Hepatic cholesterol metabolism in patients with cholesterol gallstones: enhanced intracellular transport of cholesterol.


BACKGROUND & AIMS:Alterations of hepatic cholesterol metabolism in patients with cholesterol gallstones are still controversial. This study investigated whether hepatic cholesterol metabolism is altered in Japanese patients with cholesterol gallstones. METHODS:In this systematic study of 24 middle-aged nonobese and nondiabetic Japanese patients who had cholesterol gallstones and were undergoing elective cholecystectomy, an analysis of three regulatory enzymes in the cholesterol metabolism, as well as cytosolic total and free cholesterol levels and sterol carrier protein 2/nonspecific lipid transfer protein (SCP2/nsLTP) levels, was conducted using liver biopsy samples obtained during surgery. RESULTS:The activities of 3-hydroxy-3-methyl-glutaryl-coenzyme A (HMG-CoA) reductase, cholesterol 7 alpha-hydroxylase, and acyl-CoA/cholesterol acyltransferase were not significantly different between patients and controls. Nevertheless, patients with gallstones showed tendencies for elevated HMG-CoA reductase activity and protein content and decreased cholesterol 7 alpha-hydroxylase activities. As anticipated, serum levels of 7 alpha-hydroxycholesterol and squalene paralleled these findings. The patients with gallstones also had significantly increased cytosolic total and free cholesterol levels (P < 0.001), which correlated strongly with increased cytosolic levels of SCP2/nsLTP (r = 0.80, P < 0.001 and r = 0.81, P < 0.001, respectively). CONCLUSIONS:The results suggest that intracellular cholesterol transport is enhanced in patients with cholesterol gallstones.






Ito T,Kawata S,Imai Y,Kakimoto H,Trzaskos JM,Matsuzawa Y




Has Abstract


1996-05-01 00:00:00














  • Seasonal variation in flares of inflammatory bowel disease.

    abstract:BACKGROUND & AIMS:Previous research has yielded conflicting data as to whether the natural history of inflammatory bowel disease follows a seasonal pattern. The purpose of this study was to determine whether relapse of inflammatory bowel disease follows a seasonal pattern either across a cohort of patients or within in...


    pub_type: 杂志文章


    authors: Lewis JD,Aberra FN,Lichtenstein GR,Bilker WB,Brensinger C,Strom BL

    更新日期:2004-03-01 00:00:00

  • Glycine-extended gastrin synergizes with gastrin 17 to stimulate acid secretion in gastrin-deficient mice.

    abstract:BACKGROUND & AIMS:Studies in gastrin-deficient mice have demonstrated critical roles for gastrin peptides in the regulation of gastric acid secretion, but the relative contributions of amidated (G-17) and glycine-extended (G17-Gly) gastrin remain unclear. We examined the effects of these 2 forms of gastrin on acid secr...


    pub_type: 杂志文章


    authors: Chen D,Zhao CM,Dockray GJ,Varro A,Van Hoek A,Sinclair NF,Wang TC,Koh TJ

    更新日期:2000-09-01 00:00:00

  • In vitro characterization of human intestinal intraepithelial lymphocytes.

    abstract::Enriched populations of human intestinal and colonic intraepithelial lymphocytes were isolated separate from lamina propria lymphocytes. These populations, and peripheral blood mononuclear cells, were characterized for membrane features, lymphocyte subsets, and proliferative potential to the nonspecific mitogenic lect...


    pub_type: 杂志文章


    authors: Greenwood JH,Austin LL,Dobbins WO 3rd

    更新日期:1983-11-01 00:00:00

  • Synergistic inhibitory effects of gastrin and histamine receptor antagonists on Helicobacter-induced gastric cancer.

    abstract:BACKGROUND & AIMS:Apart from its importance as an acid secretogogue, the role of histamine as a downstream target of gastrin has not been fully explored. Previous studies have shown that the combination of hypergastrinemia and Helicobacter infection resulted in accelerated gastric cancer in mice. We used this model to ...


    pub_type: 杂志文章


    authors: Takaishi S,Cui G,Frederick DM,Carlson JE,Houghton J,Varro A,Dockray GJ,Ge Z,Whary MT,Rogers AB,Fox JG,Wang TC

    更新日期:2005-06-01 00:00:00

  • Decrease of a hepatic binding protein specific for asialoglycoproteins with accumulation of serum asialoglycoproteins in galactosamine-treated rats.

    abstract::Acute liver injury was induced experimentally in rats by a single injection of a large amount of D-galactosamine. Hepatocellular damage was apparent from decrease of total serum proteins, marked release of transaminases into the circulation, precipitous decrease of total microsomal proteins, and intracellular enzymes ...


    pub_type: 杂志文章


    authors: Sawamura T,Kawasato S,Shiozaki Y,Sameshima Y,Nakada H,Tashiro Y

    更新日期:1981-09-01 00:00:00

  • Effects of omeprazole on intragastric pH and plasma gastrin are dependent on the CYP2C19 polymorphism.

    abstract:BACKGROUND & AIMS:Omeprazole is metabolized by cytochrome P450 (CYP2C19). The activity of this enzyme is polymorphic, with incidences of poor metabolizers (PMs), heterozygous extensive metabolizers (EMs), and homozygous EMs in white populations of 3%, 30%, and 67%, respectively. The importance of the CYP2C19 polymorphi...


    pub_type: 杂志文章


    authors: Sagar M,Tybring G,Dahl ML,Bertilsson L,Seensalu R

    更新日期:2000-09-01 00:00:00

  • Once-daily, high-concentration MMX mesalamine in active ulcerative colitis.

    abstract:BACKGROUND & AIMS:SPD476 (LIALDA in the US; MEZAVANT in the EU; otherwise known as MMX mesalamine; Shire Pharmaceuticals Inc., Wayne, PA, under license from Giuliani SpA, Milan, Italy) is a novel, once-daily, high-strength (1.2 g/tablet) formulation of mesalamine, utilizing MMX Multi Matrix System (MMX) technology desi...


    pub_type: 杂志文章,多中心研究,随机对照试验


    authors: Kamm MA,Sandborn WJ,Gassull M,Schreiber S,Jackowski L,Butler T,Lyne A,Stephenson D,Palmen M,Joseph RE

    更新日期:2007-01-01 00:00:00

  • Erythropoietic protoporphyria: unusual skin and neurological problems after liver transplantation.

    abstract::The case of a woman with protoporphyria who developed liver failure and underwent liver transplantation is described. During the pretransplant episode of liver failure she developed quadriparesis that rapidly progressed after transplantation to a severe polyneuropathy. Following transplantation she also developed a se...


    pub_type: 杂志文章


    authors: Herbert A,Corbin D,Williams A,Thompson D,Buckels J,Elias E

    更新日期:1991-06-01 00:00:00

  • Olsalazine sodium in the treatment of ulcerative colitis among patients intolerant of sulfasalazine. A prospective, randomized, placebo-controlled, double-blind, dose-ranging clinical trial.

    abstract::Sixty-six outpatients with active ulcerative colitis who were intolerant of sulfasalazine were treated in a double-blind randomized trial. They received placebo or olsalazine sodium in daily doses of 0.75, 1.5, or 3 g. Overall, 35% of patients receiving olsalazine improved clinically, compared to 16% of patients recei...


    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Meyers S,Sachar DB,Present DH,Janowitz HD

    更新日期:1987-12-01 00:00:00

  • Human intestinal mononuclear cells. II. Demonstration of a naturally occurring subclass of T cells which respond in the allogeneic mixed leukocyte reaction but do not effect cell-mediated lympholysis.

    abstract::We have examined the T-cell functional capabilities of human intestinal mononuclear cells isolated from surgically obtained normal and inflammatory bowel disease intestinal specimens. Intestinal mononuclear cells have T cells present which respond to the mitogenic lectins E-PHA, Con A, and PWM and to foreign cell-surf...


    pub_type: 杂志文章


    authors: MacDermott RP,Bragdon MJ,Jenkins KM,Franklin GO,Shedlofsky S,Kodner IJ

    更新日期:1981-04-01 00:00:00

  • Chronic ethanol consumption impairs cellular immune responses against HCV NS5 protein due to dendritic cell dysfunction.

    abstract:BACKGROUND & AIMS:Alcoholic patients with and without chronic liver disease have a high incidence of infection with hepatitis C virus (HCV). Long-term ethanol consumption in mice has been associated with a strikingly reduced CD8(+) cytotoxic T-lymphocyte (CTL) response to HCV nonstructural proteins following DNA-based ...


    pub_type: 杂志文章


    authors: Aloman C,Gehring S,Wintermeyer P,Kuzushita N,Wands JR

    更新日期:2007-02-01 00:00:00

  • A proinflammatory, antiapoptotic phenotype underlies the susceptibility to acute pancreatitis in cystic fibrosis transmembrane regulator (-/-) mice.

    abstract:BACKGROUND & AIMS:Cystic fibrosis transmembrane regulator (CFTR) gene mutations are associated with pancreatic insufficiency and pancreatitis. Chronic pancreatitis, including cystic fibrosis-related disease, may exist as a continuum between acute and chronic disease and may manifest as recurrent pain. We hypothesized t...


    pub_type: 杂志文章


    authors: Dimagno MJ,Lee SH,Hao Y,Zhou SY,McKenna BJ,Owyang C

    更新日期:2005-08-01 00:00:00

  • Effect of glucagon infusion on the renal clearance of amylase relative to creatinine.

    abstract::Recent data seem to support a tubular defect as the mechanism of the elevated renal clearance of amylase relative to creatinine in acute pancreatitis. Glucagon has been proposed by some to be an important factor in this phenomenon. To examine the role of glucagon as this "tubular dysfunction factor", we investigated t...


    pub_type: 临床试验,杂志文章


    authors: Tedesco FJ,Davila E,Gardner LB

    更新日期:1978-10-01 00:00:00

  • Thromboxane A2 synthase inhibitors prevent production of infectious hepatitis C virus in mice with humanized livers.

    abstract:BACKGROUND & AIMS:A 3-dimensional (3D) culture system for immortalized human hepatocytes (HuS-E/2 cells) recently was shown to support the lifecycle of blood-borne hepatitis C virus (HCV). We used this system to identify proteins that are active during the HCV lifecycle under 3D culture conditions. METHODS:We compared...


    pub_type: 杂志文章


    authors: Abe Y,Aly HH,Hiraga N,Imamura M,Wakita T,Shimotohno K,Chayama K,Hijikata M

    更新日期:2013-09-01 00:00:00

  • Influence of cigarette smoking on healing and relapse in duodenal ulcer disease.

    abstract::There are conflicting reports on the influence of cigarette smoking on healing in patients with duodenal ulcer; some studies show an adverse effect on healing rate and others no effect. This study reports the influence of smoking on short-term healing and relapse rate in 135 patients with duodenal ulcer who were treat...


    pub_type: 临床试验,杂志文章


    authors: Korman MG,Hansky J,Eaves ER,Schmidt GT

    更新日期:1983-10-01 00:00:00

  • Peginterferon alfa-2a (40 kilodaltons) and ribavirin in patients with chronic hepatitis C and normal aminotransferase levels.

    abstract:BACKGROUND & AIMS:Patients with chronic hepatitis C and persistently normal alanine aminotransferase (ALT) levels have been routinely excluded from large randomized treatment trials; consequently, the efficacy and safety of antiviral therapy in this population are unknown. METHODS:Patients with at least 3 normal ALT v...


    pub_type: 临床试验,杂志文章,多中心研究,随机对照试验


    authors: Zeuzem S,Diago M,Gane E,Reddy KR,Pockros P,Prati D,Shiffman M,Farci P,Gitlin N,O'Brien CB,Lamour F,Lardelli P,PEGASYS Study NR16071 Investigator Group.

    更新日期:2004-12-01 00:00:00

  • Hepatocellular Carcinomas With Mutational Activation of Beta-Catenin Require Choline and Can Be Detected by Positron Emission Tomography.

    abstract:BACKGROUND & AIMS:In one-third of hepatocellular carcinomas (HCCs), cancer cells have mutations that activate β-catenin pathway. These cells have alterations in glutamine, bile, and lipid metabolism. We investigated whether positron emission tomography (PET) imaging allows identification of altered metabolic pathways t...


    pub_type: 杂志文章


    authors: Gougelet A,Sartor C,Senni N,Calderaro J,Fartoux L,Lequoy M,Wendum D,Talbot JN,Prignon A,Chalaye J,Imbeaud S,Zucman-Rossi J,Tordjmann T,Godard C,Bossard P,Rosmorduc O,Amaddeo G,Colnot S

    更新日期:2019-09-01 00:00:00

  • Neural stem cell transplantation in the stomach rescues gastric function in neuronal nitric oxide synthase-deficient mice.

    abstract:BACKGROUND & AIMS:Nitric oxide is a major inhibitory neurotransmitter in the enteric nervous system. Loss or dysfunction of nitrinergic neurons is associated with serious disruptions of motility, intractable symptoms, and long-term suffering. The aim of this study was to evaluate the effect of intrapyloric transplantat...


    pub_type: 评论,杂志文章


    authors: Micci MA,Kahrig KM,Simmons RS,Sarna SK,Espejo-Navarro MR,Pasricha PJ

    更新日期:2005-12-01 00:00:00

  • CD4+NKG2D+ T cells in Crohn's disease mediate inflammatory and cytotoxic responses through MICA interactions.

    abstract:BACKGROUND & AIMS:Crohn's disease (CD) is an inflammatory bowel disease characterized by uncontrolled immune responses to bacterial flora, with excessive activation of T lymphocytes. MICA is a stress-induced major histocompatibility complex-related molecule expressed on normal intestinal epithelial cells (IECs) and rec...


    pub_type: 杂志文章


    authors: Allez M,Tieng V,Nakazawa A,Treton X,Pacault V,Dulphy N,Caillat-Zucman S,Paul P,Gornet JM,Douay C,Ravet S,Tamouza R,Charron D,Lémann M,Mayer L,Toubert A

    更新日期:2007-06-01 00:00:00

  • alpha-1-antitrypsin deficiency in liver disease: the extent of the problem.

    abstract::A prospective screening program was undertaken at the Royal Free Hospital, London, to ascertain the incidence of alpha-1-antitrypsin (AAT) deficiency in patients with liver disease. Quantitative determinations of serum alpha-1-antitrypsin were performed on 469 patients with hepatobiliary disease and 98 subjects with n...


    pub_type: 杂志文章


    authors: Fisher RL,Taylor L,Sherlock S

    更新日期:1976-10-01 00:00:00

  • Trypsin reduces pancreatic ductal bicarbonate secretion by inhibiting CFTR Cl⁻ channels and luminal anion exchangers.

    abstract:BACKGROUND & AIMS:The effects of trypsin on pancreatic ductal epithelial cells (PDECs) vary among species and depend on the localization of proteinase-activated receptor 2 (PAR-2). We compared PAR-2 localization in human and guinea-pig PDECs, and used isolated guinea pig ducts to study the effects of trypsin and a PAR-...


    pub_type: 杂志文章


    authors: Pallagi P,Venglovecz V,Rakonczay Z Jr,Borka K,Korompay A,Ozsvári B,Judák L,Sahin-Tóth M,Geisz A,Schnúr A,Maléth J,Takács T,Gray MA,Argent BE,Mayerle J,Lerch MM,Wittmann T,Hegyi P

    更新日期:2011-12-01 00:00:00

  • Enteropathogenic Escherichia coli infection inhibits intestinal serotonin transporter function and expression.

    abstract:BACKGROUND & AIMS:Serotonin transporter (SERT) plays a critical role in regulating serotonin (5-hydroxytryptamine [5-HT]) availability in the gut. Elevated 5-HT levels are associated with diarrheal conditions such as irritable bowel syndrome and enteric infections. Whether alteration in SERT activity contributes to the...


    pub_type: 杂志文章


    authors: Esmaili A,Nazir SF,Borthakur A,Yu D,Turner JR,Saksena S,Singla A,Hecht GA,Alrefai WA,Gill RK

    更新日期:2009-12-01 00:00:00

  • Tumor Necrosis Factor-producing T-regulatory Cells Are Associated With Severe Liver Injury in Patients With Acute Hepatitis A.

    abstract:BACKGROUND AND AIMS:CD4+CD25+Foxp3+ T-regulatory (Treg) cells control immune responses and maintain immune homeostasis. However, under inflammatory conditions, Treg cells produce cytokines that promote inflammation. We investigated production of tumor necrosis factor (TNF) by Treg cells in patients with acute hepatitis...


    pub_type: 杂志文章


    authors: Choi YS,Jung MK,Lee J,Choi SJ,Choi SH,Lee HW,Lee JJ,Kim HJ,Ahn SH,Lee DH,Kim W,Park SH,Huh JR,Kim HP,Park JY,Shin EC

    更新日期:2018-03-01 00:00:00

  • Oral cholic acid for hereditary defects of primary bile acid synthesis: a safe and effective long-term therapy.

    abstract:BACKGROUND & AIMS:Oral bile acid replacement has been shown to be an effective therapy in primary bile acid synthesis defects, but to date there have been no reports of the long-term effects of this therapy. The aim of the study was to evaluate the long-term effectiveness and safety of cholic acid (CA) therapy. METHOD...


    pub_type: 临床试验,杂志文章


    authors: Gonzales E,Gerhardt MF,Fabre M,Setchell KD,Davit-Spraul A,Vincent I,Heubi JE,Bernard O,Jacquemin E

    更新日期:2009-10-01 00:00:00

  • Negative feedback regulation of the ileal bile acid transport system in rodents.

    abstract:BACKGROUND:Active transport of conjugated bile acids by ileal enterocytes is a key mechanism for conservation of the bile acid pool. Experiments were performed to determine whether such transport is regulated by substrate load. METHODS:Using anesthetized biliary fistula guinea pigs or rats, the ileum was perfused with...


    pub_type: 杂志文章


    authors: Lillienau J,Crombie DL,Munoz J,Longmire-Cook SJ,Hagey LR,Hofmann AF

    更新日期:1993-01-01 00:00:00

  • The pregnane X receptor locus is associated with susceptibility to inflammatory bowel disease.

    abstract:BACKGROUND & AIMS:The pregnane X receptor (PXR) regulates an array of genes involved in the response to xenobiotics. Evidence from several studies suggests that xenobiotic metabolism may play a role in inflammatory bowel disease (IBD) and that low levels of PXR may be associated with disease expression. The aim of this...


    pub_type: 杂志文章


    authors: Dring MM,Goulding CA,Trimble VI,Keegan D,Ryan AW,Brophy KM,Smyth CM,Keeling PW,O'Donoghue D,O'Sullivan M,O'Morain C,Mahmud N,Wikström AC,Kelleher D,McManus R

    更新日期:2006-02-01 00:00:00

  • Impaired lithocholate sulfation in the rhesus monkey: a possible mechanism for chenodeoxycholate toxicity.

    abstract::Radioactive lithocholate was administered intravenously to female rhesus monkeys with an exteriorized enterohepatic circulation, and the chemical form of biliary radioactivity was defined by thin layer chromatography. Most radioactivity was excreted in bile in the form of unsulfated conjugates (77%), indicating impair...


    pub_type: 杂志文章


    authors: Gadacz TR,Allan RN,Mack E,Hofmann AF

    更新日期:1976-06-01 00:00:00

  • Growth rate of asymptomatic hepatocellular carcinoma and its clinical implications.

    abstract::The growth rate of 31 asymptomatic hepatocellular carcinomas (diameter less than or equal to 5 cm) discovered in 28 patients by a prospective screening program was determined by real-time ultrasonography over 36-860 days. Except for one tumor that shrank on follow-up, the doubling time ranged from 29 to 398 days, with...


    pub_type: 杂志文章


    authors: Sheu JC,Sung JL,Chen DS,Yang PM,Lai MY,Lee CS,Hsu HC,Chuang CN,Yang PC,Wang TH

    更新日期:1985-08-01 00:00:00

  • Ultrasound-Mediated Delivery of RNA to Colonic Mucosa of Live Mice.

    abstract:BACKGROUND & AIMS:It is a challenge to deliver nucleic acids to gastrointestinal (GI) tissues due to their size and need for intracellular delivery. They are also extremely susceptible to degradation by nucleases, which are ubiquitous in the GI tract. We investigated whether ultrasound, which can permeabilize tissue th...


    pub_type: 杂志文章


    authors: Schoellhammer CM,Lauwers GY,Goettel JA,Oberli MA,Cleveland C,Park JY,Minahan D,Chen Y,Anderson DG,Jaklenec A,Snapper SB,Langer R,Traverso G

    更新日期:2017-04-01 00:00:00

  • Susceptibility to severe ulcerative colitis is associated with polymorphism in the central MHC gene IKBL.

    abstract:BACKGROUND & AIMS:IKBL gene lies telomeric of the tumor necrosis factor cluster in the central major histocompatibility complex and carries a structural polymorphism at position +738. In the Spanish white population, we found the IKBL+738(C) allele in haplotypes carrying either HLA-DRB1(*)1501 or -DRB1(*)0103. Because ...


    pub_type: 杂志文章


    authors: de la Concha EG,Fernandez-Arquero M,Lopez-Nava G,Martin E,Allcock RJ,Conejero L,Paredes JG,Diaz-Rubio M

    更新日期:2000-12-01 00:00:00