A factor from Trypanosoma cruzi induces repetitive cytosolic free Ca2+ transients in isolated primary canine cardiac myocytes.


:An unusual 120-kDa alkaline peptidase contained in a trypomastigote soluble fraction (TSF) of Trypanosoma cruzi is associated with the induction of repetitive Ca2+ transients and subsequent invasion by the parasite of a number of mammalian cell lines, including tissue culture L6E2 myoblasts (B. A. Burleigh and N. W. Andrews, J. Biol. Chem. 270:5172-5180, 1995; S. N. J. Moreno, J. Silva, A. E. Vercesi, and R. Docampo, J. Exp. Med. 180:1535-1540, 1994; A. Rodríguez, M. G. Rioult, A. Ora, and N. W. Andrews, J. Cell Biol. 129:1263-1273, 1995; I. Tardieux, M. H. Nathanson, and N. W. Andrews, J. Exp. Med. 179:1017-1022, 1994). Using single cell spectrofluorometry and whole-cell patch clamping, we show that TSF produces rapid repetitive cytosolic Ca2+ transients (each associated with cell contraction) in primary cardiac myocytes isolated from dogs. The response of myocytes to TSF was dose dependent in that increasing numbers of cells responded to increasing concentrations of TSF. The TSF-induced Ca2+ transients could be obliterated when TSF was heated or treated with trypsin or the protease inhibitor leupeptin. Aprotinin, pepstatin A, and E-64 did not affect TSF activity. The TSF-induced Ca2+ transients and trypomastigote cell invasion could not be inhibited by alpha (prazosin)- or beta (propanolol)-adrenergic blockers or L-type Ca2+ channel blockers (verapamil, nisoldipine, or cadmium) or by removal of extracellular Ca2+. However, inhibition of pertussis toxin-sensitive G proteins and Ca2+ release from the sarcoplasmic reticulum (with thapsigargin or ryanodine) prevented the TSF-induced Ca2+ transients and cell invasion by trypomastigotes. These data suggested that cardiac myocyte pertussis toxin-sensitive G proteins are associated with the regulation of TSF-induced Ca2+ transients and myocyte invasion by trypomastigotes but are independent of Ca2+ entry into the cytosol via L-type Ca2+ channels. The Ca2+ transients are dependent on release of Ca2+ from sarcoplasmic reticulum Ca2+ stores, but this release is not dependent on extracellular Ca2+ or on the classic model of Ca2+ -induced Ca2+ release in cardiac myocytes. Further, subthreshold depolarizations, together with cell contraction as demonstrated by whole-cell patch clamping, occurred with each Ca2+ transient. However, the depolarizations were of magnitude insufficient to generate an action potential, providing further evidence for a lack of dependence on L-type Ca2+ channels and other voltage-dependent channels (Na+ and K+ channels) in the generation of TSF-induced Ca2+ transients. Our findings suggest that primary canine cardiac myocytes respond to TSF and parasite invasion in ways similar to those of the in vitro cell lines studied to date. Since cardiac myocytes are primary targets for T. cruzi in the vertebrate host, our study indicates that TSF may play a role in the pathogenesis of Chagas' disease in humans.


Infect Immun


Infection and immunity


Barr SC,Han W,Andrews NW,Lopez JW,Ball BA,Pannabecker TL,Gilmour RF Jr




Has Abstract


1996-05-01 00:00:00












  • Production of stabilized virulence factor-negative variants by group A streptococci during stationary phase.

    abstract::Many of the virulence factors associated with fulminant group A streptococci (GAS) infection are expressed under in vitro exponential growth conditions. However, the survival of GAS in tissue and intracellularly, as well as colonization of asymptomatic carriers, has been reported for GAS. The bacteria associated with ...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Leonard BA,Woischnik M,Podbielski A

    更新日期:1998-08-01 00:00:00

  • alpha(v)beta(3) integrin and bacterial lipopolysaccharide are involved in Coxiella burnetii-stimulated production of tumor necrosis factor by human monocytes.

    abstract::Coxiella burnetii, the agent of Q fever, enters human monocytes through alpha(v)beta(3) integrin and survives inside host cells. In addition, C. burnetii stimulates the synthesis of inflammatory cytokines including tumor necrosis factor (TNF) by monocytes. We studied the role of the interaction of C. burnetii with THP...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Dellacasagrande J,Ghigo E,Hammami SM,Toman R,Raoult D,Capo C,Mege JL

    更新日期:2000-10-01 00:00:00

  • OxyR acts as a repressor of catalase expression in Neisseria gonorrhoeae.

    abstract::It has been reported that Neisseria gonorrhoeae possesses a very high level of catalase activity, but the regulation of catalase expression has not been investigated extensively. In Escherichia coli and Salmonella enterica serovar Typhimurium, it has been demonstrated that OxyR is a positive regulator of hydrogen pero...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Tseng HJ,McEwan AG,Apicella MA,Jennings MP

    更新日期:2003-01-01 00:00:00

  • Inactivation of the hemagglutinins of type A influenza viruses by physical and chemical means: an aid to classification.

    abstract::The effects of temperature and treatment with sodium dodecyl sulfate, Tween 20, dithiothreitol, trypsin, or guanidine on the hemagglutinating capacity of six strains of type A influenza virus (A(0)/PR8/34, A(1)/CAME/46, A(2)/J305/57, A(2)/Bethesda/63, A(2)/HK/Aichi/68, and A(2)/HK/80/68), one strain of swine virus (A/...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Cleeland R,Grunberg E,Prince HN

    更新日期:1972-03-01 00:00:00

  • Duplicate copies of lic1 direct the addition of multiple phosphocholine residues in the lipopolysaccharide of Haemophilus influenzae.

    abstract::The genes of the lic1 operon (lic1A to lic1D) are responsible for incorporation of phosphocholine (PCho) into the lipopolysaccharide (LPS) of Haemophilus influenzae. PCho plays a multifaceted role in the commensal and pathogenic lifestyles of a range of mucosal pathogens, including H. influenzae. Structural studies of...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Fox KL,Li J,Schweda EK,Vitiazeva V,Makepeace K,Jennings MP,Moxon ER,Hood DW

    更新日期:2008-02-01 00:00:00

  • Distribution of indigenous bacteria in the digestive tract of conventional and gnotobiotic rats.

    abstract::The localization and population levels of the indigenous bacterial flora of conventional rats were investigated by cultural and histological techniques. Lactobacilli predominate in the stomach and upper part of the small intestine and associate with keratinized cells of the nonglandular portion of stomach. Mixtures of...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Morotomi M,Watanabe T,Suegara N,Kawai Y,Mutai M

    更新日期:1975-05-01 00:00:00

  • Inhibition of T-cell response by Escherichia coli heat-labile enterotoxin-treated epithelial cells.

    abstract::Escherichia coli heat-labile enterotoxin (LT) is an extensively studied adjuvant of mucosal responses. Nevertheless, its mode of action as an adjuvant remains incompletely understood. In this study, we describe a simplified in vitro model with which to look at some aspects of immunoregulation by LT. The interaction of...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Lopes LM,Maroof A,Dougan G,Chain BM

    更新日期:2000-12-01 00:00:00

  • Candida albicans and Candida stellatoidea, in contrast to other Candida species, bind iC3b and C3d but not C3b.

    abstract::It was demonstrated that complement-coated sheep erythrocytes bind to Candida albicans cells grown in serum-free RPMI 1640 medium. Testing of purified complement components proved that iC3b and C3d were responsible for the reaction, whereas C3b and C3b-H reacted only slightly if at all. Binding occurred only to C. alb...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Heidenreich F,Dierich MP

    更新日期:1985-11-01 00:00:00

  • Comparison of protection induced by immunization with recombinant proteins from different regions of merozoite surface protein 1 of Plasmodium yoelii.

    abstract::Vaccination with native full-length merozoite surface protein 1 (MSP1) or with recombinant C-terminal peptides protects mice against lethal challenge with virulent malaria parasites. To determine whether other regions of MSP1 can also induce protection, Plasmodium yoelii MSP1 was divided into four separate regions. Ea...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Tian JH,Kumar S,Kaslow DC,Miller LH

    更新日期:1997-08-01 00:00:00

  • Effect of chemical modification of histidine and tyrosine residues in toxic shock syndrome toxin 1 on the serologic and mitogenic activities of the toxin.

    abstract::Modification of three or four of the five histidine residues in the toxic shock syndrome toxin 1 (TSST-1) with diethylpyrocarbonate did not inhibit the precipitin reaction of the modified TSST-1 with polyvalent antisera to the toxin. Monoclonal antibody 7T did not react with the modified TSST-1, but monoclonal antibod...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Kokan-Moore NP,Bergdoll MS

    更新日期:1989-07-01 00:00:00

  • Phorbol esters enhance the cyclic GMP response of T84 cells to the heat-stable enterotoxin of Escherichia coli (STa).

    abstract::We examined the effect of protein kinase C (PKC) activation on the cyclic GMP response to heat-stable enterotoxin (STa) in a colonic carcinoma intestinal epithelial cell line, T84 cells. Our results demonstrate that the active phorbol ester analog, phorbol dibutyrate, but not the inactive alpha-phorbol dibutyrate, act...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Weikel CS,Spann CL,Chambers CP,Crane JK,Linden J,Hewlett EL

    更新日期:1990-05-01 00:00:00

  • Identification of a surrogate marker for infection in the African green monkey model of inhalation anthrax.

    abstract::In 2001, a bioterrorism attack involving Bacillus anthracis spore-laced letters resulted in 22 cases of inhalation anthrax, with five fatalities. This incident identified gaps in our health care system and precipitated a renewed interest in identifying both therapeutics and rapid diagnostic assays. To address those ga...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Rossi CA,Ulrich M,Norris S,Reed DS,Pitt LM,Leffel EK

    更新日期:2008-12-01 00:00:00

  • Effect of oxygen-dependent antimicrobial systems on Legionella pneumophila.

    abstract::Legionella pneumophila was susceptible to the antimicrobial action of oxygen metabolites generated by both the myeloperoxidase-H(2)O(2)-halide and the xanthine oxidase systems. ...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Lochner JE,Friedman RL,Bigley RH,Iglewski BH

    更新日期:1983-01-01 00:00:00

  • Effect of temperature on survival and growth of infectious pancreatic necrosis virus.

    abstract::Infectious pancreatic necrosis virus was stable for 10 days at 4 C in stream and well water, after which the virus had a half-life of 7.5 days. At 15 C, the virus was stable for 5 days, and then had a half-life between 5 and 6 days. Viral antigen in infected cells developed much more slowly at 4 C than at 20 C. Infect...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Tu KC,Spendlove RS,Goede RW

    更新日期:1975-06-01 00:00:00

  • Local and systemic antibody response to oral administration of glucosyltransferase antigen complex.

    abstract::The salivary and serum immune responses to orally administered glucosyltransferase antigen complex from Streptococcus mutants strain 6715 were investigated in hamsters. All enzyme-linked immunosorbent assay was used to measure the antibody quantity and isotype, and a [14C]glucosyl-labeled sucrose incorporation assay w...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Smith DJ,Taubman MA,Ebersole JL

    更新日期:1980-05-01 00:00:00

  • Antimycobacterial calixarenes enhance innate defense mechanisms in murine macrophages and induce control of Mycobacterium tuberculosis infection in mice.

    abstract::Tuberculosis remains the leading cause of death among infectious diseases, accounting for more than two million deaths annually. The incidence of the disease is increasing globally, partially because of the resurgence of drug-resistant strains of Mycobacterium tuberculosis. Calixarenes are macrocyclic oligomers, some ...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Colston MJ,Hailes HC,Stavropoulos E,Hervé AC,Hervé G,Goodworth KJ,Hill AM,Jenner P,Hart PD,Tascon RE

    更新日期:2004-11-01 00:00:00

  • Induction of inflammatory cytokines in bovine alveolar macrophages following stimulation with Pasteurella haemolytica lipopolysaccharide.

    abstract::Bovine tumor necrosis factor alpha (TNF-alpha) and interleukin-1 beta (IL-1 beta) cDNAs were generated by reverse transcription and then by PCR amplification from lipopolysaccharide (LPS)-stimulated alveolar macrophage RNA. The amplified cDNAs were cloned into pPow and expressed in Escherichia coli DH5 alpha. The expr...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Yoo HS,Maheswaran SK,Lin G,Townsend EL,Ames TR

    更新日期:1995-02-01 00:00:00

  • Isolation, structure, and immunogenicity of Pseudomonas aeruginosa immunotype 4 high-molecular-weight polysaccharide.

    abstract::A high-molecular-weight, immunogenic form of the lipopolysaccharide O side chain of Pseudomonas aeruginosa Fisher immunotype 4 (type 4, International Antigenic Typing System 1, Lanyi O:6) was isolated and characterized. Analysis by nuclear magnetic resonance spectroscopy confirmed the structural similarity of this hig...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Pier GB,Pollack M

    更新日期:1989-02-01 00:00:00

  • Shigella flexneri O-antigen epitopes: chemical and immunochemical analyses reveal that epitopes of type III and group 6 antigens are identical.

    abstract::In the present investigation we studied the nature of a Shigella flexneri O antigen, the type III antigen. No structural entity has yet been attributed to this antigen. Phenol-water-extracted lipopolysaccharides (LPSs) from all S. flexneri serotypes possessing either group 6 antigen or both type III antigen and group ...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Carlin NI,Wehler T,Lindberg AA

    更新日期:1986-07-01 00:00:00

  • Mechanism of pertussis toxin B oligomer-mediated protection against Bordetella pertussis respiratory infection.

    abstract::Immunization with the B oligomer of pertussis toxin protected neonatal mice from a lethal respiratory challenge with Bordetella pertussis. All mice immunized with 8 micrograms of B oligomer survived aerosol challenge and had peripheral leukocyte counts and weight gains similar to those of mice immunized with pertussis...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Shahin RD,Witvliet MH,Manclark CR

    更新日期:1990-12-01 00:00:00

  • Pasteurella haemolytica leukotoxin enhances production of leukotriene B4 and 5-hydroxyeicosatetraenoic acid by bovine polymorphonuclear leukocytes.

    abstract::The influence of the leukotoxin of Pasteurella haemolytica on the generation of arachidonic acid metabolites by bovine polymorphonuclear leukocytes (PMNs) was investigated. PMNs released 5-, 12-, and 15-hydroxyeicosatetraenoic acids (5-, 12-, and 15-HETE) and leukotriene B4 (LTB4) upon stimulation with arachidonic aci...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Henricks PA,Binkhorst GJ,Drijver AA,Nijkamp FP

    更新日期:1992-08-01 00:00:00

  • Leptospira santorosai Serovar Shermani detergent extract induces an increase in fibronectin production through a Toll-like receptor 2-mediated pathway.

    abstract::Leptospirosis can activate inflammatory responses through Toll-like receptors (TLRs) and may cause renal tubulointerstitial fibrosis characterized by the accumulation of extracellular matrix (ECM). We have previously demonstrated that Leptospira santorosai serovar Shermani detergent extract stimulates ECM accumulation...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Tian YC,Hung CC,Li YJ,Chen YC,Chang MY,Yen TH,Hsu HH,Wu MS,Phillips A,Yang CW

    更新日期:2011-03-01 00:00:00

  • Host inflammatory response inhibits Escherichia coli O157:H7 adhesion to gut epithelium through augmentation of mucin expression.

    abstract::Escherichia coli O157:H7, a major Shiga toxin-producing pathogen, has a low infectious dose and causes serious illness in humans. The gastrointestinal tract of cattle is the primary reservoir of E. coli O157:H7, and thus, it is critical to eliminate or reduce E. coli O157:H7 gut colonization. Given that E. coli O157:H...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Xue Y,Zhang H,Wang H,Hu J,Du M,Zhu MJ

    更新日期:2014-05-01 00:00:00

  • Impaired control of Brucella melitensis infection in Rag1-deficient mice.

    abstract::After intranasal inoculation, Brucella melitensis chronically infects the mononuclear phagocyte system in BALB/c mice, but it causes no apparent illness. Adaptive immunity, which can be transferred by either T cells or antibody from immune to naive animals, confers resistance to challenge infection. The role of innate...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Izadjoo MJ,Polotsky Y,Mense MG,Bhattacharjee AK,Paranavitana CM,Hadfield TL,Hoover DL

    更新日期:2000-09-01 00:00:00

  • Contribution of clumping factor B to pathogenesis of experimental endocarditis due to Staphylococcus aureus.

    abstract::Staphylococcus aureus Newman with an insertion mutation in clfB, the gene encoding clumping factor B, only marginally decreased infection rate (P>0.05) in rats with experimental endocarditis. In contrast, clfB complementation on a multicopy plasmid significantly increased infectivity (P<0.05) over the deleted mutants....

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Entenza JM,Foster TJ,Ni Eidhin D,Vaudaux P,Francioli P,Moreillon P

    更新日期:2000-09-01 00:00:00

  • Effect of Trichinella spiralis infection on passive cutaneous anaphylaxis in mice.

    abstract::Infection of CFW mice with Trichinella spiralis induced a state of relative unresponsiveness to passive cutaneous anaphylaxis (PCA) induced with hen egg albumin and its corresponding antibodies. The unresponsiveness was to PCA produced either with immunoglobulin G1 (IgG1) or IgE type of antibodies, but was more pronou...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Munoz JJ,Cole RL

    更新日期:1977-01-01 00:00:00

  • Pyruvate formate lyase is required for pneumococcal fermentative metabolism and virulence.

    abstract::Knowledge of the in vivo physiology and metabolism of Streptococcus pneumoniae is limited, even though pneumococci rely on efficient acquisition and metabolism of the host nutrients for growth and survival. Because the nutrient-limited, hypoxic host tissues favor mixed-acid fermentation, we studied the role of the pne...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Yesilkaya H,Spissu F,Carvalho SM,Terra VS,Homer KA,Benisty R,Porat N,Neves AR,Andrew PW

    更新日期:2009-12-01 00:00:00

  • Failure to detect cell-mediated cytotoxicity against Chlamydia trachomatis-infected cells.

    abstract::Mice were infected with Chlamydia trachomatis (L2/434) and at various intervals after infection their lymphoid cells were tested directly for cytotoxic activity against Chlamydia-infected L929 cells. In a modified cell-mediated lympholysis assay, splenic, lymph node, and peritoneal exudate cells did not significantly ...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Pavia CS,Schachter J

    更新日期:1983-03-01 00:00:00

  • GapA and CrmA coexpression is essential for Mycoplasma gallisepticum cytadherence and virulence.

    abstract::It was previously demonstrated that avirulent Mycoplasma gallisepticum strain R(high) (passage 164) is lacking three proteins that are expressed in its virulent progenitor, strain R(low) (passage 15). These proteins were identified as the cytadhesin molecule GapA, the putative cytadhesin-related molecule CrmA, and a c...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Papazisi L,Frasca S Jr,Gladd M,Liao X,Yogev D,Geary SJ

    更新日期:2002-12-01 00:00:00

  • Population structure of the Bacillus cereus group as determined by sequence analysis of six housekeeping genes and the plcR Gene.

    abstract::The population structure of the Bacillus cereus group (52 strains of B. anthracis, B. cereus, and B. thuringiensis) was investigated by sequencing seven gene fragments (rpoB, gyrB, pycA, mdh, mbl, mutS, and plcR). Most of the strains were classifiable into two large subgroups in six housekeeping gene trees but not in ...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Ko KS,Kim JW,Kim JM,Kim W,Chung SI,Kim IJ,Kook YH

    更新日期:2004-09-01 00:00:00