Platelet activation and interaction with leucocytes in patients with sepsis or multiple organ failure.


:This study focuses on the role of platelet membrane glycoproteins and platelet-leucocyte adhesion in patients with sepsis and multiple organ failure (MOF). Specifically, the study raises the following issues: (1) the influence of sepsis and MOF on platelet activation as assessed by surface expression of platelet membrane glycoproteins GPIIb-IIIa and thrombospondin; and (2) the effect of sepsis and MOF on platelet adhesion to circulating leucocytes. In addition, platelet activation and platelet-leucocyte adhesion are evaluated according to clinical outcome. Forty-five patients with suspected sepsis or MOF were evaluated by intensive care scoring systems (APACHE II and Elebute) to assess severity of disease. Flow cytometric techniques were used to examine platelet membrane expression of various adhesion molecules on circulating platelets and the appearance of platelet specific antigen (CD41) on leucocytes as an index of platelet-leucocyte adhesion. The results were compared with severity of disease and according to outcome in patients. Twenty-eight patients of the total study population were septic and 17 were non-septic. Twenty-two of the 28 septic patients suffered from severe MOF (APACHE II > or = 20) whereas in six septic patients MOF was absent. Eleven of the non-septic group suffered from moderate MOF whereas in six, severe MOF was present. In septic patients fibrinogen receptor activity on platelets was significantly above normal values (P < 0.001). When MOF was present, thrombospondin surface expression on circulating platelets also increased significantly (P < 0.05). Concomitantly, platelet-leucocyte adhesion was increased in sepsis (P < 0.05) and decreased in patients with MOF (P < 0.05). Significant lower levels of circulating platelet-leucocyte aggregates occurred in non-survivors (P < 0.05). We conclude that sepsis is associated with increased surface expression of platelet adhesion molecules and an increased occurrence of circulating platelet-leucocyte aggregates. The decrease in circulating platelet-leucocyte peripheral sequestration. An increased platelet-leucocyte adhesion and sequestration might account for development of MOF in the course of sepsis.


Eur J Clin Invest


Gawaz M,Fateh-Moghadam S,Pilz G,Gurland HJ,Werdan K




Has Abstract


1995-11-01 00:00:00












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    authors: Vesely BA,Song S,Sanchez-Ramos J,Fitz SR,Alli AA,Solivan SM,Gower WR Jr,Vesely DL

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    authors: Dewit O,Starkel P,Roblin X

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    更新日期:1985-06-01 00:00:00

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    更新日期:1977-02-01 00:00:00

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    更新日期:1996-11-01 00:00:00

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    更新日期:1996-10-01 00:00:00

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    更新日期:1984-08-01 00:00:00

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    更新日期:1990-08-01 00:00:00

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    journal_title:European journal of clinical investigation

    pub_type: 杂志文章


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    journal_title:European journal of clinical investigation

    pub_type: 杂志文章,多中心研究


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    journal_title:European journal of clinical investigation

    pub_type: 杂志文章


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    更新日期:1990-02-01 00:00:00

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    journal_title:European journal of clinical investigation

    pub_type: 杂志文章


    authors: van Trijp MJ,Bos WJ,van der Schouw YT,Muller M,Grobbee DE,Bots ML

    更新日期:2005-10-01 00:00:00

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    journal_title:European journal of clinical investigation

    pub_type: 临床试验,杂志文章,随机对照试验


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    更新日期:1995-02-01 00:00:00

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    journal_title:European journal of clinical investigation

    pub_type: 杂志文章,评审


    authors: Scorrano L

    更新日期:2013-08-01 00:00:00

  • Accuracy of prehospital point-of-care lactate in early in-hospital mortality.

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    journal_title:European journal of clinical investigation

    pub_type: 杂志文章


    authors: Martín-Rodríguez F,López-Izquierdo R,Medina-Lozano E,Ortega Rabbione G,Del Pozo Vegas C,Carbajosa Rodríguez V,Castro Villamor MÁ,Sánchez-Soberon I,Sanz-García A

    更新日期:2020-12-01 00:00:00