Activation of human neutrophils induced by immune complexes prepared with cationic and anionic fractions of normal IgG antibodies.


:The authors have recently shown that the ability of immune complexes (IC) to trigger Fc gamma R-dependent cell responses can be dramatically enhanced when the isoelectric point (pI) of normal IgG antibodies is increased from 5.8-8.5 to 8.5-9.8 by treatment with 1-ethyl-3-2(3-dimethylaminopropyl) carbodiimide HCl and ethylene diamine. In the current work the authors analyse whether differences in the charge of normal IgG antibodies may also affect IC activity. Soluble IC (sIC) were prepared with (a) rabbit IgG antibodies to human IgG and anionic or cationic fractions of human IgG; and (b) bovine serum albumin (BSA) and anionic or cationic fractions of rabbit IgG anti-BSA antibodies. Similar abilities to bind to neutrophil surface were observed for sIC prepared with both anionic (anIC) and cationic fractions of IgG (catIC). Moreover, no differences were found when neutrophil shape change, chemiluminescence (CL) emission and elastase release were induced by either anIC or catIC. As in the case of sIC, particulate IC prepared with erythrocytes (E) and anionic or cationic fractions of specific IgG antibodies (IgG-E) showed no differences in their abilities to trigger either CL emission or ADCC. Taken together, these results suggest that the pI of normal IgG antibodies do not affect the ability of IC to trigger neutrophil responses mediated by receptors for the Fc portion of IgG antibodies (Fc gamma R).


Scand J Immunol


Trevani AS,Andonegui G,Kempfer C,Malchiodi E,Geffner JR




Has Abstract


1996-03-01 00:00:00












  • Upregulated microRNA-132 in T helper 17 cells activates hepatic stellate cells to promote hepatocellular carcinoma cell migration in vitro.

    abstract::MicroRNAs play an important role in the modulation of the immune system. T helper 17 (Th17) cells are involved in the modulation of the tumour microenvironment. However, the function of miRNA in Th17 cells in the tumour microenvironment is unclear. In this study, we analysed miR-132 expression in Th17 cells and assess...

    journal_title:Scandinavian journal of immunology

    pub_type: 杂志文章


    authors: Feng R,Cui Z,Liu Z,Zhang Y

    更新日期:2020-12-02 00:00:00

  • Application of chimerism-based drug-induced tolerance to rat into mouse xenotransplantation.

    abstract::The current critical shortage of human donor organs has stimulated the feasibility of the xenogenic transplantation, such as swine to primate. We have previously reported the induction of donor-specific tolerance in MHC-disparated recipient mice by using our cyclophosphamide (CP)-induced tolerance conditioning. In thi...

    journal_title:Scandinavian journal of immunology

    pub_type: 杂志文章


    authors: Tomita Y,Shimizu I,Iwai T,Zhang QW,Okano S,Kajiwara T,Onzuka T,Tominaga R

    更新日期:2006-10-01 00:00:00

  • A soluble mannose receptor immunoadhesin enhances phagocytosis of Pneumocystis carinii by human polymorphonuclear leukocytes in vitro.

    abstract::Pneumocystis carinii is an opportunistic pathogen that causes pneumonia in immunocompromised hosts. In the normal host, P. carinii is susceptible to an array of first line host defense mechanisms that are operative in the lung. Alveolar macrophages play a central role in the clearance of inhaled organisms. The macroph...

    journal_title:Scandinavian journal of immunology

    pub_type: 杂志文章


    authors: Stehle SE,Rogers RA,Harmsen AG,Ezekowitz RA

    更新日期:2000-08-01 00:00:00

  • Human anti-F(ab')2 antibodies and pepsin agglutinators react with Fv determinants.

    abstract::Affinity-purified human IgG anti-tetanus antibody was subjected to papain and then pepsin digestion, and residual fragments retaining antibody activity were re-isolated by adsorption and elution from Sepharose-tetanus toxoid columns. Both Fab' and Fv fragments isolated by gel filtration showed strong reactivity with a...

    journal_title:Scandinavian journal of immunology

    pub_type: 杂志文章


    authors: Silvestris F,Williams RC Jr,Searles RP

    更新日期:1986-04-01 00:00:00

  • Evidence for a type 1 diabetes susceptibility locus (IDDM10) on chromosome 10p11-q11 in a Russian population.

    abstract::Around 20 susceptibility loci for type 1 diabetes mellitus (T1DM) have been mapped. One of these loci, IDDM10, was found on chromosome 10p11-q11. Here, we investigated whether the IDDM10 locus contributes in the susceptibility to T1DM in a Russian family dataset. One hundred and fourteen simplex Russian families, each...

    journal_title:Scandinavian journal of immunology

    pub_type: 杂志文章


    authors: Chistiakov DA,Seryogin Y,Savost'anov KV,Zilberman LI,Titovich EV,Kuraeva TL,Dedov II,Nosikov VV

    更新日期:2004-09-01 00:00:00

  • Disturbances in the peripheral T-cell repertoire of patients with motor neuron disease: high levels of activation and indirect evidence of superantigen.

    abstract::Our data on peripheral blood T cells from Motor neuron disease (MND) patients indicate major immunological disturbances linked to this disease. Both CD4+ and CD8+ T-cell subsets display an increased fraction of cells showing activation markers compared to controls, indicating an unusually high level of activity in bot...

    journal_title:Scandinavian journal of immunology

    pub_type: 杂志文章


    authors: Katchar K,Osorio L,Conradi S,Wigzell H,Gigliotti D

    更新日期:2001-07-01 00:00:00

  • Characterization of soluble terminal complement complex assembled in C8beta-deficient plasma and serum.

    abstract::Sera genetically deficient in either the alpha-gamma or the beta-subunit of complement component C8 virtually lack haemolytic activity. We have studied the formation and the structural organization of the soluble terminal complement complex (TCC) assembled in these sera following activation with cobra venom factor (CV...

    journal_title:Scandinavian journal of immunology

    pub_type: 杂志文章


    authors: Høgåsen K,Mollnes TE,Nürnberger W,Pausa M,Fukumori Y,Tedesco F

    更新日期:1998-09-01 00:00:00

  • An evaluation of the significance of amino acid sequence homologies in human histocompatibility antigens (HLA-A and HLA-B) with immunoglobulins and other proteins, using relatively short sequences.

    abstract::A computer search was carried out for homologies between HLA-A and HLA-B antigen sequences and the sequences of constant and variable regions of immunoglobulins and of all other sequenced proteins. Searches were made both with relatively short peptide sequences from the HLA antigens and with those longer peptide seque...

    journal_title:Scandinavian journal of immunology

    pub_type: 杂志文章


    authors: Strominger JL,Orr HT,Parham P,Ploegh HL,Mann DL,Bilofsky H,Saroff HA,Wu TT,Kabat EA

    更新日期:1980-01-01 00:00:00

  • Effects of G-CSF on telomere lengths in PBMCs from human immunodeficiency virus-infected patients: results from a randomized, placebo-controlled trial.

    abstract::Telomeres are unique terminal chromosomal structures, the length of which has been shown to decrease with cell division in vitro and with increased age in vivo for human somatic cells. In human immunodeficiency virus (HIV)-1 infection, decrease of telomere length is primarily found in CD8+ T cells, and not in CD4+ T c...

    journal_title:Scandinavian journal of immunology

    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Aladdin H,Ullum H,Schjerling P,Skov Jensen M,Dam Nielsen S,Mathiesen L,Gerstoft J,Skinhøj P,Klarlund Pedersen B

    更新日期:2000-08-01 00:00:00

  • Mechanism of action of suppressor cells. In vivo concanavalin-A-activated suppressor cells do not directly affect B cells.

    abstract::The addition of a small proportion (10%) of in vivo concanavalin-A (Con-A)-activated spleen cells to normal spleen cell cultures suppressed the primary immune response to sheep erythrocytes (SRBC) but had no effect on the thymus-independent primary immune response to 3,5-dinitro-4-hydroxy-phenacetyl-conjugated lipopol...

    journal_title:Scandinavian journal of immunology

    pub_type: 杂志文章


    authors: Ekstedt RD,Waterfield JD,Nespoli L,Möller G

    更新日期:1977-01-01 00:00:00

  • Reactivity patterns of synovial T-cell lines derived from a patient with rheumatoid arthritis. I. Reactions with defined antigens and auto-antigens suggest the existence of multireactive T-cell clones.

    abstract::The functional T-cell repertoire in the inflamed joint of a patient with rheumatoid arthritis was analysed at the clonal level. Using limiting dilution techniques and selecting for growth of in vivo activated and/or autoreactive T cells, 149 T-cell lines were established. They were tested in a proliferation assay for ...

    journal_title:Scandinavian journal of immunology

    pub_type: 杂志文章


    authors: Melchers I,Jooss-Rüdiger J,Peter HH

    更新日期:1997-08-01 00:00:00

  • Extensive CDR3H length heterogeneity exists in bovine foetal VDJ rearrangements.

    abstract::Analysis of seven variable-diversity-joining (VDJ) gene rearrangements in B splenocytes from a 125-day-old bovine foetus revealed an extensive heavy-chain complementarity-determining region 3 (CDR3H) length variation (9-56 codons). Indeed, the global CDR3H size spectratyping of foetal VDJ rearrangements substantiated ...

    journal_title:Scandinavian journal of immunology

    pub_type: 杂志文章


    authors: Saini SS,Kaushik A

    更新日期:2002-02-01 00:00:00

  • Analysis of the T-cell receptor V beta usage in monozygotic and dizygotic twins living in a Plasmodium falciparum endemic area in west Africa.

    abstract::To investigate the influence of genetic and/or environmental factors in the development and shaping of the human peripheral T cell repertoire the authors studied the T-cell receptor (TCR) V beta usage in 10 adult monozygous (Mz) and nine dizygous (Dz) twin pairs living in a Plasmodium falciparum endemic area in West A...

    journal_title:Scandinavian journal of immunology

    pub_type: 杂志文章


    authors: Troye-Blomberg M,Fogdell A,el-Ghazali G,Larsson A,King MH,Sisay-Joof F,Olerup O,Grunewald J,Jepson A

    更新日期:1997-05-01 00:00:00

  • A routine source of human peritoneal macrophages.

    abstract::The routine availability of nucleated human cells for experimental use in limited in the absence of venipuncture. In this paper we have demonstrated that macrophages may be harvested routinely from the waste dialysis bags of patients undergoing continuous ambulatory peritoneal dialysis. These cells were identified as ...

    journal_title:Scandinavian journal of immunology

    pub_type: 杂志文章


    authors: Maddox Y,Foegh M,Zeligs B,Zmudka M,Bellanti J,Ramwell P

    更新日期:1984-01-01 00:00:00

  • H-2 class I Ld antigen positively selects different TCR V beta gene products in CD8+ and CD4+ T cells.

    abstract::Expression of CD4+ or CD8+ determinants and ten different TCR V beta genes was analysed by two-colour flow cytometry in lymph node cells (LNC) of BALB/c and dm2 (a BALB/c Ld loss mutant) mice. TCR V beta 14 expression of CD8+ T cells and TCR V beta 7 and 14 of CD4+ T Cells were significantly more frequent in BALB/c th...

    journal_title:Scandinavian journal of immunology

    pub_type: 杂志文章


    authors: Tscherning T,Reimann J,Claesson MH

    更新日期:1992-10-01 00:00:00

  • A proposed treatment for pathogenic enveloped viruses having high rates of mutation or replication.

    abstract::Several enveloped viruses, particularly some RNA viruses, have high rates of mutation or replication, which can make them virulent pathogens in humans and other mammals. A proposed treatment could use synthesized proteins to mask pathogenic viral surface proteins to quickly induce an immune attack on specific envelope...

    journal_title:Scandinavian journal of immunology

    pub_type: 杂志文章


    authors: Roe K

    更新日期:2020-09-01 00:00:00

  • Heterogeneous expression of recombination activating genes and surface CD5 in CD3low CD4+ CD8+ thymocytes.

    abstract::Clonal selection in the thymus occurs mostly in the CD4+ CD8+ (double positive; DP) stage. Within DP thymocytes, cells in the CD3low subset are believed to be involved in clonal selection, and this subset is generally considered as a homogeneous population. T-cell antigen-receptor (TCR) signals on DP thymocytes are kn...

    journal_title:Scandinavian journal of immunology

    pub_type: 杂志文章


    authors: Sheard M,Sugaya K,Furuta M,Tsuda S,Takahama Y

    更新日期:1996-06-01 00:00:00

  • Enzyme analysis of autologous rosette-forming cells.

    abstract::Autologous rosette-forming cells (ARFC) have been considered to be post-thymic precursor cells. Since thymocytes and peripheral T lymphocytes differ considerably in lactate dehydrogenase isoenzyme pattern and in activity of the enzymes of the purine metabolism, we investigated the enzyme profile in the ARFC. The L-lac...

    journal_title:Scandinavian journal of immunology

    pub_type: 杂志文章


    authors: Plum J,de Smedt M,Sabbe L

    更新日期:1984-03-01 00:00:00

  • Chimeric Epitope Vaccine from Multistage Antigens for Lymphatic Filariasis.

    abstract::Lymphatic filariasis, a mosquito-borne parasitic disease, affects more than 120 million people worldwide. Vaccination for filariasis by targeting different stages of the parasite will be a boon to the existing MDA efforts of WHO which required repeated administration of the drug to reduce the infection level and susta...

    journal_title:Scandinavian journal of immunology

    pub_type: 杂志文章


    authors: Anugraha G,Madhumathi J,Prince PR,Prita PJ,Khatri VK,Amdare NP,Reddy MV,Kaliraj P

    更新日期:2015-10-01 00:00:00

  • Molecular characterization of the porcine TIM-3 gene.

    abstract::TIM-3 is a member of the TIM (T-cell immunoglobulin domain and mucin domain) family, which plays an important role in TH1 responses and autoimmune diseases. In this study, we cloned and characterized the porcine TIM-3 gene. Real-time PCR showed little expression of porcine TIM-3 in muscle and stomach, low expression i...

    journal_title:Scandinavian journal of immunology

    pub_type: 杂志文章


    authors: Zhao W,Li Y,Wang Z,Li K,Yang S

    更新日期:2011-01-01 00:00:00

  • Suppressed expression of homotypic multinucleation, extracellular domains of CD172α (SIRP-α) and CD47 (IAP) receptors in TAMs upregulated by Hsp70-peptide complex in Dalton's lymphoma.

    abstract::CD172α and CD47 are members of glycoprotein expressed on macrophages and various immune cells, promote immune recognition and T cell stimulation that priming phagocytosis of pathogens and apoptotic bodies and malignant cell. Tumour-releasing immunosuppressive factor promotes tumour growth and transforms the tumour res...

    journal_title:Scandinavian journal of immunology

    pub_type: 杂志文章


    authors: Gautam PK,Acharya A

    更新日期:2014-07-01 00:00:00

  • Analysis of properties and proinflammatory functions of cockroach allergens Per a 1.01s.

    abstract::Cockroaches have been identified as one of the major indoor allergens inducing perennial rhinitis and asthma. Per a 1s are a group of the major allergens from American cockroach. Although Per a 1s are major allergens from American cockroach, factors contributing to the allergenicity of Per a 1s are still poorly define...

    journal_title:Scandinavian journal of immunology

    pub_type: 杂志文章


    authors: He S,Zhang Z,Zhang H,Wei J,Yang L,Yang H,Sun W,Zeng X,Yang P

    更新日期:2011-09-01 00:00:00

  • Transcriptional regulation of serum amyloid A1 gene expression in human aortic smooth muscle cells involves CCAAT/enhancer binding proteins (C/EBP) and is distinct from HepG2 cells.

    abstract::Regulation of acute-phase serum amyloid A (A-SAA) synthesis by proinflammatory cytokines and steroid hormones in human aortic smooth muscle cells (HASMCs) is distinct from that in HepG2 cells. To study the cis- and trans-activating promoter element involved in the SAA1 gene expression by HASMCs and HepG2 cells, we con...

    journal_title:Scandinavian journal of immunology

    pub_type: 杂志文章


    authors: Kumon Y,Suehiro T,Faulkes DJ,Hosakawa T,Ikeda Y,Woo P,Sipe JD,Hashimoto K

    更新日期:2002-11-01 00:00:00

  • Disruption of nocturnal melatonin rhythm and immunological involvement in ischaemic stroke patients.

    abstract::The aim of this study was to evaluate nocturnal and diurnal urinary melatonin excretion in a group of ischaemic stroke patients, and to verify if a possible impairment of this excretion could be linked with some changes of immunological and neuro-psychic status. We assessed 13 ischaemic stroke patients and five health...

    journal_title:Scandinavian journal of immunology

    pub_type: 杂志文章


    authors: Fiorina P,Lattuada G,Silvestrini C,Ponari O,Dall'Aglio P

    更新日期:1999-08-01 00:00:00

  • Irregular cytokine pattern of CD4+ T lymphocytes in response to Staphylococcus aureus in patients with Wegener's granulomatosis.

    abstract::The initial stage of Wegener's Granulomatosis (WG) is often marked by symptoms of infection and it has been postulated that a bacterial infection could be the aetiologic factor of this disease. The objective of our work was to investigate T-cell-mediated immunity in WG by testing proliferative responses on bacterial a...

    journal_title:Scandinavian journal of immunology

    pub_type: 杂志文章


    authors: Mayet WJ,Märker-Hermann E,Schlaak J,Meyer Zum Büschenfelde KH

    更新日期:1999-06-01 00:00:00

  • Non-major histocompatibility complex dependent variations in lymphocyte activity between inbred mouse strains susceptible to various autoimmune diseases.

    abstract::Transgenic techniques in inbred mouse strains are powerful tools to investigate the specific roles of genes in biological pathways and disease models. However, there is increasing concern over the influence of a variable genetic background in such experiments. To date there have been few investigations of the immunolo...

    journal_title:Scandinavian journal of immunology

    pub_type: 杂志文章


    authors: Johansson AC,Vestberg M,Holmdahl R

    更新日期:2000-07-01 00:00:00

  • DNA vaccine encoding ESAT-6 enhances the protective efficacy of BCG against Mycobacterium tuberculosis infection in mice.

    abstract::ESAT-6 is a protein produced by virulent Mycobacterium tuberculosis but absent in Mycobacterium bovis bacillus Calmette-Guerin (BCG) and is considered as a promising vaccine subunit against tuberculosis. The protective efficacy of a new strategy based on the combination of DNA vaccine encoding ESAT-6 (DNA-E6) and BCG ...

    journal_title:Scandinavian journal of immunology

    pub_type: 杂志文章


    authors: Fan X,Gao Q,Fu R

    更新日期:2007-11-01 00:00:00

  • Fibronectin binds to complement-coated agarose beads and increases their association to mouse macrophages.

    abstract::We have studied the binding of fibronectin to complement (C3b, C3bi, C3d)-coated agarose beads and its effect on cell association of such beads to mouse macrophages. Fibronectin bound to agarose beads preincubated in human serum, whereas no binding occurred after preincubation of the beads with complement-inactivated ...

    journal_title:Scandinavian journal of immunology

    pub_type: 杂志文章


    authors: Johnson E,Gauperaa T,Eskeland T

    更新日期:1985-09-01 00:00:00

  • Synthesis of cytokines during tumour development in mice immunized with the mycobacterial antigen complex A60.

    abstract::The authors have previously reported on the ability of A60, an immunodominant antigenic complex of Mycobacterium bovis BCG, to prevent cancer development in mice challenged with EMT 6 tumour cells. Such effect proved to rely on neoplastic cell lysis by cytolytic T lymphocytes and activated macrophages. The involvement...

    journal_title:Scandinavian journal of immunology

    pub_type: 杂志文章


    authors: Maes H,Cocito C

    更新日期:1996-10-01 00:00:00

  • Feedback regulation of mitochondria by caspase-9 in the B cell receptor-mediated apoptosis.

    abstract::During the germinal centre reaction (GC), B cells with non-functional or self-reactive antigen receptors are negatively selected by apoptosis to generate B cell repertoire with appropriate antigen specificities. We studied the molecular mechanism of Fas/CD95- and B cell receptor (BCR)-induced apoptosis to shed light o...

    journal_title:Scandinavian journal of immunology

    pub_type: 杂志文章


    authors: Eeva J,Nuutinen U,Ropponen A,Mättö M,Eray M,Pellinen R,Wahlfors J,Pelkonen J

    更新日期:2009-12-01 00:00:00