Abstract:
:Several genes encoding subunits of the neuronal nicotinic acetylcholine receptors have been cloned and regulatory elements involved in the transcription of the alpha 2 and alpha 7-subunit genes have been described. Yet, the detailed mechanisms governing the neuron-specific transcription and the spatio-temporal expression pattern of these genes remain largely uninvestigated. The beta 2-subunit is the most widely expressed neuronal nicotinic receptor subunit in the nervous system. We have studied the structural and regulatory properties of the 5' sequence of this gene. A fragment of 1163 bp of upstream sequence is sufficient to drive the cell-specific transcription of a reporter gene in both transient transfection assays and in transgenic mice. Deletion analysis and site-directed mutagenesis of this promoter reveal two negative elements and one positive element. The positively-acting sequence includes one functional E-box. One of the repressor elements is located in the transcribed region and is the NRSE/RE1 sequence already described in promoters of neuronal genes. In this paper, we describe the neuron-specific promoter of the gene encoding the neuronal nicotinic acetylcholine receptor beta 2-subunit.
journal_name
Neurosciencejournal_title
Neuroscienceauthors
Bessis A,Salmon AM,Zoli M,Le Novère N,Picciotto M,Changeux JPdoi
10.1016/0306-4522(95)00303-zsubject
Has Abstractpub_date
1995-12-01 00:00:00pages
807-19issue
3eissn
0306-4522issn
1873-7544pii
0306-4522(95)00303-Zjournal_volume
69pub_type
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