Investigation of the stability of apple allergen extracts.


:To determine optimal conditions for allergen preservation, we investigated the influence of different stabilizing additives and of storage temperature on the allergen activity of apple protein preparations, obtained by extraction in phosphate buffer or by precipitation in diacetone alcohol and resolubilization in phosphate buffer in the presence or absence of enzyme inhibitors. For this purpose, the extracts were stored for 6 months either in frozen state at -20 degrees C or in lyophilized state at -20 degrees C, 4 degrees C, or room temperature and were characterized by SDS-PAGE, immunoblot, ELISA inhibition, and prick test. The highest stability revealed the extracts that were prepared by precipitation in the organic solvent in the presence of enzyme inhibitors, lyophilized, and stored at -20 degrees C. For storage of extract solutions at 4 degrees C, PBS/glycerol and cysteine/sodium citrate/glycerol were found to be the most effective stabilizing additives.






Rudeschko O,Fahlbusch B,Henzgen M,Schlenvoigt G,Herrmann D,Vieths S,Jäger L




Has Abstract


1995-07-01 00:00:00














  • Ultrastructural localization of histamine in human basophils and mast cells; changes associated with anaphylactic degranulation and recovery demonstrated with a diamine oxidase-gold probe.

    abstract::Subcellular sites of histamine in purified human lung mast cells and human peripheral blood basophils were determined using a newly developed enzyme-gold affinity method for ultrastructural analysis. The evolving morphologies of regulated secretion and recovery from regulated secretion in each cell showed that granule...


    pub_type: 杂志文章,评审


    authors: Dvorak AM

    更新日期:1997-01-01 00:00:00

  • Neonatal leukocyte cAMP-phosphodiesterase determination is not suitable for allergy prediction.

    abstract::The atopy-predictive value of cord blood (CB) cyclic adenosine monophosphate-phosphodiesterase (cAMP-PDE) in mononuclear leukocytes (MN) was studied prospectively in 57 neonates. Reliable determination of cAMP-PDE was achieved in only 20 CB samples. The levels tended to be higher in infants developing signs of atopy u...


    pub_type: 杂志文章


    authors: Odelram H,Björkstén B,Chan SC,Hanifin JM,Kjellman NI

    更新日期:1994-09-01 00:00:00

  • Long-term evaluation of usefulness of skin and incremental challenge tests in patients with history of adverse reaction to local anesthetics.

    abstract::A variety of adverse reactions to local anesthetics has been described, some of which are thought to be allergic. Different protocols of prick and intradermal skin tests as well as subcutaneous challenge tests are used to select a local anesthetic which can safely be used. Their long-term effectiveness has not yet bee...


    pub_type: 杂志文章


    authors: Wasserfallen JB,Frei PC

    更新日期:1995-02-01 00:00:00

  • Once daily fluticasone propionate aqueous nasal spray controls symptoms of most patients with seasonal allergic rhinitis.

    abstract::This multicentre, randomized, double-blind, parallel-group study was designed to compare the efficacy and tolerability of fluticasone propionate aqueous nasal spray 200 micrograms once daily (FPANS 200 micrograms od) with FPANS 200 micrograms twice daily (bd) in patients whose seasonal rhinitis symptoms were not compl...


    pub_type: 临床试验,杂志文章,多中心研究,随机对照试验


    authors: Pedersen B,Dahl R,Richards DH,Jacques LA,Larsen BB,Pichler W,Nykanen KN

    更新日期:1995-10-01 00:00:00

  • Current perspective on eicosanoids in asthma and allergic diseases: EAACI Task Force consensus report, part I.

    abstract::Eicosanoids are biologically active lipid mediators, comprising prostaglandins, leukotrienes, thromboxanes, and lipoxins, involved in several pathophysiological processes relevant to asthma, allergies, and allied diseases. Prostaglandins and leukotrienes are the most studied eicosanoids and established inducers of air...


    pub_type: 杂志文章


    authors: Sokolowska M,Rovati GE,Diamant Z,Untersmayr E,Schwarze J,Lukasik Z,Sava F,Angelina A,Palomares O,Akdis CA,O'Mahony L,Sanak M,Dahlen SE,Woszczek G

    更新日期:2021-01-01 00:00:00

  • Lipid and protein metabolism in asthma. Effects of diet and corticosteroid therapy.

    abstract:BACKGROUND:Because little is known about the effects of asthma and asthma therapy on lipid and protein metabolism, we have investigated the characteristics of diet and plasma/serum levels of fat and protein in a group of asthma patients with various degrees of severity. METHODS:A case-control study was carried out on ...


    pub_type: 杂志文章


    authors: Picado C,Deulofeu R,Lleonart R,Agustí M,Casals E,Quintó L,Mullol J

    更新日期:1999-06-01 00:00:00

  • Kiwifruit Act d 11 is the first member of the ripening-related protein family identified as an allergen.

    abstract:BACKGROUND:Kiwifruit is an important cause of food allergy. A high amount of a protein with a molecular mass compatible with that of Bet v 1 was observed in the kiwifruit extract. OBJECTIVE:To identify and characterize kirola, the 17-kDa protein of green kiwifruit (Act d 11). METHODS:Act d 11 was purified from green ...


    pub_type: 杂志文章


    authors: D'Avino R,Bernardi ML,Wallner M,Palazzo P,Camardella L,Tuppo L,Alessandri C,Breiteneder H,Ferreira F,Ciardiello MA,Mari A

    更新日期:2011-07-01 00:00:00

  • Genetic and environmental factors in the changing incidence of allergy.

    abstract::Several studies have indicated that the incidence and prevalence of allergic diseases are increasing. Such data should not be regarded as mere statistical curiosities, but should be analysed to provide information on the factors contributing to allergy and its changing epidemiology. Extensive evidence has been accumul...


    pub_type: 杂志文章


    authors: Bonini S,Magrini L,Rotiroti G,Ronchetti MP,Onorati P

    更新日期:1994-01-01 00:00:00

  • Suppression of adrenomedullin contributes to vascular leakage and altered epithelial repair during asthma.

    abstract:BACKGROUND:The anti-inflammatory peptide, adrenomedullin (AM), and its cognate receptor are expressed in lung tissue, but its pathophysiological significance in airway inflammation is unknown. OBJECTIVES:This study investigated whether allergen-induced airway inflammation involves an impaired local AM response. METHO...


    pub_type: 杂志文章


    authors: Hagner S,Welz H,Kicic A,Alrifai M,Marsh LM,Sutanto EN,Ling KM,Stick SM,Müller B,Weissmann N,Renz H

    更新日期:2012-08-01 00:00:00

  • Allergic rhinitis and inflammation: the effect of nasal corticosteroid therapy.

    abstract::Topical corticosteroids have proved to be effective in the treatment of allergic rhinitis. The symptomatology of allergic rhinitis is considered to be the result of the accumulation and activation of inflammatory cells and cytokine release and hence the efficacy of corticosteroids is associated with their anti-inflamm...


    pub_type: 杂志文章,评审


    authors: Fokkens WJ,Godthelp T,Holm AF,Blom H,Klein-Jan A

    更新日期:1997-01-01 00:00:00

  • Chaperone patterns in vernal keratoconjunctivitis are distinctive of cell and Hsp type and are modified by inflammatory stimuli.

    abstract:BACKGROUND:Vernal keratoconjunctivitis (VKC) is a severe ocular allergy with pathogenic mechanism poorly understood and no efficacious treatment. The aims of the study were to determine quantities and distribution of Hsp chaperones in the conjunctiva of VKC patients and assess their levels in conjunctival epithelial an...


    pub_type: 杂志文章


    authors: Leonardi A,Tarricone E,Corrao S,Alaibac M,Corso AJ,Zavan B,Venier P,Conway de Macario E,Macario AJ,Di Stefano A,Cappello F,Brun P

    更新日期:2016-03-01 00:00:00

  • Challenges in the implementation of EAACI guidelines on allergen immunotherapy: A global perspective on the regulation of allergen products.

    abstract::Regulatory approaches for allergen immunotherapy (AIT) products and the availability of high-quality AIT products are inherently linked to each other. While allergen products are available in many countries across the globe, their regulation is very heterogeneous. First, we describe the regulatory systems applicable f...


    pub_type: 杂志文章,评审


    authors: Bonertz A,Roberts GC,Hoefnagel M,Timon M,Slater JE,Rabin RL,Bridgewater J,Pini C,Pfaar O,Akdis C,Goldstein J,Poulsen LK,van Ree R,Rhyner C,Barber D,Palomares O,Sheikh A,Pawankar R,Hamerlijnk D,Klimek L,Agache I,

    更新日期:2018-01-01 00:00:00

  • The size of the disease relevant IgE antibody fraction in relation to 'total-IgE' predicts the efficacy of anti-IgE (Xolair) treatment.

    abstract:BACKGROUND:Some patients with allergic asthma treated with anti-IgE (Xolair) do not become symptom free. Better criteria for response assessment than allergy skin tests or IgE determination are needed. The impact of the size of the disease relevant allergen-specific IgE antibody fraction, i.e. the percentage of IgE ant...


    pub_type: 杂志文章,随机对照试验


    authors: Johansson SG,Nopp A,Oman H,Ankerst J,Cardell LO,Grönneberg R,Matsols H,Rudblad S,Strand V,Stålenheim G

    更新日期:2009-10-01 00:00:00

  • A high-positive patch test load correlates with further positive patch test reactions irrespective of their location.

    abstract:BACKGROUND:Patch testing is the standard clinical procedure to prove contact sensitization. It is a common practice to attach multiple patch tests at the same time. However, synchronous reactions to unrelated allergens may not be completely unassociated. If so, the reaction in a given test field might be influenced by ...


    pub_type: 杂志文章


    authors: Brasch J,Geier J,Schnuch A,Uter W,German Contact Dermatitis Research Group (DKG).,Information Network of Departments of Dermatology (IVDK).

    更新日期:2006-12-01 00:00:00

  • Prenatal allergen exposures prevent allergen-induced sensitization and airway inflammation in young mice.

    abstract:BACKGROUND:Immune-modulation such as tolerance induction appears to be an upcoming concept to prevent development of atopic diseases. Pregnancy might present a critical period for preventing allergic sensitization of the progeny. We investigated the effect of maternal allergen exposures during pregnancy on allergen-ind...


    pub_type: 杂志文章


    authors: Gerhold K,Avagyan A,Reichert E,Seib C,Van DV,Luger EO,Hutloff A,Hamelmann E

    更新日期:2012-03-01 00:00:00

  • Immunostimulatory DNA-based therapeutics for experimental and clinical allergy.

    abstract::Although allergen immunotherapy (IT) has been shown to modulate allergic hypersensitivities, its efficacy is limited. Recently, in various models of experimental allergy, a number of reagents which we have termed immunostimulatory DNA-based therapeutics have proven highly effective in both the prevention and reversal ...


    pub_type: 杂志文章,评审


    authors: Horner AA,Takabaysahi K,Zubeldia JM,Raz E

    更新日期:2002-01-01 00:00:00

  • Myeloid-derived suppressor cell-like fibrocytes are increased and associated with preserved lung function in chronic obstructive pulmonary disease.

    abstract:BACKGROUND:The role of fibrocytes in chronic obstructive pulmonary disease (COPD) is unknown. We sought to enumerate blood and tissue fibrocytes in COPD and determine the association of blood fibrocytes with clinical features of disease. METHODS:Utilizing flow cytometry to identify circulating, collagen type 1+ cells,...


    pub_type: 杂志文章


    authors: Wright AK,Newby C,Hartley RA,Mistry V,Gupta S,Berair R,Roach KM,Saunders R,Thornton T,Shelley M,Edwards K,Barker B,Brightling CE

    更新日期:2017-04-01 00:00:00

  • Alpine climate treatment of atopic dermatitis: a systematic review.

    abstract::Climate therapy has been used for decades in the treatment of atopic dermatitis (AD), but evidence of its effectiveness has not yet been assessed systematically. A systematic literature search in Medline, Embase, and the Cochrane library was performed to identify all original studies concerning alpine climate treatmen...


    pub_type: 杂志文章,meta分析,评审


    authors: Fieten KB,Weststrate AC,van Zuuren EJ,Bruijnzeel-Koomen CA,Pasmans SG

    更新日期:2015-01-01 00:00:00

  • Oral allergy syndrome (OAS) in pollinosis patients after eating pistachio nuts: two cases with two different patterns of onset.

    abstract::We describe two uncommon cases of oral allergy syndrome (OAS) after eating pistachio nuts (p.n.) in subjects (a 54-year-old man and a 3-year-old girl) with exclusive skin prick test (SPT) positivity to Parietaria (P.) and pistachio nut (p.n.) allergens. Serologic P.- and p.n.-specific IgE determinations were also carr...


    pub_type: 杂志文章


    authors: Liccardi G,Mistrello G,Noschese P,Falagiani P,D'Amato M,D'Amato G

    更新日期:1996-12-01 00:00:00

  • Repeatability of peak nasal inspiratory flow measurements and utility for assessing the severity of rhinitis.

    abstract:BACKGROUND:The measurement of peak nasal inspiratory flow (PNIF) provides a simple, cheap, fast and readily available tool for determining the extent of nasal airway patency. However, there are questions regarding its repeatability when used to assess the degree of nasal obstruction in large populations. Therefore, thi...


    pub_type: 杂志文章


    authors: Starling-Schwanz R,Peake HL,Salome CM,Toelle BG,Ng KW,Marks GB,Lean ML,Rimmer SJ

    更新日期:2005-06-01 00:00:00

  • n-Nonanoyl-CCL14 (NNY-CCL14), a novel inhibitor of allergic airway inflammation is a partial agonist of human CCR2.

    abstract:BACKGROUND:Modulation of leukocyte recruitment through blocking of chemokine receptors has been proposed as an attractive therapeutic strategy. We have previously demonstrated that n-Nonanoyl-CC chemokine ligand 14 (NNY-CCL14), a modified analog of the naturally occurring chemokine CCL14(9-74) internalizes and desensit...


    pub_type: 杂志文章


    authors: Gupta S,Erdmann G,Schulz-Maronde S,Escher SE,Richter R,Forssmann WG,Elsner J,Forssmann U

    更新日期:2008-10-01 00:00:00

  • Cell-mediated immunity of Yersinia enterocolitica serotype 3 in patients with thyroid diseases.

    abstract::The cellular immunity to Yersinia enterocolitica serotype 3 and crude human thyroid extract in 64 patients with thyroid diseases and 25 controls was studied by the leucocyte migration test. In the patient group as a whole and in patients with Graves' disease and nontoxic diffuse goitre a significantly reduced leucocyt...


    pub_type: 杂志文章


    authors: Bech K,Clemmensen O,Larsen JH,Thyme S,Bendixen G

    更新日期:1978-04-01 00:00:00

  • Impact of (bio)chemical and physical procedures on food allergen stability.

    abstract::A variety of methods have been used to attempt to decrease the allergenicity of food products, with highly variable success. Limited knowledge of allergen and epitope structures and the factors governing their stability may explain this, and the ensuing need for an empirical approach. A combined effort from a processi...


    pub_type: 杂志文章,评审


    authors: Soler-Rivas C,Wichers HJ

    更新日期:2001-01-01 00:00:00

  • Advances and novel developments in environmental influences on the development of atopic diseases.

    abstract::Although genetic factors play a role in the etiology of atopic disease, the rapid increases in the prevalence of these diseases over the last few decades suggest that environmental, rather than genetic factors are the driving force behind the increasing prevalence. In modern societies, there is increased time spent in...


    pub_type: 杂志文章,评审


    authors: Alkotob SS,Cannedy C,Harter K,Movassagh H,Paudel B,Prunicki M,Sampath V,Schikowski T,Smith E,Zhao Q,Traidl-Hoffmann C,Nadeau KC

    更新日期:2020-10-09 00:00:00

  • Effects of ketotifen on symptoms and on bronchial mucosa in patients with atopic asthma.

    abstract::Ketotifen is marketed throughout the world as an antiallergy drug, but whether it affects infiltration of inflammatory cells into airway mucosa is not known. We studied the effects of ketotifen on symptoms, pulmonary function, and airway inflammation in 25 patients with atopic asthma. Patients took ketotifen (1 mg twi...


    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Hoshino M,Nakamura Y,Shin Z,Fukushima Y

    更新日期:1997-08-01 00:00:00

  • Long-term reduction in local inflammation by a lipid raft molecule in atopic dermatitis.

    abstract:BACKGROUND:The complex pathogenesis of atopic dermatitis (AD) is guided by cell surface receptor-mediated signal transduction regulated in lipid rafts. Miltefosine is a raft-modulating molecule targeting cell membranes. With this controlled clinical study, the clinical and immunomodulatory efficacy of miltefosine was i...


    pub_type: 杂志文章,随机对照试验


    authors: Dölle S,Hoser D,Rasche C,Loddenkemper C,Maurer M,Zuberbier T,Worm M

    更新日期:2010-09-01 00:00:00

  • National clinical practice guidelines for allergen immunotherapy: An international assessment applying AGREE-II.

    abstract:BACKGROUND:Since 1988, numerous allergen immunotherapy guidelines (AIT-GLs) have been developed by national and international organizations to guide physicians in AIT. Even so, AIT is still severely underused. OBJECTIVE:To evaluate AIT-GLs with AGREE-II, developed in 2010 by McMaster University methodologists to compr...


    pub_type: 杂志文章


    authors: Larenas-Linnemann DES,Antolín-Amérigo D,Parisi C,Nakonechna A,Luna-Pech JA,Wedi B,Davila I,Gómez M,Levin M,Ortega Martell JA,Klimek L,Rosario N,Muraro AM,Agache I,Bousquet J,Sheikh A,EAACI International Societies Council.

    更新日期:2018-03-01 00:00:00

  • Staphylococcus aureus-derived extracellular vesicles induce neutrophilic pulmonary inflammation via both Th1 and Th17 cell responses.

    abstract:BACKGROUND:Recent evidence indicates that Staphylococcus aureus, one of the most important human pathogens, secretes vesicles into the extracellular milieu. OBJECTIVE:To evaluate whether inhalation of S. aureus-derived extracellular vesicles (EV) is causally related to the pathogenesis of inflammatory pulmonary diseas...


    pub_type: 杂志文章


    authors: Kim MR,Hong SW,Choi EB,Lee WH,Kim YS,Jeon SG,Jang MH,Gho YS,Kim YK

    更新日期:2012-10-01 00:00:00

  • Allergic disorders in African countries: linking immunology to accurate phenotype.

    abstract::Identification and characterization of risk and protective factors for allergy is important for developing strategies for prevention or treatment. The prevalence of allergy is clearly higher in affluent countries than in developing countries like, e.g. Africa. Especially in urban areas of developing countries, allergy...


    pub_type: 杂志文章,评审


    authors: van Ree R,Yazdanbakhsh M

    更新日期:2007-03-01 00:00:00

  • Epidemiology of asthma in western Europe.

    abstract::Asthma deaths are uncommon, but have recently increased in some countries due to problems in the management of the disease. Morbidity rates show large variations, which can be attributed to differences in defining the disease, but also to genuine variations, with a trend towards less asthma in northern Europe. It has ...


    pub_type: 杂志文章,评审


    authors: Charpin D,Vervloet D,Charpin J

    更新日期:1988-10-01 00:00:00