Characterisation of melatonin binding sites in the eye of the Japanese quail (Coturnix japonica).


:Melatonin binding sites were examined in the quail eye using 2-[125]iodomelatonin. Radioreceptor assays indicated similar binding sites in membrane preparations of neural retina (NR) and choroid-retinal pigment epithelium (C-RPE) eye components. In both tissues binding of the radioligand was specific, saturable, and of high affinity [Kd values NR 50.8 +/- 19.5 pM, C-RPE 98.2 +/- 35.4 pM, mean +/- SEM (n = 4)] and low capacity (Bmax values NR 12.4 +/- 2.7 fmol/mg protein, C-RPE 21.5 +/- 3.2 fmol/mg protein). Kinetic studies demonstrated that association of 2-[125I]iodomelatonin was rapid and further that this binding was reversible upon the addition of 1 microM melatonin. The order of pharmacological potencies of various indoles tested in 2-[125I]iodomelatonin displacement studies was melatonin > 6-chloromelatonin > 6-hydroxymelatonin > N-acetylserotonin > 5-methoxytryptophol > 5-hydroxytryptamine > 5-methoxytryptamine (5-hydroxytryptamine > 5-methoxytryptophol for C-RPE). Studies with guanine nucleotides indicated that the signal transduction mechanism of the binding site may involve a G-protein linkage. This melatonin binding site displays several pharmacological similarities with those investigated in the retina of other species and with those previously characterised in the quail brain.


Gen Comp Endocrinol


James K,Skene DJ,Lucini V,Stankov B,Arendt J




Has Abstract


1995-11-01 00:00:00














  • Opiate inhibition of growth hormone secretion in young chickens.

    abstract::Plasma concentrations of growth hormone (GH) were decreased following the intravenous administration of morphine sulfate. Maximum inhibition of GH secretion was observed 40 min after morphine sulfate challenge. At this time, doses of morphine sulfate (at 5 mg and 50 mg/kg) reduced the GH concentrations by 86 and 90%, ...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Harvey S,Scanes CG

    更新日期:1987-01-01 00:00:00

  • Hormonal daily variation co-varies with immunity in captive male bullfrogs (Lithobates catesbeianus).

    abstract::Almost all physiological processes within the organism, including immune parameters and hormones, follow a circadian rhythm. These daily fluctuations are often observed in free-living organisms; however, little is known regarding hormonal and immune daily variations in anurans, particularly under laboratory conditions...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Titon SCM,Titon Junior B,Assis VR,Vasconcelos-Teixeira R,Garcia Neto PG,Lima AS,Ferreira LF,Fernandes PA,Gomes FR,Markus RP

    更新日期:2020-12-20 00:00:00

  • Thyroidectomy of house sparrows (Passer domesticus) prevents photo-induced testicular growth but not the increased hypothalamic gonadotrophin-releasing hormone.

    abstract::Thyroidectomy of starlings (Sturnus vulgaris) prevents the decrease in hypothalamic gonadotrophin-releasing hormone (GnRH) which normally occurs at the onset of photorefractoriness. To extend this observation to another species, changes in testicular mass, bill colour, moult, and hypothalamic GnRH content were monitor...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Dawson A

    更新日期:1998-05-01 00:00:00

  • The maternal control in the embryonic development of zebrafish.

    abstract::The maternal control directing the very first hours of life is of pivotal importance for ensuring proper development to the growing embryo. Thanks to the finely regulated inheritance of maternal factors including mRNAs and proteins produced during oogenesis and stored into the mature oocyte, the embryo is sustained th...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Miccoli A,Dalla Valle L,Carnevali O

    更新日期:2017-05-01 00:00:00

  • Effects of developmental conditions on nestling American Kestrel (Falco sparverius) corticosterone concentrations.

    abstract::How nestling birds respond to stressful situations may constitute an important survival component that has lasting developmental effects on the hypothalamic pituitary adrenal (HPA) axis. As birds are exposed to increasing amounts of potential anthropogenic stressors through land use change, understanding how these fac...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Strasser EH,Heath JA

    更新日期:2011-08-01 00:00:00

  • Hormonal regulation of the epithelial Na+ channel: from amphibians to mammals.

    abstract::High-resistance epithelia derived from amphibian sources such as frog skin, toad urinary bladder, and the A6 Xenopus laevis kidney cell line have been widely used to elucidate the underlying mechanisms involved in the regulation of vectorial ion transport. More recently, the isolation of high-resistance mammalian cell...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Shane MA,Nofziger C,Blazer-Yost BL

    更新日期:2006-05-15 00:00:00

  • Vasoactive intestinal peptide is a hypothalamic prolactin-releasing neuropeptide in the turkey (Meleagris gallopavo).

    abstract::The hypothesis that vasoactive intestinal peptide (VIP) functions as a hypothalamic prolactin (PRL)-releasing peptide in the turkey was tested by determining the effects of hypothalamic VIP immunoneutralization and pituitary VIP receptor blockade on hypothalamic extract (HE)-induced PRL secretion from dispersed anteri...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: el Halawani ME,Silsby JL,Mauro LJ

    更新日期:1990-04-01 00:00:00

  • Thyroid and annual gonad development, body weight, plumage pigmentation, and bill color cycles of lal munia, Estrilda amandava.

    abstract::Long-term administration of L-thyroxine (T4) in the wax bill (lal munia) inhibited the current gonad development cycle, and the increase in gonadal volume in the second cycle was suppressed only by higher concentrations. Gonadal inhibition by high but not by low doses of L-T4 was associated with a failure in the devel...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Thapliyal JP,Gupta BB

    更新日期:1984-07-01 00:00:00

  • Cultured pituitary cell GtH response to GnRH at different stages of rainbow trout oogenesis and influence of steroid hormones.

    abstract::In rainbow trout, a variable in vivo pituitary sensitivity to GnRH has been previously observed, depending on the stage of oogenesis. The purpose of the present work was to study, in vitro, the role of oestradiol (E2) and 17 alpha-hydroxy,20 beta-dihydroprogesterone (17 alpha 20 beta P), respectively, involved in vite...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Weil C,Marcuzzi O

    更新日期:1990-09-01 00:00:00

  • Cells in the intestinal system of holothurians (Echinodermata) express cholecystokinin-like immunoreactivity.

    abstract::The presence of cholecystokinin (CCK), originally isolated from porcine small intestine, has been reported in a diversity of invertebrates ranging, from cnidarians to protochordates, but so far, not in echinoderms. We have used immunohistochemical techniques to demonstrate the presence of cells expressing CCK-like imm...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: García-Arrarás JE,Torres-Avillán I,Ortíz-Miranda S

    更新日期:1991-08-01 00:00:00

  • TRH receptors in fish.

    abstract::The brains of goldfish and other teleosts contain high-affinity binding sites for [3H][3-methyl-His2]thyrotropin-releasing hormone [( 3H]MeTRH) which closely resemble TRH receptors in mammalian brain and pituitary gland in apparent dissociation constant (KD = 3-4 nM), in pharmacology for eight TRH analogs, and in exhi...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Burt DR,Ajah MA

    更新日期:1984-01-01 00:00:00

  • Thyroid hormone deiodinase type 2 mRNA levels in sea lamprey (Petromyzon marinus) are regulated during metamorphosis and in response to a thyroid challenge.

    abstract::Thyroid hormones (THs) are crucial for normal vertebrate development and are the one obligate morphogen that drives amphibian metamorphosis. However, contrary to other metamorphosing vertebrates, lampreys exhibit a sharp drop in serum TH early in metamorphosis, and anti-thyroid agents such as potassium perchlorate (KC...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Stilborn SS,Manzon LA,Schauenberg JD,Manzon RG

    更新日期:2013-03-01 00:00:00

  • Growth hormone-releasing hormone stimulates GH release while inhibiting ghrelin- and sGnRH-induced LH release from goldfish pituitary cells.

    abstract::Goldfish GH-releasing hormone (gGHRH) has been recently identified and shown to stimulate GH release in goldfish. In goldfish, neuroendocrine regulation of GH release is multifactorial and known stimulators include goldfish ghrelin (gGRLN19) and salmon gonadotropin-releasing hormone (sGnRH), factors that also enhance ...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Grey CL,Chang JP

    更新日期:2013-06-01 00:00:00

  • Relationships among hormonal changes, cuticular hydrocarbons, and attractiveness during the first gonadotropic cycle of the female Calliphora vomitoria (Diptera).

    abstract::Attractiveness in adult females of Calliphora vomitoria is correlated with ovarian development and there is a marked increase during the previtellogenic and vitellogenic periods. The development of attractiveness may result from the combined actions of ecdysteroids and juvenile hormone. A rise in total hydrocarbons pa...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Trabalon M,Campan M,Porcheron P,Clement JL,Baehr JC,Moriniere M,Joulie C

    更新日期:1990-11-01 00:00:00

  • Role of the midgut gland in metabolism and excretion of ecdysteroids by lobsters, Homarus americanus.

    abstract::The chromatographic profile of ecdysteroids (Ecds) from the midgut gland (MG) of juvenile female lobsters, Homarus americanus, was examined using high-performance liquid chromatography (HPLC) and radioimmunoassay (RIA) over four stages of the molt cycle. Upon initial examination, highly polar Ecd conjugates appeared t...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Snyder MJ,Chang ES

    更新日期:1992-02-01 00:00:00

  • [Establishment of a map of neurons in the brain of Eisenia fetida (Annelida, Oligochaeta) containing substances immunologically specific to vertebrate peptides].

    abstract::Several antisera specific to neuropeptides of vertebrates were applied to sections of the earthworm Eisenia fetida Sav. Some of them were able to reveal specific neurones of the supraoesophageal ganglion: h-GHRF, CRF, Leu- and Met-enkephalin, dynorphin, substance P, CCK-8, and CCK-8S. A map of different areas or nucle...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Lkhider M,Marcel R,Tramu G

    更新日期:1987-03-01 00:00:00

  • Isolation and structural characterization of proglucagon-derived peptides, pancreatic polypeptide, and somatostatin from the urodele Amphiuma tridactylum.

    abstract::The expression of the preproglucagon gene in vertebrates is markedly species- and tissue-dependent. Three peptides derived from the posttranslational processing of preproglucagon were isolated from an extract of the pancreas of the urodele Amphiuma tridactylum (threetoed amphiuma). The primary structures of the peptid...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Cavanaugh ES,Nielsen PF,Conlon JM

    更新日期:1996-01-01 00:00:00

  • Fecal glucocorticoid response to environmental stressors in green iguanas (Iguana iguana).

    abstract::Quantification of glucocorticoid metabolites in feces has been shown to be a powerful tool in evaluating well-being in vertebrates. Little is known however about the hypothalamic-pituitary-adrenal axis response to stressors, and consequent glucocorticoid excretion, in reptiles. In a longitudinal study, fecal corticost...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Kalliokoski O,Timm JA,Ibsen IB,Hau J,Frederiksen AM,Bertelsen MF

    更新日期:2012-05-15 00:00:00

  • Effectiveness of somatostatin in regulating pancreatic splenic lobe hormone secretion following 99% pancreatectomy in adult chickens.

    abstract::In vivo studies were carried out in adult chickens in an attempt to evaluate the effectiveness of somatostatin (SRIF) in regulating hormone secretion from the splenic pancreatic lobe after 99% of the pancreatic mass was surgically ablated. Sixteen days after either sham operation or 99% pancreatectomy, birds were infu...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Cieslak SR,Hazelwood RL

    更新日期:1986-08-01 00:00:00

  • Glucagon- and NPY-related peptide-immunoreactive cells in the gut of sea bass (Dicentrarchus labrax L.): a light and electron microscopic study.

    abstract::Glucagon and peptide of the neuropeptide Y (NPY) family immunoreactivities were studied in the gut of sea bass (Dicentrarchus labrax) using antisera against bovine/porcine glucagon, porcine glucagon, glicentin (10-30), bovine pancreatic polypeptide (PP), peptide tyrosine tyrosine (PYY), salmon PYY (sPYY), and NPY. Glu...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Gómez-visus I,García-Hernández MP,Lozano MT,Agulleiro B

    更新日期:1998-10-01 00:00:00

  • The distribution of lysine vasopressin (lysipressin) in placental mammals: a reinvestigation of the Hippopotamidae (Hippopotamus amphibius) and Tayassuidae (Tayassu angulatus) families.

    abstract::The neurohypophyseal hormones of the hippopotamus (Hippopotamus amphibius) and collared peccary (Tayassu angulatus) were isolated by molecular sieving and preparative high-pressure liquid chromatography (HPLC). Oxytocin and arginine vasopressin have been identified by their amino acid compositions and their retention ...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Rouille Y,Chauvet MT,Chauvet J,Acher R,Hadley ME

    更新日期:1988-09-01 00:00:00

  • Estradiol and testosterone modulate the tissue-specific expression of ghrelin, ghs-r, goat and nucb2 in goldfish.

    abstract::Ghrelin, and nesfatin-1 (encoded by nucleobindin2/nucb2) are two metabolic peptides with multiple biological effects in vertebrates. While sex steroids are known to regulate endogenous ghrelin and NUCB2 in mammals, such actions by steroids in fish remain unknown. This study aimed to determine whether estradiol (E2) an...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Bertucci JI,Blanco AM,Canosa LF,Unniappan S

    更新日期:2016-03-01 00:00:00

  • Effects of bovine TSH on the tissue thyroxine level and metamorphosis in prometamorphic flounder larvae.

    abstract::Bovine thyroid-stimulating hormone (TSH) was microinjected (0.2 microliter/fish) into prometamorphic flounder larvae and the effects on metamorphosis as well as the tissue thyroxine (T4) and triiodothyronine (T3) levels were studied. After a single injection of TSH (5 mIU/g), the tissue T4 concentration increased mark...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Inui Y,Tagawa M,Miwa S,Hirano T

    更新日期:1989-06-01 00:00:00

  • GTH I and GTH II secretion profiles during the reproductive cycle in female rainbow trout: relationship with pituitary responsiveness to GnRH-A stimulation.

    abstract::The recent purification of two gonadotropins, GTH I and GTH II, in teleost fish and the development of their specific radioimmunoassays using antibodies directed against their beta subunits have demonstrated that earlier assays for GTH II also measured GTH I. Most of the results on the gonadotropic control of reproduc...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Breton B,Govoroun M,Mikolajczyk T

    更新日期:1998-07-01 00:00:00

  • Life cycle exposure of the frog Silurana tropicalis to arsenate: Steroid- and thyroid hormone-related genes are differently altered throughout development.

    abstract::Arsenic contaminates water surface and groundwater worldwide. Several studies have suggested that arsenic acts as an endocrine disruptor in mammalian and non-mammalian species, although its chronic effect during development remains largely unknown. To address this question, life cycle exposures to 0, 0.3 and 0.8ppm of...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Gibson LA,Koch I,Reimer KJ,Cullen WR,Langlois VS

    更新日期:2016-08-01 00:00:00

  • Identification and quantification of 5α-dihydrotestosterone in the teleost fathead minnow (Pimephales promelas) by gas chromatography-tandem mass spectrometry.

    abstract::The steroid hormone 5α-dihydrotestosterone (DHT) is one of the most physiologically important androgens in male vertebrates, with the exception of teleost fish, in which it is generally assumed that DHT does not play any major physiological role. However, this assumption is challenged by the fact that all the componen...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Margiotta-Casaluci L,Courant F,Antignac JP,Le Bizec B,Sumpter JP

    更新日期:2013-09-15 00:00:00

  • Involvement of Gαs-proteins in the action of relaxin-like gonad-stimulating substance on starfish ovarian follicle cells.

    abstract::Gonad-stimulating substance (GSS) in starfish is the only known invertebrate peptide hormone responsible for final gamete maturation, rendering it functionally analogous to gonadotropins in vertebrates. In breeding season (stage V), GSS stimulates oocyte maturation to induce 1-methyladenine (1-MeAde) by ovarian follic...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Mita M,Haraguchi S,Watanabe M,Takeshige Y,Yamamoto K,Tsutsui K

    更新日期:2014-09-01 00:00:00

  • Production and characterization of monoclonal antibodies against recombinant tethered follicle-stimulating hormone from Japanese eel Anguilla japonica.

    abstract::We prepared monoclonal antibodies (mAbs) against a recombinant tethered follicle-stimulating hormone (rec-FSH) from Japanese eel Anguilla japonica that was produced in Escherichia coli. Positive hybridomas (clones eFA-C5, eFA-C10, eFA-C11, eFA-C12, eFA-C13, and eFB-C14) were selected by using the eel FSH antigen in EL...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Kim DJ,Park CW,Kim DW,Park HK,Byambaragchaa M,Lee NS,Hong SM,Seo MY,Kang MH,Min KS

    更新日期:2016-07-01 00:00:00

  • Effects of dietary arachidonic acid on the reproductive physiology of female Atlantic cod (Gadus morhua L.).

    abstract::The present study was designed to investigate potential effects of arachidonic acid (ARA) on the reproductive physiology of female Atlantic cod (Gadus morhua L.). Two-year old Atlantic cod of both sexes were equally distributed into eight sea cages after completion of their first spawning in May 2005. Four experimenta...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Norberg B,Kleppe L,Andersson E,Thorsen A,Rosenlund G,Hamre K

    更新日期:2017-09-01 00:00:00

  • Induction of male-type gonadotropin secretion by implantation of 11-ketotestosterone in female goldfish.

    abstract::In goldfish, plasma gonadotropin levels increase during spawning in both males and females (GTH surge). A female-typical GTH surge induces ovulation (ovulatory surge), and a male-typical surge triggers milt production in response to sex pheromones released from ovulatory females. This study examined whether the male-t...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Kobayashi M,Furukawa K,Kim MH,Aida K

    更新日期:1997-12-01 00:00:00