Transferrin receptors of Neisseria meningitidis: promising candidates for a broadly cross-protective vaccine.


:Production of a meningococcal vaccine capable of generating long-lasting immunity in all age groups is still a high priority worldwide. Iron-regulated outer-membrane proteins have attracted considerable attention in recent years and it has become increasingly evident that the meningococcal transferrin-binding proteins, TBP1 and TBP2, have characteristics compatible with a safe and broadly cross-reactive vaccine candidate. Both TBPs are surface-exposed and immunogenic in man and animals, and antibodies to their native structure are bactericidal to homologous and many heterologous strains. These include strains from various serogroups, serotypes and serosubtypes, with no obvious correlation between bactericidal activity and the identity of the strains or the molecular mass of the heterogeneous TBP2 molecule. A meningococcal vaccine based on, or enriched with, undenatured TBPs from one or more strains, in combination with conventional polysaccharide-based vaccines, might increase the spectrum of strains against which protection can be achieved to include serogroup B strains. In this review, the structure-function and immunological properties of TBP1 and TBP2 are discussed.


J Med Microbiol


Ala'Aldeen DA




Has Abstract


1996-04-01 00:00:00












  • Distribution and genetic location of Tn7 in trimethoprim-resistant Escherichia coli.

    abstract::A series of 178 strains of Escherichia coli, highly resistant to trimethoprim, isolated from hospital patients and patients in the community between 1979 and 1983, was examined for the presence of Tn7 on a plasmid or on the chromosome only, by transposition to RP4 and restriction endonuclease digestion with Hind III. ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Kraft CA,Timbury MC,Platt DJ

    更新日期:1986-09-01 00:00:00

  • Recovery of interfering and beta-lactamase-producing bacteria from group A beta-haemolytic streptococci carriers and non-carriers.

    abstract::The purpose of this study was to compare the frequency of recovery of aerobic and anaerobic organisms with interfering capability against group A beta-haemolytic streptococci (GABHS) and beta-lactamase-producing bacteria (BLPB) from the tonsils of GABHS carriers and non-carriers. The presence of aerobic and anaerobic ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Brook I,Gober AE

    更新日期:2006-12-01 00:00:00

  • Characterization of the charge variants of L2 beta-lactamase in Stenotrophomonas maltophilia.

    abstract::Stenotrophomonas maltophilia KH has two acid beta-lactamases with isoelectric points (pIs) of 4.6 and 5.4, and several basic beta-lactamases (pIs >7.0) that produce a ladder-shaped pattern by IEF. An isogenic L2 mutant, KHL2(xylE), was constructed by gene replacement. From IEF and native PAGE zymograms of strains KH a...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Hu RM,Chiang KH,Chang YC,Yang TC

    更新日期:2009-03-01 00:00:00

  • The microbial flora of the rectal mucosa and faeces of patients with Crohn's disease before and during antimicrobial chemotherapy.

    abstract::The faecal flora and mucosa-associated flora (MAF) of rectal biopsy material from 12 patients with active Crohn's disease were studied before and during treatment with a combination of metronidazole and cotrimoxazole given orally for at least 2 weeks. The total faecal flora was greater than the MAF although the propor...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Hudson MJ,Hill MJ,Elliott PR,Berghouse LM,Burnham WR,Lennard-Jones JE

    更新日期:1984-12-01 00:00:00

  • High prevalence of multidrug-resistant Escherichia coli isolates from children with and without diarrhoea and their susceptibility to the antibacterial activity of extracts/fractions of fruits native to Mexico.

    abstract:PURPOSE:This paper aims to evaluate the antimicrobial resistance of Esherichia coli isolates from children under 5 years old, with and without diarrhoea, who were hospital outpatients in Culiacan, Sinaloa, Mexico. It also looks at the antimicrobial activity of fruit extracts against selected multidrug-resistant (MDR) E...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Uribe-Beltrán MJ,Ahumada-Santos YP,Díaz-Camacho SP,Eslava-Campos CA,Reyes-Valenzuela JE,Báez-Flores ME,Osuna-Ramírez I,Delgado-Vargas F

    更新日期:2017-07-01 00:00:00

  • Lesions produced by Clostridium butyricum strain CB 1002 in ligated intestinal loops in guinea pigs.

    abstract::Heated spores (80 degrees C, 10 min) of Clostridium butyricum strain CB 1002 isolated from a fatal case of necrotising enterocolitis in a human neonate were inoculated into ligated intestinal loops prepared in young conventional guinea pigs. Necropsy findings 18 h later included congestion, patchy haemorrhage of the i...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Popoff MR,Ravisse P

    更新日期:1985-06-01 00:00:00

  • Detection of Mycobacterium tuberculosis in latently infected lungs by immunohistochemistry and confocal microscopy.

    abstract::Detection of latent Mycobacterium tuberculosis is a challenge in the diagnosis of asymptomatic, subclinical tuberculosis. We report the development of an immunofluorescence technique to visualize and enumerate M. tuberculosis in latently infected rabbit lungs where no acid-fast-stained organisms were seen and no culti...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Subbian S,Eugenin E,Kaplan G

    更新日期:2014-11-01 00:00:00

  • The resolution of bacteroides lipopolysaccharides by polyacrylamide gel electrophoresis.

    abstract::The lipopolysaccharides (LPS) of the 10 species of the genus Bacteroides (sensu stricto) were extracted by the proteinase K method and their resolution compared by several methods of polyacrylamide gel electrophoresis (PAGE). These included sodium dodecyl sulphate (SDS)-PAGE with and without urea, polyacrylamide gradi...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Maskell JP

    更新日期:1991-05-01 00:00:00

  • The rectal mucosa-associated microflora in patients with ulcerative colitis.

    abstract::The rectal mucosa-associated flora (MAF) of patients with ulcerative colitis has been studied in 25 patients with newly diagnosed disease, 20 with relapse of existing disease, and 44 who were in remission. Patients with active disease were re-examined twice during treatment. The MAF was simpler and less dense than the...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Hartley MG,Hudson MJ,Swarbrick ET,Hill MJ,Gent AE,Hellier MD,Grace RH

    更新日期:1992-02-01 00:00:00

  • Co-elimination of mec and spa genes in Staphylococcus aureus and the effect of agr and protein A production on bacterial adherence to cell monolayers.

    abstract::Phenotypic loss of protein A production was tested in six methicillin-resistant (McR) Staphylococcus aureus (MRSA) isolates and their isogenic methicillin-sensitive (McS) variants by a radiolabelled IgG-binding assay with washed cells and by Western blotting of supernates prepared from lysed washed cells. Genomic DNA ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Poston SM,Glancey GR,Wyatt JE,Hogan T,Foster TJ

    更新日期:1993-12-01 00:00:00

  • In vitro ceftaroline combinations against meticillin-resistant Staphylococcus aureus.

    abstract::We studied in vitro ceftaroline combinations against 61 meticillin-resistant Staphylococcus aureus isolates; 18 of them were also resistant to linezolid, using overlapping E-test method. Daptomycin-ceftaroline combination obtained lower fractional inhibitory concentration values, in comparison with those including van...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: García AB,Candel FJ,López L,Chiarella F,Viñuela-Prieto JM

    更新日期:2016-10-01 00:00:00

  • The identification of Gardnerella vaginalis.

    abstract::A collection of 72 strains of catalase-negative gram-positive, -negative and -variable cocco-bacilli isolated from samples of vaginal discharge from women with non-specific vaginal infection was examined in an attempt to develop an identification system for Gardnerella vaginalis that could be used in a diagnostic labo...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Taylor E,Phillips I

    更新日期:1983-02-01 00:00:00

  • Inhibition of quorum-sensing-controlled virulence factors of Pseudomonas aeruginosa by Murraya koenigii essential oil: a study in a Caenorhabditis elegans infectious model.

    abstract::The global emergence of antibiotic-resistant strains of Pseudomonas aeruginosa poses a major threat in both hospital environments and the community. P. aeruginosa is an opportunistic human pathogen, and it also infects a wide range of model organisms including the nematode Caenorhabditis elegans. Quorum sensing (QS) m...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Ganesh PS,Rai RV

    更新日期:2016-12-01 00:00:00

  • Protection against experimental salmonellosis in mice and sheep by immunisation with aromatic-dependent Salmonella typhimurium.

    abstract::Mice immunised by the oral or intraperitoneal route with a live aromatic-dependent strain of Salmonella typhimurium exhibited significantly less protection against oral challenge with 50 LD50 of an ovine isolate of S. typhimurium (12313) than when a bovine isolate with the same O antigens and phage-type as strain 1231...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Mukkur TK,McDowell GH,Stocker BA,Lascelles AK

    更新日期:1987-08-01 00:00:00

  • An outbreak of Shigella boydii serotype 20 in January 2015 amongst United Kingdom healthcare workers involved in the Ebola response in Sierra Leone.

    abstract::In January 2015, Public Health England and the United Kingdom (UK) Ministry of Defence investigated cases of diarrhoea and fever in military personnel recently returned to the UK after supporting the response to the Ebola epidemic in Sierra Leone. Tests for Ebola virus infection were negative. PCR tests detected the i...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Todkill D,Pudney R,Terrell A,Tuck J,Hutley E,Dallman TJ,Jenkins C,Manuel R,Wade M,Puleston R,Hawker J

    更新日期:2018-11-01 00:00:00

  • Passive immunisation against experimental salmonellosis in mice by orally administered hen egg-yolk antibodies specific for 14-kDa fimbriae of Salmonella enteritidis.

    abstract::Chickens were immunised with a preparation of purified 14-kDa fimbriae of Salmonella serotype Enteritidis (SEF 14) to raise egg-yolk antibodies for protection trials in mice against subsequent challenge-exposure with the homologous strain of Enteritidis. A pronounced specificity of egg-yolk antibodies against the 14-k...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Peralta RC,Yokoyama H,Ikemori Y,Kuroki M,Kodama Y

    更新日期:1994-07-01 00:00:00

  • Electron microscopy studies on Gardnerella vaginalis grown in conventional and biofilm systems.

    abstract::The cell-wall characteristics of Gardnerella vaginalis grown in conventional and biofilm systems were studied by electron microscopy. The gram-positive nature of the cell wall was confirmed. Novel cell-wall particles which appeared to be associated with cell division were also identified, particularly in organisms of ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Muli FW,Struthers JK,Tarpey PA

    更新日期:1999-02-01 00:00:00

  • Molecular analysis and species diversity of Nocardia in the hospital environment in a developing country, a potential health hazard.

    abstract:PURPOSE:Despite hundreds of reports on the isolation of Nocardia from clinical samples, the presence and diversity of Nocardia species that are capable of survival in a harsh and adverse condition, such as a hospital environment, have not been comprehensively studied. The aim of this study was to assess Nocardia specie...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Rahdar HA,Azadi D,Shojaei H,Daei-Naser A

    更新日期:2017-03-01 00:00:00

  • Occurrence of K1, K5 and O antigens in Escherichia coli isolates from patients with urinary tract infections or bacteraemia.

    abstract::The distribution of K1, K5 and O antigens was examined in 500 clinical strains of Escherichia coli. Of 400 strains from urine, 52% belonged to serogroups O1, O2, O4, O6, O7, O8, O9, O18, O25 and O75; 34% were non-typable (NT) and 14% were autoagglutinable (AA). Antigen K1 was carried by 17% of these strains, and K5 by...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Devine DA,Robinson L,Roberts AP

    更新日期:1989-12-01 00:00:00

  • Effect of Bacteroides fragilis cellular components on chemotactic activity of polymorphonuclear leukocytes towards Escherichia coli.

    abstract::Chemotaxis of polymorphonuclear leukocytes (PMNL) in response to cell components of Bacteroides fragilis alone or in combination with Escherichia coli was evaluated. E. coli produced much more powerful chemotactic factors than B. fragilis. The culture filtrate (CF), outer membrane (OM) preparation, and lipopolysacchar...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Namavar F,Verweij-Van Vught AM,Bal M,MacLaren DM

    更新日期:1987-09-01 00:00:00

  • Hepatitis C virus genotyping in relation to neu-oncoprotein overexpression and the development of hepatocellular carcinoma.

    abstract::The distribution of hepatitis C virus (HCV) genotypes among Egyptian patients positive for anti-HCV was determined and their influence, when combined with neu-oncoprotein overexpression, on the development of hepatocellular carcinoma (HCC) was examined. The study groups included asymptomatic carriers (ASC) and patient...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Zekri AN,Bahnassy AA,Shaarawy SM,Mansour OA,Maduar MA,Khaled HM,El-Ahmadi O

    更新日期:2000-01-01 00:00:00

  • Identification of bacteria directly from positive blood culture samples by DNA pyrosequencing of the 16S rRNA gene.

    abstract::Rapid identification of the causative bacteria of sepsis in patients can contribute to the selection of appropriate antibiotics and improvement of patients' prognosis. Genotypic identification is an emerging technology that may provide an alternative method to, or complement, established phenotypic identification proc...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Motoshima M,Yanagihara K,Morinaga Y,Matsuda J,Hasegawa H,Kohno S,Kamihira S

    更新日期:2012-11-01 00:00:00

  • Auxotyping of Neisseria gonorrhoeae isolated in the United Kingdom.

    abstract::One hundred and twenty consecutive isolates of Neisseria gonorrhoeae from the County of Avon, UK, were examined by auxotyping and penicillin susceptibility testing. Requirements for proline (Pro), arginine (Arg), hypoxanthine (Hyp), uracil (Ura) and methionine (Met) were determined with a modified Heckels' medium. Pro...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Copley CG,Egglestone SI

    更新日期:1983-08-01 00:00:00

  • Effect of fluvastatin and pravastatin, HMG-CoA reductase inhibitors, on fluconazole activity against Candida albicans.

    abstract::Synergy between fluvastatin, at clinically unachievable concentrations, and fluconazole against Candida albicans has been reported. The purpose of the present study was to evaluate the in-vitro activity of fluconazole alone and in combination with clinically achievable concentrations of pravastatin and fluvastatin aga...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Nash JD,Burgess DS,Talbert RL

    更新日期:2002-02-01 00:00:00

  • Virulent Toxoplasma gondii strain RH promotes T-cell-independent overproduction of proinflammatory cytokines IL12 and gamma-interferon.

    abstract::The aim of this study was the analysis of the cytokine response in BALB/c mice infected with the highly virulent RH or the weakly virulent Beverley strains of Toxoplasma gondii. Analysis of cytokine messages showed increased expression of IL12, IFN-gamma and TNF-alpha, but not IL4 mRNAs in spleen cells after infection...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Nguyen TD,Bigaignon G,Markine-Goriaynoff D,Heremans H,Nguyen TN,Warnier G,Delmee M,Warny M,Wolf SF,Uyttenhove C,Van Snick J,Coutelier JP

    更新日期:2003-10-01 00:00:00

  • Replication of Chlamydia trachomatis and C. pneumoniae in the human monocytic cell line U-937.

    abstract::To elucidate whether Chlamydia trachomatis and C. pneumoniae infections occur to a significant extent in monocytes-macrophages, the human monocytic cell line, U-937, was infected with C. trachomatis L2 or C. pneumoniae TW-183. Chlamydial DNA and genus-specific antigens of the lipopolysaccharides (LPS) in epitopes of t...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Numazaki K,Suzuki K,Chiba S

    更新日期:1995-03-01 00:00:00

  • Susceptibilities of Actinobacillus actinomycetemcomitans biofilms to oral antiseptics.

    abstract::The susceptibilities of Actinobacillus actinomycetemcomitans cultures, grown as 1- or 3-day-old biofilms or as planktonic suspensions, to concentrations of chlorhexidine digluconate, cetylpyridinium chloride or triclosan used in commercial mouthwashes were compared. Three-day biofilms were the most resistant form of t...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Thrower Y,Pinney RJ,Wilson M

    更新日期:1997-05-01 00:00:00

  • Non-tuberculous mycobacterial infection of the parotid gland in an immunosuppressed adult.

    abstract::Infections of the parotid gland with non-tuberculous mycobacteria (NTM) are rarely described. Here, we report on an infection of the parotid gland caused by Mycobacterium avium and give a literature-based overview about this entity. In the light of a global increase of mycobacterial infections, unusual manifestations ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Gittinger FS,Raible A,Kempf VAJ

    更新日期:2008-04-01 00:00:00

  • Confirmation of botulism in birds and cattle by the mouse bioassay and Endopep-MS.

    abstract::There have been several outbreaks of botulism among poultry and wild birds in Sweden in recent years. The National Veterinary Institute of Sweden (SVA) has identified botulinum neurotoxin (BoNT)/C1 or the mosaic BoNT/C1D using the mouse bioassay. This is believed to be the first report on the application of the Endope...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Hedeland M,Moura H,Båverud V,Woolfitt AR,Bondesson U,Barr JR

    更新日期:2011-09-01 00:00:00

  • A simple statistical approach that represents the frequency distribution of plasmids in clinical isolates of the enterobacteria.

    abstract::The frequency distribution of plasmids in a representative collection of Escherichia coli and other enterobacteria was compared with the frequencies predicted by the Poisson distribution. The distribution of E. coli plasmids did not differ significantly (p greater than 0.2) whereas the difference between observed and ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Platt DJ

    更新日期:1987-05-01 00:00:00