Transdifferentiated retinal pigment epithelial cells are immunoreactive for vascular endothelial growth factor in surgically excised age-related macular degeneration-related choroidal neovascular membranes.

Abstract:

PURPOSE:To determine the cellular origin and the vascular endothelial growth factor (VEGF) immunoreactivity of the nonvascular stromal cells in surgically excised age-related macular degeneration (ARMD)-associated choroidal neovascular membranes (CNVMs). METHODS:Immunohistochemical analysis was performed on frozen sections of eight surgically excised ARMD-related CNVMs. RESULTS:Cytokeratin-positive, smooth muscle actin-positive polygonal or fibroblastic (transdifferentiated RPE) cells were the principal nonvascular stromal cells detected. The polygonal cells were more commonly found in active (highly vascularized) regions and were strongly immunoreactive for VEGF. The fibroblastic cells were predominantly found in fibrotic (hypovascular) regions and were minimally immunoreactive for VEGF. CONCLUSIONS:Transdifferentiated RPE cells are the principal nonvascular stromal cells of both vascular and fibrotic ARMD-related CNVMs. Preferential localization of VEGF immunoreactivity with the cytoplasm of the polygonal transdifferentiated RPE cells in the highly vascularized regions of the surgically excised CNVMs suggests an important angiogenic role of these cells and this growth factor in the progression of ARMD-related choroidal neovascularization.

authors

Lopez PF,Sippy BD,Lambert HM,Thach AB,Hinton DR

subject

Has Abstract

pub_date

1996-04-01 00:00:00

pages

855-68

issue

5

eissn

0146-0404

issn

1552-5783

journal_volume

37

pub_type

杂志文章
  • Comparison of single versus multiple injections of the protein saratin for prolonging bleb survival in a rabbit model.

    abstract:PURPOSE:We compared the anti-fibrotic effects of single versus multiple postoperative injections of saratin following glaucoma filtration surgery (GFS) in the rabbit model. METHODS:The experiment was in two parts. To determine the optimal frequency for postoperative therapy, seven New Zealand White (NZW) rabbits recei...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.12-10120

    authors: Min J,Lukowski ZL,Levine MA,Meyers CA,Beattie AR,Schultz GS,Samuelson DA,Sherwood MB

    更新日期:2012-11-19 00:00:00

  • Retinal degenerations with truncation mutations in the cone-rod homeobox (CRX) gene.

    abstract:PURPOSE:To define the phenotypes of retinal degenerations associated with mutations in the gene encoding CRX (cone-rod homeobox), a photoreceptor-specific transcription factor. METHODS:Heterozygotes with the E168 [delta1 bp], E168 [delta2 bp], or G217 [delta1 bp] CRXgene mutation were studied clinically, with visual f...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:

    authors: Jacobson SG,Cideciyan AV,Huang Y,Hanna DB,Freund CL,Affatigato LM,Carr RE,Zack DJ,Stone EM,McInnes RR

    更新日期:1998-11-01 00:00:00

  • Different effects of substance P and vasoactive intestinal peptide on the motor function of bovine intraocular muscles.

    abstract::Effects of substance P (SP) and vasoactive intestinal peptide (VIP) on motor function of bovine sphincter, dilator, and ciliary muscles were investigated pharmacologically, with and without field stimulation. SP contracted the sphincter and the maximum response was only about 25% of that in the presence of equimolar d...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:

    authors: Suzuki R,Kobayashi S

    更新日期:1983-12-01 00:00:00

  • Change with age of the refractive index gradient of the human ocular lens.

    abstract:PURPOSE:To study age-related changes in the refractive index distribution of the human ocular lens. METHODS:Biometric data collected on 48 eyes in subjects ranging in age from 19 to 31 years and 48 eyes in subjects ranging in age from 49 to 61 years allowed estimation of a single parameter related to the refractive in...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:

    authors: Hemenger RP,Garner LF,Ooi CS

    更新日期:1995-03-01 00:00:00

  • Disruption of the retinal parafoveal capillary network in type 2 diabetes before the onset of diabetic retinopathy.

    abstract:PURPOSE:To establish, using adaptive optics scanning laser ophthalmoscopy (AOSLO), that the retinal parafoveal capillary network is altered before the onset of diabetic retinopathy in adult patients with type 2 diabetes. METHODS:AOSLO videos were acquired in the parafoveal region of one eye from control subjects and f...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.11-8481

    authors: Tam J,Dhamdhere KP,Tiruveedhula P,Manzanera S,Barez S,Bearse MA Jr,Adams AJ,Roorda A

    更新日期:2011-11-29 00:00:00

  • Absence of degenerative changes in retinal and uveal capillary pericytes in diabetic rats.

    abstract::Ultrastructural morphometric techniques were used to quantify pericyte degeneration in retinal and uveal capillaries of streptozotocin-diabetic rats in order to assess the suitability of this small rodent model of diabetes for studies of the pathogenesis of microvascular eye disease in diabetic humans. Male, Sprague-D...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:

    authors: Tilton RG,LaRose LS,Kilo C,Williamson JR

    更新日期:1986-05-01 00:00:00

  • Dual effects of adenosine on the tone of porcine retinal arterioles in vitro.

    abstract:PURPOSE:Previous studies have shown that adenosine induces relaxation of isolated retinal arterioles mediated by the A2A receptor, but the contributions to tone regulation of adenosine receptors located both in and around the vascular wall have not been studied in detail. METHODS:Porcine retinal arterioles with preser...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.13-13428

    authors: Riis-Vestergaard MJ,Misfeldt MW,Bek T

    更新日期:2014-03-19 00:00:00

  • A biometric study of the eye, orbit, and face in 205 normal human fetuses.

    abstract:PURPOSE:To develop models for assessing the differential growth of the fetal face along its horizontal and vertical axes, as well as of the orbit and the eye. METHODS:Normal human fetuses (n = 205), whose gestational ages ranged from 15.5 to 41 weeks of amenorrhea, were examined. Orbitofacial parameters were as follow...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:

    authors: Denis D,Burguière O,Burillon C

    更新日期:1998-11-01 00:00:00

  • Kallikrein-binding protein levels are reduced in the retinas of streptozotocin-induced diabetic rats.

    abstract:PURPOSE:To determine the involvement of rat kallikrein-binding protein (RKBP) in the development of diabetic retinopathy. METHODS:Diabetes was induced by streptozotocin (STZ) (55 mg/kg body weight in 0.05 M citrate buffer, pH 4.5) in male Sprague-Dawley rats (150 to 175 g, 6 weeks old) as confirmed by hyperglycemia an...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:

    authors: Hatcher HC,Ma JX,Chao J,Chao L,Ottlecz A

    更新日期:1997-03-01 00:00:00

  • Intraocular antibody synthesis during experimental uveitis.

    abstract::Determination of intraocular antibody synthesis against certain microorganisms is a diagnostic aid in identifying the causative agent in clinical uveitis. Little is known, however, concerning the kinetics and specificity of antibodies produced during intraocular inflammation. To investigate this subject we induced uve...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:

    authors: van der Voet JC,Liem A,Otto AJ,Kijlstra A

    更新日期:1989-02-01 00:00:00

  • Electroretinographic anomalies in mice with mutations in Myo7a, the gene involved in human Usher syndrome type 1B.

    abstract:PURPOSE:In humans, mutations in the gene encoding myosin VIIa can cause Usher syndrome type 1b (USH1B), a disease characterized by deafness and retinitis pigmentosa. Myosin VIIa is also the gene responsible for the inner ear abnormalities at the shaker1 (sh1) locus in mice. To date, none of the sh1 alleles examined hav...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:

    authors: Libby RT,Steel KP

    更新日期:2001-03-01 00:00:00

  • Platelets adhering to the vascular wall mediate postischemic leukocyte-endothelial cell interactions in retinal microcirculation.

    abstract:PURPOSE:Recent evidence suggests that platelets play a major role in ischemia-reperfusion injury, not only through thrombus formation but also through participation in inflammatory reactions with leukocytes. This study was designed to investigate the contribution of platelets in leukocyte recruitment to inflamed region...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.03-0526

    authors: Nishijima K,Kiryu J,Tsujikawa A,Miyamoto K,Honjo M,Tanihara H,Nonaka A,Yamashiro K,Katsuta H,Miyahara S,Honda Y,Ogura Y

    更新日期:2004-03-01 00:00:00

  • Effect of protease inhibitors on corneal epithelial migration.

    abstract::Recently protease inhibitors were used to treat corneal ulceration and persistent epithelial defect. An organ culture system was used in this study to examine the effect of four protease inhibitors on rat corneal epithelial migration and on incorporation of 3H-leucine into trichloroacetic acid-precipitable material as...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:

    authors: Zieske JD,Bukusoglu G

    更新日期:1991-06-01 00:00:00

  • Molecular properties of wild-type and mutant betaIG-H3 proteins.

    abstract:PURPOSE:BetaIG-H3 is a TGF-beta-induced cell adhesion molecule, the mutations of which are responsible for a group of 5q31-linked corneal dystrophies. The characteristic findings in these diseases are accumulation of protein deposits of different ultrastructures. To understand the mechanisms of protein deposits in 5q31...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:

    authors: Kim JE,Park RW,Choi JY,Bae YC,Kim KS,Joo CK,Kim IS

    更新日期:2002-03-01 00:00:00

  • Current steering in retinal stimulation via a quasimonopolar stimulation paradigm.

    abstract:PURPOSE:Research to restore some degree of vision to patients suffering from retinal degeneration is becoming increasingly more promising. Several groups have chosen electrical stimulation of the remaining network of a degenerate retina as a means to generate discrete light percepts (phosphenes). Approaches vary signif...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.13-11653

    authors: Matteucci PB,Chen SC,Tsai D,Dodds CW,Dokos S,Morley JW,Lovell NH,Suaning GJ

    更新日期:2013-06-21 00:00:00

  • A mouse model for Sorsby fundus dystrophy.

    abstract:PURPOSE:Sorsby fundus dystrophy (SFD) is a rare, late-onset macular dystrophy caused by mutations in the tissue inhibitor of metalloproteinases-3 (TIMP3) gene. The known mutations introduce potentially unpaired cysteine residues in the C terminus of the protein and result in the formation of higher-molecular-weight pro...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:

    authors: Weber BH,Lin B,White K,Kohler K,Soboleva G,Herterich S,Seeliger MW,Jaissle GB,Grimm C,Reme C,Wenzel A,Asan E,Schrewe H

    更新日期:2002-08-01 00:00:00

  • The effects of digitalis-like compounds on rat lenses.

    abstract:PURPOSE:Fundamental to the maintenance of ionic concentration gradients and transparency of the lens is the activity of Na+,K+-adenosine triphosphatase (ATPase) in the epithelial layer. Recent studies have identified endogenous digitalis-like compounds (DLCs) and 19-norbufalin and its peptide derivatives in human catar...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:

    authors: Lichtstein D,Levy T,Deutsch J,Steinitz M,Zigler JS Jr,Russell P

    更新日期:1999-02-01 00:00:00

  • Alpha2 adrenergic receptor-mediated modulation of cytosolic Ca++ signals at the inner plexiform layer of the rat retina.

    abstract:PURPOSE:Compelling evidence suggests that alpha2 agonists, such as brimonidine, protect retinal ganglion cells (RGCs) from injury in a wide range of animal models. However, the mechanism of action for this protection and the physiological role of the alpha2 adrenergic system in the retina is not well understood. A majo...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.06-0890

    authors: Dong CJ,Guo Y,Wheeler L,Hare WA

    更新日期:2007-03-01 00:00:00

  • Intravascular presence of tumor cells as prognostic parameter in uveal melanoma: a 35-year survey.

    abstract:PURPOSE:Invasion of tumor cells into blood vessels is essential for metastasis of uveal melanoma. The occurrence of ingrowth of tumor cells in blood vessels in uveal melanoma was analyzed, and this parameter was compared with the survival of the patients. METHODS:Between 1972 and 2007, 643 eyes primarily enucleated fo...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.09-3824

    authors: Ly LV,Odish OF,Wolff-Rouendaal Dd,Missotten GS,Luyten GP,Jager MJ

    更新日期:2010-02-01 00:00:00

  • Pharmacokinetics of intravitreal carbenicillin, cefazolin, and gentamicin in rhesus monkeys.

    abstract::Although intravitreal injection of antibiotics is being used more widely in treatment of bacterial endophthalmitis, the pharmacokinetic principles that underlie such therapy have been derived exclusively from experiments in the rabbit. Therefore, we studied several representative antibiotics in normal eyes of rhesus m...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:

    authors: Barza M,Kane A,Baum J

    更新日期:1983-12-01 00:00:00

  • Optic nerve head and peripapillary morphometrics in myopic glaucoma.

    abstract:PURPOSE:To investigate morphological characteristics of optic nerve head and peripapillary region with myopia and glaucoma. METHODS:Ten normal and 17 glaucomatous myopic participants were imaged with a custom 1060-nm swept-source optical coherence tomography system. The three-dimensional images were processed and segm...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.14-14227

    authors: Lee S,Han SX,Young M,Beg MF,Sarunic MV,Mackenzie PJ

    更新日期:2014-06-03 00:00:00

  • Effects of cyclosporine and other immunosuppressive drugs on experimental autoimmune uveoretinitis in rats.

    abstract::Five immunosuppressive and anti-inflammatory agents were tested for their effects on development of experimental autoimmune uveoretinitis (EAU) and immune responses to S-antigen in rats immunized with this retinal antigen. When administered daily from day 0-14 after immunization, cyclosporine at 5-20 mg/Kg was nontoxi...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:

    authors: Mochizuki M,Nussenblatt RB,Kuwabara T,Gery I

    更新日期:1985-02-01 00:00:00

  • Correlation of pseudoexfoliative material and optic nerve damage in pseudoexfoliation syndrome.

    abstract:PURPOSE:To determine whether the severity of glaucomatous damage in eyes with pseudoexfoliative (PEX) glaucoma is related to the amount of PEX material in the trabecular meshwork. METHODS:Trabecular meshwork and optic nerves from 19 eyes (11 donors) with PEX syndrome were studied. Eyes were chosen to represent all sta...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:

    authors: Gottanka J,Flügel-Koch C,Martus P,Johnson DH,Lütjen-Drecoll E

    更新日期:1997-11-01 00:00:00

  • Time Outdoors and Myopia Progression Over 2 Years in Chinese Children: The Anyang Childhood Eye Study.

    abstract:PURPOSE:To investigate whether time outdoors and a range of other activities are associated with change in spherical equivalent (SE) and axial length in Chinese children over a period of 2 years. METHODS:A total of 1997 children aged 12.7 ± 0.5 (10.9-15.6) years in the Anyang Childhood Eye Study (ACES) were examined a...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.14-15474

    authors: Li SM,Li H,Li SY,Liu LR,Kang MT,Wang YP,Zhang F,Zhan SY,Gopinath B,Mitchell P,Wang N,Anyang Childhood Eye Study Group.

    更新日期:2015-07-01 00:00:00

  • Asymmetric Inter-Eye Progression in Stargardt Disease.

    abstract:Purpose:Asymmetry in disease progression between left and right eyes can occur in Stargardt disease (STGD1), and this needs to be considered in novel therapeutic trials with a fellow-eye paired controlled design. This study investigated the inter-eye discordance of best-corrected visual acuity (BCVA) and progression of...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.16-20963

    authors: Lambertus S,Bax NM,Groenewoud JM,Cremers FP,van der Wilt GJ,Klevering BJ,Theelen T,Hoyng CB

    更新日期:2016-12-01 00:00:00

  • Carbonic Anhydrase Inhibitors for the Treatment of Cystic Macular Lesions in Children With X-Linked Juvenile Retinoschisis.

    abstract:Purpose:Little is known regarding the therapeutic effect of carbonic anhydrase inhibitors (CAIs) in the management of cystic macular lesions in children with X-linked juvenile retinoschisis (XLRS) despite the fact that this disease often manifests during childhood. Therefore, our goal was to determine the efficacy of C...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.16-20078

    authors: Verbakel SK,van de Ven JP,Le Blanc LM,Groenewoud JM,de Jong EK,Klevering BJ,Hoyng CB

    更新日期:2016-10-01 00:00:00

  • An assessment of the apex microarray technology in genotyping patients with Leber congenital amaurosis and early-onset severe retinal dystrophy.

    abstract:PURPOSE:Leber congenital amaurosis (LCA) and early-onset severe retinal dystrophy (EOSRD) are genetically heterogeneous, with 11 genes currently implicated. The LCA chip may be used to interrogate many variants in one hybridization reaction. The purpose of this study was to assess the utility of this technology. METHO...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.07-0207

    authors: Henderson RH,Waseem N,Searle R,van der Spuy J,Russell-Eggitt I,Bhattacharya SS,Thompson DA,Holder GE,Cheetham ME,Webster AR,Moore AT

    更新日期:2007-12-01 00:00:00

  • Impact of vitamin A supplementation on prevalence and incidence of xerophthalmia in Nepal.

    abstract:PURPOSE:To assess the impact of vitamin A supplementation at 4-month intervals on the prevalence and incidence of xerophthalmia among preschool-age children. METHODS:A stratified, random sample of 40 wards with 4766 children in Sarlahi district of Nepal was selected to participate in a randomized, controlled, communit...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:

    authors: Katz J,West KP Jr,Khatry SK,Thapa MD,LeClerq SC,Pradhan EK,Pokhrel RP,Sommer A

    更新日期:1995-12-01 00:00:00

  • Mouse conjunctival forniceal gene expression during postnatal development and its regulation by Kruppel-like factor 4.

    abstract:PURPOSE:To identify the changes in postnatal mouse conjunctival forniceal gene expression and their regulation by Klf4 during the eye-opening stage when the goblet cells first appear. METHODS:Laser microdissection (LMD) was used to collect conjunctival forniceal cells from postnatal (PN) day 9, PN14 and PN20 wild-type...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.10-7068

    authors: Gupta D,Harvey SA,Kaminski N,Swamynathan SK

    更新日期:2011-07-01 00:00:00

  • Multiple Differentially Methylated Regions Specific to Keratoconus Explain Known Keratoconus Linkage Loci.

    abstract:Purpose:Keratoconus (KTCN) is a complex eye disorder resulting in loss of visual function. Its development is affected by genetic and environmental components. The aim of this study was to unravel the role of epigenetic factors in KTCN. Methods:To verify if DNA methylation may play a role in KTCN development, reduced ...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.18-25916

    authors: Kabza M,Karolak JA,Rydzanicz M,Udziela M,Gasperowicz P,Ploski R,Szaflik JP,Gajecka M

    更新日期:2019-04-01 00:00:00