Activation of human neutrophils induced by immune complexes prepared with cationic and anionic fractions of normal IgG antibodies.


:The authors have recently shown that the ability of immune complexes (IC) to trigger Fc gamma R-dependent cell responses can be dramatically enhanced when the isoelectric point (pI) of normal IgG antibodies is increased from 5.8-8.5 to 8.5-9.8 by treatment with 1-ethyl-3-2(3-dimethylaminopropyl) carbodiimide HCl and ethylene diamine. In the current work the authors analyse whether differences in the charge of normal IgG antibodies may also affect IC activity. Soluble IC (sIC) were prepared with (a) rabbit IgG antibodies to human IgG and anionic or cationic fractions of human IgG; and (b) bovine serum albumin (BSA) and anionic or cationic fractions of rabbit IgG anti-BSA antibodies. Similar abilities to bind to neutrophil surface were observed for sIC prepared with both anionic (anIC) and cationic fractions of IgG (catIC). Moreover, no differences were found when neutrophil shape change, chemiluminescence (CL) emission and elastase release were induced by either anIC or catIC. As in the case of sIC, particulate IC prepared with erythrocytes (E) and anionic or cationic fractions of specific IgG antibodies (IgG-E) showed no differences in their abilities to trigger either CL emission or ADCC. Taken together, these results suggest that the pI of normal IgG antibodies do not affect the ability of IC to trigger neutrophil responses mediated by receptors for the Fc portion of IgG antibodies (Fc gamma R).


Scand J Immunol


Trevani AS,Andonegui G,Kempfer C,Malchiodi E,Geffner JR




Has Abstract


1996-03-01 00:00:00












  • Stimulation of the same B-cell population by thymus-independent dextran and by thymus-dependent oligosaccharide-carrier.

    abstract::The immune response of adult BALB/c mice against the bacterial antigen dextran B1355S (Dex) is well characterized as thymus independent (TI type 2) and Igha linked. The antisera consist of mainly IgM/lambda antibodies directed against the alpha(1-->3) glucosidic linkage. This study describes the immune response agains...

    journal_title:Scandinavian journal of immunology

    pub_type: 杂志文章


    authors: Willers J,Weiler E,Kolb C

    更新日期:1995-09-01 00:00:00

  • Properties of purified papain-solubilized rat AgB antigens and reactivity of a xenoantiserum against the isolated antigens.

    abstract::Rat AgB transplantation antigens were isolated after papain digestion of spleens from the inbred strain Hooded Lister. Both subunits of the AgB antigens were present in the purified material. Some physical characteristics of the antigens have been determined. An antiserum, raised in a rabbit, against the purified mate...

    journal_title:Scandinavian journal of immunology

    pub_type: 杂志文章


    authors: Kvist S,Eriksson U,Peterson PA

    更新日期:1980-01-01 00:00:00

  • The T cell receptor and AIDS pathogenesis.

    abstract::An idiotypic network model of AIDS pathogenesis is described in which the T cell receptor plays a role both in infection and as a target of autoimmunity. This is an extension of a previously published autoimmunity model, and provides explanations for several otherwise puzzling aspects of AIDS pathogenesis. In the mode...

    journal_title:Scandinavian journal of immunology

    pub_type: 杂志文章


    authors: Hoffmann GW

    更新日期:1995-04-01 00:00:00

  • The role of I-A/E molecules in B-lymphocyte activation. II. Mechanism of inhibition of the responses to lipopolysaccharide by anti-I-A/E antibodies.

    abstract::We have previously demonstrated that monoclonal anti-I-A/E antibodies inhibit B-cell responses to lipopolysaccharide (LPS). In the present report, the inhibitory effects were shown to be carried out directly on B cells, and to be totally independent of the LPS concentration used, thereby showing that antibodies do not...

    journal_title:Scandinavian journal of immunology

    pub_type: 杂志文章


    authors: Forsgren S,Martinez-A C,Coutinho A

    更新日期:1987-03-01 00:00:00

  • Isolation and identification of the naturally occurring, newborn spleen-associated suppressor cells. A mixed monocyte/mast cell population with separable suppressor activities.

    abstract::Spleens from newborn mice less than 6-7 days of age contain 'naturally occurring' suppressor cell populations that are able to inhibit nonspecifically immune responses of third-party adult spleen cells and alloreactivity in the newborn spleen per se. Isolation of the effectors of this suppressor activity reveals that ...

    journal_title:Scandinavian journal of immunology

    pub_type: 杂志文章


    authors: Peeler K,Wigzell H,Peck AB

    更新日期:1983-05-01 00:00:00

  • A soluble mannose receptor immunoadhesin enhances phagocytosis of Pneumocystis carinii by human polymorphonuclear leukocytes in vitro.

    abstract::Pneumocystis carinii is an opportunistic pathogen that causes pneumonia in immunocompromised hosts. In the normal host, P. carinii is susceptible to an array of first line host defense mechanisms that are operative in the lung. Alveolar macrophages play a central role in the clearance of inhaled organisms. The macroph...

    journal_title:Scandinavian journal of immunology

    pub_type: 杂志文章


    authors: Stehle SE,Rogers RA,Harmsen AG,Ezekowitz RA

    更新日期:2000-08-01 00:00:00

  • The LFA-3 adhesion pathway is differently utilized by superantigen-activated human CD4+ T-cell subsets.

    abstract::The superantigen SEA binds to MHC class II molecules and activates a large fraction of T cells as a result of interaction with particular TCR-V beta sequences. MHC class II transfected CHO cells induce a marginal CD4+ T-cell proliferation in the presence of SEA. CHO cells transfected with both MHC class II and LFA-3 (...

    journal_title:Scandinavian journal of immunology

    pub_type: 杂志文章


    authors: Gjörloff A,Hedlund G,Kalland T,Sansom D,Fischer H,Trowsdale J,Sjögren HO,Dohlsten M

    更新日期:1992-08-01 00:00:00

  • The intravenous model of murine tuberculosis is less pathogenic than the aerogenic model owing to a more rapid induction of systemic immunity.

    abstract::The detection of mRNA in the murine model of tuberculosis for key cytokines involved in protective immunity in the lung tissues revealed a much faster emergence of the interferon (IFN)-gamma response in the intravenous route than in the aerosol route of inoculation. This slower response in the lungs was associated wit...

    journal_title:Scandinavian journal of immunology

    pub_type: 杂志文章


    authors: Cardona PJ,Cooper A,Luquín M,Ariza A,Filipo F,Orme IM,Ausina V

    更新日期:1999-04-01 00:00:00

  • Intrinsic cell membrane antigens recognized by antichromatin autoantibodies. The membrane antigens do not derive from the nucleus.

    abstract::The main object of this study was to see whether or not chromatin constituents are present in cell membranes. The binding of antinuclear antibodies (ANA) to plasma membranes of leucocytes was studied by using autoantibodies and induced antibodies to different histones and histone peptides, and autoantibodies to double...

    journal_title:Scandinavian journal of immunology

    pub_type: 杂志文章


    authors: Rekvig OP

    更新日期:1989-01-01 00:00:00

  • Ia-expressing cells and T lymphocytes of different subsets in peripheral nerve tissue during experimental allergic neuritis in Lewis rats.

    abstract::Inflammatory infiltrates in sciatic nerves during the acute phase of experimental allergic neuritis in the Lewis rat have been characterized with regard to occurrence and distribution of Ia-expressing cells and T-lymphocyte subsets by the help of an immunohistochemical double-staining technique, enabling the simultane...

    journal_title:Scandinavian journal of immunology

    pub_type: 杂志文章


    authors: Olsson T,Holmdahl R,Klareskog L,Forsum U

    更新日期:1983-10-01 00:00:00

  • Alpha 1-microglobulin is mitogenic for guinea pig lymphocytes.

    abstract::Guinea pig alpha 1-microglobulin (alpha 1-m) was found to exert mitogenic effects on guinea pig lymphocytes, i.e. peritoneal exudate lymphocytes (PEL) or T lymphocytes from regional or mesenteric lymph nodes (LNL). On the other hand, spleen T lymphocytes did not respond to alpha 1-m. Stimulation of LNL required the pr...

    journal_title:Scandinavian journal of immunology

    pub_type: 杂志文章


    authors: Lögdberg L,Akerström B,Shevach EM

    更新日期:1986-11-01 00:00:00

  • Suppressed expression of homotypic multinucleation, extracellular domains of CD172α (SIRP-α) and CD47 (IAP) receptors in TAMs upregulated by Hsp70-peptide complex in Dalton's lymphoma.

    abstract::CD172α and CD47 are members of glycoprotein expressed on macrophages and various immune cells, promote immune recognition and T cell stimulation that priming phagocytosis of pathogens and apoptotic bodies and malignant cell. Tumour-releasing immunosuppressive factor promotes tumour growth and transforms the tumour res...

    journal_title:Scandinavian journal of immunology

    pub_type: 杂志文章


    authors: Gautam PK,Acharya A

    更新日期:2014-07-01 00:00:00

  • Interaction between Borrelia burgdorferi and immature human dendritic cells.

    abstract::Antigen uptake and the following maturation of dendritic cells (DCs) are pivotal to the initiation of specific antimicrobial immune responses. DCs also play an important role in the recruitment and activation of the cells of the innate immune system. We have examined the interactions of DCs with Borrelia burgdorferi t...

    journal_title:Scandinavian journal of immunology

    pub_type: 杂志文章


    authors: Suhonen J,Komi J,Soukka J,Lassila O,Viljanen MK

    更新日期:2003-07-01 00:00:00

  • Galectin-3 is associated with severe heart failure and death: A hospital-based study in Chinese patients.

    abstract::Heart failure (HF) is a serious disease syndrome characterized by elevated pro-inflammatory cytokines and inflammatory mediators presume to have significant contribution on disease progression. Galectins are carbohydrate-binding proteins responsible of various physiological functions. Role of galectins in heart failur...

    journal_title:Scandinavian journal of immunology

    pub_type: 杂志文章


    authors: Wang N,Dang M,Zhang W,Lei Y,Liu Z

    更新日期:2020-05-01 00:00:00

  • The effects of topical treatment with steroids or dithranol on epidermal T lymphocytes and dendritic cells in psoriasis.

    abstract::We describe the effects of treatment with topical steroids or dithranol on T and dendritic cells in the skin of patients with chronic plaque psoriasis. Resolution of lesions by both types of topical treatments was accompanied by a marked decrease in epidermal T cells. In steroid treated lesions there was also a reduct...

    journal_title:Scandinavian journal of immunology

    pub_type: 杂志文章


    authors: Baker BS,Swain AF,Griffiths CE,Leonard JN,Fry L,Valdimarsson H

    更新日期:1985-11-01 00:00:00

  • Molecular characterization of the porcine TIM-3 gene.

    abstract::TIM-3 is a member of the TIM (T-cell immunoglobulin domain and mucin domain) family, which plays an important role in TH1 responses and autoimmune diseases. In this study, we cloned and characterized the porcine TIM-3 gene. Real-time PCR showed little expression of porcine TIM-3 in muscle and stomach, low expression i...

    journal_title:Scandinavian journal of immunology

    pub_type: 杂志文章


    authors: Zhao W,Li Y,Wang Z,Li K,Yang S

    更新日期:2011-01-01 00:00:00

  • Isolation and characterization of a kappa amyloid fibril protein.

    abstract::The fibril in primary amyloidosis (AL) is composed of a monoclonal light chain or portions thereof. No unique primary structure has been identified that predisposes certain light chains to form amyloid fibrils. Currently, classification of amyloidosis is based on the biochemistry of the amyloid fibril. We determined t...

    journal_title:Scandinavian journal of immunology

    pub_type: 杂志文章


    authors: Gertz MA,Skinner M,Cohen AS,Connors LH,Kyle RA

    更新日期:1985-09-01 00:00:00

  • DNA vaccine encoding ESAT-6 enhances the protective efficacy of BCG against Mycobacterium tuberculosis infection in mice.

    abstract::ESAT-6 is a protein produced by virulent Mycobacterium tuberculosis but absent in Mycobacterium bovis bacillus Calmette-Guerin (BCG) and is considered as a promising vaccine subunit against tuberculosis. The protective efficacy of a new strategy based on the combination of DNA vaccine encoding ESAT-6 (DNA-E6) and BCG ...

    journal_title:Scandinavian journal of immunology

    pub_type: 杂志文章


    authors: Fan X,Gao Q,Fu R

    更新日期:2007-11-01 00:00:00

  • Regulation of fatty acid synthesis in immune cells.

    abstract::Metabolic reprogramming plays a critical role in the important cellular metabolic alterations that occur during the activation of immune cells to enable them to adapt to the extracellular environment. Here, we review recent studies on how substrate availability and metabolites mediate the signalling pathways that regu...

    journal_title:Scandinavian journal of immunology

    pub_type: 杂志文章,评审


    authors: Qian X,Yang Z,Mao E,Chen E

    更新日期:2018-11-01 00:00:00

  • Down-regulation of interleukin-12, interleukin-12R expression/activity mediates the switch from Th1 to Th2 granuloma response during murine Schistosomiasis mansoni.

    abstract::In murine schistosomiasis mansoni the worm egg-induced granulomatous inflammation is bi-phasic: an initial Th1 type is subsequently switched to a Th2 type response. Analysis of the cellular, molecular base of the Th1-associated response (5-6 weeks post infection) revealed mRNA messages for interleukin (IL)-12 p40, IL-...

    journal_title:Scandinavian journal of immunology

    pub_type: 杂志文章


    authors: Todt JC,Whitfield JR,Ivard SR,Boros DL

    更新日期:2000-10-01 00:00:00

  • Enzymatic fragmentation of an unusual human IgG2 (Kva) myeloma protein.

    abstract::The Kva IgG2(k) myeloma protein showed a complete resistance to papain in the presence of cysteine at neutral pH, and a higher resistance to trypsin and alpha-chymotrypsin digestion than other IgG2 proteins. On the other hand, the Kva molecule was cleaved by pepsin at low pH to give the expected F(ab')2 fragment. When...

    journal_title:Scandinavian journal of immunology

    pub_type: 杂志文章


    authors: Vogt RA,Michaelsen TE

    更新日期:1987-07-01 00:00:00

  • Atrial fibrillation: inflammation in disguise?

    abstract::Atrial fibrillation is highly prevalent, and affected patients are at an increased risk of a number of complications, including heart failure and thrombo-embolism. Over the past years, there has been increasing interest in the role of inflammatory processes in atrial fibrillation, from the first occurrence of the arrh...

    journal_title:Scandinavian journal of immunology

    pub_type: 杂志文章,评审


    authors: Lappegård KT,Hovland A,Pop GA,Mollnes TE

    更新日期:2013-08-01 00:00:00

  • T-cell epitope mapping of the Borrelia garinii outer surface protein A in lyme neuroborreliosis.

    abstract::We studied the T-cell reactivity to overlapping peptides of B. garinii OspA, in order to locate possible immunodominant T-cell epitopes in neuroborreliosis. Cells from cerebrospinal fluid (CSF) and blood from 39 patients with neuroborreliosis and 31 controls were stimulated with 31 overlapping peptides, and interferon...

    journal_title:Scandinavian journal of immunology

    pub_type: 杂志文章


    authors: Widhe M,Ekerfelt C,Jarefors S,Skogman BH,Peterson EM,Bergström S,Forsberg P,Ernerudh J

    更新日期:2009-08-01 00:00:00

  • Immunoglobulin A and CD8 T-cell mucosal immune defenses protect against intranasal infection with Chlamydia pneumoniae.

    abstract::Chlamydia pneumoniae initiates infection in humans via the mucosal epithelia of the respiratory tract; therefore, immunity at this mucosal site is believed to be important to control infection with this pathogen. We compared the protective capacity of immunization in mice with two C. pneumoniae antigens, namely the ma...

    journal_title:Scandinavian journal of immunology

    pub_type: 杂志文章


    authors: Rodríguez A,Rottenberg M,Tjärnlund A,Fernández C

    更新日期:2006-03-01 00:00:00

  • Factors Influencing Serum Concentrations of Immunoglobulin D in the Adult Population: An Observational Study in Spain.

    abstract::Immunoglobulin D (IgD) is the least studied of immunoglobulin classes. This study sought to investigate the potential relationship between demographic, metabolic, lifestyle and immunological factors, and serum IgD concentrations in a general adult population. We measured serum IgD concentrations by means of a commerci...

    journal_title:Scandinavian journal of immunology

    pub_type: 杂志文章


    authors: Carballo I,Rabuñal N,Alvela L,Pérez LF,Vidal C,Alonso M,Sopeña B,Gude F,Gonzalez-Quintela A

    更新日期:2017-04-01 00:00:00

  • Contribution of stabilizing agents present in intravenous immunoglobulin preparations to modulation of mononuclear cell proliferation in vitro.

    abstract::Intravenous immunoglobulin (IVIG) preparations have been shown to suppress lymphocyte proliferation in vitro. This study aimed to investigate the effects of an IVIG produced by fractionation/DEAE-Sephadex adsorption on lymphocyte proliferation in vitro, with particular reference to contributions of the stabilizing age...

    journal_title:Scandinavian journal of immunology

    pub_type: 杂志文章


    authors: Alder LB,Morgan LA,Spickett GP

    更新日期:1996-12-01 00:00:00

  • The chemotactic activity of normal and complement-activated serum as measured by the leading-front method using a Boyden chamber.

    abstract::The chemotactic activity in serum, defined as the attractant effect of serum on the migration of neutrophil granulocytes (PMN) has been investigated for the purpose of characterizing the major chemotactic factors in serum as measured by the leading-front technique, using a modified Boyden chamber. The chemotactic acti...

    journal_title:Scandinavian journal of immunology

    pub_type: 杂志文章


    authors: Håkansson L,Venge P

    更新日期:1984-01-01 00:00:00

  • Acute phase serum amyloid A protein increases high density lipoprotein binding to human peripheral blood mononuclear cells and an endothelial cell line.

    abstract::Serum Amyloid A (SAA) is an acute-phase protein secreted mainly by hepatocytes and is largely associated with high-density lipoprotein (HDL) in plasma. It has been suggested that SAA alters HDL binding to the cell surface and that this in turn changes HDL-mediated cholesterol delivery to cells. Incorporation of SAA in...

    journal_title:Scandinavian journal of immunology

    pub_type: 杂志文章


    authors: Hayat S,Raynes JG

    更新日期:2000-02-01 00:00:00

  • Inhibitory effect of haptoglobin on granulocyte chemotaxis, phagocytosis and bactericidal activity.

    abstract::Human haptoglobin (Hp) is synthesized at hepatic and extrahepatic sites as an acute-phase reactant protein (APP). We investigated the effects of Hp on granulocyte function. The chemotaxis of freshly isolated human granulocytes and differentiated HL-60 cells in response to the bacterial tripeptide, f-met-leu-phe, was i...

    journal_title:Scandinavian journal of immunology

    pub_type: 杂志文章


    authors: Rossbacher J,Wagner L,Pasternack MS

    更新日期:1999-10-01 00:00:00

  • A novel antibody directed against a three-dimensional configuration of a 95-kDa protein in patients with autoimmune hepatic diseases.

    abstract::Sera from patients with primary biliary cirrhosis recognize various cellular components, such as mitochondria, centromere, nuclear envelope, and multiple nuclear dot antigens. There also appears to be a novel antibody reacting with a particular protein in these sera. The presence of this antibody was investigated by d...

    journal_title:Scandinavian journal of immunology

    pub_type: 杂志文章


    authors: Miyachi K,Matsushima H,Hankins RW,Hirakata M,Mimori T,Hosaka H,Amagasaki Y,Miyakawa H,Kako M,Shibata M,Onozuka Y,Ueno U

    更新日期:1998-01-01 00:00:00