Increased expression of inducible cyclooxygenase-2 in human endothelial cells by antiphospholipid antibodies.

Abstract:

:The effect of IgGs from 4 patients with antiphospholipid antibodies and elevated excretion of urinary 11-dehydro-thromboxane B2 was evaluated on the production of prostacyclin by human endothelial cells in culture. After 6 h incubation, there was no change in 6-keto-prostaglandin F1 alpha in the supernatant. However patients' IgGs induced a marked increase in cyclooxygenase (Cox) activity compared to IgGs from 2 normal individuals or a commercial pool of IgGs from normal donors, tested by adding exogenous arachidonic acid. Western blot analysis of the cellular Cox content using antibodies specific for the different forms of the enzymes revealed that patients' IgGs stimulated the synthesis of the newly described inducible Cox-2 without affecting the constitutive Cox-1. This effect was partially neutralized by preincubating the IgGs with phospholipids. The induction was dependent on the amount of IgGs; it was visible at 2 h and persisted up to 24 h. Analysis of mRNA levels showed a pattern of variation in good agreement with the results obtained for protein. The protein kinase inhibitor H-7 or long-term incubation of cells with PMA strongly reduced the induction. These results suggest that antiphospholipid antibodies may not prevent the potential of the vascular cells from generating higher amounts of prostacyclin in response to acute episodes of thrombosis.

journal_name

Thromb Haemost

authors

Habib A,Martinuzzo ME,Carreras LO,Lévy-Toledano S,Maclouf J

subject

Has Abstract

pub_date

1995-08-01 00:00:00

pages

770-7

issue

2

eissn

0340-6245

issn

2567-689X

journal_volume

74

pub_type

杂志文章
  • The subendothelium of the HMEC-1 cell line supports thrombus formation in the absence of von Willebrand factor and collagen types I, III and VI.

    abstract::The macromolecular composition of the extracellular matrix (ECM) produced by the human microvascular endothelial cell line (HMEC-1) was determined by ELISA and its thrombogenicity was studied in blood perfusion assays. Results were compared with those obtained with the ECM produced by human umbilical vein endothelial ...

    journal_title:Thrombosis and haemostasis

    pub_type: 杂志文章

    doi:

    authors: Bonnefoy A,Harsfalvi J,Pfliegler G,Fauvel-Lafève F,Legrand C

    更新日期:2001-03-01 00:00:00

  • Fibrinogen gamma gene 3'-end polymorphisms and risk of venous thromboembolism in the African-American and Caucasian population.

    abstract::Genetic determinants of venous thromboembolism (VTE) in the African-American population are poorly characterised. It was recently shown that fibrinogen gamma gene (FGG) polymorphisms 10034C>T and 9340T>C influence VTE risk in the Caucasian population. In the African-American population these polymorphisms are common, ...

    journal_title:Thrombosis and haemostasis

    pub_type: 杂志文章

    doi:

    authors: Uitte de Willige S,Pyle ME,Vos HL,de Visser MC,Lally C,Dowling NF,Hooper WC,Bertina RM,Austin H

    更新日期:2009-06-01 00:00:00

  • Platelet-derived microparticles and coagulation activation in breast cancer patients.

    abstract::In the mid 1800s Trousseau observed cancer-associated thrombosis, of which the underlying pathogenesis still remains unknown. We performed a prospective study on platelet-derived microparticles (PMP) and their procoagulant potential in breast cancer patients. Fifty-eight breast cancer patients and 13 women with benign...

    journal_title:Thrombosis and haemostasis

    pub_type: 杂志文章

    doi:

    authors: Toth B,Liebhardt S,Steinig K,Ditsch N,Rank A,Bauerfeind I,Spannagl M,Friese K,Reininger AJ

    更新日期:2008-10-01 00:00:00

  • Measurement of tissue factor messenger RNA levels in human endothelial cells by a quantitative RT-PCR assay.

    abstract::We have developed a sensitive and quantitative RT-PCR assay for the determination of tissue factor (TF) mRNA levels in human cells. An in vitro synthesized internal standard RNA was used to correct for differences in reverse transcription or amplification of various RNA samples. The PCR products were quantitated by hy...

    journal_title:Thrombosis and haemostasis

    pub_type: 杂志文章

    doi:

    authors: Pötgens AJ,Lubsen NH,van Altena G,Schoenmakers JG,Ruiter DJ,de Waal RM

    更新日期:1994-02-01 00:00:00

  • Lysophosphatidylcholine decreases the synthesis of tissue factor pathway inhibitor in human umbilical vein endothelial cells.

    abstract::Thrombotic complications are frequently associated with atherosclerosis. Lysophosphatidylcholine (LPC), a component accumulated in oxidatively modified LDL (ox-LDL), is known to play a crucial role in the initiation and progression of atherosclerotic vascular lesions. Since a vascular anticoagulant, tissue factor path...

    journal_title:Thrombosis and haemostasis

    pub_type: 杂志文章

    doi:

    authors: Sato N,Kokame K,Miyata T,Kato H

    更新日期:1998-01-01 00:00:00

  • An association between oxygen desaturation and D-dimer in patients with obstructive sleep apnea syndrome.

    abstract::D-dimer, a degradation product of fibrin, is being increasingly used as a marker and prognostic factor in various thrombotic diseases. Previous reports have shown that obstructive sleep apnea is associated with platelet activation and hypercoagulability. The aim of the study was to assess the potential role of the pla...

    journal_title:Thrombosis and haemostasis

    pub_type: 杂志文章

    doi:

    authors: Shitrit D,Peled N,Shitrit AB,Meidan S,Bendayan D,Sahar G,Kramer MR

    更新日期:2005-09-01 00:00:00

  • Coagulation and fibrinolytic profile of paediatric patients undergoing cardiopulmonary bypass.

    abstract::The haemostatic system and the use of heparin during cardiopulmonary bypass (CPB) have been studied extensively in adults but not in children. Results from adult trials cannot be extrapolated to children because of age-dependent physiologic differences in haemostasis. We studied 22 consecutive paediatric patients who ...

    journal_title:Thrombosis and haemostasis

    pub_type: 杂志文章

    doi:

    authors: Chan AK,Leaker M,Burrows FA,Williams WG,Gruenwald CE,Whyte L,Adams M,Brooker LA,Adams H,Mitchell L,Andrew M

    更新日期:1997-02-01 00:00:00

  • Evidence for glycoprotein abnormality in platelets from patients with May-Hegglin anomaly.

    abstract::In the present research protein analysis on SDS-polyacrylamide gel electrophoresis (PAGE) has been used to study the glycoprotein pattern of the blood platelets of four members from a family affected by May-Hegglin anomaly. In order to characterize the glycoprotein fractions, lactoperoxidase-iodination and immunopreci...

    journal_title:Thrombosis and haemostasis

    pub_type: 杂志文章

    doi:

    authors: Ricci G,Manservigi R,Albonici L,Zavagli G,Cassai E

    更新日期:1985-12-17 00:00:00

  • Efficacy of Rivaroxaban for thromboprophylaxis after Knee Arthroscopy (ERIKA). A phase II, multicentre, double-blind, placebo-controlled randomised study.

    abstract::Without thromboprophylaxis, knee arthroscopy (KA) carries a low to moderate risk of venous thromboembolism. Over 5 million arthroscopies are performed worldwide yearly. It was our study objective to assess the efficacy and safety of rivaroxaban for thromboprophylaxis after therapeutic KA. Patients undergoing KA in nin...

    journal_title:Thrombosis and haemostasis

    pub_type: 杂志文章,多中心研究,随机对照试验

    doi:10.1160/TH16-02-0118

    authors: Camporese G,Bernardi E,Noventa F,Bosco M,Monteleone G,Santoro L,Bortoluzzi C,Freguja S,Nardin M,Marullo M,Zanon G,Mazzola C,Damiani G,Maniscalco P,Imberti D,Lodigiani C,Becattini C,Tonello C,Agnelli G,ERIKA Study Gr

    更新日期:2016-08-01 00:00:00

  • Prophylaxis in people with haemophilia.

    abstract::A four-decade clinical experience and recent evidence from randomised controlled studies definitively recognised primary prophylaxis, i.e. the regular infusion of factor concentrates started after the first haemarthrosis and/or before the age of two years, as the first-choice treatment in children with severe haemophi...

    journal_title:Thrombosis and haemostasis

    pub_type: 杂志文章,评审

    doi:

    authors: Coppola A,Franchini M,Tagliaferri A

    更新日期:2009-04-01 00:00:00

  • Development of prognostic tools in pulmonary arterial hypertension: lessons from modern day registries.

    abstract::Pulmonary arterial hypertension (PAH) is characterised by increased pressure in the pulmonary arteries leading to right-sided ventricular failure, and death. Identification of factors that affect patient survival is important to improve patient management and outcomes. The first registry to evaluate survival and devel...

    journal_title:Thrombosis and haemostasis

    pub_type: 杂志文章,评审

    doi:10.1160/TH11-11-0821

    authors: Benza RL,Gomberg-Maitland M,Frost AE,Frantz RP,Humbert M,McGoon MD

    更新日期:2012-12-01 00:00:00

  • Thrombin-Induced and Collagen-Induced Platelet Aggregation.

    abstract:: ...

    journal_title:Thrombosis and haemostasis

    pub_type: 杂志文章

    doi:10.1055/s-0038-1657077

    authors: Botney MJ,Swanson AL,Shuman MN

    更新日期:1979-07-01 00:00:00

  • Safety and tolerability of an immediate-release formulation of theoral direct thrombin inhibitor AZD0837 in the prevention of stroke and systemic embolism in patients with atrial fibrillation.

    abstract::AZD0837 is an investigational oral anticoagulant which is converted to the active form, AR-H067637, a selective direct thrombin inhibitor. The present study, a multicentre, randomised, parallel-group, dose-guiding study, assessed the safety and tolerability of an immediate-release formulation of AZD0837 compared with ...

    journal_title:Thrombosis and haemostasis

    pub_type: 杂志文章,多中心研究,随机对照试验

    doi:10.1160/TH09-07-0509

    authors: Olsson SB,Rasmussen LH,Tveit A,Jensen E,Wessman P,Panfilov S,Wåhlander K

    更新日期:2010-03-01 00:00:00

  • Increased level of oxidized LDL-dependent monocyte-derived microparticles in acute coronary syndrome.

    abstract::We measured and compared the levels of plasma soluble (s) P-selectin, sCD40L, platelet-derived microparticles (PDMP), monocyte-derived microparticles (MDMP), and anti-oxidized LDL antibody, to obtain a better understanding of their potential contribution to vascular complications in acute coronary syndrome (ACS). The ...

    journal_title:Thrombosis and haemostasis

    pub_type: 杂志文章

    doi:10.1160/TH03-04-0247

    authors: Matsumoto N,Nomura S,Kamihata H,Kimura Y,Iwasaka T

    更新日期:2004-01-01 00:00:00

  • Circulating and platelet-derived microparticles in human blood enhance thrombosis on atherosclerotic plaques.

    abstract::Plaque rupture followed by thrombosis is the underlying cause of the majority of acute coronary syndromes. Circulating microparticles (cMPs), membrane blebs released into blood by activated cells, have been associated to vascular diseases. Specifically, high levels of platelet-derived microparticles (pMPs) have been f...

    journal_title:Thrombosis and haemostasis

    pub_type: 杂志文章

    doi:10.1160/TH12-07-0486

    authors: Suades R,Padró T,Vilahur G,Badimon L

    更新日期:2012-12-01 00:00:00

  • Clinical utility of prothrombin fragment 1+2, thrombin antithrombin III complexes and D-dimer measurements in the diagnosis of deep vein thrombosis following total hip replacement.

    abstract:BACKGROUND:Measurements of prothrombin fragment 1+2 (F1+2), thrombin antithrombin III complexes (TAT) and D-dimer plasma levels have been proposed as non-invasive screening tests to exclude postoperative deep venous thrombosis (DVT). We investigated the diagnostic efficacy of these coagulation activation markers to rul...

    journal_title:Thrombosis and haemostasis

    pub_type: 临床试验,杂志文章,多中心研究,随机对照试验

    doi:

    authors: Cofrancesco E,Cortellaro M,Corradi A,Ravasi F,Bertocchi F

    更新日期:1998-03-01 00:00:00

  • Dermatan sulphate for the prevention of postoperative venous thromboembolism in patients with cancer. DOS (Dermatan sulphate in Oncologic Surgery) Study Group.

    abstract:BACKGROUND:Patients undergoing surgery for cancer are at high risk for venous thromboembolism (VTE). Agents that selectively inhibit thrombin, such as dermatan sulphate, have potential for a favourable benefit-risk ratio in the prevention of this complication. METHODS:Patients scheduled for elective abdominal, thoraci...

    journal_title:Thrombosis and haemostasis

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:

    authors: Di Carlo V,Agnelli G,Prandoni P,Coccheri S,Gensini GF,Gianese F,Mannucci PM

    更新日期:1999-07-01 00:00:00

  • Collaborative study on assays of activated FIX (FIXa). On behalf of the factor VIII and factor IX subcommittee of the ISTH. International Society on Thrombosis and Haemostasis.

    abstract::A collaborative study has been organised by NIBSC to examine the performance of FIXa assays between laboratories, and to investigate the need for a standard. Ampoules of 3 materials, one monocomponent concentrate (coded C) and 2 different preparations of purified human FIXa (one proposed reference preparation, coded A...

    journal_title:Thrombosis and haemostasis

    pub_type: 杂志文章

    doi:

    authors: Gray E,Walker D,Heath A,Barrowcliffe TW

    更新日期:1996-12-01 00:00:00

  • ADP-mimicking platelet aggregation caused by rugosin E, an ellagitannin isolated from Rosa rugosa Thunb.

    abstract::Among the nine ellagitannins, rugosin E was the most potent platelet aggregating agent with an EC50 of 1.5 +/- 0.1 microM in rabbit platelets and 3.2 +/- 0.1 microM in human platelets. The aggregations caused by rugosin E and ADP were inhibited by EGTA, PGE1, mepacrine, sodium nitroprusside and neomycin, but not by in...

    journal_title:Thrombosis and haemostasis

    pub_type: 杂志文章

    doi:

    authors: Teng CM,Kang YF,Chang YL,Ko FN,Yang SC,Hsu FL

    更新日期:1997-03-01 00:00:00

  • Three abnormal fibrinogen variants with the same amino acid substitution (gamma 275 Arg----His): fibrinogens Bergamo II, Essen and Perugia.

    abstract::We report on three unrelated individuals with the same uncommon type of dysfibrinogenemia, originating from Bergamo, Essen and Perugia. None of them showed bleeding symptoms while the Bergamo patient and members of her family presented with a thrombotic tendency. The presence of a defective fibrinogen was suggested by...

    journal_title:Thrombosis and haemostasis

    pub_type: 杂志文章

    doi:

    authors: Reber P,Furlan M,Henschen A,Kaudewitz H,Barbui T,Hilgard P,Nenci GG,Berrettini M,Beck EA

    更新日期:1986-12-15 00:00:00

  • Endotoxin-induced intravascular coagulation in rabbits: effect of tissue plasminogen activator vs urokinase of PAI generation, fibrin deposits and mortality.

    abstract::We have evaluated the effect of plasminogen activators (t-PA and urokinase) on an experimental model of disseminated intravascular coagulation (DIC) in rabbits by injection of 20 micrograms/kg/h of E. coli lipopolysaccharide during 6 h t-PA (0.2 mg/kg and 0.7 mg/kg), urokinase (3000 U/kg/h) and saline (control) were g...

    journal_title:Thrombosis and haemostasis

    pub_type: 杂志文章

    doi:

    authors: Paloma MJ,Páramo JA,Rocha E

    更新日期:1995-12-01 00:00:00

  • The factor V gene A4070G mutation and the risk of venous thrombosis.

    abstract::The A4070G polymorphism in exon 13 of the factor V (FV) gene, which replaces His by Arg at position 1299 of the B domain, was recently shown to influence circulating FV levels and to contribute to the activated protein C (APC) resistance phenotype. We examined the impact of this polymorphism in a population of unselec...

    journal_title:Thrombosis and haemostasis

    pub_type: 杂志文章

    doi:

    authors: Alhenc-Gelas M,Nicaud V,Gandrille S,van Dreden P,Amiral J,Aubry ML,Fiessinger JN,Emmerich J,Aiach M

    更新日期:1999-02-01 00:00:00

  • The isolation and characterization of a ternary human plasmin B-chain-streptokinase-plasminogen complex.

    abstract::A ternary equimolar human plasmin B-chain-streptokinase-plasminogen complex was isolated from a mixture of the plasmin B-chain-streptokinase complex and human plasminogen at 0 degrees C and 37 degrees C. A ternary complex which was shown to be species specific, was identified and characterized by ultracentrifugal, acr...

    journal_title:Thrombosis and haemostasis

    pub_type: 杂志文章

    doi:

    authors: Summaria L,Boreisha I,Barlow GH,Robbins KC

    更新日期:1987-08-04 00:00:00

  • Multicenter comparison of von Willebrand factor multimer sizing techniques. Report of the Factor VIII and von Willebrand Factor Subcommittee.

    abstract::A multicenter study of various types of von Willebrand's disease (vWD) was conducted in order to compare the different electrophoretic techniques used to evaluate von Willebrand factor multimers in plasma. Seven laboratories participated in the blind study of eight plasma samples from two healthy subjects and six vWD ...

    journal_title:Thrombosis and haemostasis

    pub_type: 杂志文章

    doi:

    authors: Mannucci PM,Abildgaard CF,Gralnick HR,Hill FG,Hoyer LW,Lombardi R,Nilsson IM,Tuddenham E,Meyer D

    更新日期:1985-12-17 00:00:00

  • Thrombolysis in deep vein thrombosis: is there still an indication?

    abstract::The most accepted therapy for DVT consists of anticoagulation with unfractionated heparin or low molecular weight heparin, followed by variable duration oral anticoagulation but thrombolytic therapy has been proposed in addition to standard anticoagulation. This paper reviews the literature on post thrombotic syndrome...

    journal_title:Thrombosis and haemostasis

    pub_type: 杂志文章,meta分析

    doi:

    authors: Wells PS,Forster AJ

    更新日期:2001-07-01 00:00:00

  • High on-treatment platelet reactivity--definition and measurement.

    abstract::In the last decade, several studies revealed inter-patient response variability to antiplatelet agents: patients who display negligible or no responses to these drugs are considered poor responders, or "resistant" to treatment. In order to identify poor responders to an antiplatelet drug, laboratory tests of platelet ...

    journal_title:Thrombosis and haemostasis

    pub_type: 杂志文章,评审

    doi:10.1160/TH12-10-0758

    authors: Cattaneo M

    更新日期:2013-05-01 00:00:00

  • Anti-thrombotic effects of selective estrogen receptor modulator tamoxifen.

    abstract::Tamoxifen is a known anti-cancer drug and established estrogen receptor modulator. Few clinical studies have earlier implicated the drug in thrombotic complications attributable to lower anti-thrombin and protein S levels in plasma. However, action of tamoxifen on platelet signalling machinery has not been elucidated ...

    journal_title:Thrombosis and haemostasis

    pub_type: 杂志文章

    doi:10.1160/TH11-03-0178

    authors: Nayak MK,Singh SK,Roy A,Prakash V,Kumar A,Dash D

    更新日期:2011-10-01 00:00:00

  • Rituximab for treatment of inhibitors in haemophilia A. A Phase II study.

    abstract::The development of antibodies against infused factor VIII (FVIII) in patients with haemophilia A is a serious complication leading to poorly controlled bleeding and increased morbidity. No treatment has been proven to reduce high titre antibodies in patients who fail immune tolerance induction or are not candidates fo...

    journal_title:Thrombosis and haemostasis

    pub_type: 杂志文章,多中心研究

    doi:10.1160/TH14-01-0078

    authors: Leissinger C,Josephson CD,Granger S,Konkle BA,Kruse-Jarres R,Ragni MV,Journeycake JM,Valentino L,Key NS,Gill JC,McCrae KR,Neufeld EJ,Manno C,Raffini L,Saxena K,Torres M,Marder V,Bennett CM,Assmann SF

    更新日期:2014-09-02 00:00:00

  • Platelet adhesion to native collagens involves proteoglycans and may be a two-step process.

    abstract::Adhesion of rat blood platelets to native rat tail collagen fibrils was studied in the electron microscope under conditions that preserved collagen-associated proteoglycans (CAPG). The CAPG molecules were aligned in chain-like configurations that encircled the fibrils with a 65 nm period; they appeared to coat the fib...

    journal_title:Thrombosis and haemostasis

    pub_type: 杂志文章

    doi:

    authors: Behnke O

    更新日期:1987-08-04 00:00:00

  • Inter-patient variability and impact of proton pump inhibitors on platelet reactivity after prasugrel.

    abstract::Although there is considerable variability of platelet reactivity among patients treated with clopidogrel, little is known about inter-individual differences and possible role of proton pump inhibitors (PPIs) after prasugrel. We defined the extent of inter-patient variability, and evaluated the impact of PPI interacti...

    journal_title:Thrombosis and haemostasis

    pub_type: 杂志文章,多中心研究

    doi:10.1160/TH11-09-0622

    authors: Aradi D,Kuliczkowski W,Atar D,Serebruany VL

    更新日期:2012-02-01 00:00:00