Functional contributions of alpha5 subunit to neuronal acetylcholine receptor channels.


:Ligand-gated ion channels are multi-subunit complexes where each subunit-type is encoded by several related genes. Heterologous expression of any one of the neuronal nicotinic acetylcholine receptors (nAChR) alpha-type subunits, either alone or with any beta-type subunit, typically yields functional nAChR channels. A striking exception is the nAChR alpha5 subunit: although apparently complexed with beta2 and beta4 nAChR subunits in neurons, and expressed in a subset of neurons within the central and peripheral nervous systems, heterologous expression of alpha5, either alone or with any beta-type subunit has failed to yield functional channels. We demonstrate here that alpha5 does participate in nAChRs expressed in hetrologous systems and in primary neurons, and further that alpha5 contributes to the lining of functionally unique nAChR channels, but only if coexpressed with both another alpha- and beta-type subunit. Furthermore, channels containing the alpha5 subunit are potently activated and desensitized by nanomolar concentrations of nicotine.






Ramirez-Latorre J,Yu CR,Qu X,Perin F,Karlin A,Role L




Has Abstract


1996-03-28 00:00:00














  • Release of chromatin protein HMGB1 by necrotic cells triggers inflammation.

    abstract::High mobility group 1 (HMGB1) protein is both a nuclear factor and a secreted protein. In the cell nucleus it acts as an architectural chromatin-binding factor that bends DNA and promotes protein assembly on specific DNA targets. Outside the cell, it binds with high affinity to RAGE (the receptor for advanced glycatio...


    pub_type: 杂志文章


    authors: Scaffidi P,Misteli T,Bianchi ME

    更新日期:2002-07-11 00:00:00

  • Evidence against dust-mediated control of glacial-interglacial changes in atmospheric CO2.

    abstract::The low concentration of atmospheric CO2 inferred to have been present during glacial periods is thought to have been partly caused by an increased supply of iron-bearing dust to the ocean surface. This is supported by a recent model that attributes half of the CO2 reduction during past glacial stages to iron-stimulat...


    pub_type: 历史文章,杂志文章


    authors: Maher BA,Dennis PF

    更新日期:2001-05-10 00:00:00

  • Strong emission of methyl chloride from tropical plants.

    abstract::Methyl chloride is the largest natural source of ozone-depleting chlorine compounds, and accounts for about 15 per cent of the present atmospheric chlorine content. This contribution was likely to have been relatively greater in pre-industrial times, when additional anthropogenic sources-such as chlorofluorocarbons-we...


    pub_type: 杂志文章


    authors: Yokouchi Y,Ikeda M,Inuzuka Y,Yukawa T

    更新日期:2002-03-14 00:00:00

  • Synthesis of growth hormone by bacteria.

    abstract::A hybrid gene was constructed between the beta-lactamase gene of plasmid pBR322 and the cloned coding sequence for rat growth hormone. This gene is expressed in bacteria and growth hormone sequences are detectable by immunological methods. ...


    pub_type: 杂志文章


    authors: Seeburg PH,Shine J,Martial JA,Ivarie RD,Morris JA,Ullrich A,Baxter JD,Goodman HM

    更新日期:1978-12-21 00:00:00

  • Somatic hypermutation of an immunoglobulin transgene in kappa transgenic mice.

    abstract::Initial studies of somatically acquired mutations in immunoglobulin V regions from hybridomas and myelomas that are not derived from joining aberrations, suggested a controlled and specific hypermutation process, because spontaneous mutation rates observed for other genes are extremely low. Some evidence for the idea ...


    pub_type: 杂志文章


    authors: O'Brien RL,Brinster RL,Storb U

    更新日期:1987-03-26 00:00:00

  • An eIF-4A-like protein is a suppressor of an Escherichia coli mutant defective in 50S ribosomal subunit assembly.

    abstract::The assembly of ribosomes in bacterial cells is a complex process that remains poorly characterized. The in vitro assembly of active ribosomal subunits from purified RNA and protein components indicates that all of the information for proper assembly resides in the primary sequences of these macromolecules. On the oth...


    pub_type: 杂志文章


    authors: Nishi K,Morel-Deville F,Hershey JW,Leighton T,Schnier J

    更新日期:1988-12-01 00:00:00

  • Computer logic meets cell biology: how cell science is getting an upgrade.

    abstract:: ...


    pub_type: 杂志文章


    authors: Savage N

    更新日期:2018-12-01 00:00:00

  • Migratory neural crest-like cells form body pigmentation in a urochordate embryo.

    abstract::The neural crest, a source of many different cell types in vertebrate embryos, has not been identified in other chordates. Current opinion therefore holds that neural crest cells were a vertebrate innovation. Here we describe a migratory cell population resembling neural crest cells in the ascidian urochordate Ecteina...


    pub_type: 杂志文章


    authors: Jeffery WR,Strickler AG,Yamamoto Y

    更新日期:2004-10-07 00:00:00

  • Synaptotagmin 7 confers frequency invariance onto specialized depressing synapses.

    abstract::At most synapses in the brain, short-term plasticity dynamically modulates synaptic strength. Rapid frequency-dependent changes in synaptic strength have key roles in sensory adaptation, gain control and many other neural computations. However, some auditory, vestibular and cerebellar synapses maintain constant streng...


    pub_type: 杂志文章


    authors: Turecek J,Jackman SL,Regehr WG

    更新日期:2017-11-23 00:00:00

  • A central role for TFIID in the pluripotent transcription circuitry.

    abstract::Embryonic stem (ES) cells are pluripotent and characterized by open chromatin and high transcription levels, achieved through auto-regulatory and feed-forward transcription factor loops. ES-cell identity is maintained by a core of factors including Oct4 (also known as Pou5f1), Sox2, Klf4, c-Myc (OSKM) and Nanog, and f...


    pub_type: 杂志文章


    authors: Pijnappel WW,Esch D,Baltissen MP,Wu G,Mischerikow N,Bergsma AJ,van der Wal E,Han DW,Bruch Hv,Moritz S,Lijnzaad P,Altelaar AF,Sameith K,Zaehres H,Heck AJ,Holstege FC,Schöler HR,Timmers HT

    更新日期:2013-03-28 00:00:00

  • Contour from motion processing occurs in primary visual cortex.

    abstract::Relative motion is one of the most salient cues for segmentation of a visual scene into separate objects. This is illustrated by the vivid contours that are perceived when random dot patterns move in different directions. Once motion is halted in such displays the segmentation contours disappear. This makes random dot...


    pub_type: 杂志文章


    authors: Lamme VA,van Dijk BW,Spekreijse H

    更新日期:1993-06-10 00:00:00

  • Dynamics of the silicon (111) surface phase transition

    abstract::The manner in which phase transformations occur in solids determines important structural and physical properties of many materials. The main problem in characterizing the kinetic processes that occur during phase transformations is the difficulty of observing directly, in real time, the growth of one phase at the exp...


    pub_type: 杂志文章


    authors: Hannon JB,Hibino H,Bartelt NC,Swartzentruber BS,Ogino T,Kellogg GL

    更新日期:2000-06-01 00:00:00

  • Enabling personalized cancer medicine through analysis of gene-expression patterns.

    abstract::Therapies for patients with cancer have changed gradually over the past decade, moving away from the administration of broadly acting cytotoxic drugs towards the use of more-specific therapies that are targeted to each tumour. To facilitate this shift, tests need to be developed to identify those individuals who requi...


    pub_type: 杂志文章,评审


    authors: van't Veer LJ,Bernards R

    更新日期:2008-04-03 00:00:00

  • LRG1 promotes angiogenesis by modulating endothelial TGF-β signalling.

    abstract::Aberrant neovascularization contributes to diseases such as cancer, blindness and atherosclerosis, and is the consequence of inappropriate angiogenic signalling. Although many regulators of pathogenic angiogenesis have been identified, our understanding of this process is incomplete. Here we explore the transcriptome ...


    pub_type: 杂志文章


    authors: Wang X,Abraham S,McKenzie JAG,Jeffs N,Swire M,Tripathi VB,Luhmann UFO,Lange CAK,Zhai Z,Arthur HM,Bainbridge J,Moss SE,Greenwood J

    更新日期:2013-07-18 00:00:00

  • Phylogenetic evidence for Sino-Tibetan origin in northern China in the Late Neolithic.

    abstract::The study of language origin and divergence is important for understanding the history of human populations and their cultures. The Sino-Tibetan language family is the second largest in the world after Indo-European, and there is a long-running debate about its phylogeny and the time depth of its original divergence1....


    pub_type: 历史文章,杂志文章


    authors: Zhang M,Yan S,Pan W,Jin L

    更新日期:2019-05-01 00:00:00

  • Horsetails and ferns are a monophyletic group and the closest living relatives to seed plants.

    abstract::Most of the 470-million-year history of plants on land belongs to bryophytes, pteridophytes and gymnosperms, which eventually yielded to the ecological dominance by angiosperms 90 Myr ago. Our knowledge of angiosperm phylogeny, particularly the branching order of the earliest lineages, has recently been increased by t...


    pub_type: 杂志文章


    authors: Pryer KM,Schneider H,Smith AR,Cranfill R,Wolf PG,Hunt JS,Sipes SD

    更新日期:2001-02-01 00:00:00

  • Epsin is an EH-domain-binding protein implicated in clathrin-mediated endocytosis.

    abstract::During endocytosis, clathrin and the clathrin adaptor protein AP-2, assisted by a variety of accessory factors, help to generate an invaginated bud at the cell membrane. One of these factors is Eps15, a clathrin-coat-associated protein that binds the alpha-adaptin subunit of AP-2. Here we investigate the function of E...


    pub_type: 杂志文章


    authors: Chen H,Fre S,Slepnev VI,Capua MR,Takei K,Butler MH,Di Fiore PP,De Camilli P

    更新日期:1998-08-20 00:00:00

  • Cloning and DNA sequence of double-stranded copies of haemagglutinin genes from H2 and H3 strains elucidates antigenic shift and drift in human influenza virus.

    abstract::Double-stranded DNA copies of the RNA gene coding for the haemagglutinin glycoproteins from human H2 and H3 pandemic strains of influenza virus have been cloned. DNA sequence analysis provides the first reported complete nucleotide sequence of an H2 haemagglutinin gene and a partial sequence (45%) of the H3 gene. The ...


    pub_type: 杂志文章


    authors: Gething MJ,Bye J,Skehel J,Waterfield M

    更新日期:1980-09-25 00:00:00

  • Direct detection of a break in the teraelectronvolt cosmic-ray spectrum of electrons and positrons.

    abstract::High-energy cosmic-ray electrons and positrons (CREs), which lose energy quickly during their propagation, provide a probe of Galactic high-energy processes and may enable the observation of phenomena such as dark-matter particle annihilation or decay. The CRE spectrum has been measured directly up to approximately 2 ...


    pub_type: 杂志文章


    authors: DAMPE Collaboration.

    更新日期:2017-12-07 00:00:00

  • Major histocompatibility complex class I molecules internalized via coated pits in T lymphocytes.

    abstract::Endocytosis of the major histocompatibility complex (MHC)-encoded class I and class II molecules has been the subject of recent investigations. Class I molecules, which are key elements in T cell-mediated cytotoxicity, are differentially endocytosed by different cell types. Fibroblasts internalize their class I molecu...


    pub_type: 杂志文章


    authors: Machy P,Truneh A,Gennaro D,Hoffstein S

    更新日期:1987-08-20 00:00:00

  • Crystal structure of the bacterial cell-division protein FtsZ.

    abstract::Bacterial cell division ends with septation, the constriction of the cell wall and cell membranes that leads to the formation of two daughter cells. During septation, FtsZ, a protein of relative molecular mass 40,000 which is ubiquitous in eubacteria and is also found in archaea and chloroplasts, localizes early at th...


    pub_type: 杂志文章


    authors: Löwe J,Amos LA

    更新日期:1998-01-08 00:00:00

  • Olfactory dysfunction in humans with deficient guanine nucleotide-binding protein.

    abstract::The guanine nucleotide-binding stimulatory protein (Gs) couples hormone-receptor interaction to the activation of adenylate cyclase and the generation of cyclic AMP. Studies using frog neuroepithelium indicate that the sense of smell is mediated by a Gs-adenylate cyclase system, and this prompted us to test olfaction ...


    pub_type: 杂志文章


    authors: Weinstock RS,Wright HN,Spiegel AM,Levine MA,Moses AM

    更新日期:1986-08-14 00:00:00

  • Guard cell abscisic acid signalling and engineering drought hardiness in plants.

    abstract::Guard cells are located in the epidermis of plant leaves, and in pairs surround stomatal pores. These control both the influx of CO2 as a raw material for photosynthesis and water loss from plants through transpiration to the atmosphere. Guard cells have become a highly developed system for dissecting early signal tra...


    pub_type: 杂志文章,评审


    authors: Schroeder JI,Kwak JM,Allen GJ

    更新日期:2001-03-15 00:00:00

  • Interplay of electron-lattice interactions and superconductivity in superconductivity in Bi2Sr2CaCu2O8+delta.

    abstract::Formation of electron pairs is essential to superconductivity. For conventional superconductors, tunnelling spectroscopy has established that pairing is mediated by bosonic modes (phonons); a peak in the second derivative of tunnel current d2I/dV2 corresponds to each phonon mode. For high-transition-temperature (high-...


    pub_type: 杂志文章


    authors: Lee J,Fujita K,McElroy K,Slezak JA,Wang M,Aiura Y,Bando H,Ishikado M,Masui T,Zhu JX,Balatsky AV,Eisaki H,Uchida S,Davis JC

    更新日期:2006-08-03 00:00:00

  • Human cyclin A is adenovirus E1A-associated protein p60 and behaves differently from cyclin B.

    abstract::CYCLINS are proteins synthesized during each cell cycle and abruptly destroyed in each mitosis. Cyclins have been implicated in the induction of mitosis and are associated with the serine-threonine protein kinase p34cdc2 as components of mitosis promoting factor (MPF). On the basis of conserved sequence motifs cyclins...


    pub_type: 杂志文章


    authors: Pines J,Hunter T

    更新日期:1990-08-23 00:00:00

  • Molecular basis of seasonal time measurement in Arabidopsis.

    abstract::Several organisms have evolved the ability to measure daylength, or photoperiod, allowing them to adjust their development in anticipation of annual seasonal changes. Daylength measurement requires the integration of temporal information, provided by the circadian system, with light/dark discrimination, initiated by s...


    pub_type: 杂志文章


    authors: Yanovsky MJ,Kay SA

    更新日期:2002-09-19 00:00:00

  • Comprehensive polymorphism survey elucidates population structure of Saccharomyces cerevisiae.

    abstract::Comprehensive identification of polymorphisms among individuals within a species is essential both for studying the genetic basis of phenotypic differences and for elucidating the evolutionary history of the species. Large-scale polymorphism surveys have recently been reported for human, mouse and Arabidopsis thaliana...


    pub_type: 杂志文章


    authors: Schacherer J,Shapiro JA,Ruderfer DM,Kruglyak L

    更新日期:2009-03-19 00:00:00

  • Cobalt-56 γ-ray emission lines from the type Ia supernova 2014J.

    abstract::A type Ia supernova is thought to be a thermonuclear explosion of either a single carbon-oxygen white dwarf or a pair of merging white dwarfs. The explosion fuses a large amount of radioactive (56)Ni (refs 1-3). After the explosion, the decay chain from (56)Ni to (56)Co to (56)Fe generates γ-ray photons, which are rep...


    pub_type: 杂志文章


    authors: Churazov E,Sunyaev R,Isern J,Knödlseder J,Jean P,Lebrun F,Chugai N,Grebenev S,Bravo E,Sazonov S,Renaud M

    更新日期:2014-08-28 00:00:00

  • Evolution of distinct developmental functions of three Drosophila genes by acquisition of different cis-regulatory regions.

    abstract::It is generally accepted that the specific function of a gene depends on its coding sequence. The three paired-box and homeobox genes paired (prd), gooseberry (gsb) and gooseberry neuro (gsbn) have distinct developmental functions in Drosophila embryogenesis. During the syncytial blastoderm stage, the pair-rule gene p...


    pub_type: 杂志文章


    authors: Li X,Noll M

    更新日期:1994-01-06 00:00:00

  • Mechanosensitivity of mammalian auditory hair cells in vitro.

    abstract::Intracellular responses recorded in vitro from the cochleas of anaesthetized mammals have shown that the mechanoreceptive inner and outer hair cells are sharply tuned, accounting for many of the properties of the afferent fibres in the auditory nerve. However, in vivo it has not been possible to measure directly the e...


    pub_type: 杂志文章


    authors: Russell IJ,Richardson GP,Cody AR

    更新日期:1986-05-29 00:00:00