Investigation of the stability of apple allergen extracts.


:To determine optimal conditions for allergen preservation, we investigated the influence of different stabilizing additives and of storage temperature on the allergen activity of apple protein preparations, obtained by extraction in phosphate buffer or by precipitation in diacetone alcohol and resolubilization in phosphate buffer in the presence or absence of enzyme inhibitors. For this purpose, the extracts were stored for 6 months either in frozen state at -20 degrees C or in lyophilized state at -20 degrees C, 4 degrees C, or room temperature and were characterized by SDS-PAGE, immunoblot, ELISA inhibition, and prick test. The highest stability revealed the extracts that were prepared by precipitation in the organic solvent in the presence of enzyme inhibitors, lyophilized, and stored at -20 degrees C. For storage of extract solutions at 4 degrees C, PBS/glycerol and cysteine/sodium citrate/glycerol were found to be the most effective stabilizing additives.






Rudeschko O,Fahlbusch B,Henzgen M,Schlenvoigt G,Herrmann D,Vieths S,Jäger L




Has Abstract


1995-07-01 00:00:00














  • Endocrine disrupters--potential modulators of the immune system and allergic response.

    abstract::Endocrine disrupters (EDs) are environmental pollutants of industrial or agricultural origin (e.g. herbicides, fungicides, insecticides, industrial chemicals) that may influence health of wildlife and human. Endocrine-disrupting effect is obtained by mimicking the action of the steroid hormones and has been associated...


    pub_type: 杂志文章,评审


    authors: Chalubinski M,Kowalski ML

    更新日期:2006-11-01 00:00:00

  • Nonatopic wheezy children have reduced interferon-gamma.

    abstract::Viruses cause asthmatic exacerbations in schoolchildren. We tested the hypothesis that children who wheezed with viral respiratory tract infections secrete higher levels of the type 1 cytokine interferon-gamma (IFN-gamma) in the peripheral circulation than children who had never wheezed. Blood was taken from 13 childr...


    pub_type: 杂志文章


    authors: Leech SC,Price JF,Holmes BJ,Kemeny DM

    更新日期:2000-01-01 00:00:00

  • The basophil activation test in wasp venom allergy: sensitivity, specificity and monitoring specific immunotherapy.

    abstract:BACKGROUND:As in vitro diagnosis of wasp venom sensitization by specific serum IgE has a sensitivity of only 60-80%, additional in vitro tests are desirable. Basophil activation is associated with the expression of CD63 and its measurement has been proposed as a novel in vitro test for immediate-type allergy. Furthermo...


    pub_type: 杂志文章


    authors: Erdmann SM,Sachs B,Kwiecien R,Moll-Slodowy S,Sauer I,Merk HF

    更新日期:2004-10-01 00:00:00

  • Inhibition of IgE binding to cross-reactive carbohydrate determinants enhances diagnostic selectivity.

    abstract:BACKGROUND:Allergy diagnosis by determination of allergen-specific IgE is complicated by clinically irrelevant IgE, of which the most prominent example is IgE against cross-reactive carbohydrate determinants (CCDs) that occur on allergens from plants and insects. Therefore, CCDs cause numerous false-positive results. I...


    pub_type: 杂志文章


    authors: Holzweber F,Svehla E,Fellner W,Dalik T,Stubler S,Hemmer W,Altmann F

    更新日期:2013-10-01 00:00:00

  • Histopathological support of antagonism of allergen-induced mast cell mediator release in human skin by a beta-2-agonist.

    abstract::The site of antagonistic action on allergen-induced early skin reactions by the beta 2-agonist terbutaline was studied by light microscopy in 10 atopic subjects. Pretreatment with 1 microgram terbutaline intradermally 5 min prior to challenge with horse dander allergen produced an approximate inhibition of 85 and 55% ...


    pub_type: 杂志文章


    authors: Grönneberg R,Lagerholm B

    更新日期:1986-01-01 00:00:00

  • Lifestyle and parental allergen sensitization are reflected in the intrauterine environment at gene expression level.

    abstract:BACKGROUND:Environmental factors, including the intrauterine environment, can influence the risk of allergy development. In the present study, we investigated whether lifestyle and parental allergen sensitization status are reflected at gene expression level in the intrauterine environment. METHODS:  mRNA expression o...


    pub_type: 杂志文章


    authors: Joerink M,Oortveld MA,Stenius F,Rindsjö E,Alm J,Scheynius A

    更新日期:2010-10-01 00:00:00

  • Chip pile workers and mould exposure. A preliminary clinical and hygienic survey.

    abstract::Chip pile workers are exposed to high concentrations of airborne mould spores. Few sporadic cases of allergic alveolitis have been reported in Finland. Eight non-smoking workers, six on chip piles and two on bark piles, were examined to study the immunological and clinical response to continuous mould spore exposure. ...


    pub_type: 杂志文章


    authors: Jäppinen P,Haahtela T,Liira J

    更新日期:1987-10-01 00:00:00

  • Interpretation of subjective symptoms in double-blind placebo-controlled food challenges - interobserver reliability.

    abstract:BACKGROUND:Subjective symptoms after food challenges are difficult to interpret and no standard is available. We discuss a strategy for how to interpret a diary. Furthermore, the interobserver reliability is evaluated. METHODS:Diaries for 32 patients with subjective symptoms were used. The diaries were re-evaluated wi...


    pub_type: 杂志文章


    authors: Gellerstedt M,Magnusson J,Gråjö U,Ahlstedt S,Bengtsson U

    更新日期:2004-03-01 00:00:00

  • Cord blood Tregs with stable FOXP3 expression are influenced by prenatal environment and associated with atopic dermatitis at the age of one year.

    abstract:BACKGROUND:Regulatory T cells (Tregs) with stable FOXP3 expression are characterized by a specific demethylated region in the FOXP3 gene (Treg-specific demethylated region, TSDR). The aim of this study was to analyse the influence of prenatal factors on cord blood Treg numbers, as detected by changes in the TSDR demeth...


    pub_type: 杂志文章


    authors: Hinz D,Bauer M,Röder S,Olek S,Huehn J,Sack U,Borte M,Simon JC,Lehmann I,Herberth G,LINA study group.

    更新日期:2012-03-01 00:00:00

  • Challenges in the implementation of EAACI guidelines on allergen immunotherapy: A global perspective on the regulation of allergen products.

    abstract::Regulatory approaches for allergen immunotherapy (AIT) products and the availability of high-quality AIT products are inherently linked to each other. While allergen products are available in many countries across the globe, their regulation is very heterogeneous. First, we describe the regulatory systems applicable f...


    pub_type: 杂志文章,评审


    authors: Bonertz A,Roberts GC,Hoefnagel M,Timon M,Slater JE,Rabin RL,Bridgewater J,Pini C,Pfaar O,Akdis C,Goldstein J,Poulsen LK,van Ree R,Rhyner C,Barber D,Palomares O,Sheikh A,Pawankar R,Hamerlijnk D,Klimek L,Agache I,

    更新日期:2018-01-01 00:00:00

  • The role of hen's egg-specific IgE, IgG and IgG4 in the diagnostic procedure of hen's egg allergy.

    abstract:BACKGROUND:Hen's egg (HE) allergy is a common disease in childhood. HE-specific serum IgE has been correlated with the outcome of oral food challenge tests, and diagnostic decision points have been described as helpful but still not sufficient to reduce the requirement for oral food challenges. The aim of the study was...


    pub_type: 杂志文章


    authors: Ahrens B,Lopes de Oliveira LC,Schulz G,Borres MP,Niggemann B,Wahn U,Beyer K

    更新日期:2010-12-01 00:00:00

  • Characterization of Der p 21, a new important allergen derived from the gut of house dust mites.

    abstract:BACKGROUND:The house dust mite (HDM) Dermatophagoides pteronyssinus is a major allergen source eliciting allergic asthma. The aim of the study was to identify new important HDM allergens associated with allergic asthma. METHODS:A cDNA coding for a new mite allergen, designated Der p 21, was isolated using immunoglobul...


    pub_type: 杂志文章


    authors: Weghofer M,Dall'Antonia Y,Grote M,Stöcklinger A,Kneidinger M,Balic N,Krauth MT,Fernández-Caldas E,Thomas WR,van Hage M,Vieths S,Spitzauer S,Horak F,Svergun DI,Konarev PV,Valent P,Thalhamer J,Keller W,Valenta R,Vrtal

    更新日期:2008-06-01 00:00:00

  • A population-based epidemiologic analysis of deaths from anaphylaxis in Florida.

    abstract:BACKGROUND:Previous US population-based epidemiologic studies of anaphylactic deaths have been limited by small populations and/or few deaths. The objective of this study was to determine the 10-year incidence of death from anaphylaxis in Florida and its descriptive epidemiology. METHODS:Patients who died from anaphyl...


    pub_type: 杂志文章


    authors: Simon MR,Mulla ZD

    更新日期:2008-08-01 00:00:00

  • Effects of interleukin (IL)-3 and IL-5 on human eosinophil degranulation induced by complement components C3a and C5a.

    abstract::It is suggested that eosinophils (Eos) play an important role in the pathogenesis of bronchial asthma by releasing cytotoxic cationic eosinophil granule proteins and damaging bronchial epithelial cells. However, the exact nature of the actual inducer of eosinophil degranulation in vivo is unclear. We examined eosinoph...


    pub_type: 杂志文章


    authors: Takafuji S,Tadokoro K,Ito K

    更新日期:1996-08-01 00:00:00

  • Selective blockade of NF-kappaB by novel mutated IkappaBalpha suppresses CD3/CD28-induced activation of memory CD4+ T cells in asthma.

    abstract:BACKGROUND:Nuclear factor kappaB (NF-kappaB) overactivation plays a crucial role in T-helper 2 (Th2)-biased allergic airway inflammation by increased activation and decreased apoptosis of CD4(+) T cells. We have shown that targeted NF-kappaB suppression in dendritic cells by adenoviral gene transfer of a novel mutated ...


    pub_type: 杂志文章


    authors: Zhou LF,Zhang MS,Hu AH,Zhu Z,Yin KS

    更新日期:2008-05-01 00:00:00

  • Nature and function of gastrointestinal antigen-presenting cells.

    abstract::Antigen-presenting cells (APCs) of the human gut are heterogeneous, including both macrophages, a variety of dendritic cells and B cells. They are found both in gut-associated lymphoid tissue and in the mucosal lamina propria, especially beneath the surface epithelium. APCs have diverse phenotypes and therefore probab...


    pub_type: 杂志文章,评审


    authors: Brandtzaeg P

    更新日期:2001-01-01 00:00:00

  • Glutaraldehyde: an occupational hazard in the hospital setting.

    abstract:BACKGROUND:We report a series of 24 health-care workers with respiratory symptoms suggestive of occupational asthma due to glutaraldehyde exposure. METHODS:The history of asthmatic symptoms was investigated with peak expiratory flow rate (PEFR) monitoring, and in eight of the subjects, the specific bronchial provocati...


    pub_type: 杂志文章


    authors: Di Stefano F,Siriruttanapruk S,McCoach J,Burge PS

    更新日期:1999-10-01 00:00:00

  • The role of house dust mite elimination in the management of childhood asthma: an unresolved issue.

    abstract::Indoor allergens are likely to be direct environmental causes of asthma and mite exposure, and sensitization is the most important environmental risk factor for childhood asthma in temperate zones. Analagous to occupational asthma, allergen avoidance in asthmatic children sensitized and exposed to mite allergens is as...


    pub_type: 杂志文章,评审


    authors: Boner A,Pescollderungg L,Silverman M

    更新日期:2002-01-01 00:00:00

  • The influence of air conditioning, humidity, temperature and other household characteristics on mite allergen concentrations in the northeastern United States.

    abstract:BACKGROUND:Information about the influence of housing and occupant characteristics on mite allergen concentrations is crucial to determine which methods could be used to decrease exposure of susceptible subjects. OBJECTIVES:To identify housing and occupant characteristics that are associated with mite allergen concent...


    pub_type: 杂志文章


    authors: van Strien RT,Gehring U,Belanger K,Triche E,Gent J,Bracken MB,Leaderer BP

    更新日期:2004-06-01 00:00:00

  • Increased prevalence of atopy among children exposed to mold in a school building.

    abstract:BACKGROUND:The purpose of this study was to assess the occurrence of immunoglobulin E sensitization to common environmental allergens (atopy) and new allergic diseases among schoolchildren after starting school in a water-damaged school building. The staff and pupils of a Finnish elementary school with visible water da...


    pub_type: 杂志文章


    authors: Savilahti R,Uitti J,Roto P,Laippala P,Husman T

    更新日期:2001-02-01 00:00:00

  • IL-33 is a mediator rather than a trigger of the acute allergic response in humans.

    abstract:BACKGROUND:IL-33 enhances FcεRI-induced mediator release in human basophils without inducing degranulation itself. In contrast, studies in mice suggested that in the presence of high IgE levels, IL-33 triggers degranulation and anaphylaxis of similar severity as specific allergen. Consistent with this view, sera of ato...


    pub_type: 杂志文章


    authors: Fux M,Pecaric-Petkovic T,Odermatt A,Hausmann OV,Lorentz A,Bischoff SC,Virchow JC,Dahinden CA

    更新日期:2014-02-01 00:00:00

  • Storage mite allergy among bakers.

    abstract::Allergy to storage mites has been shown among farmers and grain elevator workers. This study aimed to examine whether bakers also are sensitized to storage mites. Twenty-three bakers or other employees in bakeries suffering from nasal or pulmonary symptoms were compared with 17 control persons with no relation to bake...


    pub_type: 杂志文章


    authors: Revsbech P,Dueholm M

    更新日期:1990-04-01 00:00:00

  • Small-bowel capsule endoscopy in patients with gastrointestinal food allergy.

    abstract:BACKGROUND:Food allergy may present with a plethora of gastrointestinal and extraintestinal symptoms such as abdominal pain, diarrhea, cardiocirculatory symptoms, cutaneous reactions, or rhinitis. Macropathological lesions like lymphofollicular hyperplasia and erosive or ulcerative lesions have seldom been described in...


    pub_type: 杂志文章,随机对照试验


    authors: Hagel AF,de Rossi TM,Zopf Y,Lindner AS,Dauth W,Neurath MF,Raithel M

    更新日期:2012-02-01 00:00:00

  • Understanding the challenges faced by adolescents and young adults with allergic conditions: A systematic review.

    abstract:BACKGROUND:Adolescence represents a vulnerable time for individuals with asthma and allergic conditions. They suffer an unexpected degree of morbidity. This systematic review aimed to understand the challenges faced by adolescents and young adults with these conditions. METHODS:A systematic literature search was under...


    pub_type: 杂志文章


    authors: Vazquez-Ortiz M,Angier E,Blumchen K,Comberiati P,Duca B,DunnGalvin A,Gore C,Hox V,Jensen B,Pite H,Santos AF,Sanchez S,Alviani C,Garriga-Baraut T,Knibb R,Mortz CG,Gowland MH,Timmermans F,Roberts G

    更新日期:2020-08-01 00:00:00

  • Nasal inhalation of budesonide from a spacer in children with perennial rhinitis and asthma.

    abstract::The standard treatment of allergic rhinitis and asthma consists of topical corticosteroids administered intranasally and inhaled through the mouth. Although this therapy is highly effective, and side-effects are few and mild, it may be possible further to improve the therapeutic index and patient compliance with the t...


    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Pedersen W,Hjuler I,Bisgaard H,Mygind N

    更新日期:1998-04-01 00:00:00

  • Microenvironmental influences on inflammatory cell differentiation.

    abstract::Airways inflammation involves accumulation of inflammatory cells such as eosinophils, basophils and mast cells, which are derived from progenitors in marrow and blood. The inflamed tissue of the airways, through its structural (epithelium, stroma) and inflammatory cell components, produces an array of cytokines which ...


    pub_type: 杂志文章,评审


    authors: Denburg JA

    更新日期:1995-01-01 00:00:00

  • Naturally occurring human IgA autoantibodies against IgE-DES myeloma protein. Prevalence and specificity.

    abstract::The prevalence and specificity of naturally occurring human IgA anti-IgE autoantibodies (a-E Ab) were studied by ELISA with anti-IgA monoclonal antibodies (mAb) and a purified myeloma IgE as solid-phase protein, i.e., IgE-DES(kappa). Such detected IgA a-E Ab were common among adults, and significantly increased geomet...


    pub_type: 杂志文章


    authors: Magnusson CG

    更新日期:1994-12-01 00:00:00

  • A potential new target for asthma therapy: a disintegrin and metalloprotease 10 (ADAM10) involvement in murine experimental asthma.

    abstract:BACKGROUND:Elevated levels of CD23, a natural regulator of IgE production, have been shown to decrease the signs of lung inflammation in mice. The aim of this study was to study the involvement of ADAM10, the primary CD23 sheddase, in experimental asthma. METHODS:ADAM10 was blocked either by using mice with a B-cell-s...


    pub_type: 杂志文章


    authors: Mathews JA,Ford J,Norton S,Kang D,Dellinger A,Gibb DR,Ford AQ,Massay H,Kepley CL,Scherle P,Keegan AD,Conrad DH

    更新日期:2011-09-01 00:00:00

  • Occupational asthma caused by exposure to cyanoacrylate.

    abstract:BACKGROUND:Exposure to acrylates may give rise to rhinitis and asthma in both industrial and domestic environments. The mechanisms underlying these respiratory conditions caused by acrylates remain largely unknown. METHODS:We studied two assembly operators exposed to cyanoacrylate glue who developed rhinitis and asthm...


    pub_type: 杂志文章


    authors: Quirce S,Baeza ML,Tornero P,Blasco A,Barranco R,Sastre J

    更新日期:2001-05-01 00:00:00

  • Allergic respiratory disease: Different allergens, different symptoms.

    abstract:BACKGROUND:Spanish population is rather homogeneous in its genetic and sociocultural characteristics, but allergen sensitization shows wide geographical variations. We aimed at assessing whether sensitization to different allergens in the diverse geographical areas induced different clinical and quality-of-life charact...


    pub_type: 杂志文章,多中心研究


    authors: Valero A,Quirce S,Dávila I,Delgado J,Domínguez-Ortega J

    更新日期:2017-09-01 00:00:00