Rizk MA

    更新日期:2019-11-18 00:00:00

  • Immunogenicity of Salmonella typhi Ty21a vaccine for young children.

    abstract::An attenuated Salmonella typhi Ty21a vaccine was administered to 18 infants and toddlers (less than or equal to 24 months old) to determine its safety and immunogenicity. The vaccination (10(9) CFU per dose, three doses) was well tolerated. However, after the vaccination there was no evidence of a humoral or cellular ...

    journal_title:Infection and immunity

    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Murphy JR,Grez L,Schlesinger L,Ferreccio C,Baqar S,Muñoz C,Wasserman SS,Losonsky G,Olson JG,Levine MM

    更新日期:1991-11-01 00:00:00

  • Staphylococcus aureus Lipoic Acid Synthesis Limits Macrophage Reactive Oxygen and Nitrogen Species Production To Promote Survival during Infection.

    abstract::Macrophages are critical mediators of innate immunity and must be overcome for bacterial pathogens to cause disease. The Gram-positive bacterium Staphylococcus aureus produces virulence factors that impede macrophages and other immune cells. We previously determined that production of the metabolic cofactor lipoic aci...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Grayczyk JP,Alonzo F 3rd

    更新日期:2019-09-19 00:00:00

  • Enhanced expression of interleukin-1alpha and tumor necrosis factor receptor-associated protein 1 in ileal tissues of cattle infected with Mycobacterium avium subsp. paratuberculosis.

    abstract::Infection with Mycobacterium avium subsp. paratuberculosis is associated with high levels of morbidity, decreased production, and early culling in dairy cattle. Clinical symptoms of Johne's disease include persistent diarrhea, inappetence, and resultant weight loss due to chronic inflammation of the small intestine. A...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Aho AD,McNulty AM,Coussens PM

    更新日期:2003-11-01 00:00:00

  • Identification of multilocus genetic heterogeneity in Anaplasma marginale subsp. centrale and its restriction following tick-borne transmission.

    abstract::Anaplasma marginale subsp. centrale was the first vaccine used to protect against a rickettsial disease and is still in widespread use a century later. As its use preceded development of either cryopreservation or cell culture, the vaccine strain was maintained for decades by sequential passage among donor animals, ex...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Herndon DR,Ueti MW,Reif KE,Noh SM,Brayton KA,Agnes JT,Palmer GH

    更新日期:2013-05-01 00:00:00

  • Conditions for production, and some characteristics, of mycobacterial growth inhibitory factor produced by spleen cells from mice immunized with viable cells of the attenuated H37Ra strain of Mycobacterium tuberculosis.

    abstract::Mycobacterial growth inhibitory factor (MycoIF), found in supernatant fluids of mouse spleen cell cultures that have been stimulated in vitro with homologous antigen, inhibited the intracellular multiplication of virulent tubercle bacilli within normal mouse peritoneal macrophages in vitro. Antigenically stimulated H...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Cahall DL,Youmans GP

    更新日期:1975-10-01 00:00:00

  • Lipopolysaccharide structure determines ionic and hydrophobic binding of a cationic antimicrobial neutrophil granule protein.

    abstract::Bactericidal activity and binding of a 57,000-dalton cationic antimicrobial neutrophil granule protein (CAP57) are determined by the presence on bacteria of O-antigen polysaccharide chains and the availability of negatively charged groups in the lipid A region, the inner core region, or both regions of lipopolysacchar...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Farley MM,Shafer WM,Spitznagel JK

    更新日期:1988-06-01 00:00:00

  • Nonimmune binding of human immunoglobulin A (IgA) and IgG Fc by distinct sequence segments of the EibF cell surface protein of Escherichia coli.

    abstract::The eib genes of Escherichia coli encode surface-exposed proteins which bind immunoglobulins (Ig) such as the Fc fragment of human IgG (IgG Fc) in a nonimmune manner. The Eib proteins belong to a family which includes YadA of Yersinia, UspA2 of Moraxella, and DsrA of Haemophilus ducreyi. This family of surface-exposed...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Sandt CH,Hill CW

    更新日期:2001-12-01 00:00:00

  • Murine malaria: immune complexes inhibit Fc receptor-mediated phagocytosis.

    abstract::Immune complexes have been partially purified from the serum of Plasmodium berghei-infected mice by ultracentrifugation on 10 to 40% linear sucrose gradients, by precipitation with polyethylene glycol, and by gel filtration through Sephacryl S-300. The complexes contain gamma 1, gamma 2a, gamma 2b, and gamma 3 subclas...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Shear HL

    更新日期:1984-04-01 00:00:00

  • Prevention of C3 deposition by capsular polysaccharide is a virulence mechanism of type III group B streptococci.

    abstract::Strains of type III group B streptococci isolated from patients with neonatal sepsis are generally resistant to complement-mediated phagocytic killing in the absence of specific antibody. It has been suggested that the resistance of type III group B streptococci to phagocytosis results from inhibition of alternative-c...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Marques MB,Kasper DL,Pangburn MK,Wessels MR

    更新日期:1992-10-01 00:00:00

  • Humoral and cellular responses of mice to infection with a cold-adapted influenza A virus variant.

    abstract::The serum antibody response and four different cellular immune responses (cytotoxic T cells, delayed-type hypersensitivity T cells, natural killer cells, and cytotoxic macrophage levels) induced in CBA/H mice were measured at different times after intranasal inoculation of a cold-adapted (ca) variant of influenza A vi...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Mak NK,Zhang YH,Ada GL,Tannock GA

    更新日期:1982-10-01 00:00:00

  • Lack of antibacterial activity after intravenous hydrogen peroxide infusion in experimental Escherichia coli sepsis.

    abstract::The intravenous administration of hydrogen peroxide has been reported to benefit patients with pneumonia and to reduce Plasmodium parasitemia in experimentally infected mice. We assessed the antibacterial activity of intravenously infused hydrogen peroxide against hydrogen peroxide-susceptible Escherichia coli (MBC of...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Shenep JL,Stokes DC,Hughes WT

    更新日期:1985-06-01 00:00:00

  • The Yersinia enterocolitica GsrA stress protein, involved in intracellular survival, is induced by macrophage phagocytosis.

    abstract::The Yersinia enterocolitica gsrA gene is a stress protein gene which was originally identified as essential for protecting cells under both extracellular environmental stress and intracellular stress in macrophages due to phagocytosis. The gsrA gene was shown to be a member of the htrA class of genes and to possess a ...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Yamamoto T,Hanawa T,Ogata S,Kamiya S

    更新日期:1997-06-01 00:00:00

  • Effect of oral immunization with recombinant urease on murine Helicobacter felis gastritis.

    abstract::The ability of oral immunization to interfere with the establishment of infection with Helicobacter felis was examined. Groups of Swiss Webster mice were immunized orally with 250 micrograms of Helicobacter pylori recombinant urease (rUrease) and 10 micrograms of cholera toxin (CT) adjuvant, 1 mg of H. felis sonicate ...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Pappo J,Thomas WD Jr,Kabok Z,Taylor NS,Murphy JC,Fox JG

    更新日期:1995-04-01 00:00:00

  • Mycobacterium marinum Erp is a virulence determinant required for cell wall integrity and intracellular survival.

    abstract::The Mycobacterium tuberculosis exported repetitive protein (Erp) is a virulence determinant required for growth in cultured macrophages and in vivo. To better understand the role of Erp in Mycobacterium pathogenesis, we generated a mutation in the erp homologue of Mycobacterium marinum, a close genetic relative of M. ...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Cosma CL,Klein K,Kim R,Beery D,Ramakrishnan L

    更新日期:2006-06-01 00:00:00

  • Inhibition of bactericidal activity of anticapsular antibody by nonspecific antibodies reactive with surface-exposed antigenic determinants on Actinobacillus pleuropneumoniae.

    abstract::In an attempt to understand the mechanism of serum resistance in Actinobacillus pleuropneumoniae, in the present study we examined various interactions among the bacterial surface constituents, serum antibodies, and complement. Analysis of swine sera revealed the presence of anticapsular antibodies in convalescent-pha...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Udeze FA,Kadis S

    更新日期:1992-09-01 00:00:00

  • Partial characterization of an external polysaccharide of Helicobacter pylori by using an immunoglobulin M monoclonal antibody.

    abstract::A monoclonal immunoglobulin M antibody, HP15/36, was produced by a hybridoma cell line prepared by fusion of mouse myeloma cell line Sp2/O with spleen cells of mice immunized with Helicobacter pylori D273 (French strain). Immunoelectron microscopy of whole bacteria and ultrathin sections showed that the determinant wa...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Drouet EB,De Montclos HP,Andujar M,Boude M,Grimaud JA,Denoyel GA

    更新日期:1993-06-01 00:00:00

  • Expression of cathelicidin LL-37 during Mycobacterium tuberculosis infection in human alveolar macrophages, monocytes, neutrophils, and epithelial cells.

    abstract::The innate immune response in human tuberculosis is not completely understood. To improve our knowledge regarding the role of cathelicidin hCAP-18/LL37 in the innate immune response to tuberculosis infection, we used immunohistochemistry, immunoelectron microscopy, and gene expression to study the induction and produc...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Rivas-Santiago B,Hernandez-Pando R,Carranza C,Juarez E,Contreras JL,Aguilar-Leon D,Torres M,Sada E

    更新日期:2008-03-01 00:00:00

  • Opacity-associated adhesin repertoire in hyperinvasive Neisseria meningitidis.

    abstract::The opacity (Opa) proteins mediate a variety of interactions between the bacterium Neisseria meningitidis and its human host. These interactions are thought to be of central importance in both the asymptomatic colonization of the nasopharynx and the sporadic occurrence of meningococcal disease. The receptor specificit...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Callaghan MJ,Jolley KA,Maiden MC

    更新日期:2006-09-01 00:00:00

  • Reactivation of polyoma virus in kidneys of persistently infected mice during pregnancy.

    abstract::Female mice infected at birth with 10(7) 50% tissue culture infective doses of polyoma virus were mated when at least 6 weeks old. Polyoma was not detected in any tissues of 27 female mice before mating except for trace amounts in the kidneys of 2 mice, but late in gestation polyoma virus could be found in the kidneys...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: McCance DJ,Mims CA

    更新日期:1979-09-01 00:00:00

  • Generation of Trypanosoma cruzi-specific CD8+ T-cell immunity is unaffected by the absence of type I interferon signaling.

    abstract::Trypanosoma cruzi is a protozoan parasite that causes human Chagas' disease, a leading source of congestive heart failure in Central and South America. CD8+ T cells are critical for control of T. cruzi infection, and CD8+ T cells recognizing the immunodominant trans-sialidase gene-encoded peptide TSKB20 (ANYKFTLV) acc...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Martin DL,Murali-Krishna K,Tarleton RL

    更新日期:2010-07-01 00:00:00

  • Improved tuberculosis DNA vaccines by formulation in cationic lipids.

    abstract::Mice were vaccinated with plasmid DNA (pDNA) encoding antigen 85A (Ag85A), Ag85B, or PstS-3 from Mycobacterium tuberculosis either in saline or formulated for intramuscular injections in VC1052:DPyPE (aminopropyl-dimethyl-myristoleyloxy-propanaminium bromide-diphytanoylphosphatidyl-ethanolamine) (Vaxfectin; Vical, Inc...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: D'Souza S,Rosseels V,Denis O,Tanghe A,De Smet N,Jurion F,Palfliet K,Castiglioni N,Vanonckelen A,Wheeler C,Huygen K

    更新日期:2002-07-01 00:00:00

  • Hepatitis B surface antigen-specific cell-mediated immune responses in human chronic hepatitis B surface antigen carriers.

    abstract::The presence of hepatitis B surface antigen (HBsAg) and antibody (anti-HBs), hepatitis B e antigen (HBeAg) and antibody (anti-HBe), the nature of T-cell function, and specific cell-mediated immunity to HBsAg were determined and evaluated serially in groups of subjects with chronic HBsAg carrier states and in seronegat...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Beutner KR,Tiku ML,Ogra PL

    更新日期:1978-08-01 00:00:00

  • Role of flm locus in mesophilic Aeromonas species adherence.

    abstract::The adherence mechanism of Aeromonas caviae Sch3N to HEp-2 cells was initially investigated through four mini-Tn5 mutants that showed a 10-fold decrease in adherence. These mutants lost motility, flagella, and their lipopolysaccharide (LPS) O antigen (O-Ag). Three genes, flmB-neuA-flmD, were found to be interrupted by...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Gryllos I,Shaw JG,Gavín R,Merino S,Tomás JM

    更新日期:2001-01-01 00:00:00

  • Biofilm formation by Moraxella catarrhalis in vitro: roles of the UspA1 adhesin and the Hag hemagglutinin.

    abstract::Mutant analysis was used to identify Moraxella catarrhalis gene products necessary for biofilm development in a crystal violet-based assay involving 24-well tissue culture plates. The wild-type M. catarrhalis strains that formed the most extensive biofilms in this system proved to be refractory to transposon mutagenes...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Pearson MM,Laurence CA,Guinn SE,Hansen EJ

    更新日期:2006-03-01 00:00:00