IL-6 secretion by human peritoneal mesothelial and ovarian cancer cells.


:Malignant ascites of epithelial ovarian cancer patients contains high levels of interleukin 6 (IL-6). The present study was conducted to compare the secretion of IL-6 by seven different human ovarian cancer cell lines (OVCA) and cultured human peritoneal mesothelial cells (HPMC) and to examine the regulation of its production by other cytokines. IL-6 was detected in supernatant medium of all mesothelial cell cultures (8/8) and 6/7 ovarian cancer cell lines. Levels of IL-6 secreted by HPMC (median 27,100 pg/1 x 10(5) cells; range 3870-168,200) were 590-fold higher (P < 0.01) than those secreted by OVCA (median 46 pg/1 x 10(5) cells; range 0-16,450). Treatment with TNF-alpha or IL-1 beta (both 10 ng/ml) for both types of cells and both cytokines resulted in a significant (P < 0.05) elevation of IL-6 production. In OVCA IL-6 secretion was increased 7- and 39-fold and in HPMC 6- and 8-fold, respectively. Under TNF-alpha treatment IL-6-levels secreted by HPMC were 149-fold higher (P < 0.01) than those generated by OVCA. Similarly, IL-1 beta-induced IL-6 levels were 102-fold higher in HPMC (median 288,800 pg/1 x 10(5) cells; range 93,125-552,800) than in OVCA. IFN-gamma (10 ng/ml) increased IL-6 generation in OVCA (6-fold) but not HPMC. The proliferation of both cell types however, was significantly (P < 0.05) inhibited by IFN-gamma. Our results suggest that peritoneal mesothelial cells may be a prominent source of IL-6 in ovarian cancer-related ascites.






Offner FA,Obrist P,Stadlmann S,Feichtinger H,Klingler P,Herold M,Zwierzina H,Hittmair A,Mikuz G,Abendstein B




Has Abstract,Author List Incomplete


1995-08-01 00:00:00
















  • Molecular cloning, characterization and expression analysis of an IL-21 homologue from Tetraodon nigroviridis.

    abstract::Interleukin-21 (IL-21) is an important immune cytokine that was well characterized in human and mammals, but little is known in fish. In present study, an IL-21 homologue was cloned and well characterized from Tetraodon nigroviridis. The full-length Tetraodon IL-21 cDNA was 849bp in size, containing an open reading fr...


    pub_type: 杂志文章


    authors: Wang HJ,Xiang LX,Shao JZ,Jia S

    更新日期:2006-08-01 00:00:00

  • CK2 induced RIG-I drives metabolic adaptations in IFNγ-treated glioma cells.

    abstract::Given the known anti-tumorigenic properties of IFNγ, its effect on glioma cell survival was investigated. Though IFNγ had no effect on glioma cell viability, it induced cell cycle arrest. This was accompanied by increased expression of p53 and retinoic acid inducible gene (RIG-I). While RIG-I had no effect on glioma c...


    pub_type: 杂志文章


    authors: Ghildiyal R,Sen E

    更新日期:2017-01-01 00:00:00

  • G-CSF-induced evacuation of sinusoidal NK cells and the facilitation of liver regeneration in a partial hepatectomy.

    abstract::Since liver regeneration after partial hepatectomy (PHx) is known to improve by pretreatment with recombinant human G-CSF (rhG-CSF), we investigated the mechanism by evaluating the distribution and activity of sinusoidal NK cells. F344 rats were treated with rhG-CSF (250 microg/kg/day) for 5 days before PHx. Pretreatm...


    pub_type: 杂志文章


    authors: Oishi K,Hayamizu K,Aihaiti X,Itamoto T,Arihiro K,Asahara T

    更新日期:2006-04-01 00:00:00

  • Predictive value of low interleukin-33 in critically ill patients.

    abstract::Patients admitted to a medical intensive care unit (ICU) are characterized by an activated immune system and exhibit a high mortality rate irrespective of the underlying cause of admission. Interleukin (IL)-33 has been shown to be protective in experimental sepsis models and it has been demonstrated that circulating l...


    pub_type: 临床试验,杂志文章


    authors: Krychtiuk KA,Stojkovic S,Lenz M,Brekalo M,Huber K,Wojta J,Heinz G,Demyanets S,Speidl WS

    更新日期:2018-03-01 00:00:00

  • Interferon gamma and prostaglandin in BSE-infected cattle.

    abstract::Suspect field cases of BSE infection (displaying clinical signs) were examined for possible alterations of cytokine/autacoid plasma levels and were compared to control cases (not displaying clinical signs of BSE infection). Interferon gamma (IFN-gamma) plasma levels were demonstrated as being elevated in all suspected...


    pub_type: 杂志文章


    authors: Murphy C,Breen C,Rogers M,Giese M

    更新日期:2001-02-07 00:00:00

  • The IL-17 G-152A single nucleotide polymorphism is associated with pulmonary tuberculosis in northern Spain.

    abstract:BACKGROUND:Interleukin 17 (IL-17) is induced during the early stages of tuberculosis infection, playing an important role in the defense against mycobacterial infection. To date, only one study performed in Chinese Han population has found an association between IL-17F sequence variants and susceptibility to tuberculos...


    pub_type: 杂志文章


    authors: Ocejo-Vinyals JG,de Mateo EP,Hoz MÁ,Arroyo JL,Agüero R,Ausín F,Fariñas MC

    更新日期:2013-10-01 00:00:00

  • Association of interleukin (IL)-4 variable number of tandem repeats (VNTRs) and IL-4-590 promoter polymorphisms with susceptibility to pediatric autoimmune hepatitis type 1.

    abstract:OBJECTIVE:Autoimmune hepatitis (AIH) is a chronic inflammatory liver disease mediated by an autoimmune reaction to hepatocytes, the present study aimed to assess the possible associations between interleukin-4 (IL-4) variable number of tandem repeats (VNTRs) and IL-4-590 promoter polymorphisms and susceptibility to aut...


    pub_type: 杂志文章


    authors: Mansour AI,Behairy OG,Abd Almonaem ER,Abd-Rabuh RM,Ahmed IAE

    更新日期:2018-10-01 00:00:00

  • Regulation of transforming growth factor-beta secretion by human peritoneal mesothelial and ovarian carcinoma cells.

    abstract::This study was conducted to compare the secretion of TGF-beta isoforms by human ovarian carcinoma (OVCA) cell lines (n=12) and human peritoneal mesothelial cells (HPMC;n=6) and to examine the regulation of their production by inflammatory cytokines. TGF-beta isoforms were furthermore analysed in OVCA-associated asciti...


    pub_type: 杂志文章


    authors: Abendstein B,Stadlmann S,Knabbe C,Buck M,Müller-Holzner E,Zeimet AG,Marth C,Obrist P,Krugmann J,Offner FA

    更新日期:2000-07-01 00:00:00

  • Regulation and role of interleukin 6 in wounded human epithelial keratinocytes.

    abstract::Dermal wounding is accompanied by inflammation and the resulting proinflammatory cytokines, including interleukin (IL)-6, are thought to play an important role in the repair process. IL-6 is produced by normal human keratinocytes to various dermatological diseases and we have recently shown it is also required for nor...


    pub_type: 杂志文章


    authors: Sugawara T,Gallucci RM,Simeonova PP,Luster MI

    更新日期:2001-09-21 00:00:00

  • Hypocholesterolemia and dysregulated production of angiopoietin-like proteins in sickle cell anemia patients.

    abstract::Angiopoietin-like proteins (ANGPTL) are responsible for inhibiting lipoprotein lipase activity, and ANGPTL3 and ANGPTL4 deficiencies have been shown to lower lipoprotein levels in animal models and in humans carrying loss-of-function mutations. Sickle cell anemia (SCA) is a hereditary hemolytic anemia characterized by...


    pub_type: 杂志文章


    authors: Vendrame F,Olops L,Saad STO,Costa FF,Fertrin KY

    更新日期:2019-08-01 00:00:00

  • Direct contact with stimulated T cells induces the expression of IL-1beta and IL-1 receptor antagonist in human monocytes. Involvement of serine/threonine phosphatases in differential regulation.

    abstract::Imbalance in the production of cytokines and their inhibitors plays a part in inflammation in chronic destructive diseases. Direct cell-cell contact with stimulated T cells markedly induces the production of pro-inflammatory cytokines and matrix metalloproteinases in monocytes. This study demonstrates that direct cont...


    pub_type: 杂志文章


    authors: Vey E,Dayer JM,Burger D

    更新日期:1997-07-01 00:00:00

  • Mouse genetic background influences severity of immune responses following trauma-hemorrhage.

    abstract::Studies have shown that following bacterial infection or endotoxin administration, immune functions are regulated differently in mice of different genetic background. Since the susceptibility to sepsis following trauma-hemorrhage is dependant on the severity of injury, it is important to determine whether genetic back...


    pub_type: 杂志文章


    authors: Matsutani T,Anantha Samy TS,Kang SC,Bland KI,Chaudry IH

    更新日期:2005-05-21 00:00:00

  • Low concentrations of recombinant granulocyte macrophage-colony stimulating factor derived from Chinese hamster ovary cells augments long-term bioactivity with delayed clearance in vitro.

    abstract::To date, the biological activity of granulocyte macrophage-colony stimulating factor (GM-CSF) has been investigated by using mostly Escherichia coli- or yeast cell-derived recombinant human GM-CSF (erhGM-CSF and yrhGM-CSF, respectively). However, Chinese hamster ovary cell-derived recombinant human GM-CSF (crhGM-CSF),...


    pub_type: 杂志文章


    authors: Hashimoto A,Tanaka T,Itoh Y,Yamagata A,Kitamura N,Tazawa R,Nakagaki K,Nakata K

    更新日期:2014-08-01 00:00:00

  • Change in the accessibility of an epitope of the human granulocyte-colony stimulating factor after binding to receptors.

    abstract::In a previous work we demonstrated that monoclonal antibody (mAb) 8C2 recognized a human granulocyte-colony stimulating factor (hG-CSF) region left unmasked after binding to placenta receptors, whereas mAb 6E3 defined a receptor-buried epitope. Herein we examined the role of these antigenic regions on the proliferativ...


    pub_type: 杂志文章


    authors: Marino VJ,Sterin-Prync AE,Roguin LP

    更新日期:2003-04-01 00:00:00

  • Glycosaminoglycans alter the conformation of interferon-gamma.

    abstract::Interferon-gamma (IFN-gamma) is a potent immunomodulatory cytokine whose physiological roles are modulated by the extracellular matrix. Here, circular dichroism and fluorescence spectroscopic techniques are used to demonstrate that low molecular weight heparin and chondroitin sulfate cause significant changes in secon...


    pub_type: 杂志文章


    authors: Balasubramanian V,Ramanathan M

    更新日期:2000-05-01 00:00:00

  • Effect of psoriasis activity on VEGF and its soluble receptors concentrations in serum and plaque scales.

    abstract::Vascular endothelial growth factor (VEGF) demonstrating pro-angiogenic activity promote new blood vessel formation in psoriatic lesions. The aim of this study was to evaluate the serum concentrations of VEGF, its soluble receptors (sVEGF R1 and R2) and VEGF content in scales of patients with psoriasis. To analyze poss...


    pub_type: 杂志文章


    authors: Flisiak I,Zaniewski P,Rogalska M,Myśliwiec H,Jaroszewicz J,Chodynicka B

    更新日期:2010-12-01 00:00:00

  • Inhibition of cytokine activation processes in vitro by tenidap, a novel anti-inflammatory agent.

    abstract::Tenidap is a novel anti-inflammatory and anti-arthritic agent that lowers intracellular pH and suppresses anion transport when applied to cells in vitro. Both of these parameters are known to influence pro-inflammatory cell function. To investigate whether tenidap can modulate cellular responses to cytokine stimulatio...


    pub_type: 杂志文章


    authors: McNiff PA,Laliberte RE,Svensson L,Pazoles CJ,Gabel CA

    更新日期:1995-02-01 00:00:00

  • Expression of canine interleukin-4 in canine chondrocytes inhibits inflammatory cascade through STAT6.

    abstract::Interleukin-4 (IL-4) is a pleiotropic cytokine with broad spectrum of biological effects on target cells. This study deals with the mammalian expression of canine IL-4 (cIL-4) in canine articular chondrocytes (CAC) and its ability to down-regulate pro-inflammatory cytokines, enzyme mediators and their catabolites. We ...


    pub_type: 杂志文章


    authors: Rachakonda PS,Rai MF,Manning K,Schmidt MF

    更新日期:2008-10-01 00:00:00

  • Differences in the inducible gene expression and protein production of IL-12p40, IL-12p70 and IL-23: involvement of p38 and JNK kinase pathways.

    abstract::The proper balance between IL-12p40-related cytokines controls the appearance of normal and pathological Th1 immune responses. In this study, we examined the inducible IL-12p40, IL-12p35 and IL-23p19 mRNA expression and protein production in human peripheral blood mononuclear cells (PBMC) and purified monocytes, isola...


    pub_type: 杂志文章


    authors: Dobreva ZG,Stanilova SA,Miteva LD

    更新日期:2008-07-01 00:00:00

  • Cytokines profile in serum of homozygous familial hypercholesterolemia is changed by LDL-apheresis.

    abstract:OBJECTIVE:The effects of LDL-apheresis (LDLa) with dextran sulphate on plasma cytokines in 6 homozygous familial hypercholesterolemic (HozFH) patients, were evaluated. METHODS:Plasma IL-1α; IL-1ra; IL-4; IL-6; IL-10; IL-12(p40); IL-12(p70); TNF-α, sTNF-R, VEGF, VEGF-R1, E-Selectin (ESEL), and P-Selectin (PSEL) concent...


    pub_type: 杂志文章


    authors: Stefanutti C,Vivenzio A,Di Giacomo S,Ferraro PM

    更新日期:2011-08-01 00:00:00

  • History of interleukin-4.

    abstract::The history of the discovery and the development of our knowledge of IL-4 exemplifies the path of progress in biomedical science. There are unanticipated twists and turns although progress is made, sometimes quickly, other times far too slowly. Illustrative is the extended time from the first report of IL-4 in 1982 to...


    pub_type: 杂志文章,评审


    authors: Paul WE

    更新日期:2015-09-01 00:00:00

  • Production of tumour necrosis factor alpha by primary cultured rat alveolar epithelial cells.

    abstract::Tumour necrosis factor alpha(TNF-alpha) is one of the most important pro-inflammatory cytokines, which plays an important role in host defense and acute inflammation related to tissue injury. The major source of TNF-alpha has been shown to be immune cells such as macrophages and neutrophils. In the present study, we d...


    pub_type: 杂志文章


    authors: McRitchie DI,Isowa N,Edelson JD,Xavier AM,Cai L,Man HY,Wang YT,Keshavjee SH,Slutsky AS,Liu M

    更新日期:2000-06-01 00:00:00

  • TNF-alpha and melphalan modulate a specific group of early expressed genes in a murine melanoma model.

    abstract:BACKGROUND:Cutaneous melanoma displays high morbidity and mortality rates. Isolated limb perfusion with melphalan (Mel) is used for the treatment of non-resectable, locally advanced extremity melanomas. When combined with tumor necrosis factor alpha (TNF-alpha) treatment, the complete response varies between 70% and 90...


    pub_type: 杂志文章


    authors: de Lima VC,de Carvalho AF,Morato-Marques M,Hashimoto VL,Spilborghs GM,Marques SM,Landman G,Torres C,Braga Ribeiro K,Brentani H,Reis LF,Dias AA

    更新日期:2013-05-01 00:00:00

  • CXC chemokine receptor 4 signaling upon co-activation with stromal cell-derived factor-1α and ubiquitin.

    abstract::Recently, we reported that extracellular ubiquitin functions as another agonist of CXC chemokine receptor (CXCR)4. Whereas the cognate CXCR4 ligand, stromal cell-derived factor (SDF)-1α, is also a CXCR7 agonist, ubiquitin does not bind to CXCR7. Because both ligands are present in the extracellular environment, co-act...


    pub_type: 杂志文章


    authors: Tripathi A,Davis JD,Staren DM,Volkman BF,Majetschak M

    更新日期:2014-02-01 00:00:00

  • Tumor necrosis factor-α and interleukin-1β gene polymorphisms and risk of brain abscess in North Indian population.

    abstract:OBJECTIVE:Brain abscess develops in response to a parenchymal infection with pyogenic bacteria. Tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) are the most crucial pro-inflammatory cytokines with both beneficial and destructive properties for the central nervous system. The present study evaluated the assoc...


    pub_type: 杂志文章


    authors: Mishra P,Prasad KN,Singh K,Bajpai A,Sahu RN,Ojha BK

    更新日期:2015-09-01 00:00:00

  • Changes in immune-endocrine response after surgery.

    abstract::The authors evaluated the relationship between hormonal and cytokine responses after surgery. Patients who underwent thoracic oesophagectomy (O group; n = 7), pulmonary lobectomy (P group; n = 5), modified mastectomy (M group; n = 5) and laparoscopic cholecystectomy (LC group; n = 7), were randomly selected. Circulati...


    pub_type: 杂志文章


    authors: Yamauchi H,Kobayashi E,Yoshida T,Kiyozaki H,Hozumi Y,Kohiyama R,Suminaga Y,Sakurabayashi I,Fujimura A,Miyata M

    更新日期:1998-07-01 00:00:00

  • Association of serum amyloid A levels with adipocyte size and serum levels of adipokines: differences between men and women.

    abstract::The aim of this study was to characterize the association between adipocyte enlargement and circulating levels of serum amyloid A (SAA). Furthermore, we wanted to search for possible associations with measures of glycemic control and levels of circulating adipokines and/or inflammatory markers in men and women with a ...


    pub_type: 杂志文章


    authors: Sjöholm K,Lundgren M,Olsson M,Eriksson JW

    更新日期:2009-12-01 00:00:00

  • Association of TNF-α but not IL-1β levels with the presence of Helicobacter pylori infection increased the risk of peptic ulcer development.

    abstract::Peptic ulcer is a lesion in the mucosa of the digestive tract affecting many people all around the world. Recent investigations have indicated that produced inflammatory cytokines such as TNF-α and IL-1β in response to gastric infection by Helicobacter pylori play an important role in the development of peptic ulcer. ...


    pub_type: 杂志文章


    authors: Tourani M,Habibzadeh M,Karkhah A,Shokri-Shirvani J,Barari L,Nouri HR

    更新日期:2018-10-01 00:00:00

  • Interleukin-12 as an adjuvant for induction of protective antibody responses.

    abstract::Interleukin (IL)-12 is a pivotal cytokine that strongly stimulates Th1-associated cellular immunity. It is now recognized that IL-12 also activates humoral immunity to both T-dependent and T-independent antigens. This has let to considerable interest in exploiting IL-12 as a vaccine adjuvant for protection against var...


    pub_type: 杂志文章,评审


    authors: Metzger DW

    更新日期:2010-10-01 00:00:00

  • Unique cytokine secretion profile in children with both type I diabetes and asthma distinct from that of solely diabetic or asthmatic children.

    abstract::Asthma and type I diabetes are major causes of chronic illness in childhood which, according to the current paradigm, have mutually antagonistic immunopathologies. Nonetheless, the disorders appear to preferably coexist both on population and individual levels. To assess whether children with asthma and type I diabete...


    pub_type: 杂志文章


    authors: Kainonen E,Rautava S,Korkeamäki M,Isolauri E

    更新日期:2006-05-01 00:00:00