Chronic inhalation exposure to ozone and nitric acid elevates stress-inducible heat shock protein 70 in the rat lung.


:The ability of urban oxidant and acid air pollutants to induce heat shock proteins (HSPs) in the mammalian lung is not known. Such proteins are known to be correlated with environmental stress and pathophysiological conditions. In this study, stress-inducible HSP 70 was assessed by slot-blotting in rat lungs (N=10 per group) following inhalation exposures for 4 h per day, 3 days per week for 40 weeks to the following pollutants: (a) purified air;(b) 0.15 ppm ozone (O3);(c)50 micrograms/m3 nitric acid (HNO3); or(d) a combination of both 0.15 ppm O3 and 50 micrograms/m3 HNO3. At 24 h following the last exposure, samples from the right apical lobe of the lung were obtained for either slot-blotting or gel electrophoretic separation, subsequent protein immunoblotting, and chemiluminescence detection of HSP 70 levels. Experiments demonstrate that stress-inducible HSP 70 was present constitutively in the control lungs and was separable from the constitutive form of HSP 70. Slot-blotting analysis demonstrate that the O3 and HNO3 exposures alone produced significant elevations of HSP70. Specifically, either O3 or HNO3 alone significantly elevated lung stress-inducible HSP 70 levels by 277% and 221% respectively, above control levels. The group exposed to combined O3 and HNO3 showed a 177% elevation in lung stress-inducible HSP 70 that was significantly greater that the group inhaling purified air, but this effect was less than the effects of either pollutant component alone. Moreover, all exposure groups were significantly different from one another. These results indicate that stress-inducible HSP 70 in the rat lung is highly elevated after chronic inhalation exposures to both O3 and HNO3 when administered either alone or in combination within the range of urban ambient concentrations.






Wong CG,Bonakdar M,Mautz WJ,Kleinman MT




Has Abstract


1996-02-22 00:00:00














  • Low level prenatal exposure to methylmercury disrupts neuronal migration in the developing rat cerebral cortex.

    abstract::We determined the effects of low-level prenatal MeHg exposure on neuronal migration in the developing rat cerebral cortex using in utero electroporation. We used offspring rats born to dams that had been exposed to saline or various doses of MeHg (0.01 mg/kg/day, 0.1 mg/kg/day, and 1 mg/kg/day) from gestational day (G...


    pub_type: 杂志文章


    authors: Guo BQ,Yan CH,Cai SZ,Yuan XB,Shen XM

    更新日期:2013-02-08 00:00:00

  • Influence of 2,3-dimercaptopropane-1-sulfonate and dimercaptosuccinic acid on the mobilization of mercury from tissues of rats pretreated with mercuric chloride, phenylmercury acetate or mercury vapors.

    abstract::The efficiency of the sodium salt of 2,3-dimercaptopropanesulfonic acid (DMPS) and meso-dimercaptosuccinic acid (DMSA) to mobilize mercury from tissues has been assessed in rats pretreated with different doses of HgCl2, phenylmercury acetate or exposed to different concentrations of mercury vapors. These pretreatments...


    pub_type: 杂志文章


    authors: Buchet JP,Lauwerys RR

    更新日期:1989-03-01 00:00:00

  • An epigrammatic (abridged) recounting of the myriad tales of astonishing deeds and dire consequences pertaining to nitric oxide and reactive oxygen species in mitochondria with an ancillary missive concerning the origins of apoptosis.

    abstract::Mitochondria play a central role in the life and death of cells. These organelles serve as the major energy-producing power-house, whereby the generation of ATP is associated with the utilization of molecular oxygen. A significant fraction (2-3%) of molecular oxygen consumed by mitochondria may be reduced in a one-ele...


    pub_type: 杂志文章,评审


    authors: Heck DE,Kagan VE,Shvedova AA,Laskin JD

    更新日期:2005-03-15 00:00:00

  • Sodium valproate induces mitochondrial respiration dysfunction in HepG2 in vitro cell model.

    abstract::Sodium valproate (VPA) is a potentially hepatotoxic antiepileptic drug. Risk of VPA-induced hepatotoxicity is increased in patients with mitochondrial diseases and especially in patients with POLG1 gene mutations. We used a HepG2 cell in vitro model to investigate the effect of VPA on mitochondrial activity. Cells wer...


    pub_type: 杂志文章


    authors: Komulainen T,Lodge T,Hinttala R,Bolszak M,Pietilä M,Koivunen P,Hakkola J,Poulton J,Morten KJ,Uusimaa J

    更新日期:2015-05-04 00:00:00

  • Studies on the mechanism of paracetamol-induced protection against paracetamol hepatotoxicity.

    abstract::In rats, 3 days treatment with paracetamol (1 oral dose of 1 g/kg daily) produced a complete protection against the hepatotoxic actions of a further dose of paracetamol as documented by determination of serum enzyme activities (glutamic-oxaloacetic transaminase, (GOT), glutamic-pyruvic transaminase (GPT), sorbitol deh...


    pub_type: 杂志文章


    authors: Strubelt O,Siegers CP,Völpel M,Younes M

    更新日期:1979-02-01 00:00:00

  • Species specificity of 2-aryl carbapenem-induced immune-mediated hemolytic anemia in primates.

    abstract::L-695,256 is a novel 2-fluorenonyl carbapenem antibiotic with significant antimicrobial activity against strains of methicillin-resistant Staphylococci. This prototype compound was administered intravenously to rhesus monkeys (Macaca mulatta) at does of 50 or 200 mg/kg/day for 2 weeks to assess toxicity and found to i...


    pub_type: 杂志文章


    authors: Lankas GR,Coleman JB,Klein HJ,Bailly Y

    更新日期:1996-04-30 00:00:00

  • Interaction of air pollutants and pulmonary allergic responses in experimental animals.

    abstract::Asthma, which is primarily an allergic type of respiratory disease, has increased in the U.S. and Europe by 30% over the last decade. Air pollution may play a role in this rise, since during episodes of smog, hospital admissions due to asthma increase. Ambient air quality has generally improved since the Clean Air Act...


    pub_type: 杂志文章,评审


    authors: Gilmour MI

    更新日期:1995-12-28 00:00:00

  • Potential role for IL-5 and IL-6 in enhanced IgA secretion by Peyer's patch cells isolated from mice acutely exposed to vomitoxin.

    abstract::Dietary exposure to vomitoxin (VT) results in hyperelevated serum IgA and IgA nephropathy in mice. To assess the possible role of cytokines in this IgA dysregulation, the effects of a single oral exposure in B6C3F1 male mice to 0, 5 or 25 mg/kg BW VT on production of IgA and cytokines in Peyer's patch (PP) and spleen ...


    pub_type: 杂志文章


    authors: Yan D,Zhou HR,Brooks KH,Pestka JJ

    更新日期:1997-09-26 00:00:00

  • Carbon tetrachloride changes the activity of cytochrome P450 system in the liver of male rats: role of antioxidants.

    abstract::The cytochrome P-450 enzymes are responsible for the oxidation of xenobiotic chemicals including drugs, pesticides, and carcinogens. These enzymes include cytochrome P450, cytochrome b(5), arylhydrocarbon (benzo[a]pyrene) hydroxylase (AHH), NADPH-cytochrome C reductase and dimethylnitrosamine N-demethylase I (DMN-dI)....


    pub_type: 杂志文章


    authors: Sheweita SA,El-Gabar MA,Bastawy M

    更新日期:2001-12-14 00:00:00

  • V79 Chinese hamster cells genetically engineered for cytochrome P450 and their use in mutagenicity and metabolism studies.

    abstract::V79 Chinese hamster cells are being genetically engineered for stable expression of cytochromes P450 using a SV40 early promoter containing plasmid as the eukaryotic expression vector. V79 cells lack endogenous cytochrome P450 activity. Therefore, genetically engineered V79 cell lines are defined for the cytochrome P4...


    pub_type: 杂志文章,评审


    authors: Doehmer J

    更新日期:1993-10-05 00:00:00

  • Effects of lead microparticles on the growth characteristics of the BHK21 fibroblast cell line.

    abstract::BHK21 fibroblastic cells were exposed in vitro to lead microparticles produced by pyrolysis of the organic lead used as an antiknock agent in gasoline. Although non-cytolethal, 10 micrograms of lead/10(6) cells rapidly inhibited cell growth kinetics as well as [3H]thymidine and L-[3H]leucine incorporation by the BHK21...


    pub_type: 杂志文章


    authors: Dubreuil A,Hollande E,Bouley G,Boudene C

    更新日期:1979-08-01 00:00:00

  • Dietary zinc deficiency induced-changes in the activity of enzymes and the levels of free radicals, lipids and protein electrophoretic behavior in growing rats.

    abstract::Zinc (Zn) is an essential nutrient that is required in humans and animals for many physiological functions, including immune and antioxidant function, growth and reproduction. The present study was conducted to investigate the effects of adequate Zn level (38 mg/kg diet, as a control) and two low levels that create Zn...


    pub_type: 杂志文章


    authors: Yousef MI,El-Hendy HA,El-Demerdash FM,Elagamy EI

    更新日期:2002-06-14 00:00:00

  • Occupational health management and service for small-scale industries in Shanghai.

    abstract::Because occupational hazards of small-scale industries were severe and the occupational health management lagged behind in Shanghai, we selected some districts as experimental units to explore a model of community management and service for the occupational health in small-scale industries for controlling occupational...


    pub_type: 杂志文章


    authors: Wei L,Shi-Da W

    更新日期:2004-05-20 00:00:00

  • Reactive oxygen species accumulation contributes to gambogic acid-induced apoptosis in human hepatoma SMMC-7721 cells.

    abstract::It is reported that gambogic acid (GA), the main active compound of gamboge which is a dry resin extracted from Garcinia hanburyi tree, has potent antitumor activity both in vivo and in vitro. Activation of mitochondrial apoptotic pathway in cancer cells is one effective therapy for cancer treatment. In the present st...


    pub_type: 杂志文章


    authors: Nie F,Zhang X,Qi Q,Yang L,Yang Y,Liu W,Lu N,Wu Z,You Q,Guo Q

    更新日期:2009-06-16 00:00:00

  • Selective endothelin a (ETA) receptor antagonist (BQ-123) reduces both myocardial infarct size and oxidant injury.

    abstract:OBJECTIVE:Endothelins (ET) can be considered stress-responsive regulators working in paracrine and autocrine fashion. It has been suggested that elevated levels of ET may be responsible for the low coronary re-flow phenomena. Ischemia-reperfusion (I/R) was shown to stimulate ET release in rat heart; however, the mechan...


    pub_type: 杂志文章


    authors: Ozdemir R,Parlakpinar H,Polat A,Colak C,Ermis N,Acet A

    更新日期:2006-02-15 00:00:00

  • Fenvalerate inhibits the growth of primary cultured rat preantral ovarian follicles.

    abstract::Fenvalerate is a widely used synthetic pyrethroid insecticide and is reported to disrupt reproductive function in humans and animals. However, little is known about its influence on follicular development. In this study, rat preantral follicles were primary cultured to investigate the effects of fenvalerate on follicu...


    pub_type: 杂志文章


    authors: Fei J,Qu JH,Ding XL,Xue K,Lu CC,Chen JF,Song L,Xia YK,Wang SL,Wang XR

    更新日期:2010-01-12 00:00:00

  • The clinical toxicology of carbon monoxide.

    abstract::Carbon monoxide (CO) is a dangerous exogenous poison and an essential endogenous neurotransmitter. This gas when inhaled has an anaesthetic effect, which is poorly understood, but which may be fatal if compensatory mechanisms are exhausted, if cardiac oxygen (O(2)) needs exceed myocardial oxygenation and/or if apnoea ...


    pub_type: 杂志文章,评审


    authors: Gorman D,Drewry A,Huang YL,Sames C

    更新日期:2003-05-01 00:00:00

  • Toxicity of inorganic arsenic and its metabolites on haematopoietic progenitors "in vitro": comparison between species and sexes.

    abstract::Inorganic arsenic (iAs) and its metabolites are transferred to the foetus through the placental barrier and this exposure can compromise the normal development of the unborn. For this reason, we assessed the toxicity of sodium arsenite (iAs(III)) and its metabolites dimethylarsinic acid (DMA(V)), monomethylarsonic aci...


    pub_type: 杂志文章


    authors: Ferrario D,Croera C,Brustio R,Collotta A,Bowe G,Vahter M,Gribaldo L

    更新日期:2008-07-30 00:00:00

  • Hydrogen peroxide induces premature acrosome reaction in rat sperm and reduces their penetration of the zona pellucida.

    abstract::Recent studies have demonstrated that mammalian sperm are capable of generating reactive oxygen species (ROS) and that this activity is significantly accelerated in subfertile subjects. The observed decrease in penetration of zona-intact oocyte might be explained by chemical-induced ROS-related early onset of capacita...


    pub_type: 杂志文章


    authors: Hsu PC,Hsu CC,Guo YL

    更新日期:1999-11-29 00:00:00

  • Sex and strain differences of susceptibility to methylmercury toxicity in mice.

    abstract::Excretion and organ distribution of mercury and susceptibility to methylmercury (MeHg) toxicity were compared between strains and sexes after successive oral administration of MeHg chloride (5 mg/kg per day) using BALB/cA (C) and C57BL/6N (B6) mice. Every mouse died several days after initiation of toxic symptoms, and...


    pub_type: 杂志文章


    authors: Yasutake A,Hirayama K

    更新日期:1988-09-01 00:00:00

  • Selective pancreatic toxicity of palmitoylpentachlorophenol.

    abstract::Palmitoylpentachlorophenol (PPCP), which is a lipid conjugate of a xenobiotic compound, has been found in human fat. To study the toxicity associated with PPCP, rats were given 100 mg/kg PPCP and sacrificed at 4, 8 and 12 days. The target organ identified was the exocrine pancreas; no other major organs examined showe...


    pub_type: 杂志文章


    authors: Ansari GA,Kaphalia BS,Boor PJ

    更新日期:1987-10-12 00:00:00

  • Cytokine assays in human sera and tissues.

    abstract::The use of accurate and sensitive methods for the measurement of cytokines in body fluids is an absolute prerequisite for the proper use of these mediators in clinical practice. Many factors contribute to the complexity of cytokines quantitation: these molecules circulate at very low levels (e.g. pg/ml) under various ...


    pub_type: 杂志文章,评审


    authors: Bienvenu JA,Monneret G,Gutowski MC,Fabien N

    更新日期:1998-08-07 00:00:00

  • The interactions of cis-diamminedichloroplatinum with metallothionein and glutathione in rat liver and kidney.

    abstract::The involvement of metallothionein (MT) in the nephrotoxicity of cis-diamminedichloroplatinum (c-DDP) was investigated in rats using enzyme excretion and histology as indicators of renal damage. In addition, the effects of renal glutathione (GSH) depletion on the nephrotoxicity of c-DDP was assessed by organic anion t...


    pub_type: 杂志文章


    authors: Suzuki CA,Cherian MG

    更新日期:1990-11-01 00:00:00

  • Cytokine release does not improve the sensitivity and specificity of the direct popliteal lymph node assay.

    abstract::The popliteal lymph node assay (PLNA) is being considered as a tool to predict the potential of drugs for inducing systemic autoimmune and hypersensitivity reactions. Despite the use of different technical approaches and the evaluation of over 130 compounds, the sensitivity and specificity of the PLNA are still debata...


    pub_type: 杂志文章


    authors: Ravel G,Christ M,Horand F,Descotes J

    更新日期:2004-08-05 00:00:00

  • Ozone exposure promotes pyroptosis in rat lungs via the TLR2/4-NF-κB-NLRP3 signaling pathway.

    abstract:OBJECTIVE:Ozone has become a major air pollutant in recent years, which leading to a variety of lung diseases. This study aimed to explore the mechanisms of pyroptosis and related signaling pathways in ozone-induced lung injury. METHODS:We exposed 120 Wistar rats to ozone, 20 in each group (half male and half female)....


    pub_type: 杂志文章


    authors: Tian L,Yan J,Li K,Zhang W,Lin B,Lai W,Bian L,Liu H,Xi Z,Liu X

    更新日期:2020-12-28 00:00:00

  • Comparative carcinogenicity of two structurally similar phenylenediamine dyes (HC blue no. 1 and HC blue no. 2) in F344/N rats and B6C3F1 mice.

    abstract::Toxicology and carcinogenesis studies of 2 structurally-related p-phenylenediamines, HC Blue No. 1, and HC Blue No. 2 were conducted by administering each chemical in feed for 103 weeks to both sexes of Fischer 344/N rats and B6C3F1 (C57BL/6N x C3H/HEN) mice. Diets containing 0, 1500, or 3000 ppm HC Blue 1 were fed to...


    pub_type: 杂志文章


    authors: Kari FW,Mennear JH,Farnell D,Thompson RB,Huff JE

    更新日期:1989-06-01 00:00:00

  • [Nicotine metabolism in the pig (author's transl)].

    abstract::After i.v. injection of 0.33 mg nicotine/kg the elimination of unchanged nicotine as well as nicotine-N-oxide, cotinine, nornicotine and norcotinine was determined in the urine of pigs dependent on time. In female pigs 16.3% and in male pigs 25.9% of the applied nicotine were recovered in form of the mentioned compoun...


    pub_type: 杂志文章


    authors: Harke HP,Frahm B

    更新日期:1976-06-01 00:00:00

  • Nitrogen mustard (Chlorambucil) has a negative influence on early vascular development.

    abstract::The sulphur and nitrogen mustards are strong alkylating agents, which induces inflammations of the skin including blistering right up to ulcerations. Depending on the severity, the wounds may need weeks to heal. In the past it was shown that sulphur mustard has a destructive effect on endothelial precursor cells, whic...


    pub_type: 杂志文章


    authors: Schmidt A,Bölck B,Jedig M,Steinritz D,Balszuweit F,Kehe K,Bloch W

    更新日期:2009-09-01 00:00:00

  • Muscle force and acetylcholinesterase activity in mouse hemidiaphragms exposed to paraoxon and treated by oximes in vitro.

    abstract::The therapy of organophosphorus compound (OP) poisoning is still a challenge to clinical toxicologists. To alleviate peripheral respiratory failure oximes, e.g. obidoxime and pralidoxime, are used to reactivate inhibited acetylcholinesterase (AChE) with the intention to restore the disturbed neuromuscular function. In...


    pub_type: 杂志文章


    authors: Thiermann H,Eyer P,Worek F

    更新日期:2010-06-04 00:00:00

  • Evaluation of nitrofurantoin on the two stages of urinary bladder carcinogenesis in the rat.

    abstract::The possibility that nitrofurantoin is a complete carcinogen or is an initiator or promoter of urinary bladder carcinogenesis was evaluated in male weanling F344 rats. No increase in tumor incidence was observed in rats fed nitrofurantoin at a level of 0.187% of the diet for 2 years compared to a control group. Also, ...


    pub_type: 杂志文章


    authors: Hasegawa R,Murasaki G,St John MK,Zenser TV,Cohen SM

    更新日期:1990-06-01 00:00:00