Circularly permuted interleukin 4 retains proliferative and binding activity.


:In human interleukin 4(IL-4), the carboxyl and amino termini of the 129 amino acid hormone are close to each other and this region is believed to be important for binding to the IL-4 receptor (IL-4r). We constructed plasmids encoding circularly permuted IL-4 mutants with the peptide Gly-Gly-Asn-Gly-Gly (GGNGG) joining the carboxyl to the amino terminus and with new amino and carboxyl termini elsewhere. Mutant IL-4(38-37) is composed of IL-4 residues 38-129 GGNGG and 1-37. Mutant IL-4(105-104) is composed of IL-4 residues 105-129, GGNGG and 1-104. IL-4(38-37) and IL-4(105-104) were purified from E. coli to near homogeneity and retained 50-100% of the binding and proliferative activity of IL-4, and in addition retained the ability to upregulate CD23 on Burkitt's lymphoma cells. Circular dichroism studies indicated that the tertiary structures of both IL-4(38-37) and IL-4(105-104) were retained, with the former molecule most similar to native IL-4. We conclude that while both native termini of IL-4 may be near its binding site, neither is required to be free for optimum activity.






Kreitman RJ,Puri RK,McPhie P,Pastan I




Has Abstract


1995-05-01 00:00:00
















  • Association between periodontitis and common variants in the promoter of the interleukin-6 gene.

    abstract::We recently reported an association between interleukin-6 (IL6) polymorphisms (SNPs) and haplotypes and aggressive periodontitis (AgP). The aim of this study was to investigate this association in a larger cohort of subjects, affected by either aggressive or chronic periodontitis. Five IL6 SNPs were analyzed in 765 su...


    pub_type: 杂志文章


    authors: Nibali L,D'Aiuto F,Donos N,Griffiths GS,Parkar M,Tonetti MS,Humphries SE,Brett PM

    更新日期:2009-01-01 00:00:00

  • Ccl22/MDC, is a prostaglandin dependent pyrogen, acting in the anterior hypothalamus to induce hyperthermia via activation of brown adipose tissue.

    abstract::CC Chemokine ligand 22 (Ccl22) is a selective, high affinity ligand at the CC chemokine receptor 4 (Ccr4). We have identified cDNAs encoding both ligand and receptor of the Ccl22-Ccr4 pair in cDNA libraries of the anterior hypothalamus/pre-optic area (AH/POA) by PCR. The AH/POA is the key brain region where endogenous...


    pub_type: 杂志文章


    authors: Osborn O,Sanchez-Alavez M,Dubins JS,Gonzalez AS,Morrison B,Hadcock JR,Bartfai T

    更新日期:2011-03-01 00:00:00

  • Chemotaxis and chemotactic selection induced with cytokines (IL-8, RANTES and TNF-alpha) in the unicellular Tetrahymena pyriformis.

    abstract::Three representative cytokines interleukin (IL-8), RANTES and tumour necrosis factor alpha (TNF-alpha) have a concentration-dependent chemotactic effect on the unicellular Tetrahymena. Maximal effective concentrations of IL-8 (1 ng/ml) and RANTES (75 ng/ml) are in the same range as in mammals, which indicates an evolu...


    pub_type: 杂志文章


    authors: Köhidai L,Csaba G

    更新日期:1998-07-01 00:00:00

  • Co-localization of Interleukin-1α and Annexin A2 at the plasma membrane in response to oxidative stress.

    abstract::Interleukin-1α (IL-1α) and Annexin A2 (AnxA2) are pleiotropic molecules with both intracellular and extracellular roles. They share several characteristics including unconventional secretion aided by S100 proteins, anchoring of the externalized proteins at the outer surface of the plasma membrane and response to oxida...


    pub_type: 杂志文章


    authors: Novák J,Vopálenský V,Pospíšek M,Vedeler A

    更新日期:2020-09-01 00:00:00

  • Interleukin-6 in neuro-Behçet's disease: association with disease subsets and long-term outcome.

    abstract::Increased cerebrospinal fluid (CSF) IL-6 has been reported in patients with Behçet's disease (BD) and neurological involvement. To elucidate the value of IL-6 as a marker of disease activity, serum and CSF IL-6 levels of 68 BD patients with acute (26) or chronic progressive (14) parenchymal involvement (pNB), dural si...


    pub_type: 杂志文章


    authors: Akman-Demir G,Tüzün E,Içöz S,Yeşilot N,Yentür SP,Kürtüncü M,Mutlu M,Saruhan-Direskeneli G

    更新日期:2008-12-01 00:00:00

  • Expression of interferon-γ, interferon-α and related genes in individuals with Down syndrome and periodontitis.

    abstract:BACKGROUND:Recently, attenuation of anti-inflammatory and increase of pro-inflammatory mediators was demonstrated in individuals with Down syndrome (DS) in comparison with euploid patients during periodontal disease (PD), suggesting a shift to a more aggressive inflammation in DS. AIM:To determine the influence of DS ...


    pub_type: 杂志文章


    authors: Tanaka MH,Giro EM,Cavalcante LB,Pires JR,Apponi LH,Valentini SR,Spolidório DM,Capela MV,Rossa C Jr,Scarel-Caminaga RM

    更新日期:2012-12-01 00:00:00

  • T-bet-/- RAG2-/- ulcerative colitis: the role of T-bet as a peacekeeper of host-commensal relationships.

    abstract::Inflammatory bowel disease is a disease that reflects a disequilibrium in host-commensal homeostasis. T-bet-/-xRAG2-/- deficient mice develop a spontaneous juvenile ulcerative colitis resulting from a pro-inflammatory response to the commensal microbiota that is dendritic cell and TNF-alpha driven [schematized in Fig....


    pub_type: 杂志文章,评审


    authors: Garrett WS,Glimcher LH

    更新日期:2009-10-01 00:00:00

  • Expression of several cytokines in prostate cancer: Correlation with clinical variables of patients. Relationship with biochemical progression of the malignance.

    abstract:BACKGROUND:This work is focused on finding new markers that complement or diagnoses currently used towards improving knowledge histological and statistical aspects that allow us to predict the local stage carcinomas and to identify and understand all the factors related to the progression of this disease. MATERIALS AN...


    pub_type: 临床试验,杂志文章


    authors: Torrealba N,Rodríguez-Berriguete G,Fraile B,Olmedilla G,Martínez-Onsurbe P,Guil-Cid M,Paniagua R,Royuela M

    更新日期:2017-01-01 00:00:00

  • Vascular endothelial growth factor, basic fibroblast growth factor, insulin-like growth factor-I and platelet-derived growth factor levels in human milk of mothers with term and preterm neonates.

    abstract::Human milk is a complex biological fluid. It contains many nutrients, anti-infectious and biologically active substance. Human milk also contains many angiogenic polypeptides. We have determined four of these: Vascular endothelial growth factor (VEGF), basic fibroblast growth factor (b-FGF), insulin-like growth factor...


    pub_type: 杂志文章


    authors: Ozgurtas T,Aydin I,Turan O,Koc E,Hirfanoglu IM,Acikel CH,Akyol M,Erbil MK

    更新日期:2010-05-01 00:00:00

  • Differential alterations in plasma colony-stimulating factor concentrations after coronary artery bypass graft surgery with extracorporeal circulation.

    abstract::To determine whether colony-stimulating factor (CSF) might participate to the inflammatory response after cardiac surgery, plasma concentrations of granulocyte-CSF (G-CSF), macrophage-CSF (M-CSF) and GM-CSF were measured in 31 patients undergoing coronary artery bypass graft (CABG) surgery with extracorporeal circulat...


    pub_type: 杂志文章


    authors: Denizot Y,Karoutsos S,Nathan N

    更新日期:2001-03-07 00:00:00

  • Intracellular localization of the p35 subunit of murine IL-12.

    abstract::Production of interleukin-12 (IL-12), a heterodimer of p35 and p40 subunits, is limited by p35 expression. A long and a short murine p35 mRNA potentially encoding proteins differing in pre-sequence size are produced. Increased pre-sequence size could convert a cleaved signal peptide to an uncleaved signal peptide, rai...


    pub_type: 杂志文章


    authors: Vaidyanathan H,Zhou Y,Petro TM,Schwartzbach SD

    更新日期:2003-02-07 00:00:00

  • Biological activity of ovine IL-23 expressed using a foot-and-mouth disease virus 2A self-cleaving peptide.

    abstract::Hetero-dimeric cytokines often require equi-molar expression of both subunits to achieve biological activity. Previously, we expressed ovine IL-12 p40 and p35 linked using a self-cleaving 2A peptide from foot-and-mouth disease virus (FMDV). We now generated a new improved vector for the expression of hetero-dimeric cy...


    pub_type: 杂志文章


    authors: Yen HH,Scheerlinck JP

    更新日期:2013-03-01 00:00:00

  • Candida albicans SC5314 inhibits NLRP3/NLRP6 inflammasome expression and dampens human intestinal barrier activity in Caco-2 cell monolayer model.

    abstract::Candida albicans is an opportunistic fungal pathogen that colonizes human gastro-intestinal mucosal tissues. Its effect on the immune response in intestinal epithelial cells and on the intestinal mucosal barrier are not yet fully understood. In this study, we investigated Caco-2 cells, a monolayer model of intestinal ...


    pub_type: 杂志文章


    authors: Mao X,Qiu X,Jiao C,Lu M,Zhao X,Li X,Li J,Ma J,Zhang H

    更新日期:2020-02-01 00:00:00

  • VEGF gene therapy cooperatively recruits molecules from the immune system and stimulates cell homing and angiogenesis in refractory angina.

    abstract:BACKGROUND:New vessels are formed in response to stimuli from angiogenic factors, a process in which paracrine signaling is fundamental. OBJECTIVE:To investigate the cooperative paracrine signaling profile in response to Vascular Endothelial Growth Factor (VEGF) gene therapy in patients with coronary artery disease (C...


    pub_type: 临床试验,杂志文章


    authors: Eibel B,Markoski MM,Rodrigues CG,Dipp T,de Salles FB,Giusti II,Nardi NB,Plentz RD,Kalil RA

    更新日期:2017-03-01 00:00:00

  • IL-33 stimulates expression of the GPR84 (EX33) fatty acid receptor gene and of cytokine and chemokine genes in human adipocytes.

    abstract::Expression of GPCR fatty acid sensor/receptor genes in adipocytes is modulated by inflammatory mediators, particularly IL-1β. In this study we examined whether the IL-1 gene superfamily member, IL-33, also regulates expression of the fatty acid receptor genes in adipocytes. Human fat cells, differentiated from preadip...


    pub_type: 杂志文章


    authors: Zaibi MS,Kępczyńska MA,Harikumar P,Alomar SY,Trayhurn P

    更新日期:2018-10-01 00:00:00

  • Insulin-like growth factor-1 binding protein 3 (IGFBP-3) promotes recovery from trauma-induced expression of inflammatory and apoptotic factors in retina.

    abstract::Ocular trauma affects 20% of Americans in their lifetime and can cause permanent visual system damage. We have used a mouse model of ocular trauma (exposure to an air blast from a paintball gun) to examine pathways that trigger the resulting retinal damage and to develop treatment strategies that might ameliorate the ...


    pub_type: 杂志文章


    authors: Jiang Y,Pagadala J,Miller DD,Steinle JJ

    更新日期:2014-12-01 00:00:00

  • Serum VEGF levels and tissue activation of VEGFR2/KDR receptors in patients with breast and gynecologic cancer.

    abstract:OBJECTIVES:Vascular endothelial cell growth factor (VEGF) plays an important role in the biology of gynecological cancer, usually linked with aggressive tumour behaviour and a poor postoperative outcome. Yet, its role in benign breast/gynecological conditions is less clear. METHODS:Serum VEGF was analysed in a series ...


    pub_type: 杂志文章


    authors: Koukourakis MI,Limberis V,Tentes I,Kontomanolis E,Kortsaris A,Sivridis E,Giatromanolaki A

    更新日期:2011-03-01 00:00:00

  • Macrophage activation for the production of immunostimulatory cytokines by delivering interleukin 1 via biodegradable microspheres.

    abstract::Interleukin 1alpha (IL-1alpha), a pleiotropic cytokine with multiple anti-tumour activities, has been investigated in our laboratory for its potential to serve as an immunotherapeutic agent. In the present study, an attempt was made to direct IL-1alpha to macrophages, in order to induce their immunoregulatory activiti...


    pub_type: 杂志文章


    authors: Mullerad J,Cohen S,Voronov E,Apte RN

    更新日期:2000-11-01 00:00:00

  • A human cell line isolated from fetal kidney produces an apparently erythroid-specific stimulating activity.

    abstract::The burst formation from human and murine burst forming unit-erythroid (BFU-E) requires the presence of erythropoietin (Epo) in semi-solid cultures of bone marrow cells. A number of haematopoietic factors are described that increase the burst number: interleukin 3 (IL-3), stem cell factor (SCF), granulocyte-macrophage...


    pub_type: 杂志文章


    authors: Rodriguez MH,Arnaud S,Geay T,Mouchiroud G,Grasset MF,Blanchet JP

    更新日期:1998-08-01 00:00:00

  • The role of complement activation in tumour necrosis factor-induced lethal hepatitis.

    abstract::Injection of tumour necrosis factor (TNF) in animals causes severe liver cell toxicity, especially when D-(+)-galactosamine (GalN) is co-administered. After challenge with TNF/GalN, serum complement activity (CH50 and APCH50) decreased dramatically, suggesting strong activation of both the classical and the alternativ...


    pub_type: 杂志文章


    authors: Libert C,Wielockx B,Grijalba B,Van Molle W,Kremmer E,Colten HR,Fiers W,Brouckaert P

    更新日期:1999-08-01 00:00:00

  • Differences in biomarkers of inflammation and immune responses in chronic smokers and moist snuff users.

    abstract:BACKGROUND:Cigarette smoking is a major risk factor for cancer and other diseases. While smoking induces chronic inflammation and aberrant immune responses, the effects of smokeless tobacco products (STPs) on immune responses is less clear. Here we evaluated markers related to immune regulation in smokers (SMK), moist ...


    pub_type: 杂志文章


    authors: Tran QT,Arimilli S,Scott E,Chen P,Prasad GL

    更新日期:2021-01-01 00:00:00

  • Characterization of a subclone (D10S) of the D10.G4.1 helper T-cell line which proliferates to attomolar concentrations of interleukin-1 in the absence of mitogens.

    abstract::Most studies have shown that interleukin-1 (IL-1) acts as a helper or co-stimulator in T-lymphocyte activation and proliferation by mitogens or antigens. We describe here a stable subclone (D10S) of the murine D10.G4.1 helper T-cell which proliferates to subfemtomolar (attomolar) concentrations of IL-1 beta or alpha i...


    pub_type: 杂志文章


    authors: Orencole SF,Dinarello CA

    更新日期:1989-11-01 00:00:00

  • The interplay between cytokines and the Kynurenine pathway in inflammation and atherosclerosis.

    abstract::The kynurenine pathway (KP) is the major metabolic route of tryptophan (Trp) metabolism. Indoleamine 2,3-dioxygenase (IDO1), the enzyme responsible for the first and rate-limiting step in the pathway, as well as other enzymes in the pathway, have been shown to be highly regulated by cytokines. Hence, the KP has been i...


    pub_type: 杂志文章,评审


    authors: Baumgartner R,Forteza MJ,Ketelhuth DFJ

    更新日期:2019-10-01 00:00:00

  • Interferon alpha induces generation of semi-mature dendritic cells with high pro-inflammatory and cytotoxic potential.

    abstract::Dendritic cell-based vaccines are considered as a new and promising immunotherapeutic approach for cancer treatment. However, the choice of optimal protocol of dendritic cell generation in vitro represents the major challenge. Here, we compared phenotype and functional characteristics of human monocyte-derived dendrit...


    pub_type: 杂志文章


    authors: Leplina OY,Tyrinova TV,Tikhonova MA,Ostanin AA,Chernykh ER

    更新日期:2015-01-01 00:00:00

  • TNF-alpha and IL-8: serum levels and gene polymorphisms (-308G>A and -251A>T) are associated with classical biomarkers and medical history in children with sickle cell anemia.

    abstract::Sickle cell anemia (SCA) is a disorder characterized by a heterogeneous clinical outcome. In the present study, we investigated the associations between Tumor Necrosis Factor-alpha (TNF-alpha) -308G>A and Interleukin 8 (IL-8) -251A>T gene polymorphisms, medical history and classical biomarkers in children with steady-...


    pub_type: 杂志文章


    authors: Cajado C,Cerqueira BA,Couto FD,Moura-Neto JP,Vilas-Boas W,Dorea MJ,Lyra IM,Barbosa CG,Reis MG,Goncalves MS

    更新日期:2011-11-01 00:00:00

  • Levels of pro- and anti-inflammatory cytokines in cystic fibrosis patients with or without gingivitis.

    abstract:BACKGROUND:Inflammatory periodontal diseases are caused by interaction between gram negative, anaerobic bacteria and host response. Persistent infection of Pseudomonas aeruginosa in cystic fibrosis (CF) patients also cause increased pro-inflammatory response and the imbalance of pro- and anti-inflammatory response in b...


    pub_type: 杂志文章


    authors: Duruel O,Berker E,Özşin-Özler C,Gharibzadeh-Hızal M,Gürpınar Ö,Eryılmaz-Polat S,Ataman-Duruel ET,Tan Ç,Karabulut E,Tekçiçek M,Eser ÖK,Kiper N,Tezcan İ

    更新日期:2020-03-01 00:00:00

  • IL-17/Th17 targeting: on the road to prevent chronic destructive arthritis?

    abstract::Interleukin-17A (IL-17A) contributes to the pathogenesis of arthritis. Data from experimental arthritis indicate IL-17 receptor signaling as a critical pathway in turning an acute synovitis into a chronic destructive arthritis. The identification of six IL-17 family members (IL-17A-F) may extend the role of this novel...


    pub_type: 杂志文章,评审


    authors: Lubberts E

    更新日期:2008-02-01 00:00:00

  • Association between the IFNG +874A/T gene polymorphism and leprosy resistance: a meta-analysis.

    abstract::Previous studies identified the variant IFNG +874A/T (rs2430561) in the first intron of the gene in association with mycobacterial infection, especially tuberculosis and leprosy. The aim of this investigation was to analyze the protective role of the T allele in relation to leprosy using a meta-analysis evaluation. Th...


    pub_type: 杂志文章,meta分析


    authors: Silva GA,Naveca FG,Ramasawmy R,Boechat AL

    更新日期:2014-02-01 00:00:00

  • Interleukin-1F7B (IL-1H4/IL-1F7) is processed by caspase-1 and mature IL-1F7B binds to the IL-18 receptor but does not induce IFN-gamma production.

    abstract::We have recently reported the identification of four novel members of the interleukin-1 (IL-1) family which we designated as IL-1 homologue 1-4 (IL-1H1-4). These proteins exhibit significant sequence homology to other members of the IL-1 family. Of these homologues, only IL-1H4 (renamed IL-1F7b) was predicted to conta...


    pub_type: 杂志文章


    authors: Kumar S,Hanning CR,Brigham-Burke MR,Rieman DJ,Lehr R,Khandekar S,Kirkpatrick RB,Scott GF,Lee JC,Lynch FJ,Gao W,Gambotto A,Lotze MT

    更新日期:2002-04-21 00:00:00

  • Transforming growth factor beta1 and soluble Fas serum levels in hepatocellular carcinoma.

    abstract::In this study we assessed the usefulness of serum Transforming Growth Factor-beta1 (TGF-beta1) and soluble Fas (sFas) in distinguishing liver cirrhosis (LC) with and without hepatocellular carcinoma (HCC) as compared with alpha-fetoprotein (AFP). Serum TGF-beta1 and sFas levels were measured by ELISA in 51 LC patients...


    pub_type: 杂志文章


    authors: Sacco R,Leuci D,Tortorella C,Fiore G,Marinosci F,Schiraldi O,Antonaci S

    更新日期:2000-06-01 00:00:00