Characterisation of melatonin binding sites in the eye of the Japanese quail (Coturnix japonica).


:Melatonin binding sites were examined in the quail eye using 2-[125]iodomelatonin. Radioreceptor assays indicated similar binding sites in membrane preparations of neural retina (NR) and choroid-retinal pigment epithelium (C-RPE) eye components. In both tissues binding of the radioligand was specific, saturable, and of high affinity [Kd values NR 50.8 +/- 19.5 pM, C-RPE 98.2 +/- 35.4 pM, mean +/- SEM (n = 4)] and low capacity (Bmax values NR 12.4 +/- 2.7 fmol/mg protein, C-RPE 21.5 +/- 3.2 fmol/mg protein). Kinetic studies demonstrated that association of 2-[125I]iodomelatonin was rapid and further that this binding was reversible upon the addition of 1 microM melatonin. The order of pharmacological potencies of various indoles tested in 2-[125I]iodomelatonin displacement studies was melatonin > 6-chloromelatonin > 6-hydroxymelatonin > N-acetylserotonin > 5-methoxytryptophol > 5-hydroxytryptamine > 5-methoxytryptamine (5-hydroxytryptamine > 5-methoxytryptophol for C-RPE). Studies with guanine nucleotides indicated that the signal transduction mechanism of the binding site may involve a G-protein linkage. This melatonin binding site displays several pharmacological similarities with those investigated in the retina of other species and with those previously characterised in the quail brain.


Gen Comp Endocrinol


James K,Skene DJ,Lucini V,Stankov B,Arendt J




Has Abstract


1995-11-01 00:00:00














  • Molecular characterization of ghrelin and gastrin-releasing peptide in Atlantic cod (Gadus morhua): cloning, localization, developmental profile and role in food intake regulation.

    abstract::Full-length complementary deoxyribonucleic acid as well as genomic sequences encoding for two gastrointestinal appetite-related peptides, ghrelin and for gastrin-releasing peptide (GRP) were cloned from Atlantic cod (Gadus morhua) stomach using reverse transcription and rapid amplification of complementary deoxyribonu...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Xu M,Volkoff H

    更新日期:2009-02-01 00:00:00

  • Peripheral blood levels of progesterone in female camels during various reproductive stages.

    abstract::Determination of serum progesterone concentrations over a period of 2 years in female camels during various reproductive stages (estrus, pregnant, nonpregnant, and lactating) suggests that the progesterone levels exceeding 1 ng/ml are reached only if mating is fertile. A cycling camel does not have a luteal phase and ...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Elias E,Bedrak E,Yagil R

    更新日期:1984-02-01 00:00:00

  • Cloning, phylogenetic analysis and expression of somatolactin and its receptor in Cichlasoma dimerus: their role in long-term background color acclimation.

    abstract::Somatolactin (SL) and SL receptor (SLR) belong to the growth hormone and cytokine type I receptor superfamilies, respectively. However, further research is required to define the duplications and functions of SL and its receptors in basal vertebrates including environmental background color adaptation in fish. In the ...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Cánepa MM,Zhu Y,Fossati M,Stiller JW,Vissio PG

    更新日期:2012-03-01 00:00:00

  • Protein kinase-C mediates chicken vasoactive intestinal peptide stimulated prolactin secretion and gene expression in turkey primary pituitary cells.

    abstract::This set of experiments investigated the role of protein kinase-C (PKC) as a second messenger in vasoactive intestinal peptide (VIP)-stimulated prolactin (PRL) secretion and PRL mRNA abundance. Dispersed anterior pituitary cells (5 x 10(5) or 10(6) cells/tube) were isolated from laying turkeys and incubated in 1.0 ml ...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Sun S,el Halawani ME

    更新日期:1995-09-01 00:00:00

  • Transfer of tilapia (Oreochromis niloticus) to a hyperosmotic environment is associated with sustained expression of prolactin receptor in intestine, gill, and kidney.

    abstract::Expression of the tilapia prolactin receptor (tiPRL-R) has been characterized in the intestine of Oreochromis niloticus and the levels of both tiPRL-R transcripts and tiPRL binding sites have been further analyzed in this organ, as well as in gill and kidney, during adaptation of tilapia to a hyperosmotic environment....

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Sandra O,Le Rouzic P,Rentier-Delrue F,Prunet P

    更新日期:2001-09-01 00:00:00

  • Effects of P-nitrophenol exposure on the testicular development and semen quality of roosters.

    abstract::The widespread use of P-nitrophenol (PNP) as a raw material in pesticides, medicines and dyes has led to environmental pollution. PNP is a well-known endocrine disruptor in mammals and quails. This study investigated the effects of long-term PNP exposure on the testicular development and semen quality of roosters. Pub...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Ren S,Li Y,Li C

    更新日期:2021-01-15 00:00:00

  • No evidence for sex-specific effects of the maternal social environment on offspring development in Japanese quail (Coturnix japonica).

    abstract::The social environment of reproducing females can cause physiological changes, with consequences for reproductive investment and offspring development. These prenatal maternal effects are often found to be sex-specific and may have evolved as adaptations, maximizing fitness of male and female offspring for their futur...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Langen EMA,von Engelhardt N,Goerlich-Jansson VC

    更新日期:2018-07-01 00:00:00

  • A domestication related mutation in the thyroid stimulating hormone receptor gene (TSHR) modulates photoperiodic response and reproduction in chickens.

    abstract::The thyroid stimulating hormone receptor gene (TSHR) has been suggested to be a "domestication locus" in the chicken. A strong selective sweep over TSHR in domestic breeds together with significant effects of a mutation in the gene on several domestication related traits, indicate that the gene has been important for ...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Karlsson AC,Fallahshahroudi A,Johnsen H,Hagenblad J,Wright D,Andersson L,Jensen P

    更新日期:2016-03-01 00:00:00

  • Effects of epidermal growth factor on isolated digestive gland cells from mussels.

    abstract::Effects of epidermal growth factor (EGF) and possible mechanisms of EGF-mediated signal transduction were investigated in isolated cells of the digestive gland of the mussel (Mytilus galloprovincialis Lam. ). EGF induced a cytosolic Ca2+ transient and subsequently stimulated DNA synthesis; both effects were dose-depen...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Canesi L,Ciacci C,Orunesu M,Gallo G

    更新日期:1997-08-01 00:00:00

  • Sexual steroids in Salamandra salamandra (L.) during ontogeny.

    abstract::In order to investigate the ontogeny of steroid secretions during the juvenile phase, sexual steroids were extracted and assayed after chromatographic separation in the gonads and plasma of male and female salamanders Salamandra salamandra L. at three characteristic postlarval stages. All specimens were collected in t...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Garnier DH,Joly J

    更新日期:1991-01-01 00:00:00

  • Immunohisto- and immunocytochemical studies on the dynamics of TSH and GTH cells in normally metamorphosing, metamorphosed, and metamorphosis-arrested Hynobius retardatus.

    abstract::The development and dynamics of TSH and GTH cells were studied immunohisto- and immunocytochemically in the salamander Hynobius retardatus, which had been reported to show neoteny in a specific environment and a precocious development and maturation of testis in goitrogen-treated larvae. Pituitary glands from normally...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Yamaguchi M,Tanaka S,Wakahara M

    更新日期:1996-12-01 00:00:00

  • Delayed access to feed alters gene expression associated with hormonal signaling, cellular differentiation, and protein metabolism in muscle of newly hatch chicks.

    abstract::Birds rely solely on utilization of the yolk sac as a means of nutritional support throughout embryogenesis and early post-hatch, before first feeding occurs. Newly hatched broiler (meat-type) chickens are frequently not given immediate access to feed, and this can result in numerous alterations to developmental proce...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Payne JA,Proszkowiec-Weglarz M,Ellestad LE

    更新日期:2020-06-01 00:00:00

  • Follistatin suppresses FSHbeta but increases LHbeta expression in the goldfish - evidence for an activin-mediated autocrine/paracrine system in fish pituitary.

    abstract::Our previous study demonstrated that recombinant goldfish activin B stimulated goldfish FSHbeta but inhibited LHbeta expression. Similar to activin B, activin A also exhibited the inverse effects on the expression of the two gonadotropins. The novel dual effects of activins on FSH and LH in the goldfish raise an inter...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Yuen CW,Ge W

    更新日期:2004-01-01 00:00:00

  • Immunocytochemical and morphometric study of TSH, PRL, GH, and ACTH cells in Bufo arenarum larvae with inhibited thyroid function.

    abstract::Changes in the pituitary cells involved in amphibian metamorphosis were studied in Bufo arenarum tadpoles with inhibited thyroid function. After hatching, larvae were treated for 5 months with potassium perchlorate (KClO4), a goitrogenic substance known to prevent absorption of iodine from water or food, resulting in ...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Miranda LA,Paz DA,Dezi RE,Pisanó A

    更新日期:1995-05-01 00:00:00

  • Saturable triiodothyronine-binding sites in the pituitary nuclei of salmonid teleost fish.

    abstract::High-affinity, limited-capacity, 3,5,3'-triiodo-L-thyronine (T3)-binding sites were established by in vitro saturation analysis in cell nuclei of the pituitary gland of arctic charr. The sites were extracted from the purified nuclei using 0.4 M NaCl and incubated with [125I]T3 in the presence of 0.2 M NaCl. T3 saturab...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Bres O,Eales JG

    更新日期:1990-01-01 00:00:00

  • Effects of melatonin in connection with the antioxidant defense system in the gills of the estuarine crab Neohelice granulata.

    abstract::Numerous studies have shown that melatonin exerts some influence on the antioxidant defense system (ADS) in vertebrates, but for crustaceans no such effect has been demonstrated till now. However, earlier reports did show a similar profile of daily variations in the ADS of the gills and the melatonin content of the ey...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Maciel FE,Ramos BP,Geihs MA,Vargas MA,Cruz BP,Meyer-Rochow VB,Vakkuri O,Allodi S,Monserrat JM,Nery LE

    更新日期:2010-01-15 00:00:00

  • Hormonal daily variation co-varies with immunity in captive male bullfrogs (Lithobates catesbeianus).

    abstract::Almost all physiological processes within the organism, including immune parameters and hormones, follow a circadian rhythm. These daily fluctuations are often observed in free-living organisms; however, little is known regarding hormonal and immune daily variations in anurans, particularly under laboratory conditions...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Titon SCM,Titon Junior B,Assis VR,Vasconcelos-Teixeira R,Garcia Neto PG,Lima AS,Ferreira LF,Fernandes PA,Gomes FR,Markus RP

    更新日期:2020-12-20 00:00:00

  • Isolation, synthesis, and biological activity of flounder [Asn1,Ile5,Thr9] angiotensin I.

    abstract::A novel angiotensin I (ANG I) has been isolated from incubates of plasma and kidney extracts of the flounder, Platichthys flesus, using ion-exchange, gel-permeation, and reverse-phase high performance liquid chromatography (HPLC). Its sequence was determined as H-Asn-Arg-Val-Tyr-Ile-His-Pro-Phe-Thr-Leu-OH by sequence ...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Balment RJ,Warne JM,Takei Y

    更新日期:2003-01-01 00:00:00

  • Production of two recombinant insulin-like growth factor binding protein-1 subtypes specific to salmonids.

    abstract::Salmonids have four subtypes of insulin-like growth factor binding protein (IGFBP)-1, termed -1a1, -1a2, -1b1 and 1b2, owing to teleost- and a lineage-specific whole-genome duplications. We have previously produced recombinant proteins of masu salmon IGFBP-1a1 and -1b2 and conducted functional analysis. To further cha...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Hasegawa R,Miura T,Kaneko N,Kizaki R,Oishi G,Tanaka H,Sato M,Shimizu M

    更新日期:2020-12-01 00:00:00

  • A peak in gh-receptor expression is associated with growth activation in Atlantic salmon vertebrae, while upregulation of igf-I receptor expression is related to increased bone density.

    abstract::Growth hormone (GH) and insulin-like growth factor-I (IGF-I) play major roles in the endocrine regulation of fish growth, but their interdependency and mode of action has not been well elucidated. The GH-IGF-I system is essential for normal vertebral growth in mouse, but this has not been studied in fish. To study the...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Wargelius A,Fjelldal PG,Benedet S,Hansen T,Björnsson BT,Nordgarden U

    更新日期:2005-05-15 00:00:00

  • Glucagon- and NPY-related peptide-immunoreactive cells in the gut of sea bass (Dicentrarchus labrax L.): a light and electron microscopic study.

    abstract::Glucagon and peptide of the neuropeptide Y (NPY) family immunoreactivities were studied in the gut of sea bass (Dicentrarchus labrax) using antisera against bovine/porcine glucagon, porcine glucagon, glicentin (10-30), bovine pancreatic polypeptide (PP), peptide tyrosine tyrosine (PYY), salmon PYY (sPYY), and NPY. Glu...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Gómez-visus I,García-Hernández MP,Lozano MT,Agulleiro B

    更新日期:1998-10-01 00:00:00

  • Real-time PCR analysis of cytochrome P450 aromatase expression in zebrafish: gene specific tissue distribution, sex differences, developmental programming, and estrogen regulation.

    abstract::In teleost fish, the predominant brain form of cytochrome P450 aromatase (P450aromB) is a neural marker of estrogen effect, and an entry point for studying the role of hormonal and environmental estrogens on neurodevelopment and neuroplasticity. As part of a project using zebrafish to investigate these issues, we deve...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Sawyer SJ,Gerstner KA,Callard GV

    更新日期:2006-06-01 00:00:00

  • Morphometric analysis of small follicles in the ovary of the musk shrew (Suncus murinus L.) at different ages.

    abstract::On the basis of morphological characteristics of granulosa cells surrounding the oocyte, the small follicles (Class 1) in the ovary of the musk shrew were classified into four groups: Type B (flattened cells), Type B/C (mixture of flattened and cuboidal cells), Type C (single layer of cuboidal cells), and Type D (more...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Mohanty N,Chainy GB

    更新日期:1992-02-01 00:00:00

  • Somatotropic axis genes are expressed before pituitary onset during zebrafish and sea bass development.

    abstract::The somatotropic axis, or growth hormone-insulin-like growth factor-1 (GH-IGF-1) axis, of fish is involved in numerous physiological process including regulation of ionic and osmotic balance, lipid, carbohydrate and protein metabolism, growth, reproduction, immune function and behavior. It is thought that GH plays a r...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Besseau L,Fuentès M,Sauzet S,Beauchaud M,Chatain B,Covès D,Boeuf G,Falcón J

    更新日期:2013-12-01 00:00:00

  • Expression of estrogen receptors in female rainbow trout (Oncorhynchus mykiss) during first ovarian development and under dense rearing condition.

    abstract::To study the expression of four estrogen receptor genes (erα1, erα2, erβ1, erβ2) of female rainbow trout (Oncorhynchus mykiss) during first ovarian development, trouts were sampled from different ovarian stages. Serum E2 (estradiol) was measured by ELISA and estrogen receptors mRNA expression were examined by qRT-PCR....

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Hou ZS,Wen HS,Li JF,He F,Li Y,Tao YX

    更新日期:2018-04-01 00:00:00

  • Molecular evolution of adiponectin in Carnivora and its mRNA expression in relation to hepatic lipidosis.

    abstract::Adiponectin is a novel adipocyte-derived hormone with low circulating concentrations and/or mRNA expression in obesity and non-alcoholic fatty liver disease (NAFLD). The adiponectin mRNA of several Carnivora species was sequenced to enable further gene expression studies in this clade with potential experimental speci...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Nieminen P,Rouvinen-Watt K,Kapiainen S,Harris L,Mustonen AM

    更新日期:2010-09-15 00:00:00

  • Effects of vertebrate prolactins and growth hormones on thyroxine 5'-monodeiodination in the eel (Anguilla anguilla): a potential bioassay for growth hormone.

    abstract::Growth hormones (GHs) and prolactins (Prls) purified from representatives of each vertebrate class from bony fish onwards were tested for their ability to stimulate in vivo peripheral deiodination of labeled thyroxine (T4*) into triiodo-L-thyronine (T3*) in the eel. Plasma T3*/T4* ratio was used as parameter. All GHs ...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: de Luze A,Leloup J,Papkoff H,Kikuyama S,Kawauchi H

    更新日期:1989-02-01 00:00:00

  • An insulin-like growth factor found in hepatopancreas implicates carbohydrate metabolism of the blue crab Callinectes sapidus.

    abstract::Hyperglycemia that is caused by the release of crustacean hyperglycemic hormone (CHH) from the sinus gland to hemolymph is one of the hallmark physiological phenomena, occurring in decapod crustaceans experiencing stressful conditions. However, the mechanism(s) by which such elevated glucose levels return to resting l...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Chung JS

    更新日期:2014-04-01 00:00:00

  • Seasonal changes in androgen receptor mRNA in the brain of the white-crowned sparrow.

    abstract::In songbirds, neurons that regulate learned song behavior undergo extensive seasonal plasticity in their number and size in relation to the bird's reproductive status. Seasonal plasticity of these brain regions is primarily regulated by changes in circulating concentrations of testosterone. Androgen receptors are pres...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Fraley GS,Steiner RA,Lent KL,Brenowitz EA

    更新日期:2010-03-01 00:00:00

  • Rearing conditions have long-term consequences for stress responsiveness in free-living great tits.

    abstract::In captivity, the adrenocortical stress response can be permanently altered by events that occur during early life. Free-living animals have rarely been examined in this regard. To examine whether early-life events impact the hypothalamo-pituitary-adrenal (HPA) axis in the natural setting, we evaluated the stress resp...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Landys MM,Goymann W,Slagsvold T

    更新日期:2011-11-01 00:00:00