Comparison of three tonometers for measuring intraocular pressure in rabbits.


PURPOSE:Rabbits are used commonly for the evaluation of drugs and surgery to lower intraocular pressure (IOP). The authors compared the accuracy and variability of three tonometers for measuring IOP in rabbits. METHODS:The anterior chamber of adult rabbits was cannulated with a 25-gauge needle connected to an elevated bottle of balanced salt solution. The bottle was raised and lowered to control IOP. A fluid-filled pressure transducer also was placed in the anterior chamber. Intraocular pressure was decreased in increments of 5 to 10 cm H2O from 50 to 0 mm Hg and was recorded with each of these instruments: the hand-held applanation tonometer, the Tono-pen XL, and the pneumatonometer. RESULTS:The Tono-pen XL and the hand-held applanation tonometer underestimated the IOP, whereas the pneumatonometer slightly overestimated IOP. Under close stopcock conditions, at IOPs between 3 and 30 mm Hg, the Tono-pen XL was as accurate as the pneumatonometer but had a smaller variance; the hand-held applanation tonometer had lower accuracy and higher variability. CONCLUSIONS:The Tono-pen XL is the tonometer of choice for measuring IOP in rabbits within the range of IOP 3 to 30 mm Hg. All tonometers were less accurate when the IOP was elevated markedly.


Abrams LS,Vitale S,Jampel HD


Has Abstract


1996-04-01 00:00:00












  • Latent stereopsis for motion in depth in strabismic amblyopia.

    abstract:PURPOSE:To investigate the residual stereo function of a group of 15 patients with strabismic amblyopia, by using motion-in-depth stimuli that allow discrimination of contributions from local disparity as opposed to those from local velocity mechanisms as a function of the rate of depth change. METHODS:The stereo perf...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章


    authors: Hess RF,Mansouri B,Thompson B,Gheorghiu E

    更新日期:2009-10-01 00:00:00

  • A novel mutation (I143NT) in guanylate cyclase-activating protein 1 (GCAP1) associated with autosomal dominant cone degeneration.

    abstract:PURPOSE:To identify pathogenic mutations in the guanylate cyclase-activating protein 1 (GCAP1) and GCAP2 genes and to characterize the biochemical effect of mutation on guanylate cyclase (GC) stimulation. METHODS:The GCAP1 and GCAP2 genes were screened by direct sequencing for mutations in 216 patients and 421 patient...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章


    authors: Nishiguchi KM,Sokal I,Yang L,Roychowdhury N,Palczewski K,Berson EL,Dryja TP,Baehr W

    更新日期:2004-11-01 00:00:00

  • Heterozygous loss-of-function variants in CYP1B1 predispose to primary open-angle glaucoma.

    abstract:PURPOSE:Although primary congenital glaucoma (PCG)-associated CYP1B1 mutations in the heterozygous state have been evaluated for association with primary open-angle glaucoma (POAG) in several small studies, their contribution to the occurrence of POAG is still controversial. The present study was conducted to determine...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章


    authors: Pasutto F,Chavarria-Soley G,Mardin CY,Michels-Rautenstrauss K,Ingelman-Sundberg M,Fernández-Martínez L,Weber BH,Rautenstrauss B,Reis A

    更新日期:2010-01-01 00:00:00

  • Localized reticular pseudodrusen and their topographic relation to choroidal watershed zones and changes in choroidal volumes.

    abstract:PURPOSE:We identified a topographic relation of localized reticular pseudodrusen (RPD) to choroidal watershed zones (CWZ) and to changes in choroidal volumes (CV). METHODS:We included 30 eyes of 30 patients with RPD in an area <10 mm(2) and no other retinal alteration (76.7 ± 6.9 years). Patients underwent spectral-do...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章


    authors: Alten F,Clemens CR,Heiduschka P,Eter N

    更新日期:2013-05-07 00:00:00

  • Loss of inner retinal neurons after retinal ischemia in rats.

    abstract:PURPOSE:Ischemia is a risk factor for eye diseases like ocular vein occlusion or glaucoma. To investigate effects of ischemia-reperfusion (I/R) a lot of different animal models are used, studying one or two different cell types, which creates heterogeneity of data. The aim of this study was to investigate the function ...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章


    authors: Schmid H,Renner M,Dick HB,Joachim SC

    更新日期:2014-04-28 00:00:00

  • Attenuated glial reactions and photoreceptor degeneration after retinal detachment in mice deficient in glial fibrillary acidic protein and vimentin.

    abstract:PURPOSE:To characterize the reactions of retinal glial cells (astrocytes and Müller cells) to retinal injury in mice that lack glial fibrillary acidic protein (GFAP) and vimentin (GFAP-/-Vim-/-) and to determine the role of glial cells in retinal detachment (RD)-induced photoreceptor degeneration. METHODS:RD was induc...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章


    authors: Nakazawa T,Takeda M,Lewis GP,Cho KS,Jiao J,Wilhelmsson U,Fisher SK,Pekny M,Chen DF,Miller JW

    更新日期:2007-06-01 00:00:00

  • Comparative assessment for the ability of Cirrus, RTVue, and 3D-OCT to diagnose glaucoma.

    abstract:PURPOSE:We compared the ability of circumpapillary retinal nerve fiber layer (cpRNFL) thickness and macular parameters obtained by three spectral-domain optical coherence tomography (SD-OCT) instruments to detect glaucoma. METHODS:We enrolled 87 normal eyes and 145 glaucomatous eyes (75 early glaucomatous eyes (EGs), ...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章


    authors: Akashi A,Kanamori A,Nakamura M,Fujihara M,Yamada Y,Negi A

    更新日期:2013-07-10 00:00:00

  • Removal of biofilm from contact lens storage cases.

    abstract:PURPOSE:Lens case hygiene practices are important in maintaining safe contact lens wear. However, the effectiveness of various lens case cleaning practices have not been evaluated and compared. This in vitro study aimed to evaluate and compare the efficacy of cleaning practices that are most commonly carried out by len...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章


    authors: Wu YT,Zhu H,Willcox M,Stapleton F

    更新日期:2010-12-01 00:00:00

  • Light-Induced Thickening of Photoreceptor Outer Segment Layer Detected by Ultra-High Resolution OCT Imaging.

    abstract:PURPOSE:We examined if light induces changes in the retinal structure that can be observed using optical coherence tomography (OCT). METHODS:Normal C57BL/6J mice (age 3-6 months) adapted to either room light (15 minutes to ∼5 hours, 50-500 lux) or darkness (overnight) were imaged using a Bioptigen UHR-OCT system. Conf...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章


    authors: Li Y,Fariss RN,Qian JW,Cohen ED,Qian H

    更新日期:2016-07-01 00:00:00

  • Efficacy of pig-to-rhesus lamellar corneal xenotransplantation.

    abstract::PURPOSE. To solve the shortage of donor corneas, a decellularizing method based on hypertonic saline treatment was introduced, and a favorable outcome was observed in pig-to-rabbit lamellar corneal transplantation. This study was an investigation of the efficacy of pig-to-nonhuman primate lamellar corneal transplantat...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章


    authors: Choi HJ,Kim MK,Lee HJ,Ko JH,Jeong SH,Lee JI,Oh BC,Kang HJ,Wee WR

    更新日期:2011-08-22 00:00:00

  • Using two preferred retinal loci for different lighting conditions in patients with central scotomas.

    abstract:PURPOSE:Using a scanning laser ophthalmoscope, it was found that some patients with relative central scotomas reliably used two different preferred retinal loci (PRLs) at different stimulus illuminances. This article describes adaptations in a patient's PRL for fixation when dimming the stimulus increased the relative ...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章


    authors: Lei H,Schuchard RA

    更新日期:1997-08-01 00:00:00

  • In vivo effects of 5-FU on ocular surface epithelium following corneal wounding.

    abstract::We evaluated the dose relationship between the antiproliferative and toxic effects of 5-fluorouracil (5-FU) on the ocular surface epithelium following experimental corneal epithelial wounding in rabbits. Central corneal epithelial defects 8 mm in diameter were made using n-heptanol. 5-FU (0.05 mg, 0.5 mg, or 5.0 mg pe...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章


    authors: Capone A Jr,Lance SE,Friend J,Thoft RA

    更新日期:1987-10-01 00:00:00

  • Mislocalization and degradation of human P23H-rhodopsin-GFP in a knockin mouse model of retinitis pigmentosa.

    abstract:PURPOSE:To engineer a knockin mouse model that can be used to monitor the effects of treatments on degradation and mislocalization of proline-to-histidine change at codon 23 (P23H) rhodopsin, a common cause of autosomal dominant retinitis pigmentosa (ADRP). The goal was to introduce a gene that expressed rhodopsin at l...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章


    authors: Price BA,Sandoval IM,Chan F,Simons DL,Wu SM,Wensel TG,Wilson JH

    更新日期:2011-12-28 00:00:00

  • Evaluation of antiproliferative agents using a cell-culture model.

    abstract::Chinese hamster ovarian (CHO) cells in culture were used to evaluate the relative antiproliferative potential of drugs. These agents have been used to improve the clinical response after glaucoma filtering surgery. The following drugs were evaluated: 5-fluorouracil (5-FU) as the benchmark, 5-fluorouridine (FUR), 5-flu...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章


    authors: Senderoff RI,Weber PA,Smith DR,Sokoloski TD

    更新日期:1990-12-01 00:00:00

  • Interaction of the insulin receptor beta-subunit with phosphatidylinositol 3-kinase in bovine ROS.

    abstract:PURPOSE:To identify the tyrosine-phosphorylated protein(s) in bovine rod outer segments (ROS) that are associated with phosphatidylinositol 3-kinase (PI3K). METHODS:Glutathione-S-transferase (GST) fusion proteins containing two SH2 domains of the p85 regulatory subunit of PI3K-GST-p85 (N-SH2), GST-p85 (C-SH2), and res...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章


    authors: Rajala RV,Anderson RE

    更新日期:2001-12-01 00:00:00

  • Biomicroscopic measurement of the optic disc with a high-power positive lens.

    abstract:PURPOSE:To compare the magnification properties of four different indirect double aspheric fundus examination lenses for clinical disc biometry. METHODS:Experimental study in a model eye. The relationship between the true size of a fundus object and its image was calculated for each fundus lens for an ametropic range ...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章


    authors: Ansari-Shahrezaei S,Maar N,Biowski R,Stur M

    更新日期:2001-01-01 00:00:00

  • Intraocular safety and pharmacokinetics of hexadecyloxypropyl-cidofovir (HDP-CDV) as a long-lasting intravitreal antiviral drug.

    abstract:PURPOSE:To evaluate the intraocular safety and pharmacokinetics of hexadecyloxypropyl-cidofovir (HDP-CDV), the hydrolysis product of HDP-cyclic-CDV, a long-lasting intravitreal cidofovir prodrug for cytomegalovirus (CMV) retinitis. METHODS:HDP-cyclic-CDV was suspended in phosphate-buffered saline (PBS) at 37°C and for...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章


    authors: Wang H,Chhablani J,Freeman WR,Beadle JR,Hostetler KY,Hartmann K,Conner L,Aldern KA,Pearson L,Cheng L

    更新日期:2011-12-09 00:00:00

  • Müller cell response to blue light injury of the rat retina.

    abstract:PURPOSE:In addition to photoreceptor degeneration, excessive light causes degenerative alterations in the inner retina and ganglion cell death. A disturbance in osmohomeostasis may be one causative factor for the alterations in the inner retina. Because Müller cells mediate inner retinal osmohomeostasis (mainly through...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章


    authors: Iandiev I,Wurm A,Hollborn M,Wiedemann P,Grimm C,Remé CE,Reichenbach A,Pannicke T,Bringmann A

    更新日期:2008-08-01 00:00:00

  • Monoallelic expression of Krt12 gene during corneal-type epithelium differentiation of limbal stem cells.

    abstract:PURPOSE:The purpose of this study was to characterize a Krt12-Cre knock-in mouse line for corneal epithelium-specific gene ablation and to analyze the allelic selection of the keratin 12 (Krt12) gene during corneal type-epithelium differentiation. METHODS:Knock-in mice were generated by gene targeting. The authors exa...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章


    authors: Hayashi Y,Call MK,Liu CY,Hayashi M,Babcock G,Ohashi Y,Kao WW

    更新日期:2010-09-01 00:00:00

  • Very late antigen-1 mediates corneal lymphangiogenesis.

    abstract:PURPOSE:To investigate the specific role of very late antigen-1 (VLA-1; also known as integrin α1β1) in corneal inflammatory lymphangiogenesis in vivo and lymphatic endothelial cell functions in vitro. METHODS:A standard suture-induced corneal inflammatory lymphangiogenesis model was used in normal adult BALB/c mice t...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章


    authors: Grimaldo S,Yuen D,Ecoiffier T,Chen L

    更新日期:2011-07-01 00:00:00

  • Intravitreal concentrations of a near-infrared fluorescence-labeled biotherapeutic determined in situ using confocal scanning laser ophthalmoscopy.

    abstract:PURPOSE:The pharmacokinetics of ophthalmic biotherapeutics are difficult to determine in human vitreous humor. Because of the high transparency of living tissue to near-infrared (NIR) light, the temporal changes in vitreous concentrations of a biomolecule labeled with an NIR fluorescent probe can be monitored in situ w...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章


    authors: Basile AS,Glazier G,Lee A,Jiang LY,Johnson TR,Shields MJ,Vezina M,Doppalapudi VR

    更新日期:2011-09-01 00:00:00

  • Variations in eye volume, surface area, and shape with refractive error in young children by magnetic resonance imaging analysis.

    abstract:PURPOSE:To determine variations in eye volume, surface area, and shape with refractive error in young children using a three-dimensional magnetic resonance imaging (MRI) model. METHODS:A subset of Singaporean Chinese boys enrolled in the population-based Strabismus, Amblyopia, and Refractive Error in Singapore (STARS)...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章


    authors: Lim LS,Yang X,Gazzard G,Lin X,Sng C,Saw SM,Qiu A

    更新日期:2011-11-17 00:00:00

  • The aging Meibomian gland: an in vivo confocal study.

    abstract:PURPOSE:To evaluate age-related Meibomian gland (MG) changes by in vivo laser scanning confocal microscopy (LSCM). METHODS:Asymptomatic healthy subjects (n=100, age range 20-83 years) with an Ocular Surface Disease Index score of less than 13 were consecutively enrolled. Two additional groups, one composed of subjects...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章


    authors: Villani E,Canton V,Magnani F,Viola F,Nucci P,Ratiglia R

    更新日期:2013-07-16 00:00:00

  • Sequencing arrays for screening multiple genes associated with early-onset human retinal degenerations on a high-throughput platform.

    abstract:PURPOSE:To develop and apply microarray-based resequencing technology to detect sequence alterations in multiple autosomal recessive retinal disease genes on a single high-throughput platform. METHODS:Oligonucleotides corresponding to both strands of the target exons and the flanking intron sequences of 29,214 bp from...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章


    authors: Mandal MN,Heckenlively JR,Burch T,Chen L,Vasireddy V,Koenekoop RK,Sieving PA,Ayyagari R

    更新日期:2005-09-01 00:00:00

  • Morphologic indication for proprioception in the human ciliary muscle.

    abstract:PURPOSE:To search for proprioceptive nerve terminals in human ciliary muscle. METHODS:In 48 human donor eyes, histologic and ultrathin sections cut in different planes and wholemounts of the ciliary muscle were studied. Immunohistochemical staining with antibodies against pan-neuronal antigens and antigens reported as...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章


    authors: Flügel-Koch C,Neuhuber WL,Kaufman PL,Lütjen-Drecoll E

    更新日期:2009-12-01 00:00:00

  • Blue Multifocal Pupillographic Objective Perimetry in Glaucoma.

    abstract:PURPOSE:This study investigated multifocal pupillographic objective perimetry (mfPOP) stimuli that target the intrinsic photosensitivity of melanopsin retinal ganglion cells. The diagnostic potential for glaucoma is compared between stimuli biased toward either cone input to these cells or their melanopsin response. M...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章


    authors: Carle CF,James AC,Kolic M,Essex RW,Maddess T

    更新日期:2015-10-01 00:00:00

  • Antigenic modulation in retinoblastoma: a flow cytometric study.

    abstract::Flow cytometry (FCM) was used to investigate antigenic expression and modulation during the cell cycle of Y-79 and WERI-Rb1 tissue cultured retinoblastoma cell lines using a polyclonal anti-Y-79 antibody and fluorescein conjugated lectins. Several Y-79 resting cell populations were identified by FCM analysis of antibo...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章


    authors: Felberg NT,Augsburger JJ,Shields JA,Goldschmidt J,Pronesti G,Haimowitz A

    更新日期:1985-09-01 00:00:00

  • Effect of optical defocus on detection and recognition of vanishing optotype letters in the fovea and periphery.

    abstract:PURPOSE:Vanishing optotypes (VOs) are pseudo high-pass letters whose mean luminance matches the background so that they "vanish" when the recognition acuity threshold is reached in the fovea. We determined the effect of increasing blur on acuity for these optotypes and conventional letters, in both foveal and extrafove...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章


    authors: Shah N,Dakin SC,Anderson RS

    更新日期:2012-10-09 00:00:00

  • Early multifocal electroretinogram findings during intravitreal ranibizumab treatment for neovascular age-related macular degeneration.

    abstract:PURPOSE:To evaluate changes in the multifocal electroretinogram (mfERG) in patients with neovascular age-related macular degeneration (nAMD) undergoing ranibizumab treatment. METHODS:This was an observational, longitudinal, prospective study. Treatment-naive patients with nAMD who met the inclusion and exclusion crite...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章


    authors: Campa C,Hagan R,Sahni JN,Brown MC,Beare NA,Heimann H,Harding SP

    更新日期:2011-06-01 00:00:00

  • Leukocyte diapedesis in vivo induces transient loss of tight junction protein at the blood-retina barrier.

    abstract:PURPOSE:Although much is now understood about the molecular structure of tight junctions (TJs), little is known about the regulation of their function during neural inflammatory disease processes in vivo. The mechanisms by which leukocytes transmigrate the blood-retina barrier (BRB) without affecting endothelial permea...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章


    authors: Xu H,Dawson R,Crane IJ,Liversidge J

    更新日期:2005-07-01 00:00:00