Functional contributions of alpha5 subunit to neuronal acetylcholine receptor channels.


:Ligand-gated ion channels are multi-subunit complexes where each subunit-type is encoded by several related genes. Heterologous expression of any one of the neuronal nicotinic acetylcholine receptors (nAChR) alpha-type subunits, either alone or with any beta-type subunit, typically yields functional nAChR channels. A striking exception is the nAChR alpha5 subunit: although apparently complexed with beta2 and beta4 nAChR subunits in neurons, and expressed in a subset of neurons within the central and peripheral nervous systems, heterologous expression of alpha5, either alone or with any beta-type subunit has failed to yield functional channels. We demonstrate here that alpha5 does participate in nAChRs expressed in hetrologous systems and in primary neurons, and further that alpha5 contributes to the lining of functionally unique nAChR channels, but only if coexpressed with both another alpha- and beta-type subunit. Furthermore, channels containing the alpha5 subunit are potently activated and desensitized by nanomolar concentrations of nicotine.






Ramirez-Latorre J,Yu CR,Qu X,Perin F,Karlin A,Role L




Has Abstract


1996-03-28 00:00:00














  • Premotor commands encode monocular eye movements.

    abstract::Binocular coordination of eye movements is essential for stereopsis (depth perception) and to prevent double vision. More than a century ago, Hering and Helmholtz debated the neural basis of binocular coordination. Helmholtz believed that each eye is controlled independently and that binocular coordination is learned....


    pub_type: 杂志文章


    authors: Zhou W,King WM

    更新日期:1998-06-18 00:00:00

  • SHARPIN is a component of the NF-κB-activating linear ubiquitin chain assembly complex.

    abstract::Cpdm (chronic proliferative dermatitis) mice develop chronic dermatitis and an immunodeficiency with increased serum IgM, symptoms that resemble those of patients with X-linked hyper-IgM syndrome and hypohydrotic ectodermal dysplasia (XHM-ED), which is caused by mutations in NEMO (NF-κB essential modulator; also known...


    pub_type: 杂志文章


    authors: Tokunaga F,Nakagawa T,Nakahara M,Saeki Y,Taniguchi M,Sakata S,Tanaka K,Nakano H,Iwai K

    更新日期:2011-03-31 00:00:00

  • Duplications of a mutated simian virus 40 enhancer restore its activity.

    abstract::Enhancers are cis-acting control elements which can stimulate at a distance the activity of a variety of eukaryotic promoters. First identified as a repeated 72 base pair (bp) sequence upstream of the simian virus 40 (SV40) early gene promoter, enhancers have since been shown to be associated with numerous other viral...


    pub_type: 杂志文章


    authors: Herr W,Gluzman Y

    更新日期:1985-02-21 00:00:00

  • Five innovative ways to use 3D printing in the laboratory.

    abstract:: ...


    pub_type: 新闻


    authors: Silver A

    更新日期:2019-01-01 00:00:00

  • The dating of shallow faults in the Earth's crust.

    abstract::Direct dating of ductile shear zones and calculation of uplift/exhumation rates can be done using various radiometric dating techniques. But radiometric dating of shallow crustal faulting, which occurs in the crust's brittle regime, has remained difficult because the low temperatures typical of shallow crusted faults ...


    pub_type: 杂志文章


    authors: van der Pluijm BA,Hall CM,Vrolijk PJ,Pevear DR,Covey MC

    更新日期:2001-07-12 00:00:00

  • Extreme damping in composite materials with negative-stiffness inclusions.

    abstract::When a force deforms an elastic object, practical experience suggests that the resulting displacement will be in the same direction as the force. This property is known as positive stiffness. Less familiar is the concept of negative stiffness, where the deforming force and the resulting displacement are in opposite di...


    pub_type: 杂志文章


    authors: Lakes RS,Lee T,Bersie A,Wang YC

    更新日期:2001-03-29 00:00:00

  • Human-level control through deep reinforcement learning.

    abstract::The theory of reinforcement learning provides a normative account, deeply rooted in psychological and neuroscientific perspectives on animal behaviour, of how agents may optimize their control of an environment. To use reinforcement learning successfully in situations approaching real-world complexity, however, agents...


    pub_type: 杂志文章


    authors: Mnih V,Kavukcuoglu K,Silver D,Rusu AA,Veness J,Bellemare MG,Graves A,Riedmiller M,Fidjeland AK,Ostrovski G,Petersen S,Beattie C,Sadik A,Antonoglou I,King H,Kumaran D,Wierstra D,Legg S,Hassabis D

    更新日期:2015-02-26 00:00:00

  • Structural basis of glutamate recognition by a dimeric metabotropic glutamate receptor.

    abstract::The metabotropic glutamate receptors (mGluRs) are key receptors in the modulation of excitatory synaptic transmission in the central nervous system. Here we have determined three different crystal structures of the extracellular ligand-binding region of mGluR1--in a complex with glutamate and in two unliganded forms. ...


    pub_type: 杂志文章


    authors: Kunishima N,Shimada Y,Tsuji Y,Sato T,Yamamoto M,Kumasaka T,Nakanishi S,Jingami H,Morikawa K

    更新日期:2000-10-26 00:00:00

  • India: a turbulent tale of rivers, floods and monsoons.

    abstract:: ...


    pub_type: 新闻


    authors: Ball P

    更新日期:2018-12-01 00:00:00

  • The avian Z-linked gene DMRT1 is required for male sex determination in the chicken.

    abstract::Sex in birds is chromosomally based, as in mammals, but the sex chromosomes are different and the mechanism of avian sex determination has been a long-standing mystery. In the chicken and all other birds, the homogametic sex is male (ZZ) and the heterogametic sex is female (ZW). Two hypotheses have been proposed for t...


    pub_type: 杂志文章


    authors: Smith CA,Roeszler KN,Ohnesorg T,Cummins DM,Farlie PG,Doran TJ,Sinclair AH

    更新日期:2009-09-10 00:00:00

  • Ion sieving in graphene oxide membranes via cationic control of interlayer spacing.

    abstract::Graphene oxide membranes-partially oxidized, stacked sheets of graphene-can provide ultrathin, high-flux and energy-efficient membranes for precise ionic and molecular sieving in aqueous solution. These materials have shown potential in a variety of applications, including water desalination and purification, gas and ...


    pub_type: 杂志文章


    authors: Chen L,Shi G,Shen J,Peng B,Zhang B,Wang Y,Bian F,Wang J,Li D,Qian Z,Xu G,Liu G,Zeng J,Zhang L,Yang Y,Zhou G,Wu M,Jin W,Li J,Fang H

    更新日期:2017-10-19 00:00:00

  • Sleep in Drosophila is regulated by adult mushroom bodies.

    abstract::Sleep is one of the few major whole-organ phenomena for which no function and no underlying mechanism have been conclusively demonstrated. Sleep could result from global changes in the brain during wakefulness or it could be regulated by specific loci that recruit the rest of the brain into the electrical and metaboli...


    pub_type: 杂志文章


    authors: Joiner WJ,Crocker A,White BH,Sehgal A

    更新日期:2006-06-08 00:00:00

  • Gut intraepithelial T cells calibrate metabolism and accelerate cardiovascular disease.

    abstract::The biochemical response to food intake must be precisely regulated. Because ingested sugars and fats can feed into many anabolic and catabolic pathways1, how our bodies handle nutrients depends on strategically positioned metabolic sensors that link the intrinsic nutritional value of a meal with intermediary metaboli...


    pub_type: 杂志文章


    authors: He S,Kahles F,Rattik S,Nairz M,McAlpine CS,Anzai A,Selgrade D,Fenn AM,Chan CT,Mindur JE,Valet C,Poller WC,Halle L,Rotllan N,Iwamoto Y,Wojtkiewicz GR,Weissleder R,Libby P,Fernández-Hernando C,Drucker DJ,Nahrendorf

    更新日期:2019-02-01 00:00:00

  • Sensitization of silicon by singlet exciton fission in tetracene.

    abstract::Silicon dominates contemporary solar cell technologies1. But when absorbing photons, silicon (like other semiconductors) wastes energy in excess of its bandgap2. Reducing these thermalization losses and enabling better sensitivity to light is possible by sensitizing the silicon solar cell using singlet exciton fission...


    pub_type: 杂志文章


    authors: Einzinger M,Wu T,Kompalla JF,Smith HL,Perkinson CF,Nienhaus L,Wieghold S,Congreve DN,Kahn A,Bawendi MG,Baldo MA

    更新日期:2019-07-01 00:00:00

  • An N-terminal peptide from p60src can direct myristylation and plasma membrane localization when fused to heterologous proteins.

    abstract::The src gene product, p60src, of Rous sarcoma virus (RSV) is a tyrosine-specific protein kinase which is associated with the plasma membrane of infected cells. Myristic acid is bound in an amide linkage to glycine 2 of p60src. Of the N-terminal 30 kilodaltons of p60src, only amino acids 1-14 are required for myristyla...


    pub_type: 杂志文章


    authors: Pellman D,Garber EA,Cross FR,Hanafusa H

    更新日期:1985-03-28 00:00:00

  • Programmed population control by cell-cell communication and regulated killing.

    abstract::De novo engineering of gene circuits inside cells is extremely difficult, and efforts to realize predictable and robust performance must deal with noise in gene expression and variation in phenotypes between cells. Here we demonstrate that by coupling gene expression to cell survival and death using cell-cell communic...


    pub_type: 杂志文章


    authors: You L,Cox RS 3rd,Weiss R,Arnold FH

    更新日期:2004-04-22 00:00:00

  • Characterization of the 1S-2S transition in antihydrogen.

    abstract::In 1928, Dirac published an equation 1 that combined quantum mechanics and special relativity. Negative-energy solutions to this equation, rather than being unphysical as initially thought, represented a class of hitherto unobserved and unimagined particles-antimatter. The existence of particles of antimatter was conf...


    pub_type: 杂志文章


    authors: Ahmadi M,Alves BXR,Baker CJ,Bertsche W,Capra A,Carruth C,Cesar CL,Charlton M,Cohen S,Collister R,Eriksson S,Evans A,Evetts N,Fajans J,Friesen T,Fujiwara MC,Gill DR,Hangst JS,Hardy WN,Hayden ME,Isaac CA,Johnson M

    更新日期:2018-05-01 00:00:00

  • The mosaic that is our genome.

    abstract::The discovery of the basis of genetic variation has opened inroads to understanding our history as a species. It has revealed the remarkable genetic similarity we share with other individuals as well as with our closest primate relatives. To understand what make us unique, both as individuals and as a species, we need...


    pub_type: 杂志文章,评审


    authors: Pääbo S

    更新日期:2003-01-23 00:00:00

  • El Niño-Southern Oscillation complexity.

    abstract::El Niño events are characterized by surface warming of the tropical Pacific Ocean and weakening of equatorial trade winds that occur every few years. Such conditions are accompanied by changes in atmospheric and oceanic circulation, affecting global climate, marine and terrestrial ecosystems, fisheries and human activ...


    pub_type: 杂志文章,评审


    authors: Timmermann A,An SI,Kug JS,Jin FF,Cai W,Capotondi A,Cobb KM,Lengaigne M,McPhaden MJ,Stuecker MF,Stein K,Wittenberg AT,Yun KS,Bayr T,Chen HC,Chikamoto Y,Dewitte B,Dommenget D,Grothe P,Guilyardi E,Ham YG,Hayashi M

    更新日期:2018-07-01 00:00:00

  • Diversion of aspartate in ASS1-deficient tumours fosters de novo pyrimidine synthesis.

    abstract::Cancer cells hijack and remodel existing metabolic pathways for their benefit. Argininosuccinate synthase (ASS1) is a urea cycle enzyme that is essential in the conversion of nitrogen from ammonia and aspartate to urea. A decrease in nitrogen flux through ASS1 in the liver causes the urea cycle disorder citrullinaemia...


    pub_type: 杂志文章


    authors: Rabinovich S,Adler L,Yizhak K,Sarver A,Silberman A,Agron S,Stettner N,Sun Q,Brandis A,Helbling D,Korman S,Itzkovitz S,Dimmock D,Ulitsky I,Nagamani SC,Ruppin E,Erez A

    更新日期:2015-11-19 00:00:00

  • First-passage times in complex scale-invariant media.

    abstract::How long does it take a random walker to reach a given target point? This quantity, known as a first-passage time (FPT), has led to a growing number of theoretical investigations over the past decade. The importance of FPTs originates from the crucial role played by first encounter properties in various real situation...


    pub_type: 杂志文章


    authors: Condamin S,Bénichou O,Tejedor V,Voituriez R,Klafter J

    更新日期:2007-11-01 00:00:00

  • Author Correction: Universal resilience patterns in complex networks.

    abstract::In this Letter, in Fig. 3c and f the Saccharomyces cerevisiae and Escherichia coli networks were subject to both weight loss and node deletion, a combination of two types of perturbation, as opposed to weight loss only (as the labelling incorrectly indicated). The collapse in Fig. 3h was also obtained from this combin...


    pub_type: 已发布勘误


    authors: Gao J,Barzel B,Barabási AL

    更新日期:2019-04-01 00:00:00

  • Identification of a distinct synaptic glutamate receptor on horizontal cells in mudpuppy retina.

    abstract::The separation of ON and OFF channels and the development of an antagonistic surround occur at the first synapse in the vertebrate retina. This functional differentiation is mediated by the action of the photoreceptor neurotransmitter on the ON bipolar, OFF bipolar and horizontal cells, respectively. Glutamate mimics ...


    pub_type: 杂志文章


    authors: Slaughter MM,Miller RF

    更新日期:1985-03-07 00:00:00

  • Two growth factor signalling pathways in fibroblasts distinguished by pertussis toxin.

    abstract::The primary action of a family of mitogens including bombesin, bradykinin, vasopressin and alpha-thrombin is to activate the hydrolysis of polyphosphoinositides. Hydrolysis of phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2) by phospholipase C is mediated through coupling of surface receptors to a GTP-binding pro...


    pub_type: 杂志文章


    authors: Chambard JC,Paris S,L'Allemain G,Pouysségur J

    更新日期:1987-04-23 00:00:00

  • Crystal structure of a yeast TFIIA/TBP/DNA complex.

    abstract::The X-ray crystal structure of the transcription factor IIA (TFIIA) in complex with the TATA-box-binding protein (TBP) and TATA-element DNA is presented at 2.5 A resolution. TFIIA is composed of a beta-barrel and a four-helix bundle motif that together have a boot-like appearance. The beta-barrel extends the TBP beta-...


    pub_type: 杂志文章


    authors: Tan S,Hunziker Y,Sargent DF,Richmond TJ

    更新日期:1996-05-09 00:00:00

  • Phosphate oxygen isotopic evidence for a temperate and biologically active Archaean ocean.

    abstract::Oxygen and silicon isotope compositions of cherts and studies of protein evolution have been interpreted to reflect ocean temperatures of 55-85 degrees C during the early Palaeoarchaean era ( approximately 3.5 billion years ago). A recent study combining oxygen and hydrogen isotope compositions of cherts, however, mak...


    pub_type: 历史文章,杂志文章


    authors: Blake RE,Chang SJ,Lepland A

    更新日期:2010-04-15 00:00:00

  • The reaction cycle of GroEL and GroES in chaperonin-assisted protein folding.

    abstract::The reaction mechanism of protein folding by the chaperonin GroEL and its regulator GroES has been defined. GroES and substrate protein counteract each other's effects on GroEL: whereas GroES stabilizes GroEL in the ADP-bound state, binding of unfolded polypeptide within the cavity of the GroEL cylinder triggers ADP a...


    pub_type: 杂志文章


    authors: Martin J,Mayhew M,Langer T,Hartl FU

    更新日期:1993-11-18 00:00:00

  • Non-equivalence of Wnt and R-spondin ligands during Lgr5+ intestinal stem-cell self-renewal.

    abstract::The canonical Wnt/β-catenin signalling pathway governs diverse developmental, homeostatic and pathological processes. Palmitoylated Wnt ligands engage cell-surface frizzled (FZD) receptors and LRP5 and LRP6 co-receptors, enabling β-catenin nuclear translocation and TCF/LEF-dependent gene transactivation. Mutations in ...


    pub_type: 杂志文章


    authors: Yan KS,Janda CY,Chang J,Zheng GXY,Larkin KA,Luca VC,Chia LA,Mah AT,Han A,Terry JM,Ootani A,Roelf K,Lee M,Yuan J,Li X,Bolen CR,Wilhelmy J,Davies PS,Ueno H,von Furstenberg RJ,Belgrader P,Ziraldo SB,Ordonez H,H

    更新日期:2017-05-11 00:00:00

  • Precise dating of Dansgaard-Oeschger climate oscillations in western Europe from stalagmite data.

    abstract::The signature of Dansgaard-Oeschger events--millennial-scale abrupt climate oscillations during the last glacial period--is well established in ice cores and marine records. But the effects of such events in continental settings are not as clear, and their absolute chronology is uncertain beyond the limit of (14)C dat...


    pub_type: 杂志文章


    authors: Genty D,Blamart D,Ouahdi R,Gilmour M,Baker A,Jouzel J,Van-Exter S

    更新日期:2003-02-20 00:00:00

  • Synaptotagmin 7 confers frequency invariance onto specialized depressing synapses.

    abstract::At most synapses in the brain, short-term plasticity dynamically modulates synaptic strength. Rapid frequency-dependent changes in synaptic strength have key roles in sensory adaptation, gain control and many other neural computations. However, some auditory, vestibular and cerebellar synapses maintain constant streng...


    pub_type: 杂志文章


    authors: Turecek J,Jackman SL,Regehr WG

    更新日期:2017-11-23 00:00:00