Effects of cyclopiazonic acid on membrane currents, contraction and intracellular calcium transients in frog heart.


:The effects of cyclopiazonic acid, a specific inhibitor of the sarcoplasmic reticulum Ca(2+)-ATPase, on membrane currents and contraction were investigated under voltage clamped conditions on frog atrial trabeculae using the double mannitol-gap technique. In ringer solution, cyclopiazonic acid (10 microM) decreased the phasic contraction at all levels of depolarization without significant change in the time to peak of the contraction and time constant of relaxation. In a low-sodium the amplitude and the current/voltage relationship of L-type and T-type Ca2+ currents were not modified while their inactivation phase was markedly slowed by cyclopiazonic acid. At all levels of depolarization the tonic contraction was increased by cyclopiazonic acid. In the presence of cyclopiazonic acid, no significant variation in diastolic level of intracellular calcium ([Ca2+]i) was found while the maximum amplitude of the Ca2+ transient associated with an action potential was reduced and its time course slowed. All the reversible changes observed could be interpreted by assuming that, in frog atria, sarcoplasmic reticulum was present and cyclopiazonic acid inhibits the sarcoplasmic reticulum Ca(2+)-ATPase. It is proposed that under normal conditions relaxation is due mainly to Na+/Ca2+ exchange. The increase in [Ca2+]i during the contraction phase is due to ICa that provokes a fast release of Ca2+ from the sarcoplasmic reticulum and to an influx of Ca2+ provided by reverse Na+/Ca2+ exchange.


J Mol Cell Cardiol


Badaoui A,Huchet-Cadiou C,Léoty C




Has Abstract


1995-11-01 00:00:00














  • The effect of vitamin D3 deficiency on the isolated chick heart: hemodynamic, P-31 NMR and membrane studies.

    abstract::The purpose of this study was to investigate whether vitamin D3 deficiency affects the cardiac function of chick hearts directly or whether the influence is secondary through the hormone's effect on serum calcium levels. To this end, three experimental groups were studied: (a) the control group of vitamin D3 supplemen...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章


    authors: Hochhauser E,Kushnir T,Navon G,Rehavi M,Barak J,Edelstein S,Vidne B

    更新日期:1993-01-01 00:00:00

  • L-type calcium channel blockers and EGTA enhance superoxide production in cardiac fibroblasts.

    abstract::Since the recognition of the importance of calcium ions to cardiac contractility, the effects of alterations in calcium homeostasis on cardiac myocyte function have attracted immense attention. However, the possibility that changes in extracellular calcium concentration or the administration of calcium channel blocker...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章


    authors: Shivakumar K,Kumaran C

    更新日期:2001-02-01 00:00:00

  • Adenosine and dipyridamole mimic the effects of ischemic preconditioning.

    abstract::The effects of preconditioning, adenosine and dipyridamole in protecting the systolic and diastolic alterations of myocardial stunning in rabbit hearts were studied. Isovolumic left ventricular developed pressure (LVDP), and end diastolic pressure (LVEDP) were measured. The time constant of relaxation (T) was calculat...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章


    authors: Mosca SM,Gelpi RJ,Cingolani HE

    更新日期:1994-10-01 00:00:00

  • Type 2 diabetes, mitochondrial biology and the heart.

    abstract::Diabetes is recognized as an independent risk factor for cardiovascular morbidity and mortality. This is due, in large part, to premature atherosclerosis, enhanced thrombogenicity and activation of systemic inflammatory programs with resultant vascular dysfunction. More enigmatic mechanisms underpinning diabetes-assoc...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章,评审


    authors: Sack MN

    更新日期:2009-06-01 00:00:00

  • Some biochemical aspects of the protective effect of trimetazidine on rat cardiomyocytes during hypoxia and reoxygenation.

    abstract::This study was undertaken to evaluate the direct cardioprotective effect of trimetazidine (TMZ), an anti-anginal drug devoid of haemodynamic action, on isolated myocytes. Cultured rat ventricular myocytes were treated with the drug 16 h and 1 h before the experiments. The drug-treated cells and control cells were plac...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章


    authors: Fantini E,Demaison L,Sentex E,Grynberg A,Athias P

    更新日期:1994-08-01 00:00:00

  • Regional biochemical remodeling in non-infarcted tissue of rat heart post-myocardial infarction.

    abstract::Changes in the capacities of ATP-synthesizing reactions were analysed in residual non-infarcted myocardium following myocardial infarction. Rats were subjected to left coronary artery ligation (MI; n = 11) or to sham operation (sham; n = 18). Two months later, hearts were excised, rinsed and buffer-perfused isovolumic...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章


    authors: Laser A,Ingwall JS,Tian R,Reis I,Hu K,Gaudron P,Ertl G,Neubauer S

    更新日期:1996-07-01 00:00:00

  • Hic-5 deficiency enhances mechanosensitive apoptosis and modulates vascular remodeling.

    abstract::Forces associated with blood flow are crucial not only for blood vessel development but also for regulation of vascular pathology. Although there have been many studies characterizing the responses to mechanical stimuli, molecular mechanisms linking biological responses to mechanical forces remain unclear. Hic-5 (hydr...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章


    authors: Kim-Kaneyama JR,Takeda N,Sasai A,Miyazaki A,Sata M,Hirabayashi T,Shibanuma M,Yamada G,Nose K

    更新日期:2011-01-01 00:00:00

  • Magnesium-calcium exchange in cardiac troponin C bound to cardiac troponin I.

    abstract::Understanding the process of Ca(2+)/Mg(2+)exchange during muscle excitation and relaxation is fundamental to elucidating the mechanism of Ca(2+)-regulated muscle contraction. During the resting phase, the C-domain of cardiac troponin C may be occupied by either Ca(2+)or Mg(2+). Here, complexes of recombinant cardiac t...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章


    authors: Finley N,Dvoretsky A,Rosevear PR

    更新日期:2000-08-01 00:00:00

  • Increased constitutive nitric oxide production by whole body periodic acceleration ameliorates alterations in cardiomyocytes associated with utrophin/dystrophin deficiency.

    abstract::Duchenne Muscular Dystrophy (DMD) cardiomyopathy is a progressive lethal disease caused by the lack of the dystrophin protein in the heart. The most widely used animal model of DMD is the dystrophin-deficient mdx mouse; however, these mice exhibit a mild dystrophic phenotype with heart failure only late in life. In co...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章


    authors: Lopez JR,Kolster J,Zhang R,Adams J

    更新日期:2017-07-01 00:00:00

  • Altered cardiac mechanism and sarcoplasmic reticulum function in pressure overload-induced cardiac hypertrophy in rats.

    abstract::Cardiac sarcoplasmic reticulum (SR) sequesters Ca2+ and plays a crucial role in the regulation of intracellular Ca2+. Its functional properties are central to the excitation-contraction (E-C) cycle of cardiac muscle. In this study, we examined the hypothesis that alterations in SR function occur during the development...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章


    authors: Ohkusa T,Hisamatsu Y,Yano M,Kobayashi S,Tatsuno H,Saiki Y,Kohno M,Matsuzaki M

    更新日期:1997-01-01 00:00:00

  • Thymosin beta 4 treatment after myocardial infarction does not reprogram epicardial cells into cardiomyocytes.

    abstract::Myocardial infarction (MI) is one of the leading causes of morbidity and mortality world-wide. Whether endogenous repair and regenerative ability could be augmented by drug administration is an important issue for generation of novel therapeutic approach. Recently it was reported that in mice pretreated with thymosin ...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章


    authors: Zhou B,Honor LB,Ma Q,Oh JH,Lin RZ,Melero-Martin JM,von Gise A,Zhou P,Hu T,He L,Wu KH,Zhang H,Zhang Y,Pu WT

    更新日期:2012-01-01 00:00:00

  • Effects on recovery during acidosis in cardiac myocytes overexpressing CaMKII.

    abstract::Recovery of intracellular Ca transients and fractional shortening during late phase acidosis are suggested to be associated with CaMKII-dependent processes of which phospholamban (PLB) phosphorylation may play an important role. To test whether increased expression levels of CaMKII may further enhance recovery, we inv...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章


    authors: Sag CM,Dybkova N,Neef S,Maier LS

    更新日期:2007-12-01 00:00:00

  • Impaired Ca2+ cycling of nonischemic myocytes contributes to sarcomere dysfunction early after myocardial infarction.

    abstract::Changes in the nonischemic remote myocardium of the heart contribute to left ventricular dysfunction after ischemia and reperfusion (I/R). Understanding the underlying mechanisms early after I/R is crucial to improve the adaptation of the viable myocardium to increased mechanical demands. Here, we investigated the rol...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章


    authors: Kronenbitter A,Funk F,Hackert K,Gorreßen S,Glaser D,Boknik P,Poschmann G,Stühler K,Isić M,Krüger M,Schmitt JP

    更新日期:2018-06-01 00:00:00

  • Human cardiac phospholipase D activity is tightly controlled by phosphatidylinositol 4,5-bisphosphate.

    abstract::Phospholipase D (PLD) plays a central role in receptor-mediated breakdown of choline phospholipids and formation of phosphatidic acid (PA), an important regulator of cardiac function. However, specific mechanisms that regulate myocardial PLD activity remain largely unknown, particularly in the human heart. We hypothes...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章


    authors: Kurz T,Kemken D,Mier K,Weber I,Richardt G

    更新日期:2004-02-01 00:00:00

  • The feedback loop of "EMMPRIN/NF-κB" worsens atherosclerotic plaque via suppressing autophagy in macrophage.

    abstract::This study examined the significance of macrophage autophagy in extracellular matrix metalloproteinase inducer (EMMPRIN)-mediated atherosclerosis (AS). Apolipoprotein E-deficient (ApoE-/-) mice were fed a western diet to establish an AS model. EMMPRIN and p62/Sequestosome-1(SQSTM1) expression were evaluated in plaque ...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章


    authors: Liang X,Hou X,Yang Y,Liu H,Guo R,Yang Z,Yang L

    更新日期:2018-01-01 00:00:00

  • Enrichment of G protein alpha-subunit mRNAS in the AV-conducting system of the mammalian heart.

    abstract::We investigated the expression pattern of the heterotrimeric G proteins Gs alpha, Gi alpha-2 and Go alpha in rat and guinea-pig heart by in situ hybridization. Cryosections were hybridized with single-stranded 35S-cRNA probes complementary to subtype-specific sequences of the respective mRNAs. Hybridization signals we...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章


    authors: Eschenhagen T,Laufs U,Schmitz W,Scholz H,Warnholtz A,Weil J,Schäfer HJ

    更新日期:1995-10-01 00:00:00

  • Dual action of prajmalium on the Ca currents in frog isolated cardiomyocytes.

    abstract::The effects of N-n-propylajmaline (prajmalium) on the Na and Ca currents of single frog atrial and ventricular cells were studied by means of the whole-cell patch-clamp technique. Prajmalium (10(-9) to 10(-6) M) depressed the Na current (INa) in a dose- and use-dependent manner. In the same range of concentrations, pr...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章


    authors: Alvarez JL,Vassort G

    更新日期:1991-05-01 00:00:00

  • CaMKII effects on inotropic but not lusitropic force frequency responses require phospholamban.

    abstract::Increasing heart rate enhances cardiac contractility (force frequency relationship, FFR) and accelerates cardiac relaxation (frequency-dependent acceleration of relaxation, FDAR). The positive FFR together with FDAR promotes rapid filling and ejection of blood from the left ventricle (LV) at higher heart rates. Recent...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章


    authors: Wu Y,Luczak ED,Lee EJ,Hidalgo C,Yang J,Gao Z,Li J,Wehrens XH,Granzier H,Anderson ME

    更新日期:2012-09-01 00:00:00

  • Programmed death-ligand 1 triggers PASMCs pyroptosis and pulmonary vascular fibrosis in pulmonary hypertension.

    abstract::Pyroptosis is a pro-inflammatory form of programmed cell death, whose genesis directly depended on caspase-1 activation. Pulmonary hypertension (PH) is a disease characterized, in part, by vascular fibrosis. Up to now, there is no report on the relationship between pyroptosis and vascular fibrosis in PH. Here, we conf...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章


    authors: Zhang M,Xin W,Yu Y,Yang X,Ma C,Zhang H,Liu Y,Zhao X,Guan X,Wang X,Zhu D

    更新日期:2020-01-01 00:00:00

  • Effects of endurance training on cholinergic and adrenergic receptors of rat heart.

    abstract::To test the hypothesis that alterations in adrenergic or cholinergic receptors occur in response to physical training, and that changes in receptor properties could be mechanistically important in producting the altered cardiovascular physiology of the trained state, we studied the effects of endurance training by swi...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章


    authors: Williams RS,Schaible TF,Bishop T,Morey M

    更新日期:1984-05-01 00:00:00

  • Peptidyl-prolyl isomerase Pin1 deficiency attenuates angiotensin II-induced abdominal aortic aneurysm formation in ApoE-/- mice.

    abstract::Peptidyl-prolyl isomerase Pin1 has been reported to be associated with endothelial dysfunction. However, the role of smooth muscle Pin1 in the vascular system remains unclear. Here, we examined the potential function of Pin1 in smooth muscle cells (SMCs) and its contribution to abdominal aortic aneurysm (AAA) pathogen...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章


    authors: Liang ES,Cheng W,Yang RX,Bai WW,Liu X,Zhao YX

    更新日期:2018-01-01 00:00:00

  • NOR-1 modulates the inflammatory response of vascular smooth muscle cells by preventing NFκB activation.

    abstract::Recent work has highlighted the role of NR4A receptors in atherosclerosis and inflammation. In vascular smooth muscle cell (VSMC) proliferation, however, NOR-1 (neuron-derived orphan receptor-1) exerts antagonistic effects to Nur77 and Nurr1. The aim of this study was to analyse the effect of NOR-1 in VSMC inflammator...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章


    authors: Calvayrac O,Rodríguez-Calvo R,Martí-Pamies I,Alonso J,Ferrán B,Aguiló S,Crespo J,Rodríguez-Sinovas A,Rodríguez C,Martínez-González J

    更新日期:2015-03-01 00:00:00

  • Endothelium-targeted overexpression of constitutively active FGF receptor induces cardioprotection in mice myocardial infarction.

    abstract::Fibroblast growth factor receptor (FGFR) is expressed in a variety of cells and is involved in their proliferation/migration/survival. To elucidate FGFR-mediated specific action of vascular endothelial cells (ECs) on myocardial ischemia, we generated endothelium-targeted transgenic mice overexpressing constitutively a...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章


    authors: Matsunaga S,Okigaki M,Takeda M,Matsui A,Honsho S,Katsume A,Kishita E,Che J,Kurihara T,Adachi Y,Mansukhani A,Kobara M,Matoba S,Tatsumi T,Matsubara H

    更新日期:2009-05-01 00:00:00

  • Fatty acid regulation of glucose metabolism in the intact beating rat heart assessed by carbon-13 NMR spectroscopy: the critical role of pyruvate dehydrogenase.

    abstract::Although the myocardium is capable of utilizing both glucose and fatty acid substrates, glucose metabolism is inhibited in the presence of fatty acid during normal perfusion conditions. Fatty acid regulation of glucose utilization in intact beating rat hearts was studied with 13C-enriched substrates and 13C and 31P NM...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章


    authors: Weiss RG,Chacko VP,Gerstenblith G

    更新日期:1989-05-01 00:00:00

  • Mechanisms of calcium channel modulation by beta-adrenergic agents and dihydropyridine calcium agonists.

    abstract::Cardiac calcium channel activity is markedly increased by beta-adrenergic agents or calcium agonists such as Bay K 8644. The molecular mechanisms underlying these important modulatory effects have been studied with patch clamp techniques by several groups. This paper presents new experiments and reviews published evid...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章,评审


    authors: Tsien RW,Bean BP,Hess P,Lansman JB,Nilius B,Nowycky MC

    更新日期:1986-07-01 00:00:00

  • Aspirin and clopidogrel treatment impair nitric oxide biosynthesis by platelets.

    abstract::Aspirin and clopidogrel are used therapeutically for their anti-platelet effects. We examined the effects of aspirin and clopidogrel on basal and beta-adrenoceptor (beta-AR)-mediated platelet nitric oxide (NO) synthesis in healthy subjects and patients with coronary heart disease (CHD). Healthy subjects (n=19) were ra...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章,随机对照试验


    authors: O'Kane PD,Reebye V,Ji Y,Stratton P,Jackson G,Ferro A

    更新日期:2008-08-01 00:00:00

  • The effect of alterations to action potential duration on beta-adrenoceptor-mediated aftercontractions in human and guinea-pig ventricular myocytes.

    abstract::Aftercontractions induced by beta-adrenoceptor stimulation in human and guinea-pig cardiomyocytes may be related to changes in action potential duration (APD). We investigated the effects of altering APD during the occurrence of isoproterenol-induced aftercontractions, using the KATP channel openers cromakalim and lem...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章


    authors: Tweedie D,O'Gara P,Harding SE,MacLeod KT

    更新日期:1997-05-01 00:00:00

  • Myocardial adenosine formation during hypoxia: effects of ecto-5'-nucleotidase inhibition.

    abstract::Release of adenosine and AMP into epicardial fluid and coronary venous effluent of isovolumic guinea-pig hearts was examined during normoxic (95% O2) and hypoxic (30% O2) perfusion with and without the ecto-5'-nucleotidase inhibitor alpha,beta-methylene adenosine diphosphate (AOPCP)*. Normoxic epicardial and venous ad...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章


    authors: Headrick JP,Matherne GP,Berne RM

    更新日期:1992-03-01 00:00:00

  • Contribution of KChIP2 to the developmental increase in transient outward current of rat cardiomyocytes.

    abstract::The Ca(2+)-independent, voltage-gated transient outward current (I(to)) displays a marked increase during development of cardiomyocytes. However, the molecular mechanism remained unclear. In rat adult ventricular myocytes, I(to) can be divided into a fast (I(to,f)) and a slow (I(to,s)) component by recovery process fr...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章


    authors: Kobayashi T,Yamada Y,Nagashima M,Seki S,Tsutsuura M,Ito Y,Sakuma I,Hamada H,Abe T,Tohse N

    更新日期:2003-09-01 00:00:00

  • Generation of survival signal by differential interaction of p38MAPKalpha and p38MAPKbeta with caveolin-1 and caveolin-3 in the adapted heart.

    abstract::Sphingomyelin breakdown product ceramide has recently been found to induce an adaptive response and reduce myocardial ischemia/reperfusion injury. Since activation of MAP kinases plays an essential role in myocardial adaptation to ischemic stress and since ceramide is involved in lipid raft formation where MAP kinases...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章


    authors: Das M,Cui J,Das DK

    更新日期:2007-01-01 00:00:00